LOCUS       ArpC1_pLib              6049 bp ds-DNA     circular     03-SEP-2021
DEFINITION  .
ACCESSION   pLIB                                                               
SOURCE      Unknown.                                                           
  ORGANISM  Unknown Unclassified.                                              
FEATURES             Location/Qualifiers
     CDS             687..1547
                     /label="AmpR CDS"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_signal     2611..2835
                     /label="Tn7R misc_signal"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     CDS             complement(2902..3435)
                     /label="GentaR CDS"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_signal     4048..4081
                     /label="LoxP misc_signal"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     misc_signal     4105..4131
                     /label="Cas_for misc_signal"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     primer          4105..4131
                     /label="Cas1_for (alpha)"
                     /note="sequence: AACGCTCTATGGTCTAAAGATTTAAATCGACCTACTCCGGAATATTAATAGATC"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_signal     4132..4162
                     /label="Polh Promoter misc_signal"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_signal     4221..4223
                     /label="mut. polh start misc_signal"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    4298..5413
                     /label="ArpC1"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     CDS             4298..5428
                     /label="Translation 4298-5428"
     misc_feature    5414..5416
                     /label="stop"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_signal     5497..5688
                     /label="SV40 polyA misc_signal"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_signal     complement(5765..5788)
                     /label="Cas_rev misc_signal"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     primer          complement(5765..5788)
                     /label="CasW_rev (omega)"
                     /note="sequence: AACCCCGATTGAGATATAGATTTATTTAAATGGTTATGATAGTTATTGCTCAGCG"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_signal     5802..5967
                     /label="Tn7L misc_signal"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
ORIGIN
        1 ttctctgtca cagaatgaaa atttttctgt catctcttcg ttattaatgt ttgtaattga
       61 ctgaatatca acgcttattt gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc
      121 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct
      181 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg
      241 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga
      301 ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt
      361 ttttcgccct ttgacgttgg agtccacgtt cttaatagtg gactcttgtt ccaaactgga
      421 acaacactca accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg
      481 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata
      541 ttaacgctta caatttaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
      601 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
      661 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
      721 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
      781 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
      841 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag
      901 ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc
      961 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta
     1021 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg
     1081 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca
     1141 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
     1201 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat
     1261 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg
     1321 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata
     1381 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta
     1441 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
     1501 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag
     1561 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
     1621 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
     1681 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     1741 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     1801 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     1861 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     1921 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     1981 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     2041 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     2101 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     2161 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     2221 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     2281 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     2341 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
     2401 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
     2461 gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc
     2521 tgtgcggtat ttcacaccgc atagaccagc cgcgtaacct ggcaaaatcg gttacggttg
     2581 agtaataaat ggatgccctg cgtaagcggg tgtgggcgga caataaagtc ttaaactgaa
     2641 caaaatagat ctaaactatg acaataaagt cttaaactag acagaatagt tgtaaactga
     2701 aatcagtcca gttatgctgt gaaaaagcat actggacttt tgttatggct aaagcaaact
     2761 cttcattttc tgaagtgcaa attgcccgtc gtattaaaga ggggcgtggc caagggcatg
     2821 gtaaagacta tattcgcggc gttgtgacaa tttaccgaac aactccgcgg ccgggaagcc
     2881 gatctcggct tgaacgaatt gttaggtggc ggtacttggg tcgatatcaa agtgcatcac
     2941 ttcttcccgt atgcccaact ttgtatagag agccactgcg ggatcgtcac cgtaatctgc
     3001 ttgcacgtag atcacataag caccaagcgc gttggcctca tgcttgagga gattgatgag
     3061 cgcggtggca atgccctgcc tccggtgctc gccggagact gcgagatcat agatatagat
     3121 ctcactacgc ggctgctcaa acttgggcag aacgtaagcc gcgagagcgc caacaaccgc
     3181 ttcttggtcg aaggcagcaa gcgcgatgaa tgtcttacta cggagcaagt tcccgaggta
     3241 atcggagtcc ggctgatgtt gggagtaggt ggctacgtct ccgaactcac gaccgaaaag
     3301 atcaagagca gcccgcatgg atttgacttg gtcagggccg agcctacatg tgcgaatgat
     3361 gcccatactt gagccaccta actttgtttt agggcgactg ccctgctgcg taacatcgtt
     3421 gctgctgcgt aacatcgttg ctgctccata acatcaaaca tcgacccacg gcgtaacgcg
     3481 cttgctgctt ggatgcccga ggcatagact gtacaaaaaa acagtcataa caagccatga
     3541 aaaccgccac tgcgccgtta ccaccgctgc gttcggtcaa ggttctggac cagttgcgtg
     3601 agcgcatacg ctacttgcat tacagtttac gaaccgaaca ggcttatgtc aactgggttc
     3661 gtgccttcat ccgtttccac ggtgtgcgtc acccggcaac cttgggcagc agcgaagtcg
     3721 aggcatttct gtcctggctg gcgaacgagc gcaaggtttc ggtctccacg catcgtcagg
     3781 cattggcggc cttgctgttc ttctacggca aggtgctgtg cacggatctg cccttgcttc
     3841 aggagatcgg tagacctcgg ccgtcgcggc gcttgccggt ggtgctgacc ccggatgaag
     3901 tggttcgcat cctcggtttt ctggaaggcg agcatcgttt gttcgcccag gactctagct
     3961 atagttctag tggttggcta cagctttgtt tgtactatca acaggttgaa ctgctgatca
     4021 acagatcctc tacgcggccg cggtaccata acttcgtata gcatacatta tacgaagtta
     4081 tctggtttta ctagtatcga ttcgcgacct actccggaat attaatagat catggagata
     4141 attaaaatga taaccatctc gcaaataaat aagtatttta ctgttttcgt aacagttttg
     4201 taataaaaaa acctataaat attccggatt attcataccg tcccaccatc gggCGCGGAT
     4261 CTGCGGCCGC GAATTCCTTG TCCCCGCTcg cggatccATG GCCTATCACT CCTTCTTGGT
     4321 AGAACCTATA AGCTGTCACG CATGGAACAA AGACAGGACG CAAATAGCAA TATGCCCGAA
     4381 CAACCATGAA GTCCACATTT ACGAGAAAAG TGGAGCTAAG TGGACTAAGG TCCATGAACT
     4441 GAAGGAGCAC AATGGCCAAG TAACGGGCAT CGACTGGGCC CCAGAAAGTA ACAGAATCGT
     4501 GACCTGCGGT ACTGACCGTA ATGCGTACGT TTGGACCTTG AAAGGAAGAA CATGGAAACC
     4561 TACGCTGGTA ATTCTCAGGA TTAATCGTGC GGCGAGATGT GTCCGTTGGG CTCCGAATGA
     4621 GAACAAATTT GCTGTAGGTA GCGGAAGTCG TGTGATTAGT ATCTGCTACT TCGAACAAGA
     4681 GAACGACTGG TGGGTTTGTA AACACATTAA AAAACCAATC AGATCCACGG TCCTTTCACT
     4741 GGATTGGCAT CCTAATAACG TACTCCTGGC GGCGGGTTCC TGTGATTTTA AGTGCCGCAT
     4801 CTTCTCCGCC TATATTAAGG AGGTGGAAGA AAGACCAGCC CCAACTCCAT GGGGCAGTAA
     4861 GATGCCTTTC GGCGAACTCA TGTTCGAATC TAGTTCTTCT TGCGGATGGG TTCATGGCGT
     4921 TTGTTTTTCT GCAAGCGGTA GTCGCGTGGC TTGGGTTAGC CACGACAGCA CGGTTTGCCT
     4981 GGCAGACGCA GATAAAAAGA TGGCTGTAGC AACCCTTGCG AGCGAGACTT TGCCACTCTT
     5041 GGCACTCACC TTCATCACTG ACAACTCGCT CGTTGCGGCA GGCCATGATT GCTTTCCGGT
     5101 ACTGTTTACT TACGACGCCG CAGCTGGTAT GTTGTCTTTC GGCGGtCGCC TTGACGTCCC
     5161 CAAGCAGAGC TCTCAGCGCG GTCTCACTGC CCGTGAAAGA TTCCAAAATC TTGATAAGAA
     5221 GGCATCCTCC GAAGGAGGTA CAGCCGCGGG CGCTGGATTG GACAGTCTGC ACAAAAACTC
     5281 GGTGTCCCAA ATATCGGTAT TGTCCGGAGG AAAAGCTAAA TGCAGTCAGT TCTGCACAAC
     5341 GGGAATGGAT GGAGGTATGA GCATTTGGGA CGTCAAAAGC CTCGAATCAG CCCTCAAAGA
     5401 TTTGAAAATT AAAtaaacta gtggtaccaa gcttgtcgag aagtactaga ggatcataat
     5461 cagccatacc acatttgtag aggttttact tgctttaaaa aacctcccac acctccccct
     5521 gaacctgaaa cataaaatga atgcaattgt tgttgttaac ttgtttattg cagcttataa
     5581 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
     5641 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgga tctgatcact
     5701 gcttgagcct agaagatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg
     5761 ccaccgctga gcaataacta tcataacccc taggagatcc gaaccagata agtgaaatct
     5821 agttccaaac tattttgtca tttttaattt tcgtattagc ttacgacgct acacccagtt
     5881 cccatctatt ttgtcactct tccctaaata atccttaaaa actccatttc cacccctccc
     5941 agttcccaac tattttgtcc gcccacagcg gggcattttt cttcctgtta tgtttttaat
     6001 caaacatcct gccaactcca tgtgacaaac cgtcatcttc ggctacttt
//