LOCUS       Exported               11484 bp DNA     circular SYN 01-JUL-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pBID2_UAS>Gateway
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11484)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 2, 2021 from SnapGene Viewer 5.3.1
            https://www.snapgene.com
COMMENT     pBID-UAS-SynR from 1 to 9774
            White eye sequence intron 4332 cac missing
FEATURES             Location/Qualifiers
     source          1..11484
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     rep_origin      complement(135..441)
                     /direction=LEFT
                     /label=F1 ori
     misc_feature    667..4785
                     /label=Mini-White
     misc_feature    4838..5268
                     /label=Gypsy Ins.
                     /label=Gypsy Insulator
     promoter        5314..5790
                     /label=20xUAS
     promoter        5823..5977
                     /label=DSCP
     misc_recomb     6007..7711
                     /label=Gateway Cassette
     misc_feature    7744..8410
                     /label=p10 3'UTR
     misc_feature    8544..8974
                     /label=Gypsy Insulator
                     /label=Gypsy Insulator(1)
     misc_recomb     8987..9271
                     /label=attB PhiC31
                     /label=attB PhiC Integrase
     rep_origin      complement(9719..10401)
                     /direction=LEFT
                     /label=ColE1 origin
     CDS             complement(10499..11158)
                     /label=AmpR
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgaat tgtaatacga ctcactatag ggcgaattgg gtacgtaccg
      661 ggcccctagt atgtatgtaa gttaataaaa cccatttttg cggaaagtag ataaaaaaaa
      721 catttttttt ttttactgca ctggatatca ttgaacttat ctgatcagtt ttaaatttac
      781 ttcgatccaa gggtatttga tgtaccaggt tctttcgatt acctctcact caaaatgaca
      841 ttccactcaa agtcagcgct gtttgcctcc ttctctgtcc acagaaatat cgccgtctct
      901 ttcgccgctg cgtccgctat ctctttcgcc accgtttgta gcgttacgta gcgtcaatgt
      961 ccgccttcag ttgcattttg tcagcggttt cgtgacgaag ctccaagcgg tttacgccat
     1021 caattaaaca caaagtgctg tgccaaaact cctctcgctt cttatttttg tttgtttttt
     1081 gagtgattgg ggtggtgatt ggttttgggt gggtaagcag gggaaagtgt gaaaaatccc
     1141 ggcaatgggc caagaggatc aggagctatt aattcgcgga ggcagcaaac acccatctgc
     1201 cgagcatctg aacaatgtga gtagtacatg tgcatacatc ttaagttcac ttgatctata
     1261 ggaactgcga ttgcaacatc aaattgtctg cggcgtgaga actgcgaccc acaaaaatcc
     1321 caaaccgcaa ttgcacaaac aaatagtgac acgaaacaga ttattctggt agctgttctc
     1381 gctatataag acaatttttg agatcatatc atgatcaaga catctaaagg cattcatttt
     1441 cgactatatt cttttttaca aaaaatataa caaccagata ttttaagctg atcctagatg
     1501 cacaaaaaat aaataaaagt ataaacctac ttcgtaggat acttcggggt actttttgtt
     1561 cggggttaga tgagcataac gcttgtagtt gatatttgag atcccctatc attgcagggt
     1621 gacagcggag cggcttcgca gagctgcatt aaccagggct tcgggcaggc caaaaactac
     1681 ggcacgctcc ggccacccag tccgccggag gactccggtt cagggagcgg ccaactagcc
     1741 gagaacctca cctatgcctg gcacaatatg gacatctttg gggcggtcaa tcagccgggc
     1801 tccggatggc ggcagctggt caaccggaca cgcggactat tctgcaacga gcgacacata
     1861 ccggcgccca ggaaacattt gctcaagaac ggtgagtttc tattcgcagt cggctgatct
     1921 gtgtgaaatc ttaataaagg gtccaattac caatttgaaa ctcagtttgc ggcgtggcct
     1981 atccgggcga acttttggcc gtgatgggca gttccggtgc cggaaagacg accctgctga
     2041 atgcccttgc ctttcgatcg ccgcagggca tccaagtatc gccatccggg atgcgactgc
     2101 tcaatggcca acctgtggac gccaaggaga tgcaggccag gtgcgcctat gtccagcagg
     2161 atgacctctt tatcggctcc ctaacggcca gggaacacct gattttccaa gccatggtgc
     2221 ggatgccacg acatctgacc tatcggcagc gagtggcccg cgtggatcag gtgatccagg
     2281 agctttcgct cagcaaatgt cagcacacga tcatcggtgt gcccggcagg gtgaaaggtc
     2341 tgtccggcgg agaaaggaag cgtctggcat tcgcctccga ggctctaacc gatccgccgc
     2401 ttctgatctg cgatgagccc acctccggac tggactcctt taccgcccac agcgtcgtcc
     2461 aggtgctgaa gaagctgtcg cagaagggca agaccgtcat cctgaccatt catcagccgt
     2521 cttccgagct gtttgagctc tttgacaaga tccttctgat ggccgagggc agggtagctt
     2581 tcttgggcac tcccagcgaa gccgtcgact tcttttccta gtgagttcga tgtgtttatt
     2641 aagggtatct agtattacat aacatctcaa ctcctatcca gcgtgggtgc ccagtgtcct
     2701 accaactaca atccggcgga cttttacgta caggtgttgg ccgttgtgcc cggacgggag
     2761 atcgagtccc gtgatcggat cgccaagata tgcgacaatt ttgccattag caaagtagcc
     2821 cgggatatgg agcagttgtt ggccaccaaa aatctggaga agccactgga gcagccggag
     2881 aatgggtaca cctacaaggc cacctggttc atgcagttcc gggcggtcct gtggcgatcc
     2941 tggctgtcgg tgctcaagga accactcctc gtaaaagtgc gacttattca gacaacggtg
     3001 agtggttcca gtggaaacaa atgatataac gcttacaatt cttggaaaca aattcgctag
     3061 attttagata gaattgcctg attccacacc cttcttagtt tttttcaatg agatgtatag
     3121 tttatagttt tgcagaagat aaataaattt catttaactc gcgaatatta atgagatgcg
     3181 agtaacattt taatttgcag atggttgcca tcttgattgg cctcatcttt ttgggccaac
     3241 aactcacgca agtgggtgtg atgaatatca acggagccat cttcctcttc ctgaccaaca
     3301 tgacctttca aaacgtcttt gccacgataa atgtaagtca tgtttagaat acatttgcat
     3361 tccaataatt tactaacttt ctaatgaatc gattcgattt aggtgttcac ctcagagctg
     3421 ccagttttta tgagggaggc ccgaagtcga ctttatcgct gtgacacata ctttctgggc
     3481 aaaacgattg ccgaattgcc gctttttctc acagtgccac tggtcttcac ggcgattgcc
     3541 tatccgatga tcggactgcg ggccggagtg ctgcacttct tcaactgcct ggcgctggtc
     3601 actctggtgg ccaatgtgtc aacgtccttc ggatatctaa tatcctgcgc cagctcctcg
     3661 acctcgatgg cgctgtctgt gggtccgccg gttatcatac cattcctgct ctttggcggc
     3721 ttcttcttga actcgggctc ggtgccagta tacctcaaat ggttgtcgta cctctcatgg
     3781 ttccgttacg ccaacgaggg tctgctgatt aaccaatggg cggacgtgga gccgggcgaa
     3841 attagctgca catcgtcgaa caccacgtgc cccagttcgg gcaaggtcat cctggagacg
     3901 cttaacttct ccgccgccga tctgccgctg gactacgtgg gtctggccat tctcatcgtg
     3961 agcttccggg tgctcgcata tctggctcta agacttcggg cccgacgcaa ggagtagccg
     4021 acatatatcc gaaataactg cttgtttttt ttttttacca ttattaccat cgtgtttact
     4081 gtttattgcc ccctcaaaaa gctaatgtaa ttatatttgt gccaataaaa acaagatatg
     4141 acctatagaa tacaagtatt tccccttcga acatccccac aagtagactt tggatttgtc
     4201 ttctaaccaa aagacttaca cacctgcata ccttacatca aaaactcgtt tatcgctaca
     4261 taaaacaccg ggatatattt tttatataca tacttttcaa atcgcgcgcc ctcttcataa
     4321 ttcacctcca ccacaccacg tttcgtagtt gctctttcgc tgtctcccac ccgctctccg
     4381 caacacattc accttttgtt cgacgacctt ggagcgactg tcgttagttc cgcgcgattc
     4441 ggttcgctca aatggttccg agtggttcat ttcgtctcaa tagaaattag taataaatat
     4501 ttgtatgtac aatttatttg ctccaatata tttgtatata tttccctcac agctatattt
     4561 attctaattt aatattatga ctttttaagg taattttttg tgacctgttc ggagtgatta
     4621 gcgttacaat ttgaactgaa agtgacatcc agtgtttgtt ccttgtgtag atgcatctca
     4681 aaaaaatggt gggcataata gtgttgttta tatatatcaa aaataacaac tataataata
     4741 agaatacatt taatttagaa aatgcttgga tttcactgga actaggctag cataacttcg
     4801 tataatgtat gctatacgaa gttatgctag cggatcctgg ccacgtaata agtgtgcgtt
     4861 gaatttattc gcaaaaacat tgcatatttt cggcaaagta aaattttgtt gcatacctta
     4921 tcaaaaaata agtgctgcat actttttaga gaaaccaaat aattttttat tgcatacccg
     4981 tttttaataa aatacattgc ataccctctt ttaataaaaa atattgcata ctttgacgaa
     5041 acaaattttc gttgcatacc caataaaaga ttattatatt gcatacccgt ttttaataaa
     5101 atacattgca taccctcttt taataaaaaa tattgcatac gttgacgaaa caaattttcg
     5161 ttgcataccc aataaaagat tattatattg catacctttt cttgccatac catttagccg
     5221 atcaattgtg ctcggcaaca gtatatttgt ggtgtgccaa ccaacaacag atccaagctg
     5281 gccgcggctc gataagcttg catgcctgca ggtcggagta ctgtcctccg agcggagtac
     5341 tgtcctccga gcggagtact gtcctccgag cggagtactg tcctccgagc ggagtactgt
     5401 cctccgagcg gagactctag ccctagggca tgcctgcagg tcggagtact gtcctccgag
     5461 cggagtactg tcctccgagc ggagtactgt cctccgagcg gagtactgtc ctccgagcgg
     5521 agtactgtcc tccgagcgga gactctagcg ctagcgcatg cctgcaggtc ggagtactgt
     5581 cctccgagcg gagtactgtc ctccgagcgg agtactgtcc tccgagcgga gtactgtcct
     5641 ccgagcggag tactgtcctc cgagcggaga ctctagcgct agcgcatgcc tgcaggtcgg
     5701 agtactgtcc tccgagcgga gtactgtcct ccgagcggag tactgtcctc cgagcggagt
     5761 actgtcctcc gagcggagta ctgtcctccg agcggagact ctagcgacgt cgagactcta
     5821 gcgagctcgc ccggggatcg agcgcagcgg tataaaaggg cgcggggtgg ctgagagcat
     5881 cagttgtgaa tgaatgttcg agccgagcag acgtgccgct gccttcgtta atatcctttg
     5941 aataagccaa ctttgaatca caagacgcat accaaacgaa ttcgcagatc ttaattaacc
     6001 ggtaccacaa gtttgtacaa aaaagctgaa cgagaaacgt aaaatgatat aaatatcaat
     6061 atattaaatt agattttgca taaaaaacag actacataat actgtaaaac acaacatatc
     6121 cagtcactat ggcggccgca ttaggcaccc caggctttac actttatgct tccggctcgt
     6181 ataatgtgtg gattttgagt taggatccgt cgagattttc aggagctaag gaagctaaaa
     6241 tggagaaaaa aatcactgga tataccaccg ttgatatatc ccaatggcat cgtaaagaac
     6301 attttgaggc atttcagtca gttgctcaat gtacctataa ccagaccgtt cagctggata
     6361 ttacggcctt tttaaagacc gtaaagaaaa ataagcacaa gttttatccg gcctttattc
     6421 acattcttgc ccgcctgatg aatgctcatc cggaattccg tatggcaatg aaagacggtg
     6481 agctggtgat atgggatagt gttcaccctt gttacaccgt tttccatgag caaactgaaa
     6541 cgttttcatc gctctggagt gaataccacg acgatttccg gcagtttcta cacatatatt
     6601 cgcaagatgt ggcgtgttac ggtgaaaacc tggcctattt ccctaaaggg tttattgaga
     6661 atatgttttt cgtctcagcc aatccctggg tgagtttcac cagttttgat ttaaacgtgg
     6721 ccaatatgga caacttcttc gcccccgttt tcaccatggg caaatattat acgcaaggcg
     6781 acaaggtgct gatgccgctg gcgattcagg ttcatcatgc cgtttgtgat ggcttccatg
     6841 tcggcagaat gcttaatgaa ttacaacagt actgcgatga gtggcagggc ggggcgtaaa
     6901 cgcgtggatc cggcttacta aaagccagat aacagtatgc gtatttgcgc gctgattttt
     6961 gcggtataag aatatatact gatatgtata cccgaagtat gtcaaaaaga ggtatgctat
     7021 gaagcagcgt attacagtga cagttgacag cgacagctat cagttgctca aggcatatat
     7081 gatgtcaata tctccggtct ggtaagcaca accatgcaga atgaagcccg tcgtctgcgt
     7141 gccgaacgct ggaaagcgga aaatcaggaa gggatggctg aggtcgcccg gtttattgaa
     7201 atgaacggct cttttgctga cgagaacagg ggctggtgaa atgcagttta aggtttacac
     7261 ctataaaaga gagagccgtt atcgtctgtt tgtggatgta cagagtgata ttattgacac
     7321 gcccgggcga cggatggtga tccccctggc cagtgcacgt ctgctgtcag ataaagtctc
     7381 ccgtgaactt tacccggtgg tgcatatcgg ggatgaaagc tggcgcatga tgaccaccga
     7441 tatggccagt gtgccggtct ccgttatcgg ggaagaagtg gctgatctca gccaccgcga
     7501 aaatgacatc aaaaacgcca ttaacctgat gttctgggga atataaatgt caggctccct
     7561 tatacacagc cagtctgcag gtcgaccata gtgactggat atgttgtgtt ttacagtatt
     7621 atgtagtctg ttttttatgc aaaatctaat ttaatatatt gatatttata tcattttacg
     7681 tttctcgttc agctttcttg tacaaagtgg tgatatcgca tgcggccgcg gcgcgcctct
     7741 agaaatgaat cgtttttaaa ataacaaatc aattgtttta taatattcgt acgattcttt
     7801 gattatgtaa taaaatgtga tcattaggaa gattacgaaa aatataaaaa atatgagttc
     7861 tgtgtgtata acaaatgctg taaacgccac aattgtgttt gttgcaaata aacccatgat
     7921 tatttgatta aaattgttgt tttctttgtt catagacaat agtgtgtttt gcctaaacgt
     7981 gtactgcata aactccatgc gagtgtatag cgagctagtg gctaacgctt gccccaccaa
     8041 agtagattcg tcaaaatcct caatttcatc accctcctcc aagtttaaca tttggccgtc
     8101 ggaattaact tctaaagatg ccacataatc taataaatga aatagagatt caaacgtggc
     8161 gtcatcgtcc gtttcgacca tttccgaaaa gaactcgggc ataaactcta tgatttctct
     8221 ggacgtggtg ttgtcgaaac tctcaaagta cgcagtcagg aacgtgcgcg acatgtcgtc
     8281 gggaaactcg cgcggaaaca tgttgttgta accgaacggg tcccatagcg ccaaaaccaa
     8341 atctgccagc gtcaatagaa tgagcacgat gccgacaatg gagctggctt ggatagcgat
     8401 tcgagttaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
     8461 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
     8521 tgtatcttat catgtctgga tcttggccac gtaataagtg tgcgttgaat ttattcgcaa
     8581 aaacattgca tattttcggc aaagtaaaat tttgttgcat accttatcaa aaaataagtg
     8641 ctgcatactt tttagagaaa ccaaataatt ttttattgca tacccgtttt taataaaata
     8701 cattgcatac cctcttttaa taaaaaatat tgcatacttt gacgaaacaa attttcgttg
     8761 catacccaat aaaagattat tatattgcat acccgttttt aataaaatac attgcatacc
     8821 ctcttttaat aaaaaatatt gcatacgttg acgaaacaaa ttttcgttgc atacccaata
     8881 aaagattatt atattgcata ccttttcttg ccataccatt tagccgatca attgtgctcg
     8941 gcaacagtat atttgtggtg tgccaaccaa caacggatcc actagtgtcg acgatgtagg
     9001 tcacggtctc gaagccgcgg tgcgggtgcc agggcgtgcc cttgggctcc ccgggcgcgt
     9061 actccacctc acccatctgg tccatcatga tgaacgggtc gaggtggcgg tagttgatcc
     9121 cggcgaacgc gcggcgcacc gggaagccct cgccctcgaa accgctgggc gcggtggtca
     9181 cggtgagcac gggacgtgcg acggcgtcgg cgggtgcgga tacgcggggc agcgtcagcg
     9241 ggttctcgac ggtcacggcg ggcatgtcga cactagttct agccagcttt tgttcccttt
     9301 agtgagggtt aatttcgagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt
     9361 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg
     9421 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt
     9481 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt
     9541 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
     9601 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
     9661 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
     9721 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     9781 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     9841 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     9901 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
     9961 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
    10021 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
    10081 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
    10141 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc
    10201 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
    10261 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
    10321 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
    10381 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
    10441 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
    10501 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
    10561 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
    10621 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
    10681 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
    10741 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
    10801 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
    10861 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
    10921 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
    10981 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
    11041 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
    11101 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
    11161 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
    11221 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
    11281 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
    11341 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
    11401 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
    11461 gcacatttcc ccgaaaagtg ccac
//
