LOCUS       Exported               11967 bp DNA     circular SYN 01-JUL-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pBID2_Gateway>QF2
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11967)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 2, 2021 from SnapGene Viewer 5.3.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..11967
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     rep_origin      complement(135..441)
                     /direction=LEFT
                     /label=F1 ori
     misc_feature    667..4785
                     /label=Mini-White
     misc_feature    4838..5268
                     /label=Gypsy Ins.
                     /label=gypsy insulator
     misc_recomb     5291..6995
                     /label=Gateway Cassette
     promoter        7011..7165
                     /label=DSCP
     promoter        7184..7249
                     /label=MHC IV16 Intron
     misc_feature    7266..7286
                     /label=Syn21
     CDS             7287..8339
                     /label=QF2
     misc_feature    8348..9014
                     /label=p10 3'UTR
     misc_feature    9027..9457
                     /label=Gypsy Ins.
                     /label=gypsy insulator(1)
     misc_feature    9470..9754
                     /label=attB phiC31
     rep_origin      complement(10202..10884)
                     /direction=LEFT
                     /label=ColE1 origin
     CDS             complement(10982..11641)
                     /label=AmpR
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgaat tgtaatacga ctcactatag ggcgaattgg gtacgtaccg
      661 ggcccctagt atgtatgtaa gttaataaaa cccatttttg cggaaagtag ataaaaaaaa
      721 catttttttt ttttactgca ctggatatca ttgaacttat ctgatcagtt ttaaatttac
      781 ttcgatccaa gggtatttga tgtaccaggt tctttcgatt acctctcact caaaatgaca
      841 ttccactcaa agtcagcgct gtttgcctcc ttctctgtcc acagaaatat cgccgtctct
      901 ttcgccgctg cgtccgctat ctctttcgcc accgtttgta gcgttacgta gcgtcaatgt
      961 ccgccttcag ttgcattttg tcagcggttt cgtgacgaag ctccaagcgg tttacgccat
     1021 caattaaaca caaagtgctg tgccaaaact cctctcgctt cttatttttg tttgtttttt
     1081 gagtgattgg ggtggtgatt ggttttgggt gggtaagcag gggaaagtgt gaaaaatccc
     1141 ggcaatgggc caagaggatc aggagctatt aattcgcgga ggcagcaaac acccatctgc
     1201 cgagcatctg aacaatgtga gtagtacatg tgcatacatc ttaagttcac ttgatctata
     1261 ggaactgcga ttgcaacatc aaattgtctg cggcgtgaga actgcgaccc acaaaaatcc
     1321 caaaccgcaa ttgcacaaac aaatagtgac acgaaacaga ttattctggt agctgttctc
     1381 gctatataag acaatttttg agatcatatc atgatcaaga catctaaagg cattcatttt
     1441 cgactatatt cttttttaca aaaaatataa caaccagata ttttaagctg atcctagatg
     1501 cacaaaaaat aaataaaagt ataaacctac ttcgtaggat acttcggggt actttttgtt
     1561 cggggttaga tgagcataac gcttgtagtt gatatttgag atcccctatc attgcagggt
     1621 gacagcggag cggcttcgca gagctgcatt aaccagggct tcgggcaggc caaaaactac
     1681 ggcacgctcc ggccacccag tccgccggag gactccggtt cagggagcgg ccaactagcc
     1741 gagaacctca cctatgcctg gcacaatatg gacatctttg gggcggtcaa tcagccgggc
     1801 tccggatggc ggcagctggt caaccggaca cgcggactat tctgcaacga gcgacacata
     1861 ccggcgccca ggaaacattt gctcaagaac ggtgagtttc tattcgcagt cggctgatct
     1921 gtgtgaaatc ttaataaagg gtccaattac caatttgaaa ctcagtttgc ggcgtggcct
     1981 atccgggcga acttttggcc gtgatgggca gttccggtgc cggaaagacg accctgctga
     2041 atgcccttgc ctttcgatcg ccgcagggca tccaagtatc gccatccggg atgcgactgc
     2101 tcaatggcca acctgtggac gccaaggaga tgcaggccag gtgcgcctat gtccagcagg
     2161 atgacctctt tatcggctcc ctaacggcca gggaacacct gattttccaa gccatggtgc
     2221 ggatgccacg acatctgacc tatcggcagc gagtggcccg cgtggatcag gtgatccagg
     2281 agctttcgct cagcaaatgt cagcacacga tcatcggtgt gcccggcagg gtgaaaggtc
     2341 tgtccggcgg agaaaggaag cgtctggcat tcgcctccga ggctctaacc gatccgccgc
     2401 ttctgatctg cgatgagccc acctccggac tggactcctt taccgcccac agcgtcgtcc
     2461 aggtgctgaa gaagctgtcg cagaagggca agaccgtcat cctgaccatt catcagccgt
     2521 cttccgagct gtttgagctc tttgacaaga tccttctgat ggccgagggc agggtagctt
     2581 tcttgggcac tcccagcgaa gccgtcgact tcttttccta gtgagttcga tgtgtttatt
     2641 aagggtatct agtattacat aacatctcaa ctcctatcca gcgtgggtgc ccagtgtcct
     2701 accaactaca atccggcgga cttttacgta caggtgttgg ccgttgtgcc cggacgggag
     2761 atcgagtccc gtgatcggat cgccaagata tgcgacaatt ttgccattag caaagtagcc
     2821 cgggatatgg agcagttgtt ggccaccaaa aatctggaga agccactgga gcagccggag
     2881 aatgggtaca cctacaaggc cacctggttc atgcagttcc gggcggtcct gtggcgatcc
     2941 tggctgtcgg tgctcaagga accactcctc gtaaaagtgc gacttattca gacaacggtg
     3001 agtggttcca gtggaaacaa atgatataac gcttacaatt cttggaaaca aattcgctag
     3061 attttagata gaattgcctg attccacacc cttcttagtt tttttcaatg agatgtatag
     3121 tttatagttt tgcagaagat aaataaattt catttaactc gcgaatatta atgagatgcg
     3181 agtaacattt taatttgcag atggttgcca tcttgattgg cctcatcttt ttgggccaac
     3241 aactcacgca agtgggtgtg atgaatatca acggagccat cttcctcttc ctgaccaaca
     3301 tgacctttca aaacgtcttt gccacgataa atgtaagtca tgtttagaat acatttgcat
     3361 ttcaataatt tactaacttt ctaatgaatc gattcgattt aggtgttcac ctcagagctg
     3421 ccagttttta tgagggaggc ccgaagtcga ctttatcgct gtgacacata ctttctgggc
     3481 aaaacgattg ccgaattgcc gctttttctc acagtgccac tggtcttcac ggcgattgcc
     3541 tatccgatga tcggactgcg ggccggagtg ctgcacttct tcaactgcct ggcgctggtc
     3601 actctggtgg ccaatgtgtc aacgtccttc ggatatctaa tatcctgcgc cagctcctcg
     3661 acctcgatgg cgctgtctgt gggtccgccg gttatcatac cattcctgct ctttggcggc
     3721 ttcttcttga actcgggctc ggtgccagta tacctcaaat ggttgtcgta cctctcatgg
     3781 ttccgttacg ccaacgaggg tctgctgatt aaccaatggg cggacgtgga gccgggcgaa
     3841 attagctgca catcgtcgaa caccacgtgc cccagttcgg gcaaggtcat cctggagacg
     3901 cttaacttct ccgccgccga tctgccgctg gactacgtgg gtctggccat tctcatcgtg
     3961 agcttccggg tgctcgcata tctggctcta agacttcggg cccgacgcaa ggagtagccg
     4021 acatatatcc gaaataactg cttgtttttt ttttttacca ttattaccat cgtgtttact
     4081 gtttattgcc ccctcaaaaa gctaatgtaa ttatatttgt gccaataaaa acaagatatg
     4141 acctatagaa tacaagtatt tccccttcga acatccccac aagtagactt tggatttgtc
     4201 ttctaaccaa aagacttaca cacctgcata ccttacatca aaaactcgtt tatcgctaca
     4261 taaaacaccg ggatatattt tttatataca tacttttcaa atcgcgcgcc ctcttcataa
     4321 ttcacctcca ccacaccacg tttcgtagtt gctctttcgc tgtctcccac ccgctctccg
     4381 caacacattc accttttgtt cgacgacctt ggagcgactg tcgttagttc cgcgcgattc
     4441 ggttcgctca aatggttccg agtggttcat ttcgtctcaa tagaaattag taataaatat
     4501 ttgtatgtac aatttatttg ctccaatata tttgtatata tttccctcac agctatattt
     4561 attctaattt aatattatga ctttttaagg taattttttg tgacctgttc ggagtgatta
     4621 gcgttacaat ttgaactgaa agtgacatcc agtgtttgtt ccttgtgtag atgcatctca
     4681 aaaaaatggt gggcataata gtgttgttta tatatatcaa aaataacaac tataataata
     4741 agaatacatt taatttagaa aatgcttgga tttcactgga actaggctag cataacttcg
     4801 tataatgtat gctatacgaa gttatgctag cggatcctgg ccacgtaata agtgtgcgtt
     4861 gaatttattc gcaaaaacat tgcatatttt cggcaaagta aaattttgtt gcatacctta
     4921 tcaaaaaata agtgctgcat actttttaga gaaaccaaat aattttttat tgcatacccg
     4981 tttttaataa aatacattgc ataccctctt ttaataaaaa atattgcata ctttgacgaa
     5041 acaaattttc gttgcatacc caataaaaga ttattatatt gcatacccgt ttttaataaa
     5101 atacattgca taccctcttt taataaaaaa tattgcatac gttgacgaaa caaattttcg
     5161 ttgcataccc aataaaagat tattatattg catacctttt cttgccatac catttagccg
     5221 atcaattgtg ctcggcaaca gtatatttgt ggtgtgccaa ccaacaacag atctgaattc
     5281 gcttaattaa acaagtttgt acaaaaaagc tgaacgagaa acgtaaaatg atataaatat
     5341 caatatatta aattagattt tgcataaaaa acagactaca taatactgta aaacacaaca
     5401 tatccagtca ctatggcggc cgcattaggc accccaggct ttacacttta tgcttccggc
     5461 tcgtataatg tgtggatttt gagttaggat ccgtcgagat tttcaggagc taaggaagct
     5521 aaaatggaga aaaaaatcac tggatatacc accgttgata tatcccaatg gcatcgtaaa
     5581 gaacattttg aggcatttca gtcagttgct caatgtacct ataaccagac cgttcagctg
     5641 gatattacgg cctttttaaa gaccgtaaag aaaaataagc acaagtttta tccggccttt
     5701 attcacattc ttgcccgcct gatgaatgct catccggaat tccgtatggc aatgaaagac
     5761 ggtgagctgg tgatatggga tagtgttcac ccttgttaca ccgttttcca tgagcaaact
     5821 gaaacgtttt catcgctctg gagtgaatac cacgacgatt tccggcagtt tctacacata
     5881 tattcgcaag atgtggcgtg ttacggtgaa aacctggcct atttccctaa agggtttatt
     5941 gagaatatgt ttttcgtctc agccaatccc tgggtgagtt tcaccagttt tgatttaaac
     6001 gtggccaata tggacaactt cttcgccccc gttttcacca tgggcaaata ttatacgcaa
     6061 ggcgacaagg tgctgatgcc gctggcgatt caggttcatc atgccgtttg tgatggcttc
     6121 catgtcggca gaatgcttaa tgaattacaa cagtactgcg atgagtggca gggcggggcg
     6181 taaacgcgtg gatccggctt actaaaagcc agataacagt atgcgtattt gcgcgctgat
     6241 ttttgcggta taagaatata tactgatatg tatacccgaa gtatgtcaaa aagaggtatg
     6301 ctatgaagca gcgtattaca gtgacagttg acagcgacag ctatcagttg ctcaaggcat
     6361 atatgatgtc aatatctccg gtctggtaag cacaaccatg cagaatgaag cccgtcgtct
     6421 gcgtgccgaa cgctggaaag cggaaaatca ggaagggatg gctgaggtcg cccggtttat
     6481 tgaaatgaac ggctcttttg ctgacgagaa caggggctgg tgaaatgcag tttaaggttt
     6541 acacctataa aagagagagc cgttatcgtc tgtttgtgga tgtacagagt gatattattg
     6601 acacgcccgg gcgacggatg gtgatccccc tggccagtgc acgtctgctg tcagataaag
     6661 tctcccgtga actttacccg gtggtgcata tcggggatga aagctggcgc atgatgacca
     6721 ccgatatggc cagtgtgccg gtctccgtta tcggggaaga agtggctgat ctcagccacc
     6781 gcgaaaatga catcaaaaac gccattaacc tgatgttctg gggaatataa atgtcaggct
     6841 cccttataca cagccagtct gcaggtcgac catagtgact ggatatgttg tgttttacag
     6901 tattatgtag tctgtttttt atgcaaaatc taatttaata tattgatatt tatatcattt
     6961 tacgtttctc gttcagcttt cttgtacaaa gtggtgataa acggccggcc gagctcgccc
     7021 ggggatcgag cgcagcggta taaaagggcg cggggtggct gagagcatca gttgtgaatg
     7081 aatgttcgag ccgagcagac gtgccgctgc cttcgttaat atcctttgaa taagccaact
     7141 ttgaatcaca agacgcatac caaacggtac cagatctaaa aggtaggttc aaccactgat
     7201 gcctaggcac accgaaacga ctaaccctaa ttcttatcct ttacttcagg cggccgcgtt
     7261 taaacaactt aaaaaaaaaa atcaaaatgc cacccaagcg caaaacgctt aacgctgcgg
     7321 ctgaggctaa cgctcatgcc gacggacacg ccgacggaaa cgccgacgga cacgtggcca
     7381 atacggccgc gtcctcgaat aatgcgaggt tcgctgatct cactaacatc gatactccgg
     7441 gtctgggacc cacaactacg accctgctcg tggaaccagc acgctcaaag cgtcaacgag
     7501 tgtcccgcgc atgcgaccag tgccgtgcag cccgagagaa atgcgacgga atacagcctg
     7561 cgtgtttccc gtgcgtttcc cagggaaggt cctgcactta tcaggcttcg ccgaaaaaga
     7621 ggggagttca aaccggttat attcgtacgc tggagctcgc cctcgcctgg atgtttgaaa
     7681 atgtcgcgcg ttccgaagat gccttgcata acctcctcgt ccgtgacgcc ggacaaggat
     7741 cagctctgct cgttggtaaa gattcgccgg ctgccgagcg actccatgcc cgttgggcta
     7801 ctagccgtgt caataagagc attacccgcc tcctccgtca gttggagctc cctcctaccg
     7861 ccacggctac ggcctcgata atgccgcacg tgatggagca gcctctcagt accagcatta
     7921 accccgtcaa cgaccgcttc aacggtattc ccaaccccac tccgtataac tccgatgcag
     7981 ctctcgatgc tatcactcag accaacgatt atggaagcgt aaatacacat ggtatcctct
     8041 ctacttaccc gccaccggct acgcacctta atgaagcttc cgtcgctctc gctcccggtg
     8101 gcgccccccc ccgaccgcct cctccgtatg ttgacagcac gaccaatcac ccgccgtacc
     8161 actcgaatct ggttccaatg gcgaactttg gttactcgac cgttgattac gatgccatgg
     8221 ttgacgattt ggctagcatt gaatacacgg acgctgtgga tgtcgaccca cagtttatga
     8281 ccaatctggg attcgttcct ggatgtaact tctccgacat taatacatac gaacagtgac
     8341 ctgcaggaat gaatcgtttt taaaataaca aatcaattgt tttataatat tcgtacgatt
     8401 ctttgattat gtaataaaat gtgatcatta ggaagattac gaaaaatata aaaaatatga
     8461 gttctgtgtg tataacaaat gctgtaaacg ccacaattgt gtttgttgca aataaaccca
     8521 tgattatttg attaaaattg ttgttttctt tgttcataga caatagtgtg ttttgcctaa
     8581 acgtgtactg cataaactcc atgcgagtgt atagcgagct agtggctaac gcttgcccca
     8641 ccaaagtaga ttcgtcaaaa tcctcaattt catcaccctc ctccaagttt aacatttggc
     8701 cgtcggaatt aacttctaaa gatgccacat aatctaataa atgaaataga gattcaaacg
     8761 tggcgtcatc gtccgtttcg accatttccg aaaagaactc gggcataaac tctatgattt
     8821 ctctggacgt ggtgttgtcg aaactctcaa agtacgcagt caggaacgtg cgcgacatgt
     8881 cgtcgggaaa ctcgcgcgga aacatgttgt tgtaaccgaa cgggtcccat agcgccaaaa
     8941 ccaaatctgc cagcgtcaat agaatgagca cgatgccgac aatggagctg gcttggatag
     9001 cgattcgagt taactctaga agatcttggc cacgtaataa gtgtgcgttg aatttattcg
     9061 caaaaacatt gcatattttc ggcaaagtaa aattttgttg cataccttat caaaaaataa
     9121 gtgctgcata ctttttagag aaaccaaata attttttatt gcatacccgt ttttaataaa
     9181 atacattgca taccctcttt taataaaaaa tattgcatac tttgacgaaa caaattttcg
     9241 ttgcataccc aataaaagat tattatattg catacccgtt tttaataaaa tacattgcat
     9301 accctctttt aataaaaaat attgcatacg ttgacgaaac aaattttcgt tgcataccca
     9361 ataaaagatt attatattgc ataccttttc ttgccatacc atttagccga tcaattgtgc
     9421 tcggcaacag tatatttgtg gtgtgccaac caacaacgga tccactagtg tcgacgatgt
     9481 aggtcacggt ctcgaagccg cggtgcgggt gccagggcgt gcccttgggc tccccgggcg
     9541 cgtactccac ctcacccatc tggtccatca tgatgaacgg gtcgaggtgg cggtagttga
     9601 tcccggcgaa cgcgcggcgc accgggaagc cctcgccctc gaaaccgctg ggcgcggtgg
     9661 tcacggtgag cacgggacgt gcgacggcgt cggcgggtgc ggatacgcgg ggcagcgtca
     9721 gcgggttctc gacggtcacg gcgggcatgt cgacactagt tctagccagc ttttgttccc
     9781 tttagtgagg gttaatttcg agcttggcgt aatcatggtc atagctgttt cctgtgtgaa
     9841 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct
     9901 ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc
     9961 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg
    10021 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
    10081 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
    10141 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
    10201 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
    10261 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
    10321 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
    10381 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
    10441 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
    10501 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
    10561 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
    10621 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg
    10681 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
    10741 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
    10801 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
    10861 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
    10921 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
    10981 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
    11041 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
    11101 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
    11161 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
    11221 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
    11281 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
    11341 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
    11401 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
    11461 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
    11521 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
    11581 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
    11641 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
    11701 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
    11761 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
    11821 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
    11881 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
    11941 cgcgcacatt tccccgaaaa gtgccac
//
