LOCUS       pJRK1_Prps-0            3062 bp ds-DNA     circular     27-MAY-2021
DEFINITION  .
FEATURES             Location/Qualifiers
     CDS             327..1142
                     /label="KanR"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     primer          1361..1377
                     /label="M13 F standard primer"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     cut site        1431..1437
                     /label="SapI"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     promoter        1442..1804
                     /label="Prps-0"
                     /ApEinfo_revcolor=#339933
                     /ApEinfo_fwdcolor=#339933
     CDS             1805..1807
                     /label="Translation 1805-1807"
     cut site        complement(1809..1815)
                     /label="SapI"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    complement(1846..1869)
                     /label="pUC M13 R"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     protein_bind    1857..1873
                     /label="lac operator"
                     /ApEinfo_revcolor=#31849b
                     /ApEinfo_fwdcolor=#31849b
     promoter        complement(1881..1887)
                     /label="lac promoter"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     promoter        complement(1888..1905)
                     /label="lac promoter"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     promoter        complement(1906..1911)
                     /label="lac promoter"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     rep_origin      complement(2419..3007)
                     /label="ori"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
ORIGIN
        1 GAGGTCTGCC TCGTGAAGAA GGTGTTGCTG ACTCATACCA GGCCTGAATC GCCCCATCAT
       61 CCAGCCAGAA AGTGAGGGAG CCACGGTTGA TGAGAGCTTT GTTGTAGGTG GACCAGTTGG
      121 TGATTTTGAA CTTTTGCTTT GCCACGGAAC GGTCTGCGTT GTCGGGAAGA TGCGTGATCT
      181 GATCCTTCAA CTCAGCAAAA GTTCGATTTA TTCAACAAAG CCACGTTGTG TCTCAAAATC
      241 TCTGATGTTA CATTGCACAA GATAAAAATA TATCATCATG AACAATAAAA CTGTCTGCTT
      301 ACATAAACAG TAATACAAGG GGTGTTATGA GCCATATTCA ACGGGAAACG TCTTGCTCGA
      361 AGCCGCGATT AAATTCCAAC ATGGATGCTG ATTTATATGG GTATAAATGG GCTCGCGATA
      421 ATGTCGGGCA ATCAGGTGCG ACAATCTATC GATTGTATGG GAAGCCCGAT GCGCCAGAGT
      481 TGTTTCTGAA ACATGGCAAA GGTAGCGTTG CCAATGATGT TACAGATGAG ATGGTCAGAC
      541 TAAACTGGCT GACGGAATTT ATGCCTCTTC CGACCATCAA GCATTTTATC CGTACTCCTG
      601 ATGATGCATG GTTACTCACC ACTGCGATCC CCGGGAAAAC AGCATTCCAG GTATTAGAAG
      661 AATATCCTGA TTCAGGTGAA AATATTGTTG ATGCGCTGGC AGTGTTCCTG CGCCGGTTGC
      721 ATTCGATTCC TGTTTGTAAT TGTCCTTTTA ACAGCGATCG CGTATTTCGT CTCGCTCAGG
      781 CGCAATCACG AATGAATAAC GGTTTGGTTG ATGCGAGTGA TTTTGATGAC GAGCGTAATG
      841 GCTGGCCTGT TGAACAAGTC TGGAAAGAAA TGCATAAGCT TTTGCCATTC TCACCGGATT
      901 CAGTCGTCAC TCATGGTGAT TTCTCACTTG ATAACCTTAT TTTTGACGAG GGGAAATTAA
      961 TAGGTTGTAT TGATGTTGGA CGAGTCGGAA TCGCAGACCG ATACCAGGAT CTTGCCATCC
     1021 TATGGAACTG CCTCGGTGAG TTTTCTCCTT CATTACAGAA ACGGCTTTTT CAAAAATATG
     1081 GTATTGATAA TCCTGATATG AATAAATTGC AGTTTCATTT GATGCTCGAT GAGTTTTTCT
     1141 AATCAGAATT GGTTAATTGG TTGTAACACT GGCAGAGCAT TACGCTGACT TGACGGGACG
     1201 GCGGCTTTGT TGAATAAATC GCATTCGCCA TTCAGGCTGC GCAACTGTTG GGAAGGGCGA
     1261 TCGGTGCGGG CCTCTTCGCT ATTACGCCAG CTGGCGAAAG GGGGATGTGC TGCAAGGCGA
     1321 TTAAGTTGGG TAACGCCAGG GTTTTCCCAG TCACGACGTT GTAAAACGAC GGCCAGTGCC
     1381 AAGCTTGCAT GCCTGCAGGT CGACTCTAGA GGATCCCCGG GTACCGAGct GCTCTTCgTG
     1441 GATTTTTGCT TTCGTCGTAA ATCTACACAC GCGTCTCTTC CGTGCGAGAG TCCAAGCCAG
     1501 CAGCCAAATT CGTTGACTGA GTATTCAACG TTTATACGTT GTCGGCAACG AGAAATAGGA
     1561 AAATGCATCG GGAAATGTTC TTTTTTCGAT TTTTTCCAAG GTTTTGACAA ATTTTACCAC
     1621 GAATTTTGCT ATGTTTTCAA TTAAAAAATA TGTTATTCAA CTGTTTCTAT GAGGAAAATA
     1681 AGGCTTTGCA TGTAATTTTC TTATTCAGCA TAATTTTTAA TTAATTTGAA TTTTCTGTCC
     1741 TAACGTTTAT TTTGTTTTCT TGGTTATGAC TGATCTGAAA TTAATTTTTG AATTTTAAGG
     1801 TAATATGcGA AGAGCCTCGA ATTCGTAATC ATGTCATAGC TGTTTCCTGT GTGAAATTGT
     1861 TATCCGCTCA CAATTCCACA CAACATACGA GCCGGAAGCA TAAAGTGTAA AGCCTGGGGT
     1921 GCCTAATGAG TGAGCTAACT CACATTAATT GCGTTGCGCT CACTGCCCGC TTTCCAGTCG
     1981 GGAAACCTGT CGTGCCAGCT GCATTAATGA ATCGGCCAAC GCGCGGGGAG AGGCGGTTTG
     2041 CGTATTGGCG AACTTTTGCT GAGTTGAAGG ATCAGATCAC GCATCTTCCC GACAACGCAG
     2101 ACCGTTCCGT GGCAAAGCAA AAGTTCAAAA TCAGTAACCG TCAGTGCCGA TAAGTTCAAA
     2161 GTTAAACCTG GTGTTGATAC CAACATTGAA ACGCTGATCG AAAACGCGCT GAAAAACGCT
     2221 GCTGAATGTG CGAGCTTCTT CCGCTTCCTC GCTCACTGAC TCGCTGCGCT CGGTCGTTCG
     2281 GCTGCGGCGA GCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA CAGAATCAGG
     2341 GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA
     2401 GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG
     2461 ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC
     2521 TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC
     2581 CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCAATGCTCA CGCTGTAGGT ATCTCAGTTC
     2641 GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG
     2701 CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC
     2761 ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA
     2821 GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGG ACAGTATTTG GTATCTGCGC
     2881 TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC
     2941 CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG
     3001 ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGATCCGTC
     3061 GA
//