LOCUS       pJRK107_CIBN            3359 bp ds-DNA     linear       27-MAY-2021
DEFINITION  .
FEATURES             Location/Qualifiers
     CDS             327..1142
                     /label="KanR"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     primer          1361..1377
                     /label="M13 F standard primer"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    1431..1437
                     /label="SapI"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     misc_feature    1442..2098
                     /label="CIBN-3xFLAG-linker"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     CDS             1952..1960
                     /label="3XFLAG partA"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     CDS             1952..1960
                     /label="Translation 1952-1960"
     misc_feature    1961..2011
                     /label="syntron2"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     cds             2012..2068
                     /label="3XFLAG part B"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     CDS             2012..2068
                     /label="Translation 2012-2068"
     misc_feature    2069..2098
                     /label="Flex Linker"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     CDS             2069..2098
                     /label="Translation 2069-2098"
     misc_feature    complement(2103..2109)
                     /label="SapI"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     misc_feature    complement(2143..2166)
                     /label="pUC M13 R"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     protein_bind    2154..2170
                     /label="lac operator"
                     /ApEinfo_revcolor=#31849b
                     /ApEinfo_fwdcolor=#31849b
     promoter        complement(2178..2184)
                     /label="lac promoter"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     promoter        complement(2185..2202)
                     /label="lac promoter"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     promoter        complement(2203..2208)
                     /label="lac promoter"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     rep_origin      complement(2716..3304)
                     /label="ori"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
ORIGIN
        1 GAGGTCTGCC TCGTGAAGAA GGTGTTGCTG ACTCATACCA GGCCTGAATC GCCCCATCAT
       61 CCAGCCAGAA AGTGAGGGAG CCACGGTTGA TGAGAGCTTT GTTGTAGGTG GACCAGTTGG
      121 TGATTTTGAA CTTTTGCTTT GCCACGGAAC GGTCTGCGTT GTCGGGAAGA TGCGTGATCT
      181 GATCCTTCAA CTCAGCAAAA GTTCGATTTA TTCAACAAAG CCACGTTGTG TCTCAAAATC
      241 TCTGATGTTA CATTGCACAA GATAAAAATA TATCATCATG AACAATAAAA CTGTCTGCTT
      301 ACATAAACAG TAATACAAGG GGTGTTATGA GCCATATTCA ACGGGAAACG TCTTGCTCGA
      361 AGCCGCGATT AAATTCCAAC ATGGATGCTG ATTTATATGG GTATAAATGG GCTCGCGATA
      421 ATGTCGGGCA ATCAGGTGCG ACAATCTATC GATTGTATGG GAAGCCCGAT GCGCCAGAGT
      481 TGTTTCTGAA ACATGGCAAA GGTAGCGTTG CCAATGATGT TACAGATGAG ATGGTCAGAC
      541 TAAACTGGCT GACGGAATTT ATGCCTCTTC CGACCATCAA GCATTTTATC CGTACTCCTG
      601 ATGATGCATG GTTACTCACC ACTGCGATCC CCGGGAAAAC AGCATTCCAG GTATTAGAAG
      661 AATATCCTGA TTCAGGTGAA AATATTGTTG ATGCGCTGGC AGTGTTCCTG CGCCGGTTGC
      721 ATTCGATTCC TGTTTGTAAT TGTCCTTTTA ACAGCGATCG CGTATTTCGT CTCGCTCAGG
      781 CGCAATCACG AATGAATAAC GGTTTGGTTG ATGCGAGTGA TTTTGATGAC GAGCGTAATG
      841 GCTGGCCTGT TGAACAAGTC TGGAAAGAAA TGCATAAGCT TTTGCCATTC TCACCGGATT
      901 CAGTCGTCAC TCATGGTGAT TTCTCACTTG ATAACCTTAT TTTTGACGAG GGGAAATTAA
      961 TAGGTTGTAT TGATGTTGGA CGAGTCGGAA TCGCAGACCG ATACCAGGAT CTTGCCATCC
     1021 TATGGAACTG CCTCGGTGAG TTTTCTCCTT CATTACAGAA ACGGCTTTTT CAAAAATATG
     1081 GTATTGATAA TCCTGATATG AATAAATTGC AGTTTCATTT GATGCTCGAT GAGTTTTTCT
     1141 AATCAGAATT GGTTAATTGG TTGTAACACT GGCAGAGCAT TACGCTGACT TGACGGGACG
     1201 GCGGCTTTGT TGAATAAATC GCATTCGCCA TTCAGGCTGC GCAACTGTTG GGAAGGGCGA
     1261 TCGGTGCGGG CCTCTTCGCT ATTACGCCAG CTGGCGAAAG GGGGATGTGC TGCAAGGCGA
     1321 TTAAGTTGGG TAACGCCAGG GTTTTCCCAG TCACGACGTT GTAAAACGAC GGCCAGTGCC
     1381 AAGCTTGCAT GCCTGCAGGT CGACTCTAGA GGATCCCCGG GTACCGAGga GCTCTTCgAA
     1441 GATGAACGGA GCTATCGGAG GAGACCTTCT CCTCAACTTC CCAGACATGT CCGTCCTCGA
     1501 GCGTCAACGT GCTCACCTCA AGTACCTCAA CCCAACCTTC GATTCCCCAC TCGCCGGATT
     1561 CTTCGCCGAT TCTTCCATGA TCACCGGAGG AGAGATGGAC TCCTACCTTT CCACCGCCGG
     1621 ACTCAATCTT CCAATGATGT ACGGAGAGAC CACCGTCGAG GGAGATTCCC GTCTCTCCAT
     1681 CTCCCCAGAG ACCACCCTTG GAACTGGAAA CTTCAAGGCC GCCAAGTTCG ATACCGAGAC
     1741 TAAGGATTGC AACGAGGCCG CCAAGAAGAT GACCATGAAC CGTGATGACC TCGTCGAAGA
     1801 AGGAGAAGAG GAGAAGTCCA AGATCACCGA GCAAAACAAC GGATCCACCA AGTCCATCAA
     1861 GAAGATGAAG CACAAGGCCA AGAAGGAAGA GAACAACTTC TCTAACGATT CCTCCAAGGT
     1921 CACCAAGGAG CTCGAGAAGA CCGATTACAT CGATTACAAG gtaagtttaa acatatatat
     1981 actaactaac cctgattatt taaattttca gGATCACGAT GGAGACTACA AGGACCACGA
     2041 TATCGACTAC AAGGACGATG ACGATAAAGG ATCCGGAGGt GGAGGtGGcG GATCCGGgAC
     2101 GcGAAGAGCt taCTCGAATT CGTAATCATG TCATAGCTGT TTCCTGTGTG AAATTGTTAT
     2161 CCGCTCACAA TTCCACACAA CATACGAGCC GGAAGCATAA AGTGTAAAGC CTGGGGTGCC
     2221 TAATGAGTGA GCTAACTCAC ATTAATTGCG TTGCGCTCAC TGCCCGCTTT CCAGTCGGGA
     2281 AACCTGTCGT GCCAGCTGCA TTAATGAATC GGCCAACGCG CGGGGAGAGG CGGTTTGCGT
     2341 ATTGGCGAAC TTTTGCTGAG TTGAAGGATC AGATCACGCA TCTTCCCGAC AACGCAGACC
     2401 GTTCCGTGGC AAAGCAAAAG TTCAAAATCA GTAACCGTCA GTGCCGATAA GTTCAAAGTT
     2461 AAACCTGGTG TTGATACCAA CATTGAAACG CTGATCGAAA ACGCGCTGAA AAACGCTGCT
     2521 GAATGTGCGA GCTTCTTCCG CTTCCTCGCT CACTGACTCG CTGCGCTCGG TCGTTCGGCT
     2581 GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA
     2641 TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC
     2701 CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG
     2761 CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG
     2821 AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT
     2881 TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA ATGCTCACGC TGTAGGTATC TCAGTTCGGT
     2941 GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG
     3001 CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT
     3061 GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT
     3121 CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGGACA GTATTTGGTA TCTGCGCTCT
     3181 GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC
     3241 CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC
     3301 TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG ATCCGTCGA
//