LOCUS       pJRK143_Pelt-2          6346 bp ds-DNA     linear       27-MAY-2021
DEFINITION  .
FEATURES             Location/Qualifiers
     CDS             327..1142
                     /label="KanR"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     primer          1361..1377
                     /label="M13 F standard primer"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    1433..1439
                     /label="SapI"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     misc_feature    1441..1443
                     /label="TGG overhang"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     promoter        1444..5083
                     /label="Pelt-2"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    5084..5086
                     /label="ATG overhang"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    complement(5088..5094)
                     /label="SapI"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    complement(5130..5153)
                     /label="pUC M13 R"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     protein_bind    5141..5157
                     /label="lac operator"
                     /ApEinfo_revcolor=#31849b
                     /ApEinfo_fwdcolor=#31849b
     promoter        complement(5165..5171)
                     /label="lac promoter"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     promoter        complement(5172..5189)
                     /label="lac promoter"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     promoter        complement(5190..5195)
                     /label="lac promoter"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     rep_origin      complement(5703..6291)
                     /label="ori"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
ORIGIN
        1 GAGGTCTGCC TCGTGAAGAA GGTGTTGCTG ACTCATACCA GGCCTGAATC GCCCCATCAT
       61 CCAGCCAGAA AGTGAGGGAG CCACGGTTGA TGAGAGCTTT GTTGTAGGTG GACCAGTTGG
      121 TGATTTTGAA CTTTTGCTTT GCCACGGAAC GGTCTGCGTT GTCGGGAAGA TGCGTGATCT
      181 GATCCTTCAA CTCAGCAAAA GTTCGATTTA TTCAACAAAG CCACGTTGTG TCTCAAAATC
      241 TCTGATGTTA CATTGCACAA GATAAAAATA TATCATCATG AACAATAAAA CTGTCTGCTT
      301 ACATAAACAG TAATACAAGG GGTGTTATGA GCCATATTCA ACGGGAAACG TCTTGCTCGA
      361 AGCCGCGATT AAATTCCAAC ATGGATGCTG ATTTATATGG GTATAAATGG GCTCGCGATA
      421 ATGTCGGGCA ATCAGGTGCG ACAATCTATC GATTGTATGG GAAGCCCGAT GCGCCAGAGT
      481 TGTTTCTGAA ACATGGCAAA GGTAGCGTTG CCAATGATGT TACAGATGAG ATGGTCAGAC
      541 TAAACTGGCT GACGGAATTT ATGCCTCTTC CGACCATCAA GCATTTTATC CGTACTCCTG
      601 ATGATGCATG GTTACTCACC ACTGCGATCC CCGGGAAAAC AGCATTCCAG GTATTAGAAG
      661 AATATCCTGA TTCAGGTGAA AATATTGTTG ATGCGCTGGC AGTGTTCCTG CGCCGGTTGC
      721 ATTCGATTCC TGTTTGTAAT TGTCCTTTTA ACAGCGATCG CGTATTTCGT CTCGCTCAGG
      781 CGCAATCACG AATGAATAAC GGTTTGGTTG ATGCGAGTGA TTTTGATGAC GAGCGTAATG
      841 GCTGGCCTGT TGAACAAGTC TGGAAAGAAA TGCATAAGCT TTTGCCATTC TCACCGGATT
      901 CAGTCGTCAC TCATGGTGAT TTCTCACTTG ATAACCTTAT TTTTGACGAG GGGAAATTAA
      961 TAGGTTGTAT TGATGTTGGA CGAGTCGGAA TCGCAGACCG ATACCAGGAT CTTGCCATCC
     1021 TATGGAACTG CCTCGGTGAG TTTTCTCCTT CATTACAGAA ACGGCTTTTT CAAAAATATG
     1081 GTATTGATAA TCCTGATATG AATAAATTGC AGTTTCATTT GATGCTCGAT GAGTTTTTCT
     1141 AATCAGAATT GGTTAATTGG TTGTAACACT GGCAGAGCAT TACGCTGACT TGACGGGACG
     1201 GCGGCTTTGT TGAATAAATC GCATTCGCCA TTCAGGCTGC GCAACTGTTG GGAAGGGCGA
     1261 TCGGTGCGGG CCTCTTCGCT ATTACGCCAG CTGGCGAAAG GGGGATGTGC TGCAAGGCGA
     1321 TTAAGTTGGG TAACGCCAGG GTTTTCCCAG TCACGACGTT GTAAAACGAC GGCCAGTGCC
     1381 AAGCTTGCAT GCCTGCAGGT CGACTCTAGA GGATCCCCGG GTACCGAGAG CTGCTCTTCg
     1441 TGGTAATTTC GAAATGTATG AACTCCAATT CTTGATAACC AATTCCTGAC TCTGTAACTA
     1501 TTATTATTAT AATCCTATTA CCCACCTTTT TTCCCATTCT GCTCTTAGTT CTCCCCCAAC
     1561 CCGTACATTT TCTCAACTAA TAAAGACAAT GAGAAAGTGA GAGAGAATAA GACGAACTGA
     1621 AAAGAATGTG TAAACAGTTT ATTGCCTCTC GATTGTACCA TCTAGTGTCT TGTCGTGTGC
     1681 ATAACTACTG ATAACAGATC GAATGTGAAA GACCCAATAA ATGCTTGGAA AGTATCAAAT
     1741 ATGTTTAGAA ATAGTATTTA GAGACTTATG TGGGACGAAA AAACAGGAGC ATTAAGAATA
     1801 CAGTTGCAAA CATTCATCAA GTTATAATCT AATAATATAA ATTCAAAAAT GTTAATGATA
     1861 AAAAATGATG ATCAAAGTAG GAAATTAGTG GCGAGAAAAC AATAAATCAT CAGAAAATGA
     1921 CTGATCTTGA GTAGAGTGCT TTTTTTGTAG ATTTCTGATT CTAAAACTGA AAAAAAAACT
     1981 AAAAATGCTC GATGAGTCAT ACTAATTGAA AAATAATCAG ATTGAAAACG TTAAAAATGT
     2041 ACTTGCTAAC ATACTTCCAT GGTTATTTTT GAACACAAAT AAAGTTATAT ACATCTTTAC
     2101 CGGCACCAGA AGAAATCACC GCTAACCAAT GTTCAGTTTT TACCTGAATA TCCCTGAAAG
     2161 TACAAAAAAA TCAATGTTCA ATCAAGTTTT TGCATGCCTT CGTGTGATGG CTCGCACCAA
     2221 GTATTCTGAC AGTTATAAAA TGTTTCCTGT GATCATGAAT TTGAAAAAAA AAGAACTGAT
     2281 GGGAAAATGT GATATTCATA AATGAAATGT TTAATAAACA AAAAAGAACC GTCATCACAA
     2341 ATCTACTCTG AAACGTTCAC AGTTCCTAAT TGACCAATTC AGAATTTTTT GTACTGACTT
     2401 AATAAATATA AATTTACAGT TTTAAGAAAC ATAAATTGAA TTATATAAAT AAAAAAAAAA
     2461 AAACTAAAAT GAAGACGTGA TGGAATGTTT CACGTATTAA AAAAGCTCTC GGACCGTTTG
     2521 ACATCCAGAA ATTATGTGAA AGTGATTTTC TAACCCTTTG ACGTCAAAAA AGTTTTTCTT
     2581 TTTTGAAAGA TACGCTGTTT AGAAATACTT ATACAGCTGA TTTTAGAATC TCCAAAAAAC
     2641 CTTCAATTAC TTAACAAACT TTCTAAATAA AGAATTTTGA TTTTGATTTT CTTTGAACTT
     2701 CAAACCCCCC GTTAAAAAAC CAAATTAAAA AAAAGATACC AGATTAATTT TAAAGTGAAA
     2761 AATATAAATT TATTCCCGAC TGTTAAAAAC GGTTTATAAA CGGAGTCACA ACACTCATAT
     2821 ACAACTATCG ACACCAAATA AATAACTTCG AAAGCTAATT GTGTCATTTC GGATATAGAG
     2881 AGTAGTGTGA GCAGATGTGT TTTTGCAGAA CTAAATTGAT GACACTATTA TGGAATATAA
     2941 TGACCAAATG TTAAATGTGT TAAGGTTTGA TATCAAAACC TGTATTTTCT TTTTATACAG
     3001 AATTGATAAT GTTATCTTCA ATTGATTTCT ACTTCTGAGC TACGGCGATA CGAGGACGCA
     3061 TTCTCAACGA TAATGTTGCC ATTTTGTCCT GATAATTTTT TTACTGATTG TTTCAGAACA
     3121 CCCATAGTTT TTCTCTATTA AACGTTCATC CTTGACTTCC CCGAGTTTGC TGGCTGAATA
     3181 GGAAATTTGA AGACAAAAAG GAAAGAATCG GCTCAAACGT CATGCAACTG ATAAGGCGAC
     3241 CGTACTTCAT TTCAAAAGAA GCTCACTTAC TGAGCGCAAA CATTGAGAAA TGAGAGCAAA
     3301 AGAAGTGGTT TTACAACATG CTAATGTTTT TAGACCTTGT ACCCAATAAT ATTACTGTAG
     3361 TATACAGTTC GGAGAGCATA TGGTTGAAAT CTTGAAATAC CAATTTATCA CTAGTTTGAT
     3421 TGTGTTATCG ATGTATAAAG ATATATTTTA TCATTTTTGA TTATTATCTG ATATTGTGGG
     3481 GTGTGAAGTA ATATTATGTG CGTGTGTGGC TGATTATCGA AAAAAACTGA AAATTATCAA
     3541 TTTTTCTACA GGTTATCTTT TTTTGTTTTA TTTTTCATTA TTGTATTCTT CATACTCCTT
     3601 ATCCTGCCCG AATCACAAAA GTTCCAGACA ACTTGAAGGT CGCATCACGT TTTGTTTATA
     3661 AGAATGTTGA ACTGGTCAGC TTTGGAGACA GAACTGTCCT CCAAATGTTG AATATGTTTC
     3721 CGTAAAACAC ACTGTGTAAC AAAAAGCGTA CCCTTTGATC CGGTATTCTT GAAAAAAAAC
     3781 ATAGAAGGGC GTACTTTTCA TATCTCACAA ATATGCGTTT TTTATTTTAT AATAATATCA
     3841 TTTATTGATA ATTATATGTA TTTGTATGTT TTCTTGTCTT TAAAATTATG GTGTGACATC
     3901 ACAGTTTAAA AATTATATGA AAACTAATGA GTGAAAAGTT AAAAATGTAT AGTTTGCCGT
     3961 ATTTTCTCCA TTAGTATTGT ATTCAAAACT TATTTCCAAT TGGTGAGCAC TATAAACTTT
     4021 GTAGGGTAAT TGAGGTTCAA TACTAATTTT TGAATATCTA GCTTTTGACA AATTATTCAA
     4081 GTTTTGACAA TGATGCCTTA TCAAATTAAA AAAAATTACC ATAAATTCTT GAAAGCTTTT
     4141 GATTTTGTTT CACTCTGTGA AGGATAGTAA TTTATCAACA TTTTAACTAG ATACATCTGT
     4201 TCTGGCAATA AATTAAAGCA AGACTATTAC TATGTGCAGT ATTAATAGCT GCATTGCAAC
     4261 TTGTTTGAAA GTTTCAGTGA AAACGCAAAA ACTCAATTTA ATTTTCAAGT TTGTTTGCAA
     4321 AATTGGATAT ATGTATCCAT CAAAAGTCTG ATCGCCTGTT TTATCTACTT ATTGTCTCCT
     4381 CAGTTGATTT TTTTGTAGTT ATTATAAACG CTACTTTGAA ACCAATATTT TCTTCCTCAT
     4441 TCCACTTTTT TCAATTACCA ACTACCGTAC ATGCAATGAT GGGCGGACCC GAGTCCCATG
     4501 TTTGGCTGGA AGTGGGTGGT TGTCTGCGTA TATGAGCGAC AGAGGTCGGG GCTGAAACTG
     4561 ATAAGAATAG TCGACACTAA CGCCATAATC GCTAGCCAGC CATCATGCAC ACCGAGCTCG
     4621 GTGTGCACAC CATCTTTCTT TTCAAACCAA TACGCTTTGT GCCTTCATTG ACAATTTTCT
     4681 TTTTGATAAA ATCAGCCTAT CTATACTTCC CAATCATTTT TAGTCTTATC GTTGAACAGC
     4741 TATCGAGGTG CCACTGTTTT CACTGATATC TTCTAAGTTA CTATGGCATT AACATCTTTG
     4801 TGTTCTTATT TTATGGGTTA TTTTAATTAA TTTTTGCAGT TAATTTTTGG AATGAGCAAG
     4861 AAAATGTTAA TTGTAATATC TTCGTCTGAA AATTGTCTTC AAATAGTTTT AATTTTAAAG
     4921 GCAGTATTTA AGAAAATACA CTTCTCGAAG CATTTTTGAA TTTTTGAATT TTTAAACTGC
     4981 TTGATGTTTT AGGTGCCACT GTTTTCATTG ATAAGTTTTG ATGTATAAAT GCTTGATTTT
     5041 CTTGGCATTC TAATAAAATA GAAACTAGAA AATAGATTAT AGAAAGcGAA GAGCACttaC
     5101 TCGAATTCGT AATCATGTCA TAGCTGTTTC CTGTGTGAAA TTGTTATCCG CTCACAATTC
     5161 CACACAACAT ACGAGCCGGA AGCATAAAGT GTAAAGCCTG GGGTGCCTAA TGAGTGAGCT
     5221 AACTCACATT AATTGCGTTG CGCTCACTGC CCGCTTTCCA GTCGGGAAAC CTGTCGTGCC
     5281 AGCTGCATTA ATGAATCGGC CAACGCGCGG GGAGAGGCGG TTTGCGTATT GGCGAACTTT
     5341 TGCTGAGTTG AAGGATCAGA TCACGCATCT TCCCGACAAC GCAGACCGTT CCGTGGCAAA
     5401 GCAAAAGTTC AAAATCAGTA ACCGTCAGTG CCGATAAGTT CAAAGTTAAA CCTGGTGTTG
     5461 ATACCAACAT TGAAACGCTG ATCGAAAACG CGCTGAAAAA CGCTGCTGAA TGTGCGAGCT
     5521 TCTTCCGCTT CCTCGCTCAC TGACTCGCTG CGCTCGGTCG TTCGGCTGCG GCGAGCGGTA
     5581 TCAGCTCACT CAAAGGCGGT AATACGGTTA TCCACAGAAT CAGGGGATAA CGCAGGAAAG
     5641 AACATGTGAG CAAAAGGCCA GCAAAAGGCC AGGAACCGTA AAAAGGCCGC GTTGCTGGCG
     5701 TTTTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG
     5761 TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG
     5821 CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA
     5881 AGCGTGGCGC TTTCTCAATG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC
     5941 TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT
     6001 AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT
     6061 GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG
     6121 CCTAACTACG GCTACACTAG AAGGACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT
     6181 ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT
     6241 GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGATCCT
     6301 TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGATC CGTCGA
//