LOCUS       pJRK86                  4657 bp ds-DNA     circular     27-MAY-2021
DEFINITION  .
FEATURES             Location/Qualifiers
     primer_bind     306..325
                     /label="T3"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     promoter        385..747
                     /label="Prps-0"
                     /ApEinfo_revcolor=#339933
                     /ApEinfo_fwdcolor=#339933
     misc_feature    748..882
                     /label="AID"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     CDS             748..882
                     /label="Translation 748-882"
     CDS             883..1185
                     /label="Translation 883-1185"
     misc_feature    883..1743
                     /label="GFP with introns"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     CDS             1234..1308
                     /label="Translation 1234-1308"
     CDS             1360..1479
                     /label="Translation 1360-1479"
     CDS             1531..1749
                     /label="Translation 1531-1749"
     3'UTR           1750..2101
                     /label="unc-54 3'UTR"
                     /ApEinfo_revcolor=#990000
                     /ApEinfo_fwdcolor=#990000
     primer_bind     complement(2154..2174)
                     /label="T7"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     rep_origin      2358..2664
                     /label="F1 ori"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     CDS             3125..3784
                     /label="AmpR"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     rep_origin      3882..4564
                     /label="ColE1 origin"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
ORIGIN
        1 gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag
       61 gaagcggccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct
      121 ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt
      181 agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg
      241 gaattgtgag cggataacaa tttcacacag gaaacagcta tgaccatgat tacgccaagc
      301 gcgcaattaa ccctcactaa agggaacaaa agctggagct ccaccgcggt ggcggccgct
      361 ctagaagggc tgcaggaatt cgatATTTTT GCTTTCGTCG TAAATCTACA CACGCGTCTC
      421 TTCCGTGCGA GAGTCCAAGC CAGCAGCCAA ATTCGTTGAC TGAGTATTCA ACGTTTATAC
      481 GTTGTCGGCA ACGAGAAATA GGAAAATGCA TCGGGAAATG TTCTTTTTTC GATTTTTTCC
      541 AAGGTTTTGA CAAATTTTAC CACGAATTTT GCTATGTTTT CAATTAAAAA ATATGTTATT
      601 CAACTGTTTC TATGAGGAAA ATAAGGCTTT GCATGTAATT TTCTTATTCA GCATAATTTT
      661 TAATTAATTT GAATTTTCTG TCCTAACGTT TATTTTGTTT TCTTGGTTAT GACTGATCTG
      721 AAATTAATTT TTGAATTTTA AGGTAATATG CCTAAAGATC CAGCCAAACC TCCGGCCAAG
      781 GCACAAGTTG TGGGATGGCC ACCGGTGAGA TCATACCGGA AGAACGTGAT GGTTTCCTGC
      841 CAAAAATCAA GCGGTGGCCC GGAGGCGGCG GCGTTCGTGA AGATGAGTAA AGGAGAAGAA
      901 TTGTTCACTG GAGTTGTCCC AATCCTCGTC GAGCTCGACG GAGACGTCAA CGGACACAAG
      961 TTCTCCGTCT CCGGAGAGGG AGAGGGAGAC GCCACCTACG GAAAGCTCAC CCTCAAGTTC
     1021 ATCTGCACCA CCGGAAAGCT CCCAGTCCCA TGGCCAACCC TCGTCACCAC CTTCTGCTAC
     1081 GGAGTCCAAT GCTTCTCCCG TTACCCAGAC CACATGAAGC GTCACGACTT CTTCAAGTCC
     1141 GCCATGCCAG AGGGATACGT CCAAGAGCGT ACCATCTTCT TCAAGgtaag tttaaacata
     1201 tatatactaa ctactgatta tttaaatttt cagGACGACG GAAACTACAA GACCCGTGCC
     1261 GAGGTCAAGT TCGAGGGAGA CACCCTCGTC AACCGTATCG AGCTCAAGgt aagtttaaac
     1321 agttcggtac taactaacca tacatattta aattttcagG GAATCGACTT CAAGGAGGAC
     1381 GGAAACATCC TCGGACACAA GCTCGAGTAC AACTACAACT CCCACAACGT CTACATCATG
     1441 GCCGACAAGC AAAAGAACGG AATCAAGGTC AACTTCAAGg taagtttaaa catgatttta
     1501 ctaactaact aatctgattt aaattttcag ATCCGTCACA ACATCGAGGA CGGATCCGTC
     1561 CAACTCGCCG ACCACTACCA ACAAAACACC CCAATCGGAG ACGGACCAGT CCTCCTCCCA
     1621 GACAACCACT ACCTCTCCAC CCAATCCGCC CTCTCCAAGG ACCCAAACGA GAAGCGTGAC
     1681 CACATGGTCC TCCTCGAGTT CGTCACCGCC GCCGGAATCA CCCACGGAAT GGACGAGCTC
     1741 TACAcgTAAT CCAATTACTC TTCAACATCC CTACATGCTC TTTCTCCCTG TGCTCCCACC
     1801 CCCTATTTTT GTTATTATCA AAAAACTTCT CTTAATTTCT TTGTTTTTTA GCTTCTTTTA
     1861 AGTCACCTCT AACAATGAAA TTGTGTAGAT TCAAAAATAG AATTAATTCG TAATAAAAAG
     1921 TCGAAAAAAA TTGTGCTCCC TCCCCCCATT AATAATAATT CTATCCCAAA ATCTACACAA
     1981 TGTTCTGTGT ACACTTCTTA TGTTTTTTAC TTCTGATAAA TTTTTTTGAA ACATCATAGA
     2041 AAAAACCGCA CACAAAATAC CTTATCATAT GTTACGTTTC AGTTTATGAC CGCAATTTTT
     2101 Aatcaagctt atcgataccg tcgacctcga gggggggccc ggtacccaat tcgccctata
     2161 gtgagtcgta ttacgcgcgc tcactggccg tcgttttaca acgtcgtgac tgggaaaacc
     2221 ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata
     2281 gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatggg
     2341 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
     2401 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
     2461 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
     2521 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc
     2581 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
     2641 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat
     2701 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta
     2761 acgcgaattt taacaaaata ttaacgctta caatttaggt ggcacttttc ggggaaatgt
     2821 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
     2881 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca
     2941 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc
     3001 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat
     3061 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc
     3121 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg
     3181 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc
     3241 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat
     3301 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga
     3361 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc
     3421 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc
     3481 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt
     3541 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc
     3601 tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc
     3661 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca
     3721 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca
     3781 ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt
     3841 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
     3901 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     3961 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     4021 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     4081 cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa
     4141 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     4201 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     4261 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     4321 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     4381 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     4441 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     4501 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     4561 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt
     4621 atcccctgat tctgtggata accgtattac cgccttt
//