LOCUS       pJRK136                 4864 bp ds-DNA     linear       27-MAY-2021
DEFINITION  .
FEATURES             Location/Qualifiers
     primer_bind     306..325
                     /label="T3"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    385..747
                     /label="Prps-0"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    748..1404
                     /label="CIBN-3xFLAG-linker"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     CDS             1258..1266
                     /label="3XFLAG partA"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    1267..1317
                     /label="syntron2"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     cds             1318..1374
                     /label="3XFLAG part B"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     CDS             1318..1407
                     /label="Translation 1318-1407"
     misc_feature    1375..1404
                     /label="Flex Linker"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    1408..1950
                     /label="MP"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    1579..1629
                     /label="syntron2"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    1957..2308
                     /label="unc-54 3' UTR"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     primer_bind     complement(2361..2381)
                     /label="T7"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     rep_origin      2565..2871
                     /label="F1 ori"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     CDS             3332..3991
                     /label="AmpR"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     rep_origin      4089..4771
                     /label="ColE1 origin"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
ORIGIN
        1 gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag
       61 gaagcggccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct
      121 ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt
      181 agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg
      241 gaattgtgag cggataacaa tttcacacag gaaacagcta tgaccatgat tacgccaagc
      301 gcgcaattaa ccctcactaa agggaacaaa agctggagct ccaccgcggt ggcggccgct
      361 ctagaagggc tgcaggaatt cgatattttt gctttcgtcg taaatctaca cacgcgtctc
      421 ttccgtgcga gagtccaagc cagcagccaa attcgttgac tgagtattca acgtttatac
      481 gttgtcggca acgagaaata ggaaaatgca tcgggaaatg ttcttttttc gattttttcc
      541 aaggttttga caaattttac cacgaatttt gctatgtttt caattaaaaa atatgttatt
      601 caactgtttc tatgaggaaa ataaggcttt gcatgtaatt ttcttattca gcataatttt
      661 taattaattt gaattttctg tcctaacgtt tattttgttt tcttggttat gactgatctg
      721 aaattaattt ttgaatttta aggtaatATG AACGGAGCTA TCGGAGGAGA CCTTCTCCTC
      781 AACTTCCCAG ACATGTCCGT CCTCGAGCGT CAACGTGCTC ACCTCAAGTA CCTCAACCCA
      841 ACCTTCGATT CCCCACTCGC CGGATTCTTC GCCGATTCTT CCATGATCAC CGGAGGAGAG
      901 ATGGACTCCT ACCTTTCCAC CGCCGGACTC AATCTTCCAA TGATGTACGG AGAGACCACC
      961 GTCGAGGGAG ATTCCCGTCT CTCCATCTCC CCAGAGACCA CCCTTGGAAC TGGAAACTTC
     1021 AAGGCCGCCA AGTTCGATAC CGAGACTAAG GATTGCAACG AGGCCGCCAA GAAGATGACC
     1081 ATGAACCGTG ATGACCTCGT CGAAGAAGGA GAAGAGGAGA AGTCCAAGAT CACCGAGCAA
     1141 AACAACGGAT CCACCAAGTC CATCAAGAAG ATGAAGCACA AGGCCAAGAA GGAAGAGAAC
     1201 AACTTCTCTA ACGATTCCTC CAAGGTCACC AAGGAGCTCG AGAAGACCGA TTACATCGAT
     1261 TACAAGgtaa gtttaaacat atatatacta actaaccctg attatttaaa ttttcagGAT
     1321 CACGATGGAG ACTACAAGGA CCACGATATC GACTACAAGG ACGATGACGA TAAAGGATCC
     1381 GGAGGtGGAG GtGGcGGATC CGGGACGGGA GGAAAGTCCG GAGGAAACAA GAAGAACGAT
     1441 GGAGTCAAGG AGTCTTCTGA GTCCACCAAC ACCACCATCG AGGACGAGGA CACCAAGGTC
     1501 CGCAAGCAAG AAATCATCAA GGTCACCGAG CAACTCATCG AGGCCATCTC CAACGGAGAC
     1561 TTCGAGTCCT ACACCAAGgt aagtttaaac atatatatac taactaaccc tgattattta
     1621 aattttcagA TGTGCGACCC AGGAATGACC GCCTTCGAGC CAGAGGCCCT CGGAAACCTC
     1681 GTCGAGGGAC TCGACTTCCA CCGTTTCTAC TTCGAAAACC TCTGGTCCCG TAACTCCAAG
     1741 CCAGTCCACA CCACCATCCT CAACCCACAC ATCCACCTCA TGGGAGACGA GTCCGCCTGC
     1801 ATCGCCTACA TCCGCATCAC TCAATACCTC GATGCCGGAG GAATCCCACG CACCGCCCAA
     1861 TCCGAGGAGA CCCGCGTCTG GCACCGCCGT GACGGAAAGT GGCAAATCGT CCACTTCCAC
     1921 CGTTCTGGAG CCCCATCCGT CCTCCCACAC TGCtaatcca attactcttc aacatcccta
     1981 catgctcttt ctccctgtgc tcccaccccc tatttttgtt attatcaaaa aacttctctt
     2041 aatttctttg ttttttagct tcttttaagt cacctctaac aatgaaattg tgtagattca
     2101 aaaatagaat taattcgtaa taaaaagtcg aaaaaaattg tgctccctcc ccccattaat
     2161 aataattcta tcccaaaatc tacacaatgt tctgtgtaca cttcttatgt tttttacttc
     2221 tgataaattt ttttgaaaca tcatagaaaa aaccgcacac aaaatacctt atcatatgtt
     2281 acgtttcagt ttatgaccgc aatttttaat caagcttatc gataccgtcg acctcgaggg
     2341 ggggcccggt acccaattcg ccctatagtg agtcgtatta cgcgcgctca ctggccgtcg
     2401 ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac
     2461 atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac
     2521 agttgcgcag cctgaatggc gaatgggacg cgccctgtag cggcgcatta agcgcggcgg
     2581 gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt
     2641 tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc
     2701 gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg
     2761 attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga
     2821 cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc
     2881 ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa
     2941 aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta acgcttacaa
     3001 tttaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat
     3061 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
     3121 aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc
     3181 attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga
     3241 tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga
     3301 gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg
     3361 cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc
     3421 tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac
     3481 agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact
     3541 tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca
     3601 tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg
     3661 tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact
     3721 acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg
     3781 accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg
     3841 tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat
     3901 cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc
     3961 tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat
     4021 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt
     4081 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
     4141 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
     4201 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
     4261 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt
     4321 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
     4381 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
     4441 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
     4501 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
     4561 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
     4621 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
     4681 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
     4741 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
     4801 ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc
     4861 cttt
//