LOCUS       pENTR_BIP-Myc-Bi        3645 bp    DNA     linear       07-JUL-2021
FEATURES             Location/Qualifiers
     misc_feature    568..668
                     /note="attL1"
     misc_feature    1770..1869
                     /note="attL2"
     misc_feature    537..552
                     /note="M13 F (-20)"
     misc_feature    complement(1910..1926)
                     /note="M13 reverse"
     misc_feature    2039..2848
                     /note="Kanamycin Resistance gene"
     misc_feature    652..1786
                     /note="paste into expression clone"
     CDS             743..772
                     /codon_start=1
                     /label=Myc
                     /note="Myc"
                     /translation="EQKLISEEDL"
     misc_feature    773..778
                     /label=NheI
                     /note="NheI"
     CDS             779..1735
                     /codon_start=1
                     /note="BirA (humanized)"
                     /translation="KDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRD
                     WGVDVFTVPGKGYSLPEPIPLLNAKQILGQLDGGSVAVLPVVDSTNQYLLDRIGELKS
                     GDACIAEYQQAGRGSRGRKWFSPFGANLYLSMFWRLKRGPAAIGLGPVIGIVMAEALR
                     KLGADKVRVKWPNDLYLQDRKLAGILVELAGKTGDAAQIVIGAGINVAMRRVEESVVN
                     QGWITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKL
                     IIGDKEIFGISRGIDKQGALLLEQDGVIKPWMGGEISLRSAEK"
     misc_feature    968..970
                     /label=Q65P
                     /note="Q65P"
     misc_feature    1034..1036
                     /note="I87V"
     misc_feature    1118..1120
                     /label=G115G
                     /note="G115G"
     misc_feature    1127..1129
                     /label=R118S
                     /note="R118S"
     misc_feature    689..742
                     /note="BIP signal sequence"
     misc_feature    685..767
                     /note="BIP_myc_1F"
     misc_feature    1193..1195
                     /note="E141K"
     misc_difference 1196..1198
                     /note="Q142R"
     misc_feature    1220..1222
                     /note="S150G"
     misc_feature    1223..1225
                     /label=L151P
                     /note="L151P"
     misc_feature    1250..1252
                     /note="V160A"
     misc_feature    1346..1348
                     /note="T192A"
     misc_feature    1397..1399
                     /note="M209V"
     misc_structure  1643..1645
                     /note="G291G"
     misc_feature    1685..1687
                     /label=I305V
                     /note="I305V"
     misc_feature    complement(1719..1753)
                     /note="KDEL-BirA*2-1R"
     misc_feature    1736..1747
                     /note="KDEL"
     source          1..3645
                     /dnas_title="pENTR_BIP-Myc-BirA*2-KDEL-stop"
ORIGIN      
        1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA
       61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA
      121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA
      181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC
      241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA
      301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC
      361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA
      421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG
      481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA
      541 AACGACGGCC AGTCTTAAGC TCGGGCCCCA AATAATGATT TTATTTTGAC TGATAGTGAC
      601 CTGTTCGTTG CAACAAATTG ATGAGCAATG CTTTTTTATA ATGCCAACTT TGTACAAAAA
      661 AGCAGGCTCC GCGGCCGCCC CCTTcaccAT GAAGTTATGC ATATTACTGG CCGTCGTGGC
      721 CTTTGTTGGC CTCTCGCTCG GGgaacaaaa gcttatttct gaagaggact tggctagcaa
      781 agacaatact gtgcctctga agctgatcgc tctcctggct aatggcgagt tccatagtgg
      841 cgaacagctg ggagaaaccc tgggcatgtc cagggccgct atcaacaagc acattcagac
      901 tctgcgcgac tggggcgtgg acgtgttcac cgtgcccgga aagggctact ctctgcccga
      961 gcctatcccg ctgctgaacg ctaaacagat tctgggacag ctggacggcg ggagcgtggc
     1021 agtcctgcct gtggtcgact ccaccaatca gtacctgctg gatcgaatcg gcgagctgaa
     1081 gagtggggat gcttgcattg cagaatatca gcaggcaggg agaggaagca gagggaggaa
     1141 atggttctct ccttttggag ctaacctgta cctgagtatg ttttggcgcc tgaagcgggg
     1201 accagcagca atcggcctgg gcccggtcat cggaattgtc atggcagaag cgctgcgaaa
     1261 gctgggagca gacaaggtgc gagtcaaatg gcccaatgac ctgtatctgc aggatagaaa
     1321 gctggcaggc atcctggtgg agctggccgg aaaaacaggc gatgctgcac agatcgtcat
     1381 tggcgccggg attaacgtgg ctatgaggcg cgtggaggaa agcgtggtca atcagggctg
     1441 gatcacactg caggaagcag ggattaacct ggacaggaat actctggccg ctatgctgat
     1501 ccgagagctg cgggcagccc tggaactgtt cgagcaggaa ggcctggctc catatctgtc
     1561 acggtgggag aagctggata acttcatcaa tagacccgtg aagctgatca ttggggacaa
     1621 agagattttc gggattagcc gggggattga taaacaggga gccctgctgc tggaacagga
     1681 cggagttatc aaaccctgga tgggcggaga aatcagtctg cggtctgccg aaaagAAGGA
     1741 CGAGCTGtaa tagAAGGGTG GGCGCGCCGA CCCAGCTTTC TTGTACAAAG TTGGCATTAT
     1801 AAGAAAGCAT TGCTTATCAA TTTGTTGCAA CGAACAGGTC ACTATCAGTC AAAATAAAAT
     1861 CATTATTTGC CATCCAGCTG ATATCCCCTA TAGTGAGTCG TATTACATGG TCATAGCTGT
     1921 TTCCTGGCAG CTCTGGCCCG TGTCTCAAAA TCTCTGATGT TACATTGCAC AAGATAAAAA
     1981 TATATCATCA TGAACAATAA AACTGTCTGC TTACATAAAC AGTAATACAA GGGGTGTTAT
     2041 GAGCCATATT CAACGGGAAA CGTCGAGGCC GCGATTAAAT TCCAACATGG ATGCTGATTT
     2101 ATATGGGTAT AAATGGGCTC GCGATAATGT CGGGCAATCA GGTGCGACAA TCTATCGCTT
     2161 GTATGGGAAG CCCGATGCGC CAGAGTTGTT TCTGAAACAT GGCAAAGGTA GCGTTGCCAA
     2221 TGATGTTACA GATGAGATGG TCAGACTAAA CTGGCTGACG GAATTTATGC CTCTTCCGAC
     2281 CATCAAGCAT TTTATCCGTA CTCCTGATGA TGCATGGTTA CTCACCACTG CGATCCCCGG
     2341 AAAAACAGCA TTCCAGGTAT TAGAAGAATA TCCTGATTCA GGTGAAAATA TTGTTGATGC
     2401 GCTGGCAGTG TTCCTGCGCC GGTTGCATTC GATTCCTGTT TGTAATTGTC CTTTTAACAG
     2461 CGATCGCGTA TTTCGTCTCG CTCAGGCGCA ATCACGAATG AATAACGGTT TGGTTGATGC
     2521 GAGTGATTTT GATGACGAGC GTAATGGCTG GCCTGTTGAA CAAGTCTGGA AAGAAATGCA
     2581 TAAACTTTTG CCATTCTCAC CGGATTCAGT CGTCACTCAT GGTGATTTCT CACTTGATAA
     2641 CCTTATTTTT GACGAGGGGA AATTAATAGG TTGTATTGAT GTTGGACGAG TCGGAATCGC
     2701 AGACCGATAC CAGGATCTTG CCATCCTATG GAACTGCCTC GGTGAGTTTT CTCCTTCATT
     2761 ACAGAAACGG CTTTTTCAAA AATATGGTAT TGATAATCCT GATATGAATA AATTGCAGTT
     2821 TCATTTGATG CTCGATGAGT TTTTCTAATC AGAATTGGTT AATTGGTTGT AACACTGGCA
     2881 GAGCATTACG CTGACTTGAC GGGACGGCGC AAGCTCATGA CCAAAATCCC TTAACGTGAG
     2941 TTACGCGTCG TTCCACTGAG CGTCAGACCC CGTAGAAAAG ATCAAAGGAT CTTCTTGAGA
     3001 TCCTTTTTTT CTGCGCGTAA TCTGCTGCTT GCAAACAAAA AAACCACCGC TACCAGCGGT
     3061 GGTTTGTTTG CCGGATCAAG AGCTACCAAC TCTTTTTCCG AAGGTAACTG GCTTCAGCAG
     3121 AGCGCAGATA CCAAATACTG TCCTTCTAGT GTAGCCGTAG TTAGGCCACC ACTTCAAGAA
     3181 CTCTGTAGCA CCGCCTACAT ACCTCGCTCT GCTAATCCTG TTACCAGTGG CTGCTGCCAG
     3241 TGGCGATAAG TCGTGTCTTA CCGGGTTGGA CTCAAGACGA TAGTTACCGG ATAAGGCGCA
     3301 GCGGTCGGGC TGAACGGGGG GTTCGTGCAC ACAGCCCAGC TTGGAGCGAA CGACCTACAC
     3361 CGAACTGAGA TACCTACAGC GTGAGCATTG AGAAAGCGCC ACGCTTCCCG AAGGGAGAAA
     3421 GGCGGACAGG TATCCGGTAA GCGGCAGGGT CGGAACAGGA GAGCGCACGA GGGAGCTTCC
     3481 AGGGGGAAAC GCCTGGTATC TTTATAGTCC TGTCGGGTTT CGCCACCTCT GACTTGAGCG
     3541 TCGATTTTTG TGATGCTCGT CAGGGGGGCG GAGCCTATGG AAAAACGCCA GCAACGCGGC
     3601 CTTTTTACGG TTCCTGGCCT TTTGCTGGCC TTTTGCTCAC ATGTT
//

