LOCUS       pHI302-mVenus-NE        6349 bp    DNA     circular     08-Jul-2021
SOURCE
  ORGANISM
COMMENT     This file was created by DNADynamo
COMMENT     www.bluetractorsoftware.co.uk
FEATURES             Location/Qualifiers
     CDS             1759..2421
                     /label="4025..4687"
                     /note="LARG-DH"
     CDS             1378..1710
                     /label="2477..2809"
                     /note="sspB"
     CDS             2506..3015
                     /label="4325..4834"
                     /note="iLIDcaax"
     CDS             1330..1359
                     /label="1744..1773"
                     /note="NES"
     CDS             2428..2493
                     /label="P2A"
                     /note="P2A"
     misc_feature    4245..5039
                     /label="CDS neomycin phosphotransferase"
                     /note="neomycin phosphotransferase"
     misc_feature    613..1329
                     /label="CDS mVenus"
                     /note="mVenus"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg 
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac 
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg 
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg 
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt 
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta 
      601 ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg 
      661 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc 
      721 gatgccacct acggcaagct gaccctgaag ttcatcagca ccaccggcaa gctgcccgtg 
      781 ccctggccca ccctcgtgac caccctcggc tacggcctgc agtgcttcgc ccgctacccc 
      841 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag 
      901 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag 
      961 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac 
     1021 atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat caccgccgac 
     1081 aagcagaaga acggcatcaa ggcaaacttc aagatccgcc acaacatcga ggacggcggc 
     1141 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg 
     1201 cccgacaacc actacctgag ctaccagtcc aaactgagca aagaccccaa cgagaagcgc 
     1261 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag 
     1321 ctgtacaagt tcggcattga tctgagcggc ctgaccctgg gtaccagtgc tggaggtagc 
     1381 tccccgaaac gccctaagct gctgcgtgaa tattacgatt ggctggttga taacagcttt 
     1441 accccatatc tggtggtgga tgccacatac ctgggcgtga acgtgcccgt ggagtatgtg 
     1501 aaagacggtc agatcgtgct gaatctgtct gcaagtgcga ccggcaacct gcaactgaca 
     1561 aatgatttta tccagttcaa cgcccgcttt aagggcgtgt ctcgtgaact gtatatcccg 
     1621 atgggtgccg ctctggccat ttacgctcgc gagaacggcg atggtgtgat gttcgaacca 
     1681 gaagaaatct atgacgagct gaatattggt ctcgacagtg ctggaggtag tgctggaggt 
     1741 tcagctggtg gactcgagcc acccaactgg cagcagcttg ttagtcgaga agtgttactg 
     1801 ggactaaaac cttgtgaaat caaaagacag gaagtgatta atgaattgtt ctacactgaa 
     1861 agagctcatg ttcgaacact gaaggttctt gatcaagtgt tctatcagcg agtatccaga 
     1921 gaaggaattc tgtcaccctc agagctacgg aaaatttttt caaacttgga agatattctt 
     1981 caacttcata ttggattgaa tgaacaaatg aaggctgttc gaaagagaaa tgagacctct 
     2041 gttatcgatc agattgggga agatttgctg acatggttca gcggaccagg agaggagaaa 
     2101 ttgaaacatg ctgctgctac cttttgcagt aaccaacctt tcgccctgga aatgatcaaa 
     2161 tctcgtcaga aaaaggattc tcgatttcag acttttgtgc aagatgctga aagtaatcca 
     2221 ctgtgtcgtc gtcttcaact gaaggatatt attcccactc aaatgcaaag gcttactaag 
     2281 tacccacttc tgttggataa tattgccaaa tacacagaat ggccaacaga aagggagaag 
     2341 gtgaagaaag ctgcagatca ctgtcgtcag atcttaaatt atgtaaatca ggctgtcaag 
     2401 gaggcagaaa acaagcagcg cccgcgggga agcggagcta ctaacttcag cctgctgaag 
     2461 caggctggag acgtggagga gaaccctgga cctgcaccgg gatccgggga gtttctggca 
     2521 accacactgg aacggatcga gaaaaatttc gtgattactg atccgagact gcctgacaac 
     2581 ccaatcattt ttgcgagcga ttccttcctg cagctgacag aatattctcg ggaagagatc 
     2641 ctggggcgca attgccgttt tctgcaggga cccgagacag accgtgccac tgttcggaaa 
     2701 atcagagatg ctattgacaa ccagactgaa gtgaccgttc agctgatcaa ttataccaag 
     2761 agcggcaaga agttctggaa cgtgttccac ctgcagccga tgcgcgatta taagggcgac 
     2821 gtccagtact tcattggcgt gcagctggat ggcaccgaac gtcttcatgg cgccgctgag 
     2881 cgtgaggcgg tctgcctgat caaaaagaca gcctttcaga ttgctgaggc agcgaacgac 
     2941 gaaaattact ttggaagcgg gagtgggagc aaaaagaaga aaaagaagtc aaagacaaag 
     3001 tgtgtaatta tgtaggcggc cgcgactcta gatcataatc agccatacca catttgtaga 
     3061 ggttttactt gctttaaaaa acctcccaca cctccccctg aacctgaaac ataaaatgaa 
     3121 tgcaattgtt gttgttaact tgtttattgc agcttataat ggttacaaat aaagcaatag 
     3181 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa 
     3241 actcatcaat gtatcttaag gcgtaaattg taagcgttaa tattttgtta aaattcgcgt 
     3301 taaatttttg ttaaatcagc tcatttttta accaataggc cgaaatcggc aaaatccctt 
     3361 ataaatcaaa agaatagacc gagatagggt tgagtgttgt tccagtttgg aacaagagtc 
     3421 cactattaaa gaacgtggac tccaacgtca aagggcgaaa aaccgtctat cagggcgatg 
     3481 gcccactacg tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac 
     3541 taaatcggaa ccctaaaggg agcccccgat ttagagcttg acggggaaag ccggcgaacg 
     3601 tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag 
     3661 cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt 
     3721 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac 
     3781 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa 
     3841 aaaggaagag tcctgaggcg gaaagaacca gctgtggaat gtgtgtcagt tagggtgtgg 
     3901 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc 
     3961 aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct 
     4021 caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc 
     4081 cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg cagaggccga 
     4141 ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg 
     4201 cttttgcaaa gatcgatcaa gagacaggat gaggatcgtt tcgcatgatt gaacaagatg 
     4261 gattgcacgc aggttctccg gccgcttggg tggagaggct attcggctat gactgggcac 
     4321 aacagacaat cggctgctct gatgccgccg tgttccggct gtcagcgcag gggcgcccgg 
     4381 ttctttttgt caagaccgac ctgtccggtg ccctgaatga actgcaagac gaggcagcgc 
     4441 ggctatcgtg gctggccacg acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg 
     4501 aagcgggaag ggactggctg ctattgggcg aagtgccggg gcaggatctc ctgtcatctc 
     4561 accttgctcc tgccgagaaa gtatccatca tggctgatgc aatgcggcgg ctgcatacgc 
     4621 ttgatccggc tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta 
     4681 ctcggatgga agccggtctt gtcgatcagg atgatctgga cgaagagcat caggggctcg 
     4741 cgccagccga actgttcgcc aggctcaagg cgagcatgcc cgacggcgag gatctcgtcg 
     4801 tgacccatgg cgatgcctgc ttgccgaata tcatggtgga aaatggccgc ttttctggat 
     4861 tcatcgactg tggccggctg ggtgtggcgg accgctatca ggacatagcg ttggctaccc 
     4921 gtgatattgc tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg ctttacggta 
     4981 tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct tcttgacgag ttcttctgag 
     5041 cgggactctg gggttcgaaa tgaccgacca agcgacgccc aacctgccat cacgagattt 
     5101 cgattccacc gccgccttct atgaaaggtt gggcttcgga atcgttttcc gggacgccgg 
     5161 ctggatgatc ctccagcgcg gggatctcat gctggagttc ttcgcccacc ctagggggag 
     5221 gctaactgaa acacggaagg agacaatacc ggaaggaacc cgcgctatga cggcaataaa 
     5281 aagacagaat aaaacgcacg gtgttgggtc gtttgttcat aaacgcgggg ttcggtccca 
     5341 gggctggcac tctgtcgata ccccaccgag accccattgg ggccaatacg cccgcgtttc 
     5401 ttccttttcc ccaccccacc ccccaagttc gggtgaaggc ccagggctcg cagccaacgt 
     5461 cggggcggca ggccctgcca tagcctcagg ttactcatat atactttaga ttgatttaaa 
     5521 acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa 
     5581 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg 
     5641 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc 
     5701 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac 
     5761 tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca 
     5821 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt 
     5881 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc 
     5941 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg 
     6001 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc 
     6061 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac 
     6121 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct 
     6181 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc 
     6241 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt 
     6301 tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gccatgcat
//