LOCUS       pHI447_mVenus-NE        5710 bp    DNA     circular     09-Jul-2021
SOURCE
  ORGANISM
COMMENT     This file was created by DNADynamo
COMMENT     www.bluetractorsoftware.co.uk
FEATURES             Location/Qualifiers
     CDS             1378..1710
                     /label="2477..2809"
                     /note="sspB"
     CDS             1867..2376
                     /label="4325..4834"
                     /note="iLIDcaax"
     CDS             1330..1359
                     /label="1744..1773"
                     /note="NES"
     CDS             1789..1854
                     /label="P2A"
                     /note="P2A"
     misc_feature    3606..4400
                     /label="CDS neomycin phosphotransferase"
                     /note="neomycin phosphotransferase"
     misc_feature    613..1329
                     /label="CDS mVenus"
                     /note="mVenus"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg 
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac 
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg 
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg 
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt 
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta 
      601 ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg 
      661 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc 
      721 gatgccacct acggcaagct gaccctgaag ttgatctgca ccaccggcaa gctgcccgtg 
      781 ccctggccca ccctcgtgac caccctcggc tacggcctgc agtgcttcgc ccgctacccc 
      841 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag 
      901 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgat 
      961 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac 
     1021 atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat caccgccgac 
     1081 aagcagaaga acggcatcaa ggcaaacttc aagatccgcc acaacatcga ggacggcggc 
     1141 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg 
     1201 cccgacaacc actacctgag ctaccagtcc aaactgagca aagaccccaa cgagaagcgc 
     1261 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag 
     1321 ctgtacaagt tcggcattga tctgagcggc ctgaccctgg gtaccagtgc tggaggtagc 
     1381 tccccgaaac gccctaagct gctgcgtgaa tattacgatt ggctggttga taacagcttt 
     1441 accccatatc tggtggtgga tgccacatac ctgggcgtga acgtgcccgt ggagtatgtg 
     1501 aaagacggtc agatcgtgct gaatctgtct gcaagtgcga ccggcaacct gcaactgaca 
     1561 aatgatttta tccagttcaa cgcccgcttt aagggcgtgt ctcgtgaact gtatatcccg 
     1621 atgggtgccg ctctggccat ttacgctcgc gagaacggcg atggtgtgat gttcgaacca 
     1681 gaagaaatct atgacgagct gaatattggt ctcgacagtg ctggaggtag tgctggaggt 
     1741 tcagctggtg gactcgaggt cgacgaattc gagctcgata tcccgcgggg aagcggagct 
     1801 actaacttca gcctgctgaa gcaggctgga gacgtggagg agaaccctgg acctgcaccg 
     1861 ggatccgggg agtttctggc aaccacactg gaacggatcg agaaaaattt cgtgattact 
     1921 gatccgagac tgcctgacaa cccaatcatt tttgcgagcg attccttcct gcagctgaca 
     1981 gaatattctc gggaagagat cctggggcgc aattgccgtt ttctgcaggg acccgagaca 
     2041 gaccgtgcca ctgttcggaa aatcagagat gctattgaca accagactga agtgaccgtt 
     2101 cagctgatca attataccaa gagcggcaag aagttctgga acgtgttcca cctgcagccg 
     2161 atgcgcgatt ataagggcga cgtccagtac ttcattggcg tgcagctgga tggcaccgaa 
     2221 cgtcttcatg gcgccgctga gcgtgaggcg gtctgcctga tcaaaaagac agcctttcag 
     2281 attgctgagg cagcgaacga cgaaaattac tttggaagcg ggagtgggag caaaaagaag 
     2341 aaaaagaagt caaagacaaa gtgtgtaatt atgtaggcgg ccgcgactct agatcataat 
     2401 cagccatacc acatttgtag aggttttact tgctttaaaa aacctcccac acctccccct 
     2461 gaacctgaaa cataaaatga atgcaattgt tgttgttaac ttgtttattg cagcttataa 
     2521 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca 
     2581 ttctagttgt ggtttgtcca aactcatcaa tgtatcttaa ggcgtaaatt gtaagcgtta 
     2641 atattttgtt aaaattcgcg ttaaattttt gttaaatcag ctcatttttt aaccaatagg 
     2701 ccgaaatcgg caaaatccct tataaatcaa aagaatagac cgagataggg ttgagtgttg 
     2761 ttccagtttg gaacaagagt ccactattaa agaacgtgga ctccaacgtc aaagggcgaa 
     2821 aaaccgtcta tcagggcgat ggcccactac gtgaaccatc accctaatca agttttttgg 
     2881 ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg gagcccccga tttagagctt 
     2941 gacggggaaa gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg 
     3001 ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta 
     3061 atgcgccgct acagggcgcg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta 
     3121 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat 
     3181 aaatgcttca ataatattga aaaaggaaga gtcctgaggc ggaaagaacc agctgtggaa 
     3241 tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag 
     3301 catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag 
     3361 aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc 
     3421 catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt 
     3481 ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg 
     3541 aggctttttt ggaggcctag gcttttgcaa agatcgatca agagacagga tgaggatcgt 
     3601 ttcgcatgat tgaacaagat ggattgcacg caggttctcc ggccgcttgg gtggagaggc 
     3661 tattcggcta tgactgggca caacagacaa tcggctgctc tgatgccgcc gtgttccggc 
     3721 tgtcagcgca ggggcgcccg gttctttttg tcaagaccga cctgtccggt gccctgaatg 
     3781 aactgcaaga cgaggcagcg cggctatcgt ggctggccac gacgggcgtt ccttgcgcag 
     3841 ctgtgctcga cgttgtcact gaagcgggaa gggactggct gctattgggc gaagtgccgg 
     3901 ggcaggatct cctgtcatct caccttgctc ctgccgagaa agtatccatc atggctgatg 
     3961 caatgcggcg gctgcatacg cttgatccgg ctacctgccc attcgaccac caagcgaaac 
     4021 atcgcatcga gcgagcacgt actcggatgg aagccggtct tgtcgatcag gatgatctgg 
     4081 acgaagagca tcaggggctc gcgccagccg aactgttcgc caggctcaag gcgagcatgc 
     4141 ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat atcatggtgg 
     4201 aaaatggccg cttttctgga ttcatcgact gtggccggct gggtgtggcg gaccgctatc 
     4261 aggacatagc gttggctacc cgtgatattg ctgaagagct tggcggcgaa tgggctgacc 
     4321 gcttcctcgt gctttacggt atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc 
     4381 ttcttgacga gttcttctga gcgggactct ggggttcgaa atgaccgacc aagcgacgcc 
     4441 caacctgcca tcacgagatt tcgattccac cgccgccttc tatgaaaggt tgggcttcgg 
     4501 aatcgttttc cgggacgccg gctggatgat cctccagcgc ggggatctca tgctggagtt 
     4561 cttcgcccac cctaggggga ggctaactga aacacggaag gagacaatac cggaaggaac 
     4621 ccgcgctatg acggcaataa aaagacagaa taaaacgcac ggtgttgggt cgtttgttca 
     4681 taaacgcggg gttcggtccc agggctggca ctctgtcgat accccaccga gaccccattg 
     4741 gggccaatac gcccgcgttt cttccttttc cccaccccac cccccaagtt cgggtgaagg 
     4801 cccagggctc gcagccaacg tcggggcggc aggccctgcc atagcctcag gttactcata 
     4861 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct 
     4921 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga 
     4981 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg 
     5041 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc 
     5101 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct 
     5161 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc 
     5221 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt 
     5281 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg 
     5341 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct 
     5401 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag 
     5461 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag 
     5521 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg 
     5581 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg 
     5641 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac 
     5701 cgccatgcat 
//