LOCUS       mVenus-NES-SspB-        7426 bp    DNA     circular     09-Jul-2021
SOURCE
  ORGANISM
COMMENT     This file was created by DNADynamo
COMMENT     www.bluetractorsoftware.co.uk
FEATURES             Location/Qualifiers
     CDS             1378..1710
                     /label="2477..2809"
                     /note="sspB"
     CDS             3583..4092
                     /label="4325..4834"
                     /note="iLIDcaax"
     CDS             1759..3498
                     /label="Tiam-DH-PH"
                     /note="Tiam-DH-PH"
     CDS             1330..1359
                     /label="1744..1773"
                     /note="NES"
     CDS             3505..3570
                     /label="P2A"
                     /note="P2A"
     misc_feature    5322..6116
                     /label="CDS neomycin phosphotransferase"
                     /note="neomycin phosphotransferase"
     misc_feature    613..1329
                     /label="CDS mVenus"
                     /note="mVenus"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg 
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac 
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg 
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg 
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt 
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta 
      601 ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg 
      661 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc 
      721 gatgccacct acggcaagct gaccctgaag ttgatctgca ccaccggcaa gctgcccgtg 
      781 ccctggccca ccctcgtgac caccctcggc tacggcctgc agtgcttcgc ccgctacccc 
      841 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag 
      901 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgat 
      961 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac 
     1021 atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat caccgccgac 
     1081 aagcagaaga acggcatcaa ggcaaacttc aagatccgcc acaacatcga ggacggcggc 
     1141 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg 
     1201 cccgacaacc actacctgag ctaccagtcc aaactgagca aagaccccaa cgagaagcgc 
     1261 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag 
     1321 ctgtacaagt tcggcattga tctgagcggc ctgaccctgg gtaccagtgc tggaggtagc 
     1381 tccccgaaac gccctaagct gctgcgtgaa tattacgatt ggctggttga taacagcttt 
     1441 accccatatc tggtggtgga tgccacatac ctgggcgtga acgtgcccgt ggagtatgtg 
     1501 aaagacggtc agatcgtgct gaatctgtct gcaagtgcga ccggcaacct gcaactgaca 
     1561 aatgatttta tccagttcaa cgcccgcttt aagggcgtgt ctcgtgaact gtatatcccg 
     1621 atgggtgccg ctctggccat ttacgctcgc gagaacggcg atggtgtgat gttcgaacca 
     1681 gaagaaatct atgacgagct gaatattggt ctcgacagtg ctggaggtag tgctggaggt 
     1741 tcagctggtg gactcgagat gaacccctct gaccagagcc catctcctca ggactccacg 
     1801 gggcctcagc tggcgaccat gagacaactc tcggatgcag ataagctgcg caaggtgatc 
     1861 tgcgagctcc tggagacgga gcgcacctac gtgaaggatt taaactgtct tatggagaga 
     1921 tacctaaagc ctcttcaaaa agaaactttt ctcacccagg atgagcttga cgtgcttttt 
     1981 ggaaatttaa cggaaatggt agagtttcaa gtagaattcc ttaaaactct agaagatgga 
     2041 gtgagactgg tacctgattt ggaaaagctt gagaaggttg atcaatttaa gaaagtgctg 
     2101 ttctctctgg ggggatcatt cctgtattat gctgaccgct tcaagctcta cagtgccttc 
     2161 tgcgccagcc acacaaaagt tcccaaggtc ctggtgaaag ccaagacaga cacggctttc 
     2221 aaggcattct tggatgccca gaacccgaag cagcagcact catccacgct ggagtcgtac 
     2281 ctcatcaagc ccatccagag gatcctcaag tacccacttc tgctcaggga gctgttcgcc 
     2341 ctgaccgatg cggagagcga ggagcactac cacctggacg tggccatcaa gaccatgaac 
     2401 aaggttgcca gtcacatcaa tgagatgcag aaaatccatg aagagtttgg ggctgtgttt 
     2461 gaccagctga ttgctgaaca gactggtggg aaaaaagagg ttgcagatct gagcatggga 
     2521 gacctgcttt tgcacactac cgtgatctgg ctgaacccgc cggcctcgct gggcaagtgg 
     2581 aaaaaggaac cagagttggc agcattcgtc ttcaaaactg ctgtggtcct tgtgtataaa 
     2641 gatggttcca aacagaagaa gaaacttgta ggatctcaca ggctttccat ttatgaggac 
     2701 tgggacccct tcagatttcg acacatgatc cccacggaag cgctgcaggt tcgagctttg 
     2761 gcgagtgcag atgcagaggc aaatgccgtg tgtgaaattg tccatgtaaa atccgagtct 
     2821 gaagggaggc cggagagggt ctttcacttg tgctgcagct ccccagagag ccgaaaggat 
     2881 ttcctaaagg ctgtgcattc aatcctgcgt gataagcaca gaagacagct cctcaaaacc 
     2941 gagagccttc cctcatccca gcaatatgtc ccttttggag gcaaaagatt gtgtgcactg 
     3001 aagggggcca ggccggccat gagcagggca gtgtctgccc caagcaagtc tcttgggagg 
     3061 aggaggcggc ggctggctcg aaacaggttt accattgatt ctgatgccgt ctccgcaagc 
     3121 agcccggaga aagagtccca gcagcccccc ggtggcgggg acactgaccg atgggtagag 
     3181 gagcagtttg atcttgctca gtatgaggag caagatgaca tcaaggagac agacatcctc 
     3241 agtgacgatg atgagttctg tgagtccgtg aagggtgcct cagtggacag agacctgcag 
     3301 gagcggcttc aggccacctc catcagtcag cgggaaagag gccggaaaac cctggatagt 
     3361 cacgcgtccc gcatggcaca gctcaagaag caagctgccc tgtcggggat caatggaggc 
     3421 ctggagagcg caagcgagga agtcatttgg gttaggcgtg aagactttgc cccctccagg 
     3481 aaactgaaca ctgagatccc gcggggaagc ggagctacta acttcagcct gctgaagcag 
     3541 gctggagacg tggaggagaa ccctggacct gcaccgggat ccggggagtt tctggcaacc 
     3601 acactggaac ggatcgagaa aaatttcgtg attactgatc cgagactgcc tgacaaccca 
     3661 atcatttttg cgagcgattc cttcctgcag ctgacagaat attctcggga agagatcctg 
     3721 gggcgcaatt gccgttttct gcagggaccc gagacagacc gtgccactgt tcggaaaatc 
     3781 agagatgcta ttgacaacca gactgaagtg accgttcagc tgatcaatta taccaagagc 
     3841 ggcaagaagt tctggaacgt gttccacctg cagccgatgc gcgattataa gggcgacgtc 
     3901 cagtacttca ttggcgtgca gctggatggc accgaacgtc ttcatggcgc cgctgagcgt 
     3961 gaggcggtct gcctgatcaa aaagacagcc tttcagattg ctgaggcagc gaacgacgaa 
     4021 aattactttg gaagcgggag tgggagcaaa aagaagaaaa agaagtcaaa gacaaagtgt 
     4081 gtaattatgt aggcggccgc gactctagat cataatcagc cataccacat ttgtagaggt 
     4141 tttacttgct ttaaaaaacc tcccacacct ccccctgaac ctgaaacata aaatgaatgc 
     4201 aattgttgtt gttaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat 
     4261 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact 
     4321 catcaatgta tcttaaggcg taaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa 
     4381 atttttgtta aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata 
     4441 aatcaaaaga atagaccgag atagggttga gtgttgttcc agtttggaac aagagtccac 
     4501 tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc 
     4561 cactacgtga accatcaccc taatcaagtt ttttggggtc gaggtgccgt aaagcactaa 
     4621 atcggaaccc taaagggagc ccccgattta gagcttgacg gggaaagccg gcgaacgtgg 
     4681 cgagaaagga agggaagaaa gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg 
     4741 tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc gccgctacag ggcgcgtcag 
     4801 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt 
     4861 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa 
     4921 ggaagagtcc tgaggcggaa agaaccagct gtggaatgtg tgtcagttag ggtgtggaaa 
     4981 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac 
     5041 caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa 
     5101 ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag 
     5161 ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc 
     5221 cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt 
     5281 ttgcaaagat cgatcaagag acaggatgag gatcgtttcg catgattgaa caagatggat 
     5341 tgcacgcagg ttctccggcc gcttgggtgg agaggctatt cggctatgac tgggcacaac 
     5401 agacaatcgg ctgctctgat gccgccgtgt tccggctgtc agcgcagggg cgcccggttc 
     5461 tttttgtcaa gaccgacctg tccggtgccc tgaatgaact gcaagacgag gcagcgcggc 
     5521 tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag 
     5581 cgggaaggga ctggctgcta ttgggcgaag tgccggggca ggatctcctg tcatctcacc 
     5641 ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat gcggcggctg catacgcttg 
     5701 atccggctac ctgcccattc gaccaccaag cgaaacatcg catcgagcga gcacgtactc 
     5761 ggatggaagc cggtcttgtc gatcaggatg atctggacga agagcatcag gggctcgcgc 
     5821 cagccgaact gttcgccagg ctcaaggcga gcatgcccga cggcgaggat ctcgtcgtga 
     5881 cccatggcga tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt tctggattca 
     5941 tcgactgtgg ccggctgggt gtggcggacc gctatcagga catagcgttg gctacccgtg 
     6001 atattgctga agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg 
     6061 ccgctcccga ttcgcagcgc atcgccttct atcgccttct tgacgagttc ttctgagcgg 
     6121 gactctgggg ttcgaaatga ccgaccaagc gacgcccaac ctgccatcac gagatttcga 
     6181 ttccaccgcc gccttctatg aaaggttggg cttcggaatc gttttccggg acgccggctg 
     6241 gatgatcctc cagcgcgggg atctcatgct ggagttcttc gcccacccta gggggaggct 
     6301 aactgaaaca cggaaggaga caataccgga aggaacccgc gctatgacgg caataaaaag 
     6361 acagaataaa acgcacggtg ttgggtcgtt tgttcataaa cgcggggttc ggtcccaggg 
     6421 ctggcactct gtcgataccc caccgagacc ccattggggc caatacgccc gcgtttcttc 
     6481 cttttcccca ccccaccccc caagttcggg tgaaggccca gggctcgcag ccaacgtcgg 
     6541 ggcggcaggc cctgccatag cctcaggtta ctcatatata ctttagattg atttaaaact 
     6601 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat 
     6661 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc 
     6721 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct 
     6781 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg 
     6841 cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca 
     6901 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc 
     6961 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga 
     7021 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac 
     7081 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga 
     7141 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag 
     7201 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg 
     7261 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag 
     7321 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc 
     7381 tgcgttatcc cctgattctg tggataaccg tattaccgcc atgcat
//