LOCUS       mVenus-NES-SspB-        6739 bp    DNA     circular     09-Jul-2021
SOURCE
  ORGANISM
COMMENT     This file was created by DNADynamo
COMMENT     www.bluetractorsoftware.co.uk
FEATURES             Location/Qualifiers
     CDS             1378..1710
                     /label="2477..2809"
                     /note="sspB"
     CDS             2896..3405
                     /label="4325..4834"
                     /note="iLIDcaax"
     CDS             1330..1359
                     /label="1744..1773"
                     /note="NES"
     CDS             1759..2811
                     /label="ITSN1-DH-PH"
                     /note="ITSN1-DH-PH"
     CDS             2818..2883
                     /label="P2A"
                     /note="P2A"
     misc_feature    4635..5429
                     /label="CDS neomycin phosphotransferase"
                     /note="neomycin phosphotransferase"
     misc_feature    613..1329
                     /label="CDS mVenus"
                     /note="mVenus"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg 
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac 
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg 
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg 
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt 
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta 
      601 ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg 
      661 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc 
      721 gatgccacct acggcaagct gaccctgaag ttcatcagca ccaccggcaa gctgcccgtg 
      781 ccctggccca ccctcgtgac caccctcggc tacggcctgc agtgcttcgc ccgctacccc 
      841 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag 
      901 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag 
      961 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac 
     1021 atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat caccgccgac 
     1081 aagcagaaga acggcatcaa ggcaaacttc aagatccgcc acaacatcga ggacggcggc 
     1141 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg 
     1201 cccgacaacc actacctgag ctaccagtcc aaactgagca aagaccccaa cgagaagcgc 
     1261 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag 
     1321 ctgtacaagt tcggcattga tctgagcggc ctgaccctgg gtaccagtgc tggaggtagc 
     1381 tccccgaaac gccctaagct gctgcgtgaa tattacgatt ggctggttga taacagcttt 
     1441 accccatatc tggtggtgga tgccacatac ctgggcgtga acgtgcccgt ggagtatgtg 
     1501 aaagacggtc agatcgtgct gaatctgtct gcaagtgcga ccggcaacct gcaactgaca 
     1561 aatgatttta tccagttcaa cgcccgcttt aagggcgtgt ctcgtgaact gtatatcccg 
     1621 atgggtgccg ctctggccat ttacgctcgc gagaacggcg atggtgtgat gttcgaacca 
     1681 gaagaaatct atgacgagct gaatattggt ctcgacagtg ctggaggtag tgctggaggt 
     1741 tcagctggtg gactcgagat gttgacccca actgaaagaa agcgacaagg atacatccac 
     1801 gagctcattg tcaccgagga gaactatgtg aatgacctgc agctggtcac agagattttt 
     1861 caaaaacccc tgatggagtc tgagctgctg acagaaaaag aggttgctat gatttttgtg 
     1921 aactggaagg agctgattat gtgtaatatc aaactactaa aagcgctgag agtccgcaag 
     1981 aagatgtccg gggagaagat gcctgtgaag atgattggag acatcctgag cgcacagctg 
     2041 ccgcacatgc agccctacat ccgcttctgc agccgccagc tcaacggggc tgccctgatc 
     2101 cagcagaaga cggatgaggc cccagacttc aaggagttcg tcaaaagatt ggcaatggat 
     2161 cctcggtgta aagggatgcc actctctagt tttatactga agcctatgca acgggtaaca 
     2221 agatacccac tgatcattaa aaatatcctg gaaaacaccc ctgaaaacca cccggaccac 
     2281 agccacttga agcacgccct ggagaaggcg gaagagctct gttcccaggt gaacgaaggg 
     2341 gtgcgggaga aggagaactc tgaccggctg gagtggatcc aggcccacgt gcagtgtgaa 
     2401 ggcctgtctg agcaacttgt gttcaattca gtgaccaatt gcttggggcc gcgcaaattt 
     2461 ctgcacagtg ggaagctcta caaggccaag agcaacaagg agctgtatgg cttccttttc 
     2521 aacgacttcc tcctgctgac tcagatcacg aagcctttgg ggtcttctgg caccgacaaa 
     2581 gtcttcagcc ccaaatcaaa cctgcagtat aaaatgtata aaacacctat tttcctaaat 
     2641 gaggttctag taaaattacc caccgaccct tctggagacg agcccatctt ccacatctcc 
     2701 cacattgacc gcgtctatac tctccgagca gaaagcataa atgaaaggac tgcctgggtg 
     2761 cagaaaatca aagctgcttc tgaactctac atagagactg agaaaaagaa gccgcgggga 
     2821 agcggagcta ctaacttcag cctgctgaag caggctggag acgtggagga gaaccctgga 
     2881 cctgcaccgg gatccgggga gtttctggca accacactgg aacggatcga gaaaaatttc 
     2941 gtgattactg atccgagact gcctgacaac ccaatcattt ttgcgagcga ttccttcctg 
     3001 cagctgacag aatattctcg ggaagagatc ctggggcgca attgccgttt tctgcaggga 
     3061 cccgagacag accgtgccac tgttcggaaa atcagagatg ctattgacaa ccagactgaa 
     3121 gtgaccgttc agctgatcaa ttataccaag agcggcaaga agttctggaa cgtgttccac 
     3181 ctgcagccga tgcgcgatta taagggcgac gtccagtact tcattggcgt gcagctggat 
     3241 ggcaccgaac gtcttcatgg cgccgctgag cgtgaggcgg tctgcctgat caaaaagaca 
     3301 gcctttcaga ttgctgaggc agcgaacgac gaaaattact ttggaagcgg gagtgggagc 
     3361 aaaaagaaga aaaagaagtc aaagacaaag tgtgtaatta tgtaggcggc cgcgactcta 
     3421 gatcataatc agccatacca catttgtaga ggttttactt gctttaaaaa acctcccaca 
     3481 cctccccctg aacctgaaac ataaaatgaa tgcaattgtt gttgttaact tgtttattgc 
     3541 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt 
     3601 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttaag gcgtaaattg 
     3661 taagcgttaa tattttgtta aaattcgcgt taaatttttg ttaaatcagc tcatttttta 
     3721 accaataggc cgaaatcggc aaaatccctt ataaatcaaa agaatagacc gagatagggt 
     3781 tgagtgttgt tccagtttgg aacaagagtc cactattaaa gaacgtggac tccaacgtca 
     3841 aagggcgaaa aaccgtctat cagggcgatg gcccactacg tgaaccatca ccctaatcaa 
     3901 gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg agcccccgat 
     3961 ttagagcttg acggggaaag ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag 
     4021 gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc accacacccg 
     4081 ccgcgcttaa tgcgccgcta cagggcgcgt caggtggcac ttttcgggga aatgtgcgcg 
     4141 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat 
     4201 aaccctgata aatgcttcaa taatattgaa aaaggaagag tcctgaggcg gaaagaacca 
     4261 gctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag 
     4321 tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc 
     4381 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct 
     4441 aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg 
     4501 actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa 
     4561 gtagtgagga ggcttttttg gaggcctagg cttttgcaaa gatcgatcaa gagacaggat 
     4621 gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc aggttctccg gccgcttggg 
     4681 tggagaggct attcggctat gactgggcac aacagacaat cggctgctct gatgccgccg 
     4741 tgttccggct gtcagcgcag gggcgcccgg ttctttttgt caagaccgac ctgtccggtg 
     4801 ccctgaatga actgcaagac gaggcagcgc ggctatcgtg gctggccacg acgggcgttc 
     4861 cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag ggactggctg ctattgggcg 
     4921 aagtgccggg gcaggatctc ctgtcatctc accttgctcc tgccgagaaa gtatccatca 
     4981 tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc tacctgccca ttcgaccacc 
     5041 aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga agccggtctt gtcgatcagg 
     5101 atgatctgga cgaagagcat caggggctcg cgccagccga actgttcgcc aggctcaagg 
     5161 cgagcatgcc cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata 
     5221 tcatggtgga aaatggccgc ttttctggat tcatcgactg tggccggctg ggtgtggcgg 
     5281 accgctatca ggacatagcg ttggctaccc gtgatattgc tgaagagctt ggcggcgaat 
     5341 gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag cgcatcgcct 
     5401 tctatcgcct tcttgacgag ttcttctgag cgggactctg gggttcgaaa tgaccgacca 
     5461 agcgacgccc aacctgccat cacgagattt cgattccacc gccgccttct atgaaaggtt 
     5521 gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg gggatctcat 
     5581 gctggagttc ttcgcccacc ctagggggag gctaactgaa acacggaagg agacaatacc 
     5641 ggaaggaacc cgcgctatga cggcaataaa aagacagaat aaaacgcacg gtgttgggtc 
     5701 gtttgttcat aaacgcgggg ttcggtccca gggctggcac tctgtcgata ccccaccgag 
     5761 accccattgg ggccaatacg cccgcgtttc ttccttttcc ccaccccacc ccccaagttc 
     5821 gggtgaaggc ccagggctcg cagccaacgt cggggcggca ggccctgcca tagcctcagg 
     5881 ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt 
     5941 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg 
     6001 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt 
     6061 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca 
     6121 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac 
     6181 tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac 
     6241 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct 
     6301 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg 
     6361 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca 
     6421 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt 
     6481 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta 
     6541 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc 
     6601 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc 
     6661 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa 
     6721 ccgtattacc gccatgcat
//