LOCUS       pHI303-mVenus-NE        7144 bp    DNA     circular     08-Jul-2021
SOURCE
  ORGANISM
COMMENT     This file was created by DNADynamo
COMMENT     www.bluetractorsoftware.co.uk
FEATURES             Location/Qualifiers
     CDS             1378..1710
                     /label="2477..2809"
                     /note="sspB"
     CDS             3301..3810
                     /label="4325..4834"
                     /note="iLIDcaax"
     CDS             1330..1359
                     /label="1744..1773"
                     /note="NES"
     CDS             1759..3216
                     /label="4025..4687*1"
                     /note="SOS2cat"
     CDS             3223..3288
                     /label="P2A"
                     /note="P2A"
     misc_feature    5040..5834
                     /label="CDS neomycin phosphotransferase"
                     /note="neomycin phosphotransferase"
     misc_feature    613..1329
                     /label="CDS mVenus"
                     /note="mVenus"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg 
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac 
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg 
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg 
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt 
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta 
      601 ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg 
      661 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc 
      721 gatgccacct acggcaagct gaccctgaag ttcatcagca ccaccggcaa gctgcccgtg 
      781 ccctggccca ccctcgtgac caccctcggc tacggcctgc agtgcttcgc ccgctacccc 
      841 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag 
      901 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag 
      961 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac 
     1021 atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat caccgccgac 
     1081 aagcagaaga acggcatcaa ggcaaacttc aagatccgcc acaacatcga ggacggcggc 
     1141 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg 
     1201 cccgacaacc actacctgag ctaccagtcc aaactgagca aagaccccaa cgagaagcgc 
     1261 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag 
     1321 ctgtacaagt tcggcattga tctgagcggc ctgaccctgg gtaccagtgc tggaggtagc 
     1381 tccccgaaac gccctaagct gctgcgtgaa tattacgatt ggctggttga taacagcttt 
     1441 accccatatc tggtggtgga tgccacatac ctgggcgtga acgtgcccgt ggagtatgtg 
     1501 aaagacggtc agatcgtgct gaatctgtct gcaagtgcga ccggcaacct gcaactgaca 
     1561 aatgatttta tccagttcaa cgcccgcttt aagggcgtgt ctcgtgaact gtatatcccg 
     1621 atgggtgccg ctctggccat ttacgctcgc gagaacggcg atggtgtgat gttcgaacca 
     1681 gaagaaatct atgacgagct gaatattggt ctcgacagtg ctggaggtag tgctggaggt 
     1741 tcagctggtg gactcgagca accactgaga ttaccaagtc ctgaagtata tcgttttgta 
     1801 gtaaaagact ctgaggaaaa cattgttttt gaagacaact tgcaaagtag aagtggcatc 
     1861 cccattatta aaggaggaac tgtagtgaaa ttaattgaaa ggttaacata tcatatgtat 
     1921 gcagatccca attttgttcg tacttttctt accacatatc gttcattttg taaaccacag 
     1981 gaattgctga gcttactgat tgaacggttt gaaattccag agccagaacc tactgacgca 
     2041 gacaaattgg caatagagaa aggcgagcag ccaatcagtg cagaccttaa aagatttcgc 
     2101 aaggaatatg tccaaccagt acaacttagg atcttaaatg tatttcggca ttgggttgaa 
     2161 catcattttt atgactttga aagagacttg gaattgcttg aaagactaga atccttcatt 
     2221 tcaagtgtaa gagggaaagc tatgaaaaaa tgggtagagt caattgctaa gatcatcagg 
     2281 aggaagaagc aagctcaggc aaacggagta agccataata ttacctttga aagtccacct 
     2341 ccaccaattg aatggcatat cagcaaacca ggacagtttg aaacatttga tctcatgaca 
     2401 cttcatccaa tagaaattgc acgtcagctg acacttttgg agtctgatct ttacaggaaa 
     2461 gttcaaccgt ctgaacttgt agggagtgtg tggaccaaag aagataaaga aataaattct 
     2521 ccaaatttat taaaaatgat tcgccatacc acaaatctca ccctctggtt tgaaaaatgc 
     2581 attgtggaag cagaaaattt tgaagaacgg gtggcagtac taagtagaat tatagaaatt 
     2641 ctgcaagttt ttcaagattt gaataatttc aatggcgtat tggagatagt cagtgcagta 
     2701 aattcagtgt cagtatacag actagaccat acctttgagg cactgcagga aaggaaaagg 
     2761 aaaattttgg acgaagctgt ggaattaagt caagatcact ttaaaaaata cctagtaaaa 
     2821 cttaagtcaa tcaatccacc ttgtgtgcct ttttttggaa tatatttaac aaatattctg 
     2881 aagaccgaag aagggaataa tgatttttta aaaaagaaag ggaaagattt aatcaatttc 
     2941 agtaagagga ggaaagtagc tgaaattact ggagaaattc agcagtatca gaatcagcct 
     3001 tactgtttac ggatagaacc agatatgagg agattctttg aaaaccttaa ccccatggga 
     3061 agtgcatctg aaaaagagtt tacagattat ttgttcaaca agtcactaga aattgaacct 
     3121 cgaaactgca aacagccacc tcgatttcct aggaaatcaa ctttttcctt aaaatctcct 
     3181 ggaataaggc ctaacacagg ccgacatggc tctaccccgc ggggaagcgg agctactaac 
     3241 ttcagcctgc tgaagcaggc tggagacgtg gaggagaacc ctggacctgc accgggatcc 
     3301 ggggagtttc tggcaaccac actggaacgg atcgagaaaa atttcgtgat tactgatccg 
     3361 agactgcctg acaacccaat catttttgcg agcgattcct tcctgcagct gacagaatat 
     3421 tctcgggaag agatcctggg gcgcaattgc cgttttctgc agggacccga gacagaccgt 
     3481 gccactgttc ggaaaatcag agatgctatt gacaaccaga ctgaagtgac cgttcagctg 
     3541 atcaattata ccaagagcgg caagaagttc tggaacgtgt tccacctgca gccgatgcgc 
     3601 gattataagg gcgacgtcca gtacttcatt ggcgtgcagc tggatggcac cgaacgtctt 
     3661 catggcgccg ctgagcgtga ggcggtctgc ctgatcaaaa agacagcctt tcagattgct 
     3721 gaggcagcga acgacgaaaa ttactttgga agcgggagtg ggagcaaaaa gaagaaaaag 
     3781 aagtcaaaga caaagtgtgt aattatgtag gcggccgcga ctctagatca taatcagcca 
     3841 taccacattt gtagaggttt tacttgcttt aaaaaacctc ccacacctcc ccctgaacct 
     3901 gaaacataaa atgaatgcaa ttgttgttgt taacttgttt attgcagctt ataatggtta 
     3961 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag 
     4021 ttgtggtttg tccaaactca tcaatgtatc ttaaggcgta aattgtaagc gttaatattt 
     4081 tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa 
     4141 tcggcaaaat cccttataaa tcaaaagaat agaccgagat agggttgagt gttgttccag 
     4201 tttggaacaa gagtccacta ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg 
     4261 tctatcaggg cgatggccca ctacgtgaac catcacccta atcaagtttt ttggggtcga 
     4321 ggtgccgtaa agcactaaat cggaacccta aagggagccc ccgatttaga gcttgacggg 
     4381 gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg 
     4441 cgctggcaag tgtagcggtc acgctgcgcg taaccaccac acccgccgcg cttaatgcgc 
     4501 cgctacaggg cgcgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt 
     4561 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc 
     4621 ttcaataata ttgaaaaagg aagagtcctg aggcggaaag aaccagctgt ggaatgtgtg 
     4681 tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca 
     4741 tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat 
     4801 gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc 
     4861 gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat 
     4921 ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt 
     4981 ttttggaggc ctaggctttt gcaaagatcg atcaagagac aggatgagga tcgtttcgca 
     5041 tgattgaaca agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg 
     5101 gctatgactg ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag 
     5161 cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc 
     5221 aagacgaggc agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc 
     5281 tcgacgttgt cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg 
     5341 atctcctgtc atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc 
     5401 ggcggctgca tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca 
     5461 tcgagcgagc acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag 
     5521 agcatcaggg gctcgcgcca gccgaactgt tcgccaggct caaggcgagc atgcccgacg 
     5581 gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg gtggaaaatg 
     5641 gccgcttttc tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca 
     5701 tagcgttggc tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc 
     5761 tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg 
     5821 acgagttctt ctgagcggga ctctggggtt cgaaatgacc gaccaagcga cgcccaacct 
     5881 gccatcacga gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt 
     5941 tttccgggac gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc 
     6001 ccaccctagg gggaggctaa ctgaaacacg gaaggagaca ataccggaag gaacccgcgc 
     6061 tatgacggca ataaaaagac agaataaaac gcacggtgtt gggtcgtttg ttcataaacg 
     6121 cggggttcgg tcccagggct ggcactctgt cgatacccca ccgagacccc attggggcca 
     6181 atacgcccgc gtttcttcct tttccccacc ccacccccca agttcgggtg aaggcccagg 
     6241 gctcgcagcc aacgtcgggg cggcaggccc tgccatagcc tcaggttact catatatact 
     6301 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga 
     6361 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt 
     6421 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca 
     6481 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct 
     6541 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta 
     6601 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct 
     6661 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc 
     6721 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca 
     6781 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga 
     6841 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg 
     6901 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt 
     6961 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag 
     7021 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt 
     7081 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgccat 
     7141 gcat
//