LOCUS       pHI323_mVenus-NE        6859 bp    DNA     circular     09-Jul-2021
SOURCE
  ORGANISM
COMMENT     This file was created by DNADynamo
COMMENT     www.bluetractorsoftware.co.uk
FEATURES             Location/Qualifiers
     CDS             1378..1710
                     /label="2477..2809"
                     /note="sspB"
     CDS             3016..3525
                     /label="4325..4834"
                     /note="iLIDcaax"
     CDS             1330..1359
                     /label="1744..1773"
                     /note="NES"
     CDS             1759..2931
                     /label="4025..4687*1"
                     /note="C3Gcat"
     CDS             2938..3003
                     /label="P2A"
                     /note="P2A"
     misc_feature    4755..5549
                     /label="CDS neomycin phosphotransferase"
                     /note="neomycin phosphotransferase"
     misc_feature    613..1329
                     /label="CDS mVenus"
                     /note="mVenus"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg 
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac 
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg 
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg 
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt 
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta 
      601 ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg 
      661 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc 
      721 gatgccacct acggcaagct gaccctgaag ttcatcagca ccaccggcaa gctgcccgtg 
      781 ccctggccca ccctcgtgac caccctcggc tacggcctgc agtgcttcgc ccgctacccc 
      841 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag 
      901 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag 
      961 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac 
     1021 atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat caccgccgac 
     1081 aagcagaaga acggcatcaa ggcaaacttc aagatccgcc acaacatcga ggacggcggc 
     1141 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg 
     1201 cccgacaacc actacctgag ctaccagtcc aaactgagca aagaccccaa cgagaagcgc 
     1261 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag 
     1321 ctgtacaagt tcggcattga tctgagcggc ctgaccctgg gtaccagtgc tggaggtagc 
     1381 tccccgaaac gccctaagct gctgcgtgaa tattacgatt ggctggttga taacagcttt 
     1441 accccatatc tggtggtgga tgccacatac ctgggcgtga acgtgcccgt ggagtatgtg 
     1501 aaagacggtc agatcgtgct gaatctgtct gcaagtgcga ccggcaacct gcaactgaca 
     1561 aatgatttta tccagttcaa cgcccgcttt aagggcgtgt ctcgtgaact gtatatcccg 
     1621 atgggtgccg ctctggccat ttacgctcgc gagaacggcg atggtgtgat gttcgaacca 
     1681 gaagaaatct atgacgagct gaatattggt ctcgacagtg ctggaggtag tgctggaggt 
     1741 tcagctggtg gactcgagga tgacgggccg gacgtccgcg gaggatctgg ggacatctta 
     1801 ctggtccatg ctactgagac tgacaggaaa gatttggtgt tgtactgcga ggcattcctg 
     1861 accacctaca ggaccttcat ctccccagag gagctcatca agaagctgca gtacagatat 
     1921 gagaaattct ctccctttgc cgacacattc aagaagcgcg tcagcaagaa cacgttcttc 
     1981 gtgctggtac gggtggtgga tgagctctgc ctggtggagt tgacagaaga gatcctgaag 
     2041 ctgctgatgg aactggtctt ccgcctggtg tgcaatgggg agctgagcct ggcccgtgtg 
     2101 ctccggaaga acatcctgga caaggtggac cagaagaagc tactcaggtg tgccacctcc 
     2161 agccagcccc tggcagcccg gggggtagca gccaggccgg ggaccttgca cgactttcac 
     2221 agccatgaga tagcggagca gctaacgctg ctggatgctg agctcttcta taaaatagag 
     2281 attcctgagg ttttgctttg ggcaaaagag cagaatgagg agaagagccc caacttgacc 
     2341 cagttcacgg agcacttcaa caacatgtcc tactgggtcc ggtccataat catgttacag 
     2401 gaaaaggccc aggacaggga acggctgctc ttgaagttca tcaagatcat gaagcacttg 
     2461 cggaagctga ataacttcaa ctcctacttg gccatcctct ctgccctgga ctcggcgccc 
     2521 atccgcaggc tggagtggca gaagcagact tcagagggcc tggccgagta ctgcacactg 
     2581 atcgacagct cgtcctcctt ccgagcctac cgggccgccc tctcggaggt ggaaccgccg 
     2641 tgcatcccgt acctggggct gatcctgcag gacctgacct tcgttcacct gggaaaccca 
     2701 gactacatcg acgggaaagt gaacttctcc aagcggtggc agcagttcaa catcctcgac 
     2761 agcatgcgct gcttccagca ggcgcactat gacatgcgga ggaacgacga cattataaac 
     2821 ttcttcaatg acttcagtga ccacctggct gaggaggccc tatgggaact gtctctgaaa 
     2881 attaaaccca ggaacataac aaggagaaaa acagaccggg aagagaagac cccgcgggga 
     2941 agcggagcta ctaacttcag cctgctgaag caggctggag acgtggagga gaaccctgga 
     3001 cctgcaccgg gatccgggga gtttctggca accacactgg aacggatcga gaaaaatttc 
     3061 gtgattactg atccgagact gcctgacaac ccaatcattt ttgcgagcga ttccttcctg 
     3121 cagctgacag aatattctcg ggaagagatc ctggggcgca attgccgttt tctgcaggga 
     3181 cccgagacag accgtgccac tgttcggaaa atcagagatg ctattgacaa ccagactgaa 
     3241 gtgaccgttc agctgatcaa ttataccaag agcggcaaga agttctggaa cgtgttccac 
     3301 ctgcagccga tgcgcgatta taagggcgac gtccagtact tcattggcgt gcagctggat 
     3361 ggcaccgaac gtcttcatgg cgccgctgag cgtgaggcgg tctgcctgat caaaaagaca 
     3421 gcctttcaga ttgctgaggc agcgaacgac gaaaattact ttggaagcgg gagtgggagc 
     3481 aaaaagaaga aaaagaagtc aaagacaaag tgtgtaatta tgtaggcggc cgcgactcta 
     3541 gatcataatc agccatacca catttgtaga ggttttactt gctttaaaaa acctcccaca 
     3601 cctccccctg aacctgaaac ataaaatgaa tgcaattgtt gttgttaact tgtttattgc 
     3661 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt 
     3721 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttaag gcgtaaattg 
     3781 taagcgttaa tattttgtta aaattcgcgt taaatttttg ttaaatcagc tcatttttta 
     3841 accaataggc cgaaatcggc aaaatccctt ataaatcaaa agaatagacc gagatagggt 
     3901 tgagtgttgt tccagtttgg aacaagagtc cactattaaa gaacgtggac tccaacgtca 
     3961 aagggcgaaa aaccgtctat cagggcgatg gcccactacg tgaaccatca ccctaatcaa 
     4021 gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg agcccccgat 
     4081 ttagagcttg acggggaaag ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag 
     4141 gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc accacacccg 
     4201 ccgcgcttaa tgcgccgcta cagggcgcgt caggtggcac ttttcgggga aatgtgcgcg 
     4261 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat 
     4321 aaccctgata aatgcttcaa taatattgaa aaaggaagag tcctgaggcg gaaagaacca 
     4381 gctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag 
     4441 tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc 
     4501 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct 
     4561 aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg 
     4621 actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa 
     4681 gtagtgagga ggcttttttg gaggcctagg cttttgcaaa gatcgatcaa gagacaggat 
     4741 gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc aggttctccg gccgcttggg 
     4801 tggagaggct attcggctat gactgggcac aacagacaat cggctgctct gatgccgccg 
     4861 tgttccggct gtcagcgcag gggcgcccgg ttctttttgt caagaccgac ctgtccggtg 
     4921 ccctgaatga actgcaagac gaggcagcgc ggctatcgtg gctggccacg acgggcgttc 
     4981 cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag ggactggctg ctattgggcg 
     5041 aagtgccggg gcaggatctc ctgtcatctc accttgctcc tgccgagaaa gtatccatca 
     5101 tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc tacctgccca ttcgaccacc 
     5161 aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga agccggtctt gtcgatcagg 
     5221 atgatctgga cgaagagcat caggggctcg cgccagccga actgttcgcc aggctcaagg 
     5281 cgagcatgcc cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata 
     5341 tcatggtgga aaatggccgc ttttctggat tcatcgactg tggccggctg ggtgtggcgg 
     5401 accgctatca ggacatagcg ttggctaccc gtgatattgc tgaagagctt ggcggcgaat 
     5461 gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag cgcatcgcct 
     5521 tctatcgcct tcttgacgag ttcttctgag cgggactctg gggttcgaaa tgaccgacca 
     5581 agcgacgccc aacctgccat cacgagattt cgattccacc gccgccttct atgaaaggtt 
     5641 gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg gggatctcat 
     5701 gctggagttc ttcgcccacc ctagggggag gctaactgaa acacggaagg agacaatacc 
     5761 ggaaggaacc cgcgctatga cggcaataaa aagacagaat aaaacgcacg gtgttgggtc 
     5821 gtttgttcat aaacgcgggg ttcggtccca gggctggcac tctgtcgata ccccaccgag 
     5881 accccattgg ggccaatacg cccgcgtttc ttccttttcc ccaccccacc ccccaagttc 
     5941 gggtgaaggc ccagggctcg cagccaacgt cggggcggca ggccctgcca tagcctcagg 
     6001 ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt 
     6061 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg 
     6121 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt 
     6181 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca 
     6241 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac 
     6301 tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac 
     6361 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct 
     6421 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg 
     6481 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca 
     6541 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt 
     6601 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta 
     6661 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc 
     6721 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc 
     6781 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa 
     6841 ccgtattacc gccatgcat
//