LOCUS       pHI399_mCherry-N        6340 bp    DNA     circular     09-Jul-2021
SOURCE
  ORGANISM
COMMENT     This file was created by DNADynamo
COMMENT     www.bluetractorsoftware.co.uk
FEATURES             Location/Qualifiers
     CDS             1750..2412
                     /label="4025..4687"
                     /note="LARG-DH"
     misc_feature    1369..1701
                     /label="2477..2809"
                     /note="sspB"
     CDS             2497..3006
                     /label="4325..4834"
                     /note="iLIDcaax"
     CDS             1321..1350
                     /label="1744..1773"
                     /note="NES"
     CDS             613..1320
                     /label="613..1320"
                     /note="mCherry"
     CDS             2419..2484
                     /label="P2A"
                     /note="P2A"
     misc_feature    4236..5030
                     /label="CDS neomycin phosphotransferase"
                     /note="neomycin phosphotransferase"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg 
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac 
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg 
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg 
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt 
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta 
      601 ccggtcgcca ccatggtgag caagggcgag gaggataaca tggccatcat caaggagttc 
      661 atgcgcttca aggtgcacat ggagggctcc gtgaacggcc acgagttcga gatcgagggc 
      721 gagggcgagg gccgccccta cgagggcacc cagaccgcca agctgaaggt gaccaagggt 
      781 ggccccctgc ccttcgcctg ggacatcctg tcccctcagt tcatgtacgg ctccaaggcc 
      841 tacgtgaagc accccgccga catccccgac tacttgaagc tgtccttccc cgagggcttc 
      901 aagtgggagc gcgtgatgaa cttcgaggac ggcggcgtgg tgaccgtgac ccaggactcc 
      961 tccctgcagg acggcgagtt catctacaag gtgaagctgc gcggcaccaa cttcccctcc 
     1021 gacggccccg taatgcagaa gaagaccatg ggctgggagg cctcctccga gcggatgtac 
     1081 cccgaggacg gcgccctgaa gggcgagatc aagcagaggc tgaagctgaa ggacggcggc 
     1141 cactacgacg ctgaggtcaa gaccacctac aaggccaaga agcccgtgca gctgcccggc 
     1201 gcctacaacg tcaacatcaa gttggacatc acctcccaca acgaggacta caccatcgtg 
     1261 gaacagtacg aacgcgccga gggccgccac tccaccggcg gcatggacga gctgtacaag 
     1321 ttcggcattg atctgagcgg cctgaccctg ggtaccagtg ctggaggtag ctccccgaaa 
     1381 cgccctaagc tgctgcgtga atattacgat tggctggttg ataacagctt taccccatat 
     1441 ctggtggtgg atgccacata cctgggcgtg aacgtgcccg tggagtatgt gaaagacggt 
     1501 cagatcgtgc tgaatctgtc tgcaagtgcg accggcaacc tgcaactgac aaatgatttt 
     1561 atccagttca acgcccgctt taagggcgtg tctcgtgaac tgtatatccc gatgggtgcc 
     1621 gctctggcca tttacgctcg cgagaacggc gatggtgtga tgttcgaacc agaagaaatc 
     1681 tatgacgagc tgaatattgg tctcgacagt gctggaggta gtgctggagg ttcagctggt 
     1741 ggactcgagc cacccaactg gcagcagctt gttagtcgag aagtgttact gggactaaaa 
     1801 ccttgtgaaa tcaaaagaca ggaagtgatt aatgaattgt tctacactga aagagctcat 
     1861 gttcgaacac tgaaggttct tgatcaagtg ttctatcagc gagtatccag agaaggaatt 
     1921 ctgtcaccct cagagctacg gaaaattttt tcaaacttgg aagatattct tcaacttcat 
     1981 attggattga atgaacaaat gaaggctgtt cgaaagagaa atgagacctc tgttatcgat 
     2041 cagattgggg aagatttgct gacatggttc agcggaccag gagaggagaa attgaaacat 
     2101 gctgctgcta ccttttgcag taaccaacct ttcgccctgg aaatgatcaa atctcgtcag 
     2161 aaaaaggatt ctcgatttca gacttttgtg caagatgctg aaagtaatcc actgtgtcgt 
     2221 cgtcttcaac tgaaggatat tattcccact caaatgcaaa ggcttactaa gtacccactt 
     2281 ctgttggata atattgccaa atacacagaa tggccaacag aaagggagaa ggtgaagaaa 
     2341 gctgcagatc actgtcgtca gatcttaaat tatgtaaatc aggctgtcaa ggaggcagaa 
     2401 aacaagcagc gcccgcgggg aagcggagct actaacttca gcctgctgaa gcaggctgga 
     2461 gacgtggagg agaaccctgg acctgcaccg ggatccgggg agtttctggc aaccacactg 
     2521 gaacggatcg agaaaaattt cgtgattact gatccgagac tgcctgacaa cccaatcatt 
     2581 tttgcgagcg attccttcct gcagctgaca gaatattctc gggaagagat cctggggcgc 
     2641 aattgccgtt ttctgcaggg acccgagaca gaccgtgcca ctgttcggaa aatcagagat 
     2701 gctattgaca accagactga agtgaccgtt cagctgatca attataccaa gagcggcaag 
     2761 aagttctgga acgtgttcca cctgcagccg atgcgcgatt ataagggcga cgtccagtac 
     2821 ttcattggcg tgcagctgga tggcaccgaa cgtcttcatg gcgccgctga gcgtgaggcg 
     2881 gtctgcctga tcaaaaagac agcctttcag attgctgagg cagcgaacga cgaaaattac 
     2941 tttggaagcg ggagtgggag caaaaagaag aaaaagaagt caaagacaaa gtgtgtaatt 
     3001 atgtaggcgg ccgcgactct agatcataat cagccatacc acatttgtag aggttttact 
     3061 tgctttaaaa aacctcccac acctccccct gaacctgaaa cataaaatga atgcaattgt 
     3121 tgttgttaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa 
     3181 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa 
     3241 tgtatcttaa ggcgtaaatt gtaagcgtta atattttgtt aaaattcgcg ttaaattttt 
     3301 gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa 
     3361 aagaatagac cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa 
     3421 agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac 
     3481 gtgaaccatc accctaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga 
     3541 accctaaagg gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa 
     3601 aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc 
     3661 tgcgcgtaac caccacaccc gccgcgctta atgcgccgct acagggcgcg tcaggtggca 
     3721 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata 
     3781 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga 
     3841 gtcctgaggc ggaaagaacc agctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc 
     3901 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg 
     3961 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc 
     4021 agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc 
     4081 ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc 
     4141 ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa 
     4201 agatcgatca agagacagga tgaggatcgt ttcgcatgat tgaacaagat ggattgcacg 
     4261 caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca caacagacaa 
     4321 tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg gttctttttg 
     4381 tcaagaccga cctgtccggt gccctgaatg aactgcaaga cgaggcagcg cggctatcgt 
     4441 ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa 
     4501 gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatct caccttgctc 
     4561 ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg cttgatccgg 
     4621 ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt actcggatgg 
     4681 aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc gcgccagccg 
     4741 aactgttcgc caggctcaag gcgagcatgc ccgacggcga ggatctcgtc gtgacccatg 
     4801 gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga ttcatcgact 
     4861 gtggccggct gggtgtggcg gaccgctatc aggacatagc gttggctacc cgtgatattg 
     4921 ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt gctttacggt atcgccgctc 
     4981 ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga gcgggactct 
     5041 ggggttcgaa atgaccgacc aagcgacgcc caacctgcca tcacgagatt tcgattccac 
     5101 cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg gctggatgat 
     5161 cctccagcgc ggggatctca tgctggagtt cttcgcccac cctaggggga ggctaactga 
     5221 aacacggaag gagacaatac cggaaggaac ccgcgctatg acggcaataa aaagacagaa 
     5281 taaaacgcac ggtgttgggt cgtttgttca taaacgcggg gttcggtccc agggctggca 
     5341 ctctgtcgat accccaccga gaccccattg gggccaatac gcccgcgttt cttccttttc 
     5401 cccaccccac cccccaagtt cgggtgaagg cccagggctc gcagccaacg tcggggcggc 
     5461 aggccctgcc atagcctcag gttactcata tatactttag attgatttaa aacttcattt 
     5521 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta 
     5581 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg 
     5641 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc 
     5701 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag 
     5761 cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa 
     5821 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc 
     5881 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc 
     5941 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta 
     6001 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag 
     6061 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct 
     6121 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga 
     6181 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc 
     6241 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt 
     6301 atcccctgat tctgtggata accgtattac cgccatgcat 
//