LOCUS       pHI410_mCherry-N        6850 bp    DNA     circular     09-Jul-2021
SOURCE
  ORGANISM
COMMENT     This file was created by DNADynamo
COMMENT     www.bluetractorsoftware.co.uk
FEATURES             Location/Qualifiers
     misc_feature    1369..1701
                     /label="2477..2809"
                     /note="sspB"
     CDS             3007..3516
                     /label="4325..4834"
                     /note="iLIDcaax"
     CDS             1321..1350
                     /label="1744..1773"
                     /note="NES"
     CDS             1750..2922
                     /label="4025..4687*1"
                     /note="C3Gcat"
     CDS             613..1320
                     /label="613..1320"
                     /note="mCherry"
     CDS             2929..2994
                     /label="P2A"
                     /note="P2A"
     misc_feature    4746..5540
                     /label="CDS neomycin phosphotransferase"
                     /note="neomycin phosphotransferase"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg 
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac 
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg 
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg 
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt 
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta 
      601 ccggtcgcca ccatggtgag caagggcgag gaggataaca tggccatcat caaggagttc 
      661 atgcgcttca aggtgcacat ggagggctcc gtgaacggcc acgagttcga gatcgagggc 
      721 gagggcgagg gccgccccta cgagggcacc cagaccgcca agctgaaggt gaccaagggt 
      781 ggccccctgc ccttcgcctg ggacatcctg tcccctcagt tcatgtacgg ctccaaggcc 
      841 tacgtgaagc accccgccga catccccgac tacttgaagc tgtccttccc cgagggcttc 
      901 aagtgggagc gcgtgatgaa cttcgaggac ggcggcgtgg tgaccgtgac ccaggactcc 
      961 tccctgcagg acggcgagtt catctacaag gtgaagctgc gcggcaccaa cttcccctcc 
     1021 gacggccccg taatgcagaa gaagaccatg ggctgggagg cctcctccga gcggatgtac 
     1081 cccgaggacg gcgccctgaa gggcgagatc aagcagaggc tgaagctgaa ggacggcggc 
     1141 cactacgacg ctgaggtcaa gaccacctac aaggccaaga agcccgtgca gctgcccggc 
     1201 gcctacaacg tcaacatcaa gttggacatc acctcccaca acgaggacta caccatcgtg 
     1261 gaacagtacg aacgcgccga gggccgccac tccaccggcg gcatggacga gctgtacaag 
     1321 ttcggcattg atctgagcgg cctgaccctg ggtaccagtg ctggaggtag ctccccgaaa 
     1381 cgccctaagc tgctgcgtga atattacgat tggctggttg ataacagctt taccccatat 
     1441 ctggtggtgg atgccacata cctgggcgtg aacgtgcccg tggagtatgt gaaagacggt 
     1501 cagatcgtgc tgaatctgtc tgcaagtgcg accggcaacc tgcaactgac aaatgatttt 
     1561 atccagttca acgcccgctt taagggcgtg tctcgtgaac tgtatatccc gatgggtgcc 
     1621 gctctggcca tttacgctcg cgagaacggc gatggtgtga tgttcgaacc agaagaaatc 
     1681 tatgacgagc tgaatattgg tctcgacagt gctggaggta gtgctggagg ttcagctggt 
     1741 ggactcgagg atgacgggcc ggacgtccgc ggaggatctg gggacatctt actggtccat 
     1801 gctactgaga ctgacaggaa agatttggtg ttgtactgcg aggcattcct gaccacctac 
     1861 aggaccttca tctccccaga ggagctcatc aagaagctgc agtacagata tgagaaattc 
     1921 tctccctttg ccgacacatt caagaagcgc gtcagcaaga acacgttctt cgtgctggta 
     1981 cgggtggtgg atgagctctg cctggtggag ttgacagaag agatcctgaa gctgctgatg 
     2041 gaactggtct tccgcctggt gtgcaatggg gagctgagcc tggcccgtgt gctccggaag 
     2101 aacatcctgg acaaggtgga ccagaagaag ctactcaggt gtgccacctc cagccagccc 
     2161 ctggcagccc ggggggtagc agccaggccg gggaccttgc acgactttca cagccatgag 
     2221 atagcggagc agctaacgct gctggatgct gagctcttct ataaaataga gattcctgag 
     2281 gttttgcttt gggcaaaaga gcagaatgag gagaagagcc ccaacttgac ccagttcacg 
     2341 gagcacttca acaacatgtc ctactgggtc cggtccataa tcatgttaca ggaaaaggcc 
     2401 caggacaggg aacggctgct cttgaagttc atcaagatca tgaagcactt gcggaagctg 
     2461 aataacttca actcctactt ggccatcctc tctgccctgg actcggcgcc catccgcagg 
     2521 ctggagtggc agaagcagac ttcagagggc ctggccgagt actgcacact gatcgacagc 
     2581 tcgtcctcct tccgagccta ccgggccgcc ctctcggagg tggaaccgcc gtgcatcccg 
     2641 tacctggggc tgatcctgca ggacctgacc ttcgttcacc tgggaaaccc agactacatc 
     2701 gacgggaaag tgaacttctc caagcggtgg cagcagttca acatcctcga cagcatgcgc 
     2761 tgcttccagc aggcgcacta tgacatgcgg aggaacgacg acattataaa cttcttcaat 
     2821 gacttcagtg accacctggc tgaggaggcc ctatgggaac tgtctctgaa aattaaaccc 
     2881 aggaacataa caaggagaaa aacagaccgg gaagagaaga ccccgcgggg aagcggagct 
     2941 actaacttca gcctgctgaa gcaggctgga gacgtggagg agaaccctgg acctgcaccg 
     3001 ggatccgggg agtttctggc aaccacactg gaacggatcg agaaaaattt cgtgattact 
     3061 gatccgagac tgcctgacaa cccaatcatt tttgcgagcg attccttcct gcagctgaca 
     3121 gaatattctc gggaagagat cctggggcgc aattgccgtt ttctgcaggg acccgagaca 
     3181 gaccgtgcca ctgttcggaa aatcagagat gctattgaca accagactga agtgaccgtt 
     3241 cagctgatca attataccaa gagcggcaag aagttctgga acgtgttcca cctgcagccg 
     3301 atgcgcgatt ataagggcga cgtccagtac ttcattggcg tgcagctgga tggcaccgaa 
     3361 cgtcttcatg gcgccgctga gcgtgaggcg gtctgcctga tcaaaaagac agcctttcag 
     3421 attgctgagg cagcgaacga cgaaaattac tttggaagcg ggagtgggag caaaaagaag 
     3481 aaaaagaagt caaagacaaa gtgtgtaatt atgtaggcgg ccgcgactct agatcataat 
     3541 cagccatacc acatttgtag aggttttact tgctttaaaa aacctcccac acctccccct 
     3601 gaacctgaaa cataaaatga atgcaattgt tgttgttaac ttgtttattg cagcttataa 
     3661 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca 
     3721 ttctagttgt ggtttgtcca aactcatcaa tgtatcttaa ggcgtaaatt gtaagcgtta 
     3781 atattttgtt aaaattcgcg ttaaattttt gttaaatcag ctcatttttt aaccaatagg 
     3841 ccgaaatcgg caaaatccct tataaatcaa aagaatagac cgagataggg ttgagtgttg 
     3901 ttccagtttg gaacaagagt ccactattaa agaacgtgga ctccaacgtc aaagggcgaa 
     3961 aaaccgtcta tcagggcgat ggcccactac gtgaaccatc accctaatca agttttttgg 
     4021 ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg gagcccccga tttagagctt 
     4081 gacggggaaa gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg 
     4141 ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta 
     4201 atgcgccgct acagggcgcg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta 
     4261 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat 
     4321 aaatgcttca ataatattga aaaaggaaga gtcctgaggc ggaaagaacc agctgtggaa 
     4381 tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag 
     4441 catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag 
     4501 aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc 
     4561 catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt 
     4621 ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg 
     4681 aggctttttt ggaggcctag gcttttgcaa agatcgatca agagacagga tgaggatcgt 
     4741 ttcgcatgat tgaacaagat ggattgcacg caggttctcc ggccgcttgg gtggagaggc 
     4801 tattcggcta tgactgggca caacagacaa tcggctgctc tgatgccgcc gtgttccggc 
     4861 tgtcagcgca ggggcgcccg gttctttttg tcaagaccga cctgtccggt gccctgaatg 
     4921 aactgcaaga cgaggcagcg cggctatcgt ggctggccac gacgggcgtt ccttgcgcag 
     4981 ctgtgctcga cgttgtcact gaagcgggaa gggactggct gctattgggc gaagtgccgg 
     5041 ggcaggatct cctgtcatct caccttgctc ctgccgagaa agtatccatc atggctgatg 
     5101 caatgcggcg gctgcatacg cttgatccgg ctacctgccc attcgaccac caagcgaaac 
     5161 atcgcatcga gcgagcacgt actcggatgg aagccggtct tgtcgatcag gatgatctgg 
     5221 acgaagagca tcaggggctc gcgccagccg aactgttcgc caggctcaag gcgagcatgc 
     5281 ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat atcatggtgg 
     5341 aaaatggccg cttttctgga ttcatcgact gtggccggct gggtgtggcg gaccgctatc 
     5401 aggacatagc gttggctacc cgtgatattg ctgaagagct tggcggcgaa tgggctgacc 
     5461 gcttcctcgt gctttacggt atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc 
     5521 ttcttgacga gttcttctga gcgggactct ggggttcgaa atgaccgacc aagcgacgcc 
     5581 caacctgcca tcacgagatt tcgattccac cgccgccttc tatgaaaggt tgggcttcgg 
     5641 aatcgttttc cgggacgccg gctggatgat cctccagcgc ggggatctca tgctggagtt 
     5701 cttcgcccac cctaggggga ggctaactga aacacggaag gagacaatac cggaaggaac 
     5761 ccgcgctatg acggcaataa aaagacagaa taaaacgcac ggtgttgggt cgtttgttca 
     5821 taaacgcggg gttcggtccc agggctggca ctctgtcgat accccaccga gaccccattg 
     5881 gggccaatac gcccgcgttt cttccttttc cccaccccac cccccaagtt cgggtgaagg 
     5941 cccagggctc gcagccaacg tcggggcggc aggccctgcc atagcctcag gttactcata 
     6001 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct 
     6061 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga 
     6121 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg 
     6181 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc 
     6241 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct 
     6301 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc 
     6361 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt 
     6421 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg 
     6481 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct 
     6541 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag 
     6601 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag 
     6661 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg 
     6721 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg 
     6781 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac 
     6841 cgccatgcat 
//