LOCUS       pHI324_mVenus-NE        7240 bp    DNA     circular     30-Jan-2021
SOURCE
  ORGANISM
COMMENT     This file was created by DNADynamo
COMMENT     www.bluetractorsoftware.co.uk
FEATURES             Location/Qualifiers
     CDS             1759..3312
                     /label="RGL2_GEF"
                     /note="RGL2_GEF"
     CDS             1378..1710
                     /label="2477..2809"
                     /note="sspB"
     CDS	     3397..3906
                     /label="4325..4834"
                     /note="iLIDcaax"
     CDS             1330..1359
                     /label="1744..1773"
                     /note="NES"
     CDS             3319..3384
                     /label="P2A"
                     /note="P2A"
     CDS	     5136..5930
                     /label="CDS neomycin phosphotransferase"
                     /note="neomycin phosphotransferase"
     misc_feature    613..1329
                     /label="CDS mVenus"
                     /note="mVenus"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg 
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac 
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg 
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg 
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt 
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta 
      601 ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg 
      661 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc 
      721 gatgccacct acggcaagct gaccctgaag ttcatcagca ccaccggcaa gctgcccgtg 
      781 ccctggccca ccctcgtgac caccctcggc tacggcctgc agtgcttcgc ccgctacccc 
      841 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag 
      901 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag 
      961 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac 
     1021 atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat caccgccgac 
     1081 aagcagaaga acggcatcaa ggcaaacttc aagatccgcc acaacatcga ggacggcggc 
     1141 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg 
     1201 cccgacaacc actacctgag ctaccagtcc aaactgagca aagaccccaa cgagaagcgc 
     1261 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag 
     1321 ctgtacaagt tcggcattga tctgagcggc ctgaccctgg gtaccagtgc tggaggtagc 
     1381 tccccgaaac gccctaagct gctgcgtgaa tattacgatt ggctggttga taacagcttt 
     1441 accccatatc tggtggtgga tgccacatac ctgggcgtga acgtgcccgt ggagtatgtg 
     1501 aaagacggtc agatcgtgct gaatctgtct gcaagtgcga ccggcaacct gcaactgaca 
     1561 aatgatttta tccagttcaa cgcccgcttt aagggcgtgt ctcgtgaact gtatatcccg 
     1621 atgggtgccg ctctggccat ttacgctcgc gagaacggcg atggtgtgat gttcgaacca 
     1681 gaagaaatct atgacgagct gaatattggt ctcgacagtg ctggaggtag tgctggaggt 
     1741 tcagctggtg gactcgagat gctcccgcgg cccctgcggc tgcttttgga cacgagcccc 
     1801 cccgggggag tcgtactgag cagcttccga agccgggacc ccgaagaggg tgggggccca 
     1861 ggtggcctgg tcgtgggcgg ggggcaggag gaagaggagg aggaagaaga agaggcccct 
     1921 gtgtccgtct gggatgagga ggaggatggt gccgtgttta ccgtcacaag ccgccaatat 
     1981 cgacctcttg atcccttggt ccctatgcct cccccacgtt cctcccgacg gctccgagct 
     2041 ggcactctgg aggccctggt cagacaccta ctggataccc ggacatcagg gactgatgtg 
     2101 agcttcatgt cagccttcct ggctacccac cgggccttca cctccacgcc tgccttgcta 
     2161 gggcttatgg ctgacaggct ggaagccctt gaatcccatc ctaccgacga actagagagg 
     2221 acaacagagg tagccatctc tgtactgtca acctggctgg cctctcaccc tgaggatttt 
     2281 ggctctgagg ccaagggtca gcttgaccgg cttgagagct tcttacttca gacagggtat 
     2341 gcagcaggga agggtgttgg ggggggcagc gctgacctca tccgcaatct ccggtcccgg 
     2401 gtggaccccc aggcccccga ccttcctaag cccctggccc tccccggcga tccccctgct 
     2461 gaccccacgg atgtcctggt gttcctcgct gaccacttgg ccgaacagct gaccctgcta 
     2521 gatgcggaac tttttctcaa tttgatcccc tctcagtgcc tgggaggcct gtggggtcac 
     2581 agagaccggc caggacattc tcacctctgc ccatctgtcc gagctactgt cacacagttt 
     2641 aacaaggtgg caggggcagt ggttagttct gtcctggggg ctacttccac tggagaggga 
     2701 cctggggagg tgaccatacg gccactccgt cccccacaga gggcccggct cctggagaag 
     2761 tggatccgcg tggcagagga gtgccggctg ctccgaaact tctcttcagt ttatgccgtg 
     2821 gtgtcagccc tgcagtccag ccccatccac aggcttcggg cagcctgggg ggaagcaacc 
     2881 agggacagcc tcagagtctt ttccagcctc tgccagattt tctccgagga ggataattat 
     2941 tcccagagtc gggagctgct cgtgcaggag gtgaagctgc agtctcctct ggagccacac 
     3001 tccaagaagg ccccgaggtc tggctcccgg ggtgggggtg tggtcccata ccttggcacc 
     3061 ttcctgaagg accttgtgat gctggatgca gcctccaagg atgagttgga gaatggatac 
     3121 atcaattttg acaagcggag gaaggagttt gcagtccttt ctgagttgcg acggctccag 
     3181 aatgaatgtc gtggctataa cctccaacct gaccatgata tccagaggtg gctacagggg 
     3241 ctccggccac tgacagaggc tcagagccat cgtgtatcct gtgaggtgga gccacctggt 
     3301 tccagtgacc ctccgcgggg aagcggagct actaacttca gcctgctgaa gcaggctgga 
     3361 gacgtggagg agaaccctgg acctgcaccg ggatccgggg agtttctggc aaccacactg 
     3421 gaacggatcg agaaaaattt cgtgattact gatccgagac tgcctgacaa cccaatcatt 
     3481 tttgcgagcg attccttcct gcagctgaca gaatattctc gggaagagat cctggggcgc 
     3541 aattgccgtt ttctgcaggg acccgagaca gaccgtgcca ctgttcggaa aatcagagat 
     3601 gctattgaca accagactga agtgaccgtt cagctgatca attataccaa gagcggcaag 
     3661 aagttctgga acgtgttcca cctgcagccg atgcgcgatt ataagggcga cgtccagtac 
     3721 ttcattggcg tgcagctgga tggcaccgaa cgtcttcatg gcgccgctga gcgtgaggcg 
     3781 gtctgcctga tcaaaaagac agcctttcag attgctgagg cagcgaacga cgaaaattac 
     3841 tttggaagcg ggagtgggag caaaaagaag aaaaagaagt caaagacaaa gtgtgtaatt 
     3901 atgtaggcgg ccgcgactct agatcataat cagccatacc acatttgtag aggttttact 
     3961 tgctttaaaa aacctcccac acctccccct gaacctgaaa cataaaatga atgcaattgt 
     4021 tgttgttaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa 
     4081 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa 
     4141 tgtatcttaa ggcgtaaatt gtaagcgtta atattttgtt aaaattcgcg ttaaattttt 
     4201 gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa 
     4261 aagaatagac cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa 
     4321 agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac 
     4381 gtgaaccatc accctaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga 
     4441 accctaaagg gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa 
     4501 aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc 
     4561 tgcgcgtaac caccacaccc gccgcgctta atgcgccgct acagggcgcg tcaggtggca 
     4621 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata 
     4681 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga 
     4741 gtcctgaggc ggaaagaacc agctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc 
     4801 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg 
     4861 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc 
     4921 agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc 
     4981 ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc 
     5041 ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa 
     5101 agatcgatca agagacagga tgaggatcgt ttcgcatgat tgaacaagat ggattgcacg 
     5161 caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca caacagacaa 
     5221 tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg gttctttttg 
     5281 tcaagaccga cctgtccggt gccctgaatg aactgcaaga cgaggcagcg cggctatcgt 
     5341 ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa 
     5401 gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatct caccttgctc 
     5461 ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg cttgatccgg 
     5521 ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt actcggatgg 
     5581 aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc gcgccagccg 
     5641 aactgttcgc caggctcaag gcgagcatgc ccgacggcga ggatctcgtc gtgacccatg 
     5701 gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga ttcatcgact 
     5761 gtggccggct gggtgtggcg gaccgctatc aggacatagc gttggctacc cgtgatattg 
     5821 ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt gctttacggt atcgccgctc 
     5881 ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga gcgggactct 
     5941 ggggttcgaa atgaccgacc aagcgacgcc caacctgcca tcacgagatt tcgattccac 
     6001 cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg gctggatgat 
     6061 cctccagcgc ggggatctca tgctggagtt cttcgcccac cctaggggga ggctaactga 
     6121 aacacggaag gagacaatac cggaaggaac ccgcgctatg acggcaataa aaagacagaa 
     6181 taaaacgcac ggtgttgggt cgtttgttca taaacgcggg gttcggtccc agggctggca 
     6241 ctctgtcgat accccaccga gaccccattg gggccaatac gcccgcgttt cttccttttc 
     6301 cccaccccac cccccaagtt cgggtgaagg cccagggctc gcagccaacg tcggggcggc 
     6361 aggccctgcc atagcctcag gttactcata tatactttag attgatttaa aacttcattt 
     6421 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta 
     6481 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg 
     6541 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc 
     6601 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag 
     6661 cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa 
     6721 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc 
     6781 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc 
     6841 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta 
     6901 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag 
     6961 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct 
     7021 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga 
     7081 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc 
     7141 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt 
     7201 atcccctgat tctgtggata accgtattac cgccatgcat 
//