LOCUS       pHI411_mCherry-N        7231 bp    DNA     circular     30-Jan-2021
SOURCE
  ORGANISM
COMMENT     This file was created by DNADynamo
COMMENT     www.bluetractorsoftware.co.uk
FEATURES             Location/Qualifiers
     CDS             3388..3897
                     /label="iLIDcaax"
                     /note="iLIDcaax"
     CDS             1750..3303
                     /label="RGL2_GEF"
                     /note="RGL2_GEF"
     CDS             613..1320
                     /label="mCherry"
                     /note="mCherry"
     CDS             1369..1701
                     /label="SspB"
                     /note="SspB"
     CDS             1321..1350
                     /label="NES"
                     /note="NES"
     CDS             3310..3375
                     /label="P2A"
                     /note="P2A"
     misc_feature    5127..5921
                     /label="CDS neomycin phosphotransferase"
                     /note="neomycin phosphotransferase"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg 
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac 
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg 
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg 
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt 
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta 
      601 ccggtcgcca ccatggtgag caagggcgag gaggataaca tggccatcat caaggagttc 
      661 atgcgcttca aggtgcacat ggagggctcc gtgaacggcc acgagttcga gatcgagggc 
      721 gagggcgagg gccgccccta cgagggcacc cagaccgcca agctgaaggt gaccaagggt 
      781 ggccccctgc ccttcgcctg ggacatcctg tcccctcagt tcatgtacgg ctccaaggcc 
      841 tacgtgaagc accccgccga catccccgac tacttgaagc tgtccttccc cgagggcttc 
      901 aagtgggagc gcgtgatgaa cttcgaggac ggcggcgtgg tgaccgtgac ccaggactcc 
      961 tccctgcagg acggcgagtt catctacaag gtgaagctgc gcggcaccaa cttcccctcc 
     1021 gacggccccg taatgcagaa gaagaccatg ggctgggagg cctcctccga gcggatgtac 
     1081 cccgaggacg gcgccctgaa gggcgagatc aagcagaggc tgaagctgaa ggacggcggc 
     1141 cactacgacg ctgaggtcaa gaccacctac aaggccaaga agcccgtgca gctgcccggc 
     1201 gcctacaacg tcaacatcaa gttggacatc acctcccaca acgaggacta caccatcgtg 
     1261 gaacagtacg aacgcgccga gggccgccac tccaccggcg gcatggacga gctgtacaag 
     1321 ttcggcattg atctgagcgg cctgaccctg ggtaccagtg ctggaggtag ctccccgaaa 
     1381 cgccctaagc tgctgcgtga atattacgat tggctggttg ataacagctt taccccatat 
     1441 ctggtggtgg atgccacata cctgggcgtg aacgtgcccg tggagtatgt gaaagacggt 
     1501 cagatcgtgc tgaatctgtc tgcaagtgcg accggcaacc tgcaactgac aaatgatttt 
     1561 atccagttca acgcccgctt taagggcgtg tctcgtgaac tgtatatccc gatgggtgcc 
     1621 gctctggcca tttacgctcg cgagaacggc gatggtgtga tgttcgaacc agaagaaatc 
     1681 tatgacgagc tgaatattgg tctcgacagt gctggaggta gtgctggagg ttcagctggt 
     1741 ggactcgaga tgctcccgcg gcccctgcgg ctgcttttgg acacgagccc ccccggggga 
     1801 gtcgtactga gcagcttccg aagccgggac cccgaagagg gtgggggccc aggtggcctg 
     1861 gtcgtgggcg gggggcagga ggaagaggag gaggaagaag aagaggcccc tgtgtccgtc 
     1921 tgggatgagg aggaggatgg tgccgtgttt accgtcacaa gccgccaata tcgacctctt 
     1981 gatcccttgg tccctatgcc tcccccacgt tcctcccgac ggctccgagc tggcactctg 
     2041 gaggccctgg tcagacacct actggatacc cggacatcag ggactgatgt gagcttcatg 
     2101 tcagccttcc tggctaccca ccgggccttc acctccacgc ctgccttgct agggcttatg 
     2161 gctgacaggc tggaagccct tgaatcccat cctaccgacg aactagagag gacaacagag 
     2221 gtagccatct ctgtactgtc aacctggctg gcctctcacc ctgaggattt tggctctgag 
     2281 gccaagggtc agcttgaccg gcttgagagc ttcttacttc agacagggta tgcagcaggg 
     2341 aagggtgttg gggggggcag cgctgacctc atccgcaatc tccggtcccg ggtggacccc 
     2401 caggcccccg accttcctaa gcccctggcc ctccccggcg atccccctgc tgaccccacg 
     2461 gatgtcctgg tgttcctcgc tgaccacttg gccgaacagc tgaccctgct agatgcggaa 
     2521 ctttttctca atttgatccc ctctcagtgc ctgggaggcc tgtggggtca cagagaccgg 
     2581 ccaggacatt ctcacctctg cccatctgtc cgagctactg tcacacagtt taacaaggtg 
     2641 gcaggggcag tggttagttc tgtcctgggg gctacttcca ctggagaggg acctggggag 
     2701 gtgaccatac ggccactccg tcccccacag agggcccggc tcctggagaa gtggatccgc 
     2761 gtggcagagg agtgccggct gctccgaaac ttctcttcag tttatgccgt ggtgtcagcc 
     2821 ctgcagtcca gccccatcca caggcttcgg gcagcctggg gggaagcaac cagggacagc 
     2881 ctcagagtct tttccagcct ctgccagatt ttctccgagg aggataatta ttcccagagt 
     2941 cgggagctgc tcgtgcagga ggtgaagctg cagtctcctc tggagccaca ctccaagaag 
     3001 gccccgaggt ctggctcccg gggtgggggt gtggtcccat accttggcac cttcctgaag 
     3061 gaccttgtga tgctggatgc agcctccaag gatgagttgg agaatggata catcaatttt 
     3121 gacaagcgga ggaaggagtt tgcagtcctt tctgagttgc gacggctcca gaatgaatgt 
     3181 cgtggctata acctccaacc tgaccatgat atccagaggt ggctacaggg gctccggcca 
     3241 ctgacagagg ctcagagcca tcgtgtatcc tgtgaggtgg agccacctgg ttccagtgac 
     3301 cctccgcggg gaagcggagc tactaacttc agcctgctga agcaggctgg agacgtggag 
     3361 gagaaccctg gacctgcacc gggatccggg gagtttctgg caaccacact ggaacggatc 
     3421 gagaaaaatt tcgtgattac tgatccgaga ctgcctgaca acccaatcat ttttgcgagc 
     3481 gattccttcc tgcagctgac agaatattct cgggaagaga tcctggggcg caattgccgt 
     3541 tttctgcagg gacccgagac agaccgtgcc actgttcgga aaatcagaga tgctattgac 
     3601 aaccagactg aagtgaccgt tcagctgatc aattatacca agagcggcaa gaagttctgg 
     3661 aacgtgttcc acctgcagcc gatgcgcgat tataagggcg acgtccagta cttcattggc 
     3721 gtgcagctgg atggcaccga acgtcttcat ggcgccgctg agcgtgaggc ggtctgcctg 
     3781 atcaaaaaga cagcctttca gattgctgag gcagcgaacg acgaaaatta ctttggaagc 
     3841 gggagtggga gcaaaaagaa gaaaaagaag tcaaagacaa agtgtgtaat tatgtaggcg 
     3901 gccgcgactc tagatcataa tcagccatac cacatttgta gaggttttac ttgctttaaa 
     3961 aaacctccca cacctccccc tgaacctgaa acataaaatg aatgcaattg ttgttgttaa 
     4021 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa 
     4081 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta 
     4141 aggcgtaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca 
     4201 gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga 
     4261 ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg 
     4321 actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat 
     4381 caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag 
     4441 ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga 
     4501 agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa 
     4561 ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc gtcaggtggc acttttcggg 
     4621 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc 
     4681 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtcctgagg 
     4741 cggaaagaac cagctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc 
     4801 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc 
     4861 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat 
     4921 agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc 
     4981 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga 
     5041 gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca aagatcgatc 
     5101 aagagacagg atgaggatcg tttcgcatga ttgaacaaga tggattgcac gcaggttctc 
     5161 cggccgcttg ggtggagagg ctattcggct atgactgggc acaacagaca atcggctgct 
     5221 ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg 
     5281 acctgtccgg tgccctgaat gaactgcaag acgaggcagc gcggctatcg tggctggcca 
     5341 cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac tgaagcggga agggactggc 
     5401 tgctattggg cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct cctgccgaga 
     5461 aagtatccat catggctgat gcaatgcggc ggctgcatac gcttgatccg gctacctgcc 
     5521 cattcgacca ccaagcgaaa catcgcatcg agcgagcacg tactcggatg gaagccggtc 
     5581 ttgtcgatca ggatgatctg gacgaagagc atcaggggct cgcgccagcc gaactgttcg 
     5641 ccaggctcaa ggcgagcatg cccgacggcg aggatctcgt cgtgacccat ggcgatgcct 
     5701 gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg attcatcgac tgtggccggc 
     5761 tgggtgtggc ggaccgctat caggacatag cgttggctac ccgtgatatt gctgaagagc 
     5821 ttggcggcga atgggctgac cgcttcctcg tgctttacgg tatcgccgct cccgattcgc 
     5881 agcgcatcgc cttctatcgc cttcttgacg agttcttctg agcgggactc tggggttcga 
     5941 aatgaccgac caagcgacgc ccaacctgcc atcacgagat ttcgattcca ccgccgcctt 
     6001 ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga tcctccagcg 
     6061 cggggatctc atgctggagt tcttcgccca ccctaggggg aggctaactg aaacacggaa 
     6121 ggagacaata ccggaaggaa cccgcgctat gacggcaata aaaagacaga ataaaacgca 
     6181 cggtgttggg tcgtttgttc ataaacgcgg ggttcggtcc cagggctggc actctgtcga 
     6241 taccccaccg agaccccatt ggggccaata cgcccgcgtt tcttcctttt ccccacccca 
     6301 ccccccaagt tcgggtgaag gcccagggct cgcagccaac gtcggggcgg caggccctgc 
     6361 catagcctca ggttactcat atatacttta gattgattta aaacttcatt tttaatttaa 
     6421 aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt 
     6481 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt 
     6541 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg 
     6601 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca 
     6661 gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt 
     6721 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga 
     6781 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc 
     6841 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact 
     6901 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga 
     6961 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg 
     7021 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt 
     7081 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt 
     7141 acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga 
     7201 ttctgtggat aaccgtatta ccgccatgca t
//