LOCUS       Exported                5860 bp ds-DNA     circular SYN 09-JUL-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5860)
  AUTHORS   Jason Chin
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Jul 9, 2021 from SnapGene 5.2.5
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5860
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     complement(2395..2411)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             2756..2800
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     terminator      2852..2899
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     CDS             complement(2965..3624)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     promoter        complement(3625..3715)
                     /gene="bla"
                     /label=AmpR promoter
     promoter        3736..3764
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    3772..3788
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             5002..5046
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     terminator      5098..5145
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      complement(5202..5790)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 ggatcctcgg gagttgtcag cctgtcccgc ttataagatc atacgccgtt atacgttgtt
       61 tacgctttga ggaatcccat atgatgttca aacgcgaaga aatcatcgaa atggcgaaca
      121 aagacttcga aaaggcctgg attgaaacca aggatctgat caaggcgaag aagatcaacg
      181 agagctatcc acgtatcaaa ccggtgttcg gcaaaaccca tccggtaaac gacacgatcg
      241 agaacctgcg tcaggcctac ctgcgtatgg gttttgaaga gtatatcaac ccggtgattg
      301 tggacgaacg cgatatctac aagcagtttg gtccagaagc catggcagtg ctggaccgtt
      361 gtttctacct ggcaggttta ccacgtcctg atgtcggtct gtctgatgaa aagatcagcc
      421 agatcgagaa actggggatc aaagttagcg aacacaaaga gagcctgcag aagattctgc
      481 acggctacaa gaaaggcacc ctggatggcg atgatctggt cctggagatt agcaacgctc
      541 tggagatcag ctcggagatg gggctgaaga tcctggaaga tgtgtttccg gagttcaaag
      601 atctgacggc agtttcctct aagctgacgc tgcgtagtca tatgaccagc ggctggtttc
      661 tgaccgtgtc tgacctgatg aacaagaaac cgttgccgtt caaactgttc agcatcgatc
      721 gttgctttcg tcgtgaacag aaggaagaca aaagccatct gatgacctat cattctgcca
      781 gctgtgcaat tgcgggtgaa ggtgtggaca tcaacgacgg caaagctatt gccgaaggtc
      841 tgctgtccca gtttggcttt acgaacttca agttcatccc ggatgagaaa aagtcgaagt
      901 actacacccc agaaacccaa acggaagttt atgcgtatca tccgaaactc aaggaatggc
      961 tggaagttgc cacgtttggt gtgtattctc cagtggccct cagcaagtat ggtattgatg
     1021 tgccggttat gaacctgggt ctgggcgttg agcgcttagc gatgattagc ggtaacttcg
     1081 cagacgtccg tgaaatggta tacccccagt tctacgagca caaactgaac gaccgcaacg
     1141 ttgcgtcgat ggtaaagctg gacaaagttc ccgttatgga cgagatctac gatctgacca
     1201 aagaactgat tgaaagctgc gtcaagaaca aggatctgaa aagcccatgc gaactggcaa
     1261 ttgagaagac gttcagcttc ggtaagacca agaagaacgt gaagatcaac atcttcgaga
     1321 aggaggaagg caagaacctg ctgggtccga gcatcctgaa cgagatctac gtgtacgacg
     1381 gcaacgtgat cgggatccca gaaagctttg acggtgtgaa agaggagttc aaggacttcc
     1441 tggagaaagg caagagcgaa ggtgtggcaa ccggtatccg ctacatcgac gcgctgtgct
     1501 tcaaaatcac cagcaagctg gaggaagcgt ttgtgagcaa cacgaccgag ttcaaggtga
     1561 aagtgcctat tgtgcgtagc ctgtccgaca tcaacctgaa aatcgacgat attgccctga
     1621 aacagatcat gagcaagaac aaggtgattg acgtgcgtgg tccagtgttc ctgaacgtgg
     1681 aggtgaaaat cgagtaatgc agtttcaaac gctaaattgc ctgatgcgct acgcttatca
     1741 ggcctacatg atctctgcaa tatattgagt ttgcgtgctt ttgtaggccg gataaggcgt
     1801 tcacgccgca tccggcaaga aacagcaaac aatccaaaac gccgcgttcg gcggtcgaca
     1861 cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa
     1921 tggtgcaggg cgcttgtttc ggcgtgggta tggtggcagg ccccgtggcc gggggactgt
     1981 tgggcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg
     2041 cgacgatagt catgccccgc gcccaccgga aggagctacc ggcagcggtg cggactgttg
     2101 taactcagaa taagaaatga ggccgctcat ggcgttctgt tgcccgtctc actggtgaaa
     2161 agaaaaacaa ccctggcgcc gcttctttga gcgaacgatc aaaaataagt ggcgccccat
     2221 caaaaaaata ttctcaacat aaaaaacttt gtgtaatact tgtaacgcta gatctgccgg
     2281 ggtagtctag gggttaggca gcgacctcta aagtcgcctt acgtgggttc aaatcccacc
     2341 cccggctcca actagtatcc ttagcgaaag ctaaggattt tttttaagct tggcactggc
     2401 cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc
     2461 agcacatccc cctttcgcca gacgctctcc cttatgcgac tcctgcatta ggaagcagcc
     2521 cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagcc
     2581 cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctggcggcc
     2641 gcataatgct taagtcgaac agaaagtaat cgtattgtac acggccgcat aatcgaaatt
     2701 aaattggata tcggcaggcc acgcgatcgc tgacgtcggt accctcgagt ctggtaaaga
     2761 aaccgctgct gcgaaatttg aacgccagca catggactcg tctactagcg cagcttaatt
     2821 aacctaggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg
     2881 ggtcttgagg ggttttttgc tgaaacctca ggcatttgag aagcacacgg tcacactgct
     2941 tccggtagtc aataaaccgg taaattacgc cccgccctgc cactcatcgc agtactgttg
     3001 taattcatta agcattctgc cgacatggaa gccatcacaa acggcatgat gaacctgaat
     3061 cgccagcggc atcagcacct tgtcgccttg cgtataatat ttgcccatgg tgaaaacggg
     3121 ggcgaagaag ttgtccatat tggccacgtt taaatcaaaa ctggtgaaac tcacccaggg
     3181 attggctgag acgaaaaaca tattctcaat aaacccttta gggaaatagg ccaggttttc
     3241 accgtaacac gccacatctt gcgaatatat gtgtagaaac tgccggaaat cgtcgtggta
     3301 ttcactccag agcgatgaaa acgtttcagt ttgctcatgg aaaacggtgt aacaagggtg
     3361 aacactatcc catatcacca gctcaccgtc tttcattgcc atacggaatt ccggatgagc
     3421 attcatcagg cgggcaagaa tgtgaataaa ggccggataa aacttgtgct tatttttctt
     3481 tacggtcttt aaaaaggccg taatatccag ctgaacggtc tggttatagg tacattgagc
     3541 aactgactga aatgcctcaa aatgttcttt acgatgccat tgggatatat caacggtggt
     3601 atatccagtg atttttttct ccatctcttc ctttttcaat attattgaag catttatcag
     3661 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaatagta
     3721 ttctgaaatg agctgttgac aattaatcat cggctcgtat aatgtgtgga attgtgagcg
     3781 gataacaatt tcacaaggag gtccatgtct aaagaaaagt ttgaacgtac aaaaccgcac
     3841 gttaacgtcg gtactatcgg ccacgttgac catggtaaaa caacgctgac cgctgcaatc
     3901 actaccgtac tggctaaaac ctacggcggt gctgctcgcg cattcgacca gatcgataac
     3961 gcgccggaag aaaaagctcg tggtatcacc atcaacactt ctcgcgttga atacgacacc
     4021 ccgacccgtc actacgcaca cgtagactgc ccggggcacg ccgactatgt taaaaacatg
     4081 atcaccggtg ctgcgcagat ggacggcgcg atcctggtag ttgctgcgac tgacggcccg
     4141 atgccgcaga ctcgtgagca catcctgctg ggtcgtcagg taggcgttcc gtacatcatc
     4201 gtgttcctga acaaatgcga catggttgat gacgaagagc tgctggaact ggttgaaatg
     4261 gaagttcgtg aacttctgtc tcagtacgac ttcccgggcg acgacactcc gatcgttcgt
     4321 ggttctgctc tgaaagcgct ggaaggcgac gcagagtggg aagcgaaaat cctggaactg
     4381 gctggcttcc tggattctta cattccggaa ccagagcgtg cgattgacaa gccgttcctg
     4441 ctgccgatca acggcgtata ctccatctcc ggtcgtggta ccgttgttag cggtcgtgta
     4501 gaacgcggta tcatcaaagt tggtgaagaa gttgaaatcg ttggtatcaa agagactcag
     4561 aagtctacct gtactggcgt tgaaatgttc cgcaaactgc tggacgaagg ccgtgctggt
     4621 gagtgggtag gtgttctgct gcgtggtatc aaacgtgaag aaatcgaacg tggtcaggta
     4681 ctggctaagc cgggcaccat caagccgcac accaagttcg aatctgaagt gtacattctg
     4741 tccaaagatg aaggcggccg tcatactccg ttcttcaaag gctaccgtcc gcagttctac
     4801 ttccgtacta ctgacgtgac tggtaccatc gaactgccgg aaggcgtaga gatggtaatg
     4861 ccgggcgaca acatcaaaat ggttgttacc ctgatccacc cgatcgcgat ggacgacggt
     4921 ctgcgtttcg caatccgtga aggcggccgt accgttggcg cgggcgttgt agcaaaagtt
     4981 ctgagctaac tcgagtctgg taaagaaacc gctgctgcga aatttgaacg ccagcacatg
     5041 gactcgtcta ctagcgcagc ttaattaacc taggctgctg ccaccgctga gcaataacta
     5101 gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgcgagc aaaaggccag
     5161 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
     5221 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     5281 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     5341 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
     5401 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     5461 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     5521 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     5581 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     5641 aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
     5701 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
     5761 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
     5821 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
//
