LOCUS       Exported                5526 bp ds-DNA     circular SYN 12-JUL-2021
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5526)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Jul 12, 2021 from SnapGene 4.3.11
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5526
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        1..138
                     /label=Spn promoter
     CDS             139..1149
                     /label=SpnR
     rep_origin      1216..1889
                     /direction=RIGHT
                     /label=pUC origin
     terminator      2319..2362
                     /label=rrnB T1 terminatior
     primer_bind     2429..2444
                     /label=M13 (-20) forward primer
     misc_recomb     2461..2560
                     /label=attL1
     CDS             2579..2629
                     /codon_start=1
                     /label=N-terminal NLS
                     /note="N-terminal NLS"
                     /translation="MAPKKKRKVGIHGVPAA"
     misc_feature    2585..2605
                     /label=SV40 NLS
                     /note="SV40 NLS"
     CDS             2630..4897
                     /codon_start=1
                     /label=Cas12j2 (rice codon optimized)
                     /translation="PKPAVESEFSKVLKKHFPGERFRSSYMKRGGKILAAQGEEAVVAY
                     LQGKSEEEPPNFQPPAKCHVVTKSRDFAEWPIMKASEAIQRYIYALSTTERAACKPGKS
                     SESHAAWFAATGVSNHGYSHVQGLNLIFDHTLGRYDGVLKKVQLRNEKARARLESINAS
                     RADEGLPEIKAEEEEVATNETGHLLQPPGINPSFYVYQTISPQAYRPRDEIVLPPEYAG
                     YVRDPNAPIPLGVVRNRCDIQKGCPGYIPEWQREAGTAISPKTGKAVTVPGLSPKKNKR
                     MRRYWRSEKEKAQDALLVTVRIGTDWVVIDVRGLLRNARWRTIAPKDISLNALLDLFTG
                     DPVIDVRRNIVTFTYTLDACGTYARKWTLKGKQTKATLDKLTATQTVALVAIDLGQTNP
                     ISAGISRVTQENGALQCEPLDRFTLPDDLLKDISAYRIAWDRNEEELRARSVEALPEAQ
                     QAEVRALDGVSKETARTQLCADFGLDPKRLPWDKMSSNTTFISEALLSNSVSRDQVFFT
                     PAPKKGAKKKAPVEVMRKDRTWARAYKPRLSVEAQKLKNEALWALKRTSPEYLKLSRRK
                     EELCRRSINYVIEKTRRRTQCQIVIPVIEDLNVRFFHGSGKRLPGWDNFFTAKKENRWF
                     IQGLHKAFSDLRTHRSFYVFEVRPERTSITCPKCGHCEVGNRDGEAFQCLSCGKTCNAD
                     LDVATHNLTQVALTGKTMPKREEPRDAQGTAPARKTKKASKSKAPPAEREDQTPAQEPS
                     QTS"
     misc_feature    4898..4945
                     /label=Nucleoplasmin NLS
                     /note="Nucleoplasmin NLS"
     misc_feature    4946..4948
                     /label=stop
                     /note="stop"
     misc_feature    4974..5251
                     /label=NOS term
     misc_recomb     5273..5396
                     /label=attR5
     primer_bind     5437..5453
                     /label=M13 reverse primer
     promoter        5525..5526
                     /label=Spn promoter
ORIGIN
        1 tggaccagcc aggacagaaa tgcctcgact tcgctgctac ccaaggttgc cgggtgacgc
       61 acaccgtgga aacggatgaa ggcacgaacc cagtggacat aagcctgttc ggttcgtaag
      121 ctgtaatgca agtagcgtat gcgctcacgc aactggtcca gaaccttgac cgaacgcagc
      181 ggtggtaacg gcgcagtggc ggttttcatg gcttgttatg actgtttttt tggggtacag
      241 tctatgcctc gggcatccaa gcagcaagcg cgttacgccg tgggtcgatg tttgatgtta
      301 tggagcagca acgatgttac gcagcagggc agtcgcccta aaacaaagtt aaacatcatg
      361 agggaagcgg tgatcgccga agtatcgact caactatcag aggtagttgg cgtcatcgag
      421 cgccatctcg aaccgacgtt gctggccgta catttgtacg gctccgcagt ggatggcggc
      481 ctgaagccac acagtgatat tgatttgctg gttacggtga ccgtaaggct tgatgaaaca
      541 acgcggcgag ctttgatcaa cgaccttttg gaaacttcgg cttcccctgg agagagcgag
      601 attctccgcg ctgtagaagt caccattgtt gtgcacgacg acatcattcc gtggcgttat
      661 ccagctaagc gcgaactgca atttggagaa tggcagcgca atgacattct tgcaggtatc
      721 ttcgagccag ccacgatcga cattgatctg gctatcttgc tgacaaaagc aagagaacat
      781 agcgttgcct tggtaggtcc agcggcggag gaactctttg atccggttcc tgaacaggat
      841 ctatttgagg cgctaaatga aaccttaacg ctatggaact cgccgcccga ctgggctggc
      901 gatgagcgaa atgtagtgct tacgttgtcc cgcatttggt acagcgcagt aaccggcaaa
      961 atcgcgccga aggatgtcgc tgccgactgg gcaatggagc gcctgccggc ccagtatcag
     1021 cccgtcatac ttgaagctag acaggcttat cttggacaag aagaagatcg cttggcctcg
     1081 cgcgcagatc agttggaaga atttgtccac tacgtgaaag gcgagatcac caaggtagtc
     1141 ggcaaataac cctcgagcca cccatgacca aaatccctta acgtgagtta cgcgtcgttc
     1201 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     1261 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     1321 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     1381 aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     1441 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     1501 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     1561 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     1621 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     1681 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     1741 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     1801 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     1861 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
     1921 gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag
     1981 cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc
     2041 gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc
     2101 agtgagcgca acgcaattaa tacgcgtacc gcgagccagg aagagtttgt agaaacgcaa
     2161 aaaggccatc cgtcaggatg gccttctgct tagtttgatg cctggcagtt tatggcgggc
     2221 gtcctgcccg ccaccctccg ggccgttgct tcacaacgtt caaatccgct cccggcggat
     2281 ttgtcctact caggagagcg ttcaccgaca aacaacagat aaaacgaaag gcccagtctt
     2341 ccgactgagc ctttcgtttt atttgatgcc tggcagttcc ctactctcgc gttaacgctt
     2401 gcatggatgt tttcccagtc acgacgttgt aaaacgacgg ccagtcttaa gctcgggccc
     2461 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa
     2521 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccgaattcgc ccttcaccat
     2581 ggctcctaag aagaagcgga aggttggtat tcacggggtg cctgcggctc ctaaaccggc
     2641 tgtcgaaagc gaattctcta aagtgttgaa aaagcacttc cctggcgaga gattcagatc
     2701 atcctacatg aaaagagggg ggaagattct ggcggctcaa ggagaggaag ccgtcgtggc
     2761 atatctccag ggcaaatccg aggaggaacc accgaacttt caacctccag caaagtgcca
     2821 tgtggtcacg aagtcccggg attttgcgga gtggccaatc atgaaggcaa gtgaagccat
     2881 acagcgctat atctacgccc ttagcactac tgaaagagcg gcgtgtaagc ctggaaaatc
     2941 ctctgagtcg cacgcggcat ggtttgctgc tacaggggtg tctaatcatg gatatagcca
     3001 tgttcagggt cttaacctga tctttgacca tacactggga cgctacgatg gggtgttgaa
     3061 gaaggttcaa ctgaggaatg aaaaagcaag agcgcgcctt gagagcatca acgcgagccg
     3121 cgctgacgaa ggactcccgg aaatcaaggc agaagaagaa gaagttgcaa cgaatgagac
     3181 gggacacttg ctgcagccac ccggaataaa cccttccttt tatgtttatc agactatcag
     3241 ccctcaggct tatcgccccc gcgatgagat cgtccttccg cctgagtacg ctggctacgt
     3301 tagggaccca aacgcgccta taccgttggg cgtcgttagg aatagatgcg atatacagaa
     3361 aggctgccca gggtatatac ccgagtggca gcgcgaggct gggactgcga tatcacctaa
     3421 aaccggcaaa gccgttacgg tgccgggatt gagtcctaaa aaaaataaga gaatgaggag
     3481 gtattggcgg tcagagaagg agaaggccca agatgcactc cttgtcaccg tgaggatagg
     3541 cacggattgg gtcgtcattg acgttagagg gcttttgagg aacgcacggt ggaggactat
     3601 tgcgccgaag gacattagtc tgaacgcgct tctcgatctg ttcactggcg acccagttat
     3661 tgatgtgagg cggaacatag tcacctttac ttatactctg gacgcatgcg gaacatacgc
     3721 tcgcaagtgg accctgaaag gaaagcagac taaggccaca ctggataagt tgacagccac
     3781 gcagacagtc gctttggtgg ccatagacct tgggcaaacc aacccaatta gtgccgggat
     3841 cagcagggtt actcaggaga atggtgcact tcagtgcgag cctctcgatc gctttacact
     3901 ccccgatgat cttcttaaag acatcagtgc ctacagaatc gcctgggacc ggaacgagga
     3961 ggaactccgc gctcgcagtg ttgaagcctt gccagaagca cagcaggctg aggttagggc
     4021 actggatggc gtttctaaag agactgccag aactcaattg tgtgctgatt ttgggcttga
     4081 tcctaagaga ctcccttggg ataaaatgtc gtcgaatact accttcatct ccgaggccct
     4141 gcttagtaac agcgttagtc gcgaccaagt ctttttcaca cccgccccga aaaaaggcgc
     4201 gaaaaagaaa gctccggtgg aagttatgag gaaagaccgc acatgggcac gcgcttacaa
     4261 accgagactg tcagttgaag cgcagaaatt gaagaacgaa gcactttggg ccttgaagcg
     4321 gacatctcca gagtatctta aactctccag gagaaaggaa gaactctgta gaaggtcgat
     4381 taattacgtt atagagaaga cacgccggag gactcagtgt caaattgtta ttccggtcat
     4441 cgaagacctc aatgttcgct tctttcatgg ttctggaaag cggttgcccg gatgggacaa
     4501 tttctttaca gctaaaaaag aaaaccgctg gttcatacaa ggattgcaca aggcctttag
     4561 tgatctccgg acgcatcgct ccttttatgt cttcgaggtg agaccggaaa gaacgtccat
     4621 cacatgccca aagtgtggac attgtgaagt cgggaaccgg gacggtgagg cctttcaatg
     4681 cctttcgtgc gggaagacct gtaatgcgga tcttgatgtg gctacgcata atctcactca
     4741 ggtggctctc acaggcaaaa caatgccgaa gagagaggaa cctagggatg cacaagggac
     4801 ggccccggcc agaaaaacga agaaggctag caagtccaag gcgccgccgg cggagaggga
     4861 ggatcagact ccagcccagg aaccatccca aacaagtaag cgtcctgctg ccaccaaaaa
     4921 ggccggacag gctaagaaaa agaagtgaga cgactagtgg cggccgccga cgtccgatcg
     4981 ttcaaacatt tggcaataaa gtttcttaag attgaatcct gttgccggtc ttgcgatgat
     5041 tatcatataa tttctgttga attacgttaa gcatgtaata attaacatgt aatgcatgac
     5101 gttatttatg agatgggttt ttatgattag agtcccgcaa ttatacattt aatacgcgat
     5161 agaaaacaaa atatagcgcg caaactagga taaattatcg cgcgcggtgt catctatgtt
     5221 actagatcgg gaattgatcc cccctcgaca gcttccggaa agggcgaatt cgcaactttg
     5281 tatacaaaag ttgaacgaga aacgtaaaat gatataaata tcaatatatt aaattagatt
     5341 ttgcataaaa aacagactac ataatactgt aaaacacaac atatccagtc actatgccat
     5401 ccagctgata tcccctatag tgagtcgtat tacatggtca tagctgtttc ctggcagctc
     5461 tggcccgtgt ctcaaaatct ctgatgttac attgcacaag ataaaaatat atcatcatgc
     5521 ctcctc
//
