LOCUS       Exported                4997 bp ds-DNA     circular SYN 12-JUL-2021
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4997)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Jul 12, 2021 from SnapGene 4.3.11
            https://www.snapgene.com
COMMENT     LOCUS pYPQ104_correct_ 3623 bp ds-DNA circular 02-MAR-2014 SOURCE 
            ORGANISM COMMENT COMMENT ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     source          1..4997
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    2..25
                     /label=M13-F1
                     /note="M13-F1"
     misc_feature    37..149
                     /label=attL5
     promoter        167..2129
                     /label=Ubi1 (maize ubiquitin promoter 1)
                     /note="Ubi1 (maize ubiquitin promoter 1)"
     misc_feature    2136..2141
                     /label=1st 6nt complement
     stem_loop       2142..2178
                     /label=Hammerhead ribozyme
                     /note="Hammerhead ribozyme"
     misc_feature    2179..2203
                     /label=Mature repeat after processing
     misc_recomb     2204..2235
                     /label=Guide RNA cloning site
                     /note="Guide RNA cloning site"
     stem_loop       2236..2303
                     /label=HDV ribozyme
                     /note="HDV ribozyme"
     misc_feature    2323..2422
                     /label=attL2
     misc_feature    2452..2486
                     /label=extra seq
                     /note="extra seq"
     primer_bind     complement(2494..2514)
                     /label=M13-rev
     misc_feature    2592..2725
                     /label=Spn promoter
     misc_feature    2726..3736
                     /label=SpnR
     misc_feature    3803..4476
                     /label=pUC origin
ORIGIN
        1 tttcccagtc acgacgttgt aaaacgacgg ccagtcttaa gctcgggccc aaataatgat
       61 tttattttga ctgatagtga cctgttcgtt gcaacaaatt gatgagcaat gcttttttat
      121 aatgccaact ttgtatacaa aagttgcccc atggcgttcc ctctagagag ataatgagca
      181 ttgcatgtct aagttataaa aaattaccac atattttttt tgtcacactt gtttgaagtg
      241 cagtttatct atctttatac atatatttaa actttactct acgaataata taatctatag
      301 tactacaata atatcagtgt tttagagaat catataaatg aacagttaga catggtctaa
      361 aggacaattg agtattttga caacaggact ctacagtttt atctttttag tgtgcatgtg
      421 ttctcctttt tttttgcaaa tagcttcacc tatataatac ttcatccatt ttattagtac
      481 atccatttag ggtttagggt taatggtttt tatagactaa tttttttagt acatctattt
      541 tattctattt tagcctctaa attaagaaaa ctaaaactct attttagttt ttttatttaa
      601 taatttagat ataaaataga ataaaataaa gtgactaaaa attaaacaaa taccctttaa
      661 gaaattaaaa aaactaagga aacatttttc ttgtttcgag tagataatgc cagcctgtta
      721 aacgccgtcg acgagtctaa cggacaccaa ccagcgaacc agcagcgtcg cgtcgggcca
      781 agcgaagcag acggcacggc atctctgtcg ctgcctctgg acccctctcg agagttccgc
      841 tccaccgttg gacttgctcc gctgtcggca tccagaaatt gcgtggcgga gcggcagacg
      901 tgagccggca cggcaggcgg cctcctcctc ctctcacggc accggcagct acgggggatt
      961 cctttcccac cgctccttcg ctttcccttc ctcgcccgcc gtaataaata gacaccccct
     1021 ccacaccctc tttccccaac ctcgtgttgt tcggagcgca cacacacaca accagatctc
     1081 ccccaaatcc acccgtcggc acctccgctt caaggtacgc cgctcgtcct cccccccccc
     1141 cccctctcta ccttctctag atcggcgttc cggtccatgg ttagggcccg gtagttctac
     1201 ttctgttcat gtttgtgtta gatccgtgtt tgtgttagat ccgtgctgct agcgttcgta
     1261 cacggatgcg acctgtacgt cagacacgtt ctgattgcta acttgccagt gtttctcttt
     1321 ggggaatcct gggatggctc tagccgttcc gcagacggga tcgatttcat gatttttttt
     1381 gtttcgttgc atagggtttg gtttgccctt ttcctttatt tcaatatatg ccgtgcactt
     1441 gtttgtcggg tcatcttttc atgctttttt ttgtcttggt tgtgatgatg tggtctggtt
     1501 gggcggtcgt tctagatcgg agtagaatta attctgtttc aaactacctg gtggatttat
     1561 taattttgga tctgtatgtg tgtgccatac atattcatag ttacgaattg aagatgatgg
     1621 atggaaatat cgatctagga taggtataca tgttgatgcg ggttttactg atgcatatac
     1681 agagatgctt tttgttcgct tggttgtgat gatgtggtgt ggttgggcgg tcgttcattc
     1741 gttctagatc ggagtagaat actgtttcaa actacctggt gtatttatta attttggaac
     1801 tgtatgtgtg tgtcatacat cttcatagtt acgagtttaa gatggatgga aatatcgatc
     1861 taggataggt atacatgttg atgtgggttt tactgatgca tatacatgat ggcatatgca
     1921 gcatctattc atatgctcta accttgagta cctatctatt ataataaaca agtatgtttt
     1981 ataattattt tgatcttgat atacttggat gatggcatat gcagcagcta tatgtggatt
     2041 tttttagccc tgccttcata cgctatttat ttgcttggta ctgtttcttt tgtcgatgct
     2101 caccctgttg tttggtgtta cttctgcagg gatcctcgtt gctgatgagt ccgtgaggac
     2161 gaaacgagta agctcgtcca acgattgccc ctcacgaggg gacgagacgg agctcagtct
     2221 gaccgcggcg tctctggccg gcatggtccc agcctcctcg ctggcgccgg ctgggcaaca
     2281 tgcttcggca tggcgaatgg gacggtaccc ggccggaatt cgacccagct ttcttgtaca
     2341 aagttggcat tataaaaaat aattgctcat caatttgttg caacgaacag gtcactatca
     2401 gtcaaaataa aatcattatt tgccatccag ctgatatccc ctatagtgag tcgtattaca
     2461 tggtcatagc atcccctata gtgagtcgta ttacatggtc atagctgttt cctggcagct
     2521 ctggcccgtg tctcaaaatc tctgatgtta cattgcacaa gataaaaata tatcatcatg
     2581 cctcctctgg accagccagg acagaaatgc ctcgacttcg ctgctgccca aggttgccgg
     2641 gtgacgcaca ccgtggaaac ggatgaaggc acgaacccag tggacataag cctgttcggt
     2701 tcgtaagctg taatgcaagt agcgtatgcg ctcacgcaac tggtccagaa ccttgaccga
     2761 acgcagcggt ggtaacggcg cagtggcggt tttcatggct tgttatgact gtttttttgg
     2821 ggtacagtct atgcctcggg catccaagca gcaagcgcgt tacgccgtgg gtcgatgttt
     2881 gatgttatgg agcagcaacg atgttacgca gcagggcagt cgccctaaaa caaagttaaa
     2941 catcatgagg gaagcggtga tcgccgaagt atcgactcaa ctatcagagg tagttggcgt
     3001 catcgagcgc catctcgaac cgacgttgct ggccgtacat ttgtacggct ccgcagtgga
     3061 tggcggcctg aagccacaca gtgatattga tttgctggtt acggtgaccg taaggcttga
     3121 tgaaacaacg cggcgagctt tgatcaacga ccttttggaa acttcggctt cccctggaga
     3181 gagcgagatt ctccgcgctg tagaagtcac cattgttgtg cacgacgaca tcattccgtg
     3241 gcgttatcca gctaagcgcg aactgcaatt tggagaatgg cagcgcaatg acattcttgc
     3301 aggtatcttc gagccagcca cgatcgacat tgatctggct atcttgctga caaaagcaag
     3361 agaacatagc gttgccttgg taggtccagc ggcggaggaa ctctttgatc cggttcctga
     3421 acaggatcta tttgaggcgc taaatgaaac cttaacgcta tggaactcgc cgcccgactg
     3481 ggctggcgat gagcgaaatg tagtgcttac gttgtcccgc atttggtaca gcgcagtaac
     3541 cggcaaaatc gcgccgaagg atgtcgctgc cgactgggca atggagcgcc tgccggccca
     3601 gtatcagccc gtcatacttg aagctagaca ggcttatctt ggacaagaag aagatcgctt
     3661 ggcctcgcgc gcagatcagt tggaagaatt tgtccactac gtgaaaggcg agatcaccaa
     3721 ggtagtcggc aaataaccct cgagccaccc atgaccaaaa tcccttaacg tgagttacgc
     3781 gtcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
     3841 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
     3901 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
     3961 gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt
     4021 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
     4081 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
     4141 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
     4201 gagataccta cagcgtgagc attgagaaag cgccacgctt cccgaaggga gaaaggcgga
     4261 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
     4321 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
     4381 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
     4441 acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga
     4501 ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac
     4561 gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc
     4621 tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa
     4681 agcgggcagt gagcgcaacg caattaatac gcgtaccgct cgccaggaag agtttgtaga
     4741 aacgcaaaaa ggccatccgt caggatggcc ttctgcttag tttgatgcct ggcagtttat
     4801 ggcgggcgtc ctgcccgcca ccctccgggc cgttgcttca caacgttcaa atccgctccc
     4861 ggcggatttg tcctactcag gagagcgttc accgacaaac aacagataaa acgaaaggcc
     4921 cagtcttccg actgagcctt tcgttttatt tgatgcctgg cagttcccta ctctcgcgtt
     4981 aacgcttgca tggatgt
//
