LOCUS       Exported               12598 bp ds-DNA    circular SYN 14-7月-2021
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    pDUAL-41nmt1p-Pil1-mCherry-Vps38
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12598)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported 2021年7月14日 from SnapGene 2.3.2
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..12598
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        36..1200
                     /note="nmt41 promoter"
     CDS             1213..2265
                     /codon_start=1
                     /note="Pil1"
                     /translation="MMNRAYSLRNSRAPTASQLLNPPPPSSSTRSGRLFAPLSHTMRIN
                     HAATFTPDLAKRLAVLVKMEKNVMRSMEVTIRGRRDCARQLSYWGEDCDDDISDVTDKL
                     GVLFYEMAELENYLIDRYDQYRMTLKSIRNIESSVQPSREKKQKLLDQIYALKHKDPES
                     PRLVTMEQELVREEAACLVAEAQLSNTTREKFKQAMTFNLDALHEHAEKLNLIATYGRH
                     LLNLIDDTPVTPGEARPAYDGYETSRQIVMDAEHALSSWVPSQPAVNFVKPQIEDDAQS
                     DARSWNEYEAQGEPVPVEQVTHLQDDVQSDTSEIIENEPGMHPHVHAERMSRIASESSD
                     AVPQQTAVQVA"
     CDS             2272..2979
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent 
                     protein"
                     /note="mCherry"
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     misc_feature    2989..4707
                     /note="vps38(with intron)"
     CDS             2989..3225
                     /codon_start=1
                     /note="vps38 extron1"
                     /translation="MSLELPGNYRLSRLKSIQIRNVQEIQNHSKFAINTTENLWKRDVQ
                     LKRAISEGTIRIFISLHVQSKKLPVYITETSGNA"
     intron          3226..3455
                     /note="vps38 intron1"
     CDS             3456..3626
                     /codon_start=1
                     /note="vps38 extron2"
                     /translation="NHIFYVDEKVTEKLQKYRHEEYFIVRTWCSSSSHAFKLHKEWKIL
                     RYDSNFRYIGND"
     intron          3627..3690
                     /note="vps38 intron2"
     CDS             3691..4707
                     /codon_start=1
                     /note="vps38 extron3"
                     /translation="PVFAVCHIRNGLLCEFNDGVYIYTTSQSSDIMRQTSFPKSASTYS
                     IDRRKDGYTIQKITRILKLAECIDEMHIAKHEIRAHFQEEEFQQIRLMHKRMLLRDEKI
                     DELAKLEHLWQKQINSITQMRTKFDKTKSWLSSKRNTLNKSKESLQKDEAEYVELANSL
                     KTKVETNIEIRILMAHAIRMHVSHLSKIYPIQPSPGNHDEFTIRNLRLSFEPDKINNVE
                     MAASIGFLAHLLQTLSKYLEKELAYPILCASSRSSILDTLTPDIPTRIFPLYPATRPIE
                     LFEHAIYLLNQDVNDFLETFGLPIDQSMDILRNFKKLLQFILSGQHLSFVQVTSTAP"
     terminator      4734..4921
                     /gene="S. cerevisiae ADH1"
                     /note="ADH1 terminator"
                     /note="transcription terminator for alcohol dehydrogenase 
                     1"
     primer_bind     complement(9494..9510)
                     /note="M13 fwd"
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      9723..10178
                     /direction=RIGHT
                     /note="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        10460..10564
                     /gene="bla"
                     /note="AmpR promoter"
     CDS             10565..11425
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      11596..12184
                     /direction=RIGHT
                     /note="ori"
                     /note="high-copy-number colE1/pMB1/pBR322/pUC origin of 
                     replication"
     promoter        12508..12538
                     /note="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    12546..12562
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="lac operator"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     12570..12586
                     /note="M13 rev"
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 aagcttgcat gcctgcaggt cgatcgactc tagaggatca gaaaattatc gccataaaag
       61 acagaataag tcatcagcgg ttgtttcatt tcctatattt tttttttatt tttttatttt
      121 ttaataaggg aaaatttaac gtctaaggat acagaagatt gttagcacat taaagtaata
      181 aaggcttaag tagtaagtgc cttagcatgt tattgtattt caaaggacat aatctaaaat
      241 aataacaata tcatttctca caagttattc aattttcttt tttttttcta ataatatcaa
      301 gaatgtatta tttgtttgac ataagtcaac taatttattt aatatgctgg attaatcttg
      361 cagacatgta aattaacaag ttttagtcaa ataacgttga agtttcaatg aactcaaata
      421 atttctcttt ttttttatat aactctaatc tgatttatat tttccgcagg gatcaactga
      481 agttatgaca tttggattgg atcacttata accttggtcg ccaaataata caaaaatcag
      541 cgttataaaa caaagaaggt ttttgttaag aaattaatcc tctttcttga taagaaagtt
      601 gaaccgaaat tgcagatact gatatatgaa aataataccc acaattttgg gaatagcgca
      661 agcctcaatt taaacaatag gtgaggacac atgataatga cctcaatgat tgttagaaga
      721 aaagagcctc attacaaaat cgaaaaatga atggttgggt acaagtttcc aaaacatggt
      781 aaagtggact ttgcgtatga gacgtaaata gaaaaaaaca cttgttatat gttttctaga
      841 attattgttg tctctttatg gttggatgat gcaaaatagt aatttcggtt agttgctgta
      901 aaacaccacg agacaaatag atatggatat ttattaaatc aggaaaaacg taactctcgg
      961 ctactggatg gttcagtcac ccaacgatta ctggggagag aaaacagggc aaaagcaaag
     1021 cttaaaggaa tccgattgtc attcggcaat gtgcagcgaa actaaaaacc ggataatgga
     1081 cctgttaatc gaaacattga agataaagga agaggaatcc tggcatatca tcaattgaat
     1141 aagttgaatt aattatttca atctcattct cactttctga cttatagtcg ctttgttaaa
     1201 gctagcggat ccatgatgaa ccgtgcgtac tctttaagaa acagccgtgc ccctacggcc
     1261 tcacaattgt taaacccccc tcctccctcc tcttccactc gtagtggtcg tttgtttgcc
     1321 cccttgagtc ataccatgcg tatcaaccac gctgccacct tcacccctga cttggctaag
     1381 cgtttggctg tcttggtcaa gatggaaaag aacgtgatgc gcagtatgga ggtcaccatc
     1441 cgtggccgtc gtgattgtgc tcgccaactt tcctactggg gtgaggactg cgatgacgat
     1501 atcagcgacg tcaccgacaa attgggtgtc ttgttttacg agatggccga gttggagaac
     1561 tacttgatcg atcgttatga ccaataccgt atgacgttga agtccatccg taacatcgag
     1621 tcctctgtcc aaccctctcg tgagaagaag caaaagctct tggatcaaat ctatgctctt
     1681 aagcataagg atcctgaatc ccctcgcttg gtcaccatgg agcaagagct tgttcgtgag
     1741 gaggctgcct gtttggttgc cgaggctcaa ctttccaaca ccacccgtga aaagttcaag
     1801 caagccatga ccttcaactt ggatgccctc cacgagcatg ccgagaagct caacctcatt
     1861 gccacctatg gcagacattt gttgaacttg attgacgaca cccccgtcac tcccggtgag
     1921 gctcgtcctg cctacgatgg ttatgagact tcccgccaaa tcgtcatgga tgctgagcac
     1981 gccttgagct cttgggttcc cagccaaccc gccgtcaact ttgtcaagcc tcaaatcgag
     2041 gatgatgccc aaagtgacgc tcgttcctgg aacgagtatg aggctcaagg tgagcccgtc
     2101 cccgttgagc aagtcaccca cttacaagac gacgttcaat ccgacactag cgagatcatt
     2161 gagaacgagc ctggcatgca ccctcatgtc catgctgagc gtatgtctcg cattgcttct
     2221 gagtctagcg atgccgttcc tcagcaaacc gccgtccagg ttgctggatc catggtgagc
     2281 aagggcgagg aggataacat ggccatcatc aaggagttca tgcgcttcaa ggtgcacatg
     2341 gagggctccg tgaacggcca cgagttcgag atcgagggcg agggcgaggg ccgcccctac
     2401 gagggcaccc agaccgccaa gctgaaggtg accaagggtg gccccctgcc cttcgcctgg
     2461 gacatcctgt cccctcagtt catgtacggc tccaaggcct acgtgaagca ccccgccgac
     2521 atccccgact acttgaagct gtccttcccc gagggcttca agtgggagcg cgtgatgaac
     2581 ttcgaggacg gcggcgtggt gaccgtgacc caggactcct ccctgcagga cggcgagttc
     2641 atctacaagg tgaagctgcg cggcaccaac ttcccctccg acggccccgt aatgcagaag
     2701 aagaccatgg gctgggaggc ctcctccgag cggatgtacc ccgaggacgg cgccctgaag
     2761 ggcgagatca agcagaggct gaagctgaag gacggcggcc actacgacgc tgaggtcaag
     2821 accacctaca aggccaagaa gcccgtgcag ctgcccggcg cctacaacgt caacatcaag
     2881 ttggacatca cctcccacaa cgaggactac accatcgtgg aacagtacga acgcgccgag
     2941 ggccgccact ccaccggcgg catggacgag ctgtacaagg gtagatctat gtcgcttgaa
     3001 ttaccaggaa actaccgact ttccaggctt aaatcaatac aaataagaaa tgttcaagaa
     3061 attcaaaatc atagcaaatt tgcgatcaat acgacggaaa atttgtggaa acgagatgtg
     3121 cagttaaaac gtgcaatttc cgaaggaact attcgtattt ttatatcatt gcatgtgcag
     3181 agtaagaaat tgcctgttta tattactgaa acgagcggaa atgctgtatg tgaacatgct
     3241 ttgataaaca aaagtatctg aatggtatca ttatatctag gttatttgct tgattgaaaa
     3301 ggttaatcag tttgtttata caagtgattt gccaaaattt cgttgctcaa atccttgaaa
     3361 caaagccctc aattgatagt ttataccttg tatctctttt acccatatac aaattttgta
     3421 tgtatttatg ttctttgtgt ctaacctctc attagaatca catcttttac gtcgatgaaa
     3481 aagtaaccga aaagcttcaa aaataccgtc acgaagagta ctttatcgtg aggacttggt
     3541 gctcatcaag tagtcatgcc ttcaagctac ataaagaatg gaaaattttg agatatgaca
     3601 gtaattttcg ttatattggt aatgatgtac ggttgaatat ttttggttta ccttatgttt
     3661 cccttgtgtt gactaacata ttatttgtag cctgtttttg cagtttgtca tattcgaaac
     3721 ggtcttttat gtgaatttaa tgatggcgtt tacatctaca caacatccca gtcttcggat
     3781 attatgagac agacctcctt tccaaaaagt gcctccacat attctataga caggcggaag
     3841 gatggttaca ctattcagaa aatcactcga attttgaaac tggctgaatg catagatgaa
     3901 atgcacattg ctaaacatga aattcgagct cattttcagg aggaggagtt tcagcagata
     3961 cgtttgatgc acaaaaggat gttacttcgg gatgaaaaaa tcgatgaact ggctaaatta
     4021 gaacatcttt ggcaaaaaca aattaatagc atcactcaga tgaggacgaa atttgacaag
     4081 acgaaaagct ggttatcctc aaaaaggaac actttgaaca aatccaaaga atcccttcaa
     4141 aaagatgaag ctgagtatgt ggaacttgcc aattctttaa aaacaaaagt agagaccaat
     4201 atagagattc gaatccttat ggcacatgct attcgtatgc acgtttccca tctttccaaa
     4261 atttacccaa tccaaccatc gcctggcaat catgacgagt ttacaattcg caatctccgc
     4321 ctatcttttg agccagataa aattaataat gtggaaatgg ctgcttccat aggatttttg
     4381 gcgcatttac ttcaaactct ttcaaagtat ctcgaaaaag aactcgctta tccaattctt
     4441 tgcgcatctt cgagatcctc catcctggat acattgacac ccgacatccc cacgcgtatc
     4501 tttccccttt atccagcaac tcgaccaatt gagctttttg aacatgccat ttatcttctc
     4561 aaccaggatg taaatgattt tttagaaact tttggattac ctattgacca atccatggac
     4621 atactcagga actttaaaaa actgctccaa tttattttat caggacaaca tctatctttt
     4681 gtacaagtaa cgtctaccgc tccttaaaga tctggcgcgc cacttctaaa taagcgaatt
     4741 tcttatgatt tatgattttt attattaaat aagttataaa aaaaataagt gtatacaaat
     4801 tttaaagtga ctcttaggtt ttaaaacgaa aattcttatt cttgagtaac tctttcctgt
     4861 aggtcaggtt gctttctcag gtatagtatg aggtcgctct tattgaccac acctctaccg
     4921 gcagatccgc tagggataac agggtaatat actagtaaga tgattctttt atcgtcgata
     4981 acgatgagga tgtagacgat tatgatgaga gtgattagct tctaaaattt ggtgttttca
     5041 aacgctattt acagtacaat ctgaaaaaat tggatgaaca ttgtaaatgg taggatacga
     5101 gttccaatta atgtattttt taagtattac acttagctta ttaattttat atatacattc
     5161 tacctctgtg ggtttaaaga caactcgtac acatactcaa attatttatg aacattataa
     5221 tgcatattcg tactgtctat taaacatttt tttgacatta aaaatttcgt ttactaacgt
     5281 agaaagcgct gataattgag gctactattt taatatctag aaacaaagat tcattaatta
     5341 attaagtaag ctccaggata cttgtatatt tcgttaaaaa agcgcaattt caacaattcc
     5401 tatgaacatc catgatttca tcacaaaatc aaacatatca ggatttaacg tgaattattt
     5461 gctttcttgt gattttcatc tgatttctgg tcatttacgt tactgtaaac aagcttttta
     5521 aaaaaaggat tttttacgca atattatcga tatcccaatc tgtagtataa aaagtgcaac
     5581 agtgttgagt ttcccgaaac caggcatttt tgcggtaggg tctgaaactt tcacgaaacc
     5641 atttcacaac aaaacttctt caacaacatg tgtgcaaaga aaatcgtcgt cttaccagga
     5701 gaccatattg gccctgaaat tgttgcttct gccttggagg ttttgaaagt cgttgagaag
     5761 aagcgacctg agttaaaact cgagtttgaa gaacacaaga ttggaggtgc ctctattgat
     5821 gcctatggaa cccctttgac tgacgagact gtgaaggctt gtttggaagc tgacggtgtt
     5881 cttttgggtg ccgttggtgg tcctgaatgg accaacccca attgtcgtcc tgagcaaggt
     5941 ttattgaagc ttcgtaagag tatgggtgtt tgggccaacc ttcgaccttg caactttgcc
     6001 agcaagtctt tagtcaagta cagccctttg aagcctgaaa tcgttgaagg tgtcgatttt
     6061 tgtgttgtac gagaacttac tggaggttgt tactttggtg agcgcactga ggacaacgga
     6121 tcgggttatg ctatggacac ttggccttac agtttggaag aagtttctcg tattgctcgt
     6181 ttggcggccg cgggcccaac accaatgttt ataaccaagt tttatcttgt ttgtctacat
     6241 ggtattttac attcatctac atacatcttt cattggcttt gtacatagtt atcattacaa
     6301 gtctaaaaaa attcactctt ttcttattca atgtcaatcc aagagaaaag attgtggtaa
     6361 tgttgtagga gcatgtttaa taaattacta tagcaaatta ctttttattc ccaaggtgtt
     6421 tatctataat agttaatatt ttagtcgcta cataaaattt taccaaagag tacttgtata
     6481 ctaattctaa atgccttctg acataaaacg cctaggaaaa caaacgcaaa caaggcatcg
     6541 actttttcaa taaccaacca aaaaaatttt acattagtct ttttttaatg ctgagaaagt
     6601 ctttgctgat atgccttcca accagcttct ctatatctct tggcttcgac aacaggatta
     6661 cgaccagctc catagactcc acgaccaaca atgatgatgt cgctaccgca gtttacaatc
     6721 acttcttcag gagtacgata ttgctgtccc agcccgtctc ctttaacatc caagccgata
     6781 ccaggggaca tagttatgta gtcgctttga aggttaggaa atcgacgacc agctataaag
     6841 ccaaagcaaa aatcggtatg cttctcaaac cattctaagg ttttctctgt gtaggaacca
     6901 gtagccaaag agcctttgga agacatttca gccaaaagca agagaccacg tcccaaaggt
     6961 aaaccaactt ctttgaggcc ttgtataata ccctcgcctg gcactgtatg gcaatttgtg
     7021 atatgagccc aagaagcaat tttgtacaca ccagatgcat attgtagctt gacggtattt
     7081 ccaatgtctg cgaatttgcg atcctcaaag ataagaaaac gatgcttttt acctaaggcc
     7141 accagttttt ctaccatatc ctggtcgaaa tcctcgacaa cgtcaatatg tgtcttgata
     7201 acacagacat agggtccaat tttatctacc aattctaaga tttcggattt cttcgtcaaa
     7261 tcgaccgcga ctgacaagtt gctttgcttt tcttccatca aagccaacaa ttccttggca
     7321 atgggatttt tcatcccctc agctctagct gaatagcttt gaaatactct agcatccata
     7381 actttgcttt taaaccttta atttcgatcc aagcaaaaaa gaggttcttg gtaggacaat
     7441 acggtaagaa aacacgacat gtgcagagat gccgacgaag catagttaaa ctgggatggt
     7501 aaaatcaatt aagaatttat aaagacaaaa ttgtataagt ctctaaaaca tcttaattat
     7561 acctcacaga actatctaaa atatattcac aaagtgcaaa cattatcatg aaaaagaacc
     7621 attttaattt aaagcaaggg cattaaggct tatttacaga atttcttact tttgtaaaga
     7681 ttataaggct gattatcttt ttcaccatgc caaaaattac acaagataga atggatgttt
     7741 gaaattaaac gtgagtatac aaacaaatac actaggtaaa tcgaaacatt tttttctcca
     7801 ttaagtaaca aattcctatt tagagaaaga atgctgagta gattaaataa tctatacaaa
     7861 cttttttaac acaaatgcat acatatagcc agtgggattt gtagctaagc ttccgaattc
     7921 gagtctaact ccttaaccac tcggacatag tgacttatct gacactcatt gtaaattaat
     7981 atatatatag gcattttgtt tagttaaagg tacttaagta attagtataa acgaaccaat
     8041 tttataatca ggaagttaag tgaatggtag cacatgtcgt aaaaattgtg aatttttatt
     8101 gaataatatt ttaaatacaa gcctttctag actaggtata ctcataaaca tatatgagca
     8161 aaaggataga ggagattaca ttgcatcttc tacaaattta tttattgccc tttactgaaa
     8221 aattaaataa tgagtactaa atgataaaaa gcgctcagta caagaaagat tgcaaaaata
     8281 ttgcattctt catgaattaa agttgcatat aaggcatatt gaaagtaata gtactaaaac
     8341 agcagttagc gaaaattaat agaattatat tcgcaagaca attgtgacat taaattaaaa
     8401 aattgtagaa tttttactat cctctttaac gccatgagcc tttataaaaa ggttaaatta
     8461 gttttaacat tctttttttg agtaagagtt tacaatttat caaaacctgt gttattatat
     8521 tcattagttt caatttatta gcatctagag aaaaatcaat tggcagttac attgttggaa
     8581 tttatgaaga aaaagaatct acaacggaga ataagttgct gatcgctttc cctaaaattg
     8641 tatattttgc tgagcttatt ttgacatttc gttgaagttt tctctaattc gcattcattt
     8701 taagtaaaac aatgagaaat aaaattacaa aaaatacaaa ttaaaataca atttttagct
     8761 ataattatag acgatgccct tgtatcccat tctgtctcgc ttgcccctac tttttatctt
     8821 ttatatacca taatgaacgc tgccgctact aaccataccc cgattttaca tttcggactc
     8881 ccaaggacgt acaaaataga aaactataga aaaaaataat cagaaaatag catgtcatct
     8941 ctttgtaaaa cgcgtttgca agaagaaagg aaacaatgga gaagagatca tccatttgta
     9001 tgtaaatttt agtaaacttg aagaaatcac taacaacttc tcttacttag ggattctatg
     9061 caaaaccttg taaatcatct gatggaggac tcgatttaat gaattggaag gttgggcccg
     9121 cggccgctta gctgaaactt ccaaccctcc tgctcccgtc acattactcg acaaagctaa
     9181 tgttttggca acttctcgtc tttggcgcaa gactgtagct aagattttta aggaggaata
     9241 tcctcacctt accttaaaaa accagctcat tgactccgca gccatgcttt tggtcaagag
     9301 ccctcgtaca cttaacggtg ttgttttgac tgacaacttg tttggtgaca ttatttcaga
     9361 tgaggcttct gtcattcctg gtagcttggg ccttttgcct tctgcctccc tttccggtgt
     9421 ggtaggaaaa tcagaagaaa aggttcattg tttggttgag cccattcacg gtagcgctcc
     9481 cgagctcgaa ttcactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta
     9541 cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg
     9601 cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg cgcctgatgc
     9661 ggtattttct ccttacgcat ctgtgcggta tttcacaccg catacgtcaa agcaaccata
     9721 gtacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac
     9781 cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc
     9841 cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt
     9901 tagtgcttta cggcacctcg accccaaaaa acttgatttg ggtgatggtt cacgtagtgg
     9961 gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag
    10021 tggactcttg ttccaaactg gaacaacact caaccctatc tcgggctatt cttttgattt
    10081 ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt
    10141 taacgcgaat tttaacaaaa tattaacgtt tacaatttta tggtgcactc tcagtacaat
    10201 ctgctctgat gccgcatagt taagccagcc ccgacacccg ccaacacccg ctgacgcgcc
    10261 ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag
    10321 ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgagacgaa agggcctcgt
    10381 gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg
    10441 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
    10501 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
    10561 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct
    10621 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg
    10681 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg
    10741 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt
    10801 atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga
    10861 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga
    10921 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac
    10981 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg
    11041 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac
    11101 gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct
    11161 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct
    11221 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg
    11281 gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat
    11341 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg
    11401 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat
    11461 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
    11521 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
    11581 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
    11641 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
    11701 gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta
    11761 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
    11821 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
    11881 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
    11941 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
    12001 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
    12061 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
    12121 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
    12181 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
    12241 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
    12301 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
    12361 ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag
    12421 ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag
    12481 ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg
    12541 tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg attacgcc
//
