LOCUS       Exported                3643 bp DNA     circular SYN 12-JUL-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3643)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 13, 2021 from SnapGene Viewer 5.3.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3643
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             complement(239..307)
                     /label=LacZ alpha
     misc_feature    335..354
                     /label=PCR donor Forward Primer
     misc_feature    359..375
                     /label=M13 -40
     primer_bind     378..395
                     /label=M13-fwd
     misc_feature    396..418
                     /label=gBait gRNA
     misc_feature    422..429
                     /label=NotI
     misc_feature    430..435
                     /label=AgeI
     misc_feature    436..441
                     /label=BamHI
     misc_feature    442..447
                     /label=EcoRI
     misc_feature    448..453
                     /label=NheI
     misc_feature    454..459
                     /label=SacII
     misc_feature    460..465
                     /label=XbaI
     misc_feature    466..471
                     /label=XhoI
     misc_feature    472..1167
                     /label=mScarlet
     misc_feature    1168..1173
                     /label=SpeI
     misc_feature    1174..1179
                     /label=ClaI
     misc_feature    1180..1185
                     /label=HindIII
     misc_feature    1186..1191
                     /label=SacI
     misc_feature    1192..1197
                     /label=SalI
     polyA_signal    1201..1435
                     /label=ubb polyA sequence
     misc_binding    complement(1444..1465)
                     /label=LacO
     misc_feature    1466..1485
                     /label=PCR donor Reverse Primer
     rep_origin      complement(1806..2488)
                     /direction=LEFT
                     /label=ColE1 origin
     CDS             complement(2586..3245)
                     /label=AmpR
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtggcga gggcgatgcc acctacggga
      421 tgcggccgca ccggtggatc cgaattcgct agcccgcggt ctagactcga ggtgagcaag
      481 ggcgaggcag tgatcaagga gttcatgcgg ttcaaggtgc acatggaggg ctccatgaac
      541 ggccacgagt tcgagatcga gggcgagggc gagggccgcc cctacgaggg cacccagacc
      601 gccaagctga aggtgaccaa gggtggcccc ctgcccttct cctgggacat cctgtcccct
      661 cagttcatgt acggctccag ggccttcacc aagcaccccg ccgacatccc cgactactat
      721 aagcagtcct tccccgaggg cttcaagtgg gagcgcgtga tgaacttcga ggacggcggc
      781 gccgtgaccg tgacccagga cacctccctg gaggacggca ccctgatcta caaggtgaag
      841 ctccgcggca ccaacttccc tcctgacggc cccgtaatgc agaagaagac aatgggctgg
      901 gaagcgtcca ccgagcggtt gtaccccgag gacggcgtgc tgaagggcga cattaagatg
      961 gccctgcgcc tgaaggacgg cggccgctac ctggcggact tcaagaccac ctacaaggcc
     1021 aagaagcccg tgcagatgcc cggcgcctac aacgtcgacc gcaagttgga catcacctcc
     1081 cacaacgagg actacaccgt ggtggaacag tacgaacgct ccgagggccg ccactccacc
     1141 ggcggcatgg acgagctgta caagtaaact agtatcgata agcttgagct cgtcgacatc
     1201 attctcagta tcccctgctt atgcactcac cactttttcc cattatttta ttcatgttgt
     1261 gttgctaaat tcaactacat tcgtattacc atgttgtttc atttgttttt tttttatctc
     1321 agctgtcaaa ataaaagcat tgaaatacaa cattgttcta tttttcattg tggttttgca
     1381 tgcccggtaa ataaaaaaac aaggtaataa aaataagatg cgaaatgaga agtatatctg
     1441 tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa
     1501 gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct
     1561 ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga
     1621 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
     1681 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
     1741 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
     1801 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
     1861 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     1921 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     1981 tccgcctttc tcccttcggg aagcgtggcg ctttctcaat gctcacgctg taggtatctc
     2041 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     2101 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     2161 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     2221 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc
     2281 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     2341 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     2401 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
     2461 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
     2521 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
     2581 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
     2641 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
     2701 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata
     2761 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
     2821 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
     2881 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
     2941 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
     3001 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
     3061 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
     3121 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
     3181 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg
     3241 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
     3301 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
     3361 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
     3421 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
     3481 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
     3541 gttccgcgca catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg
     3601 acattaacct ataaaaatag gcgtatcacg aggccctttc gtc
//
