LOCUS       Exported                3664 bp DNA     circular SYN 12-JUL-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3664)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 13, 2021 from SnapGene Viewer 5.3.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3664
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             complement(239..307)
                     /label=LacZ alpha
     misc_feature    335..354
                     /label=PCR donor Forward Primer
     misc_feature    359..375
                     /label=M13 -40
     primer_bind     378..395
                     /label=M13-fwd
     misc_feature    396..418
                     /label=gBait gRNA
     misc_feature    422..429
                     /label=NotI
     misc_feature    430..435
                     /label=AgeI
     misc_feature    436..441
                     /label=BamHI
     misc_feature    442..447
                     /label=EcoRI
     misc_feature    448..453
                     /label=NheI
     misc_feature    454..459
                     /label=SacII
     misc_feature    460..465
                     /label=XbaI
     misc_feature    466..471
                     /label=XhoI
     misc_feature    472..1188
                     /label=eGFP no start / stop
     misc_feature    1189..1194
                     /label=SpeI
     misc_feature    1195..1200
                     /label=ClaI
     misc_feature    1201..1206
                     /label=HindIII
     misc_feature    1207..1212
                     /label=SacI
     misc_feature    1213..1218
                     /label=SalI
     polyA_signal    1222..1456
                     /label=ubb polyA sequence
     misc_binding    complement(1465..1486)
                     /label=LacO
     misc_feature    1487..1506
                     /label=PCR donor Reverse Primer
     rep_origin      complement(1827..2509)
                     /direction=LEFT
                     /label=ColE1 origin
     CDS             complement(2607..3266)
                     /label=AmpR
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtggcga gggcgatgcc acctacggga
      421 tgcggccgca ccggtggatc cgaattcgct agcccgcggt ctagactcga ggtgagcaag
      481 ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac
      541 ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc
      601 ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc
      661 ctgacctacg gcgtgcagtg cttcagccgc taccccgacc acatgaagca gcacgacttc
      721 ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac
      781 ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc
      841 gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac
      901 aactacaaca gccacaacgt ctatatcatg gccgacaagc agaagaacgg catcaaggtg
      961 aacttcaaga tccgccacaa catcgaggac ggcagcgtgc agctcgccga ccactaccag
     1021 cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagcacc
     1081 cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc
     1141 gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagtaaac tagtatcgat
     1201 aagcttgagc tcgtcgacat cattctcagt atcccctgct tatgcactca ccactttttc
     1261 ccattatttt attcatgttg tgttgctaaa ttcaactaca ttcgtattac catgttgttt
     1321 catttgtttt ttttttatct cagctgtcaa aataaaagca ttgaaataca acattgttct
     1381 atttttcatt gtggttttgc atgcccggta aataaaaaaa caaggtaata aaaataagat
     1441 gcgaaatgag aagtatatct gtgaaattgt tatccgctca caattccaca caacatacga
     1501 gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt
     1561 gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga
     1621 atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc
     1681 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
     1741 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
     1801 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
     1861 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
     1921 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
     1981 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcaa
     2041 tgctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
     2101 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     2161 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
     2221 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
     2281 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
     2341 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
     2401 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
     2461 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
     2521 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
     2581 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
     2641 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
     2701 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
     2761 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
     2821 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
     2881 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
     2941 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
     3001 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
     3061 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
     3121 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
     3181 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
     3241 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
     3301 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
     3361 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
     3421 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
     3481 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
     3541 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc
     3601 taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt
     3661 cgtc
//
