LOCUS       Exported                4066 bp DNA     circular SYN 12-JUL-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4066)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 13, 2021 from SnapGene Viewer 5.3.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4066
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             complement(239..307)
                     /label=LacZ alpha
     misc_feature    335..354
                     /label=PCR donor Forward Primer
     misc_feature    359..375
                     /label=M13 -40
     primer_bind     378..395
                     /label=M13-fwd
     misc_feature    396..418
                     /label=gBait gRNA
     misc_feature    422..429
                     /label=NotI
     misc_feature    430..435
                     /label=AgeI
     misc_feature    436..441
                     /label=BamHI
     misc_feature    442..447
                     /label=EcoRI
     misc_feature    448..453
                     /label=NheI
     misc_feature    454..459
                     /label=SacII
     misc_feature    460..465
                     /label=XbaI
     misc_feature    466..471
                     /label=XhoI
     misc_feature    472..537
                     /label=p2A peptide from Tol2kit
     misc_feature    538..1590
                     /label=QF2
     misc_feature    1591..1596
                     /label=SpeI
     misc_feature    1597..1602
                     /label=ClaI
     misc_feature    1603..1608
                     /label=HindIII
     misc_feature    1609..1614
                     /label=SacI
     misc_feature    1615..1620
                     /label=SalI
     polyA_signal    1624..1858
                     /label=ubb polyA sequence
     misc_binding    complement(1867..1888)
                     /label=LacO
     misc_feature    1889..1908
                     /label=PCR donor Reverse Primer
     rep_origin      complement(2229..2911)
                     /direction=LEFT
                     /label=ColE1 origin
     CDS             complement(3009..3668)
                     /label=AmpR
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtggcga gggcgatgcc acctacggga
      421 tgcggccgca ccggtggatc cgaattcgct agcccgcggt ctagactcga gggatccgga
      481 gccacgaact tctctctgtt aaagcaagca ggagacgtgg aagaaaaccc cggtcctatg
      541 ccacccaagc gcaaaacgct taacgctgcg gctgaggcta acgctcatgc cgacggacac
      601 gccgacggaa acgccgacgg acacgtggcc aatacggccg cgtcctcgaa taatgcgagg
      661 ttcgctgatc tcactaacat cgatactccg ggtctgggac ccacaactac gaccctgctc
      721 gtggaaccag cacgctcaaa gcgtcaacga gtgtcccgcg catgcgacca gtgccgtgca
      781 gcccgagaga aatgcgacgg aatacagcct gcgtgtttcc cgtgcgtttc ccagggaagg
      841 tcctgcactt atcaggcttc gccgaaaaag aggggagttc aaaccggtta tattcgtacg
      901 ctggagctcg ccctcgcctg gatgtttgaa aatgtcgcgc gttccgaaga tgccttgcat
      961 aacctcctcg tccgtgacgc cggacaagga tcagctctgc tcgttggtaa agattcgccg
     1021 gctgccgagc gactccatgc ccgttgggct actagccgtg tcaataagag cattacccgc
     1081 ctcctccgtc agttggagct ccctcctacc gccacggcta cggcctcgat aatgccgcac
     1141 gtgatggagc agcctctcag taccagcatt aaccccgtca acgaccgctt caacggtatt
     1201 cccaacccca ctccgtataa ctccgatgca gctctcgatg ctatcactca gaccaacgat
     1261 tatggaagcg taaatacaca tggtatcctc tctacttacc cgccaccggc tacgcacctt
     1321 aatgaagctt ccgtcgctct cgctcccggt ggcgcccccc cccgaccgcc tcctccgtat
     1381 gttgacagca cgaccaatca cccgccgtac cactcgaatc tggttccaat ggcgaacttt
     1441 ggttactcga ccgttgatta cgatgccatg gttgacgatt tggctagcat tgaatacacg
     1501 gacgctgtgg atgtcgaccc acagtttatg accaatctgg gattcgttcc tggatgtaac
     1561 ttctccgaca ttaatacata cgaacagtga actagtatcg ataagcttga gctcgtcgac
     1621 atcattctca gtatcccctg cttatgcact caccactttt tcccattatt ttattcatgt
     1681 tgtgttgcta aattcaacta cattcgtatt accatgttgt ttcatttgtt ttttttttat
     1741 ctcagctgtc aaaataaaag cattgaaata caacattgtt ctatttttca ttgtggtttt
     1801 gcatgcccgg taaataaaaa aacaaggtaa taaaaataag atgcgaaatg agaagtatat
     1861 ctgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
     1921 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
     1981 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
     2041 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
     2101 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
     2161 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     2221 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     2281 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     2341 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     2401 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc aatgctcacg ctgtaggtat
     2461 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     2521 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     2581 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     2641 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt
     2701 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     2761 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     2821 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     2881 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
     2941 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
     3001 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
     3061 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
     3121 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
     3181 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
     3241 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
     3301 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
     3361 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
     3421 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
     3481 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
     3541 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
     3601 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
     3661 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
     3721 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
     3781 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
     3841 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
     3901 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
     3961 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc
     4021 atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtc
//
