LOCUS       Exported                3730 bp DNA     circular SYN 12-JUL-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3730)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 13, 2021 from SnapGene Viewer 5.3.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3730
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             complement(239..307)
                     /label=LacZ alpha
     misc_feature    335..354
                     /label=PCR donor Forward Primer
     misc_feature    359..375
                     /label=M13 -40
     primer_bind     378..395
                     /label=M13-fwd
     misc_feature    396..418
                     /label=gBait gRNA
     misc_feature    422..429
                     /label=NotI
     misc_feature    430..435
                     /label=AgeI
     misc_feature    436..441
                     /label=BamHI
     misc_feature    442..447
                     /label=EcoRI
     misc_feature    448..453
                     /label=NheI
     misc_feature    454..459
                     /label=SacII
     misc_feature    460..465
                     /label=XbaI
     misc_feature    466..471
                     /label=XhoI
     misc_feature    472..537
                     /label=p2A peptide from Tol2kit
     misc_feature    538..1254
                     /label=eGFP no start / stop
     misc_feature    1255..1260
                     /label=SpeI
     misc_feature    1261..1266
                     /label=ClaI
     misc_feature    1267..1272
                     /label=HindIII
     misc_feature    1273..1278
                     /label=SacI
     misc_feature    1279..1284
                     /label=SalI
     polyA_signal    1288..1522
                     /label=ubb polyA sequence
     misc_binding    complement(1531..1552)
                     /label=LacO
     misc_feature    1553..1572
                     /label=PCR donor Reverse Primer
     rep_origin      complement(1893..2575)
                     /direction=LEFT
                     /label=ColE1 origin
     CDS             complement(2673..3332)
                     /label=AmpR
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtggcga gggcgatgcc acctacggga
      421 tgcggccgca ccggtggatc cgaattcgct agcccgcggt ctagactcga gggatccgga
      481 gccacgaact tctctctgtt aaagcaagca ggagacgtgg aagaaaaccc cggtcctgtg
      541 agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac
      601 gtaaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac ctacggcaag
      661 ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg
      721 accaccctga cctacggcgt gcagtgcttc agccgctacc ccgaccacat gaagcagcac
      781 gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat cttcttcaag
      841 gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac
      901 cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg
      961 gagtacaact acaacagcca caacgtctat atcatggccg acaagcagaa gaacggcatc
     1021 aaggtgaact tcaagatccg ccacaacatc gaggacggca gcgtgcagct cgccgaccac
     1081 taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg
     1141 agcacccagt ccgccctgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg
     1201 gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa gtaaactagt
     1261 atcgataagc ttgagctcgt cgacatcatt ctcagtatcc cctgcttatg cactcaccac
     1321 tttttcccat tattttattc atgttgtgtt gctaaattca actacattcg tattaccatg
     1381 ttgtttcatt tgtttttttt ttatctcagc tgtcaaaata aaagcattga aatacaacat
     1441 tgttctattt ttcattgtgg ttttgcatgc ccggtaaata aaaaaacaag gtaataaaaa
     1501 taagatgcga aatgagaagt atatctgtga aattgttatc cgctcacaat tccacacaac
     1561 atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca
     1621 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat
     1681 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc
     1741 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
     1801 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
     1861 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     1921 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     1981 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     2041 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     2101 tctcaatgct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     2161 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     2221 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     2281 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     2341 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     2401 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     2461 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     2521 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
     2581 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
     2641 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
     2701 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
     2761 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
     2821 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
     2881 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
     2941 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
     3001 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
     3061 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
     3121 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
     3181 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
     3241 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
     3301 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
     3361 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
     3421 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
     3481 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
     3541 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
     3601 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
     3661 gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg
     3721 ccctttcgtc
//
