LOCUS       Exported                3709 bp DNA     circular SYN 12-JUL-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3709)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 13, 2021 from SnapGene Viewer 5.3.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3709
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             complement(239..307)
                     /label=LacZ alpha
     misc_feature    335..354
                     /label=PCR donor Forward Primer
     misc_feature    359..375
                     /label=M13 -40
     primer_bind     378..395
                     /label=M13-fwd
     misc_feature    396..418
                     /label=gBait gRNA
     misc_feature    422..429
                     /label=NotI
     misc_feature    430..435
                     /label=AgeI
     misc_feature    436..441
                     /label=BamHI
     misc_feature    442..447
                     /label=EcoRI
     misc_feature    448..453
                     /label=NheI
     misc_feature    454..459
                     /label=SacII
     misc_feature    460..465
                     /label=XbaI
     misc_feature    466..471
                     /label=XhoI
     misc_feature    472..537
                     /label=p2A peptide from Tol2kit
     misc_feature    538..1233
                     /label=mScarlet
     misc_feature    1234..1239
                     /label=SpeI
     misc_feature    1240..1245
                     /label=ClaI
     misc_feature    1246..1251
                     /label=HindIII
     misc_feature    1252..1257
                     /label=SacI
     misc_feature    1258..1263
                     /label=SalI
     polyA_signal    1267..1501
                     /label=ubb polyA sequence
     misc_binding    complement(1510..1531)
                     /label=LacO
     misc_feature    1532..1551
                     /label=PCR donor Reverse Primer
     rep_origin      complement(1872..2554)
                     /direction=LEFT
                     /label=ColE1 origin
     CDS             complement(2652..3311)
                     /label=AmpR
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtggcga gggcgatgcc acctacggga
      421 tgcggccgca ccggtggatc cgaattcgct agcccgcggt ctagactcga gggatccgga
      481 gccacgaact tctctctgtt aaagcaagca ggagacgtgg aagaaaaccc cggtcctgtg
      541 agcaagggcg aggcagtgat caaggagttc atgcggttca aggtgcacat ggagggctcc
      601 atgaacggcc acgagttcga gatcgagggc gagggcgagg gccgccccta cgagggcacc
      661 cagaccgcca agctgaaggt gaccaagggt ggccccctgc ccttctcctg ggacatcctg
      721 tcccctcagt tcatgtacgg ctccagggcc ttcaccaagc accccgccga catccccgac
      781 tactataagc agtccttccc cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac
      841 ggcggcgccg tgaccgtgac ccaggacacc tccctggagg acggcaccct gatctacaag
      901 gtgaagctcc gcggcaccaa cttccctcct gacggccccg taatgcagaa gaagacaatg
      961 ggctgggaag cgtccaccga gcggttgtac cccgaggacg gcgtgctgaa gggcgacatt
     1021 aagatggccc tgcgcctgaa ggacggcggc cgctacctgg cggacttcaa gaccacctac
     1081 aaggccaaga agcccgtgca gatgcccggc gcctacaacg tcgaccgcaa gttggacatc
     1141 acctcccaca acgaggacta caccgtggtg gaacagtacg aacgctccga gggccgccac
     1201 tccaccggcg gcatggacga gctgtacaag taaactagta tcgataagct tgagctcgtc
     1261 gacatcattc tcagtatccc ctgcttatgc actcaccact ttttcccatt attttattca
     1321 tgttgtgttg ctaaattcaa ctacattcgt attaccatgt tgtttcattt gttttttttt
     1381 tatctcagct gtcaaaataa aagcattgaa atacaacatt gttctatttt tcattgtggt
     1441 tttgcatgcc cggtaaataa aaaaacaagg taataaaaat aagatgcgaa atgagaagta
     1501 tatctgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag
     1561 tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg
     1621 cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg
     1681 gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc
     1741 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc
     1801 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
     1861 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
     1921 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
     1981 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
     2041 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcaatgctc acgctgtagg
     2101 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
     2161 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
     2221 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
     2281 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt
     2341 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
     2401 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
     2461 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
     2521 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
     2581 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
     2641 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
     2701 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
     2761 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca
     2821 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
     2881 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
     2941 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg
     3001 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
     3061 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
     3121 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
     3181 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
     3241 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta
     3301 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
     3361 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
     3421 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
     3481 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt
     3541 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
     3601 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga aaccattatt
     3661 atcatgacat taacctataa aaataggcgt atcacgaggc cctttcgtc
//
