LOCUS       Exported                3853 bp DNA     circular SYN 12-JUL-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3853)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 13, 2021 from SnapGene Viewer 5.3.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3853
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             complement(239..307)
                     /label=LacZ alpha
     misc_feature    335..354
                     /label=PCR donor Forward Primer
     misc_feature    359..375
                     /label=M13 -40
     primer_bind     378..395
                     /label=M13-fwd
     misc_feature    396..418
                     /label=gBait gRNA
     misc_feature    422..429
                     /label=NotI
     misc_feature    430..435
                     /label=AgeI
     misc_feature    436..441
                     /label=BamHI
     misc_feature    442..447
                     /label=EcoRI
     misc_feature    448..453
                     /label=NheI
     misc_feature    454..459
                     /label=SacII
     misc_feature    460..465
                     /label=XbaI
     misc_feature    466..471
                     /label=XhoI
     misc_feature    472..537
                     /label=p2A peptide
     misc_feature    538..1251
                     /label=Venus
     misc_feature    1255..1377
                     /label=PEST
     misc_feature    1378..1383
                     /label=SpeI
     misc_feature    1384..1389
                     /label=ClaI
     misc_feature    1390..1395
                     /label=HindIII
     misc_feature    1396..1401
                     /label=SacI
     misc_feature    1402..1407
                     /label=SalI
     polyA_signal    1411..1645
                     /label=ubb polyA sequence
     misc_binding    complement(1654..1675)
                     /label=LacO
     misc_feature    1676..1695
                     /label=PCR donor Reverse Primer
     rep_origin      complement(2016..2698)
                     /direction=LEFT
                     /label=ColE1 origin
     CDS             complement(2796..3455)
                     /label=AmpR
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtggcga gggcgatgcc acctacggga
      421 tgcggccgca ccggtggatc cgaattcgct agcccgcggt ctagactcga gggatccgga
      481 gccacgaact tctctctgtt aaagcaagca ggagacgtgg aagaaaaccc cggtcctgtg
      541 agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac
      601 gtaaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac ctacggcaag
      661 ctgaccctga agctgatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg
      721 accaccctgg gctacggcct gcagtgcttc gcccgctacc ccgaccacat gaagcagcac
      781 gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat cttcttcaag
      841 gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac
      901 cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg
      961 gagtacaact acaacagcca caacgtctat atcaccgccg acaagcagaa gaacggcatc
     1021 aaggccaact tcaagatccg ccacaacatc gaggacggcg gcgtgcagct cgccgaccac
     1081 taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg
     1141 agctaccagt ccgccctgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg
     1201 gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa gcttagccat
     1261 ggcttcccgc cggcggtggc ggcgcaggat gatggcacgc tgcccatgtc ttgtgcccag
     1321 gagagcggga tggaccgtca ccctgcagcc tgtgcttctg ctaggatcaa tgtgtagact
     1381 agtatcgata agcttgagct cgtcgacatc attctcagta tcccctgctt atgcactcac
     1441 cactttttcc cattatttta ttcatgttgt gttgctaaat tcaactacat tcgtattacc
     1501 atgttgtttc atttgttttt tttttatctc agctgtcaaa ataaaagcat tgaaatacaa
     1561 cattgttcta tttttcattg tggttttgca tgcccggtaa ataaaaaaac aaggtaataa
     1621 aaataagatg cgaaatgaga agtatatctg tgaaattgtt atccgctcac aattccacac
     1681 aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc
     1741 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg
     1801 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct
     1861 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
     1921 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
     1981 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
     2041 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
     2101 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
     2161 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
     2221 ctttctcaat gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
     2281 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
     2341 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
     2401 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
     2461 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
     2521 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
     2581 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
     2641 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
     2701 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
     2761 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
     2821 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
     2881 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
     2941 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
     3001 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
     3061 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
     3121 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
     3181 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
     3241 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
     3301 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
     3361 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
     3421 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
     3481 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
     3541 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
     3601 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
     3661 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
     3721 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
     3781 cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg
     3841 aggccctttc gtc
//
