LOCUS PE_pRNA_Origin_Fragment_evopreq1-1_trimmed 3041 bp DNA circular UNA 16-OCT-2020 DEFINITION pFYF1320 withBsmBI spacer insertion CACC AGAG GTCC CGCG. ACCESSION urn.local...m-cqqsrv7 VERSION urn.local...m-cqqsrv7 KEYWORDS . SOURCE ORGANISM . FEATURES Location/Qualifiers regulatory 67..330 /note="Geneious type: promoter" /standard_name="hU6" regulatory 338..373 /note="Geneious type: terminator" /standard_name="bidirectional terminator, weaker in forward direction" misc_feature 339..372 /standard_name="Chen, Voigt et al. strength <53 (for full terminator)" misc_feature complement(339..371) /standard_name="Chen, Voigt et al. strength ~4.3±1.3 (almost full sequence characterized)" misc_feature 340..347 /standard_name="A-tract" misc_feature 348..363 /standard_name="hairpin" misc_feature 354..357 /standard_name="loop" misc_feature complement(364..371) /standard_name="A-tract" CDS complement(388..1065) /standard_name="mRFP" misc_feature complement(1066..1090) /standard_name="RBS" misc_feature complement(1091..1144) /standard_name="pTet" misc_feature complement(1094..1144) /standard_name="BBa_R0040" misc_feature complement(1097..1102) /standard_name="-10?" misc_feature complement(1101..1119) /standard_name="tetO" misc_feature complement(1118..1139) /standard_name misc_feature complement(1120..1125) /standard_name="-35?" primer_bind complement(1120..1174) /standard_name="evopreq1-1 reverse" misc_feature complement(1126..1144) /standard_name="tetO" misc_RNA 1152..1188 /standard_name="evopreq1-1 trimmed" primer_bind 1165..1232 /standard_name="evopreq1-1trimmedfp" regulatory 1189..1195 /note="Geneious type: terminator" /standard_name="U6 terminator" misc_feature complement(1277..1896) /note="Geneious type: origin of replication" /standard_name="pBR322 origin" CDS complement(2051..2911) /standard_name="amp marker" primer_bind complement(2173..2202) /standard_name="LoAS0038" primer_bind 2192..2214 /standard_name="LoAS0037" misc_feature 2192..2202 /standard_name="USER" misc_feature 2193..2195 /standard_name="GAC > GAT to remove Bsa1 Site" regulatory complement(2953..2981) /note="Geneious type: promoter" /standard_name="amp prom" ORIGIN 1 gacgtcgcta gctgtacaaa aaagcaggct ttaaaggaac caattcagtc gactggatcc 61 ggtaccaagg tcgggcagga agagggccta tttcccatga ttccttcata tttgcatata 121 cgatacaagg ctgttagaga gataattaga attaatttga ctgtaaacac aaagatatta 181 gtacaaaata cgtgacgtag aaagtaataa tttcttgggt agtttgcagt tttaaaatta 241 tgttttaaaa tggactatca tatgcttacc gtaacttgaa agtatttcga tttcttggct 301 ttatatatct tgtggaaagg acgaaacacc cgagaccgta gtcaaaagcc tccggtcgga 361 ggcttttgac ttggctgagg agtgccatta agcaccggtg gagtgacgac cttcagcacg 421 ttcgtactgt tcaacgatgg tgtagtcttc gttgtgggag gtgatgtcca gtttgatgtc 481 ggttttgtaa gcacccggca gctgaaccgg ttttttagcc atgtaggtgg ttttaacttc 541 agcgtcgtag tgaccaccgt ctttcagttt cagacgcatt ttgatttcac ctttcagagc 601 accgtcttcc gggtacatac gttcggtgga agcttcccaa cccatggttt ttttctgcat 661 aaccggaccg tcggacggga agttggtacc acgcagttta actttgtaga tgaactcacc 721 gtcttgcagg gaggagtcct gggtaacggt aacaacacca ccgtcttcga agttcataac 781 acgttcccat ttgaaacctt ccgggaagga cagtttcagg tagtccggga tgtcagccgg 841 gtgtttaacg taagctttgg aaccgtactg gaactgcggg gacaggatgt cccaagcgaa 901 cggcagcgga ccacctttgg taactttcag tttagcggtc tgggtacctt cgtacggacg 961 accttcacct tcaccttcga tttcgaactc gtgaccgtta acggaacctt ccatacgaac 1021 tttgaaacgc atgaactctt tgataacgtc ttcgctactc gccatagatc ctttctcctc 1081 tttcagatcc gtgctcagta tctctatcac tgatagggat gtcaatctct atcactgata 1141 gggaggtctc gcgcggttct atctagttac gcgttaaacc aactagaatt tttttaagct 1201 tgggccgctc gaggtacctc tctacatatg acatgtgagc aaaaggccag caaaaggcca 1261 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc 1321 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc 1381 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg 1441 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta 1501 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg 1561 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac 1621 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag 1681 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat 1741 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat 1801 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc 1861 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt 1921 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct 1981 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt 2041 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc 2101 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac 2161 catctggccc cagtgctgca atgataccgc gagatccacg ctcaccggct ccagatttat 2221 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg 2281 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata 2341 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta 2401 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt 2461 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag 2521 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa 2581 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc 2641 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt 2701 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc 2761 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta 2821 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa 2881 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca 2941 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac 3001 aaataggggt tccgcgcaca tttccccgaa aagtgccacc t //