LOCUS       PE_pRNA_Origin_Fragment_evopreq1-1_trimmed        3041 bp    DNA     circular UNA 16-OCT-2020
DEFINITION  pFYF1320 withBsmBI spacer insertion CACC AGAG GTCC CGCG.
ACCESSION   urn.local...m-cqqsrv7
VERSION     urn.local...m-cqqsrv7
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     regulatory      67..330
                     /note="Geneious type: promoter"
                     /standard_name="hU6"
     regulatory      338..373
                     /note="Geneious type: terminator"
                     /standard_name="bidirectional terminator, weaker in
                     forward direction"
     misc_feature    339..372
                     /standard_name="Chen, Voigt et al. strength <53 (for full
                     terminator)"
     misc_feature    complement(339..371)
                     /standard_name="Chen, Voigt et al. strength ~4.3±1.3
                     (almost full sequence characterized)"
     misc_feature    340..347
                     /standard_name="A-tract"
     misc_feature    348..363
                     /standard_name="hairpin"
     misc_feature    354..357
                     /standard_name="loop"
     misc_feature    complement(364..371)
                     /standard_name="A-tract"
     CDS             complement(388..1065)
                     /standard_name="mRFP"
     misc_feature    complement(1066..1090)
                     /standard_name="RBS"
     misc_feature    complement(1091..1144)
                     /standard_name="pTet"
     misc_feature    complement(1094..1144)
                     /standard_name="BBa_R0040"
     misc_feature    complement(1097..1102)
                     /standard_name="-10?"
     misc_feature    complement(1101..1119)
                     /standard_name="tetO"
     misc_feature    complement(1118..1139)
                     /standard_name
     misc_feature    complement(1120..1125)
                     /standard_name="-35?"
     primer_bind     complement(1120..1174)
                     /standard_name="evopreq1-1 reverse"
     misc_feature    complement(1126..1144)
                     /standard_name="tetO"
     misc_RNA        1152..1188
                     /standard_name="evopreq1-1 trimmed"
     primer_bind     1165..1232
                     /standard_name="evopreq1-1trimmedfp"
     regulatory      1189..1195
                     /note="Geneious type: terminator"
                     /standard_name="U6 terminator"
     misc_feature    complement(1277..1896)
                     /note="Geneious type: origin of replication"
                     /standard_name="pBR322 origin"
     CDS             complement(2051..2911)
                     /standard_name="amp marker"
     primer_bind     complement(2173..2202)
                     /standard_name="LoAS0038"
     primer_bind     2192..2214
                     /standard_name="LoAS0037"
     misc_feature    2192..2202
                     /standard_name="USER"
     misc_feature    2193..2195
                     /standard_name="GAC > GAT to remove Bsa1 Site"
     regulatory      complement(2953..2981)
                     /note="Geneious type: promoter"
                     /standard_name="amp prom"
ORIGIN      
        1 gacgtcgcta gctgtacaaa aaagcaggct ttaaaggaac caattcagtc gactggatcc
       61 ggtaccaagg tcgggcagga agagggccta tttcccatga ttccttcata tttgcatata
      121 cgatacaagg ctgttagaga gataattaga attaatttga ctgtaaacac aaagatatta
      181 gtacaaaata cgtgacgtag aaagtaataa tttcttgggt agtttgcagt tttaaaatta
      241 tgttttaaaa tggactatca tatgcttacc gtaacttgaa agtatttcga tttcttggct
      301 ttatatatct tgtggaaagg acgaaacacc cgagaccgta gtcaaaagcc tccggtcgga
      361 ggcttttgac ttggctgagg agtgccatta agcaccggtg gagtgacgac cttcagcacg
      421 ttcgtactgt tcaacgatgg tgtagtcttc gttgtgggag gtgatgtcca gtttgatgtc
      481 ggttttgtaa gcacccggca gctgaaccgg ttttttagcc atgtaggtgg ttttaacttc
      541 agcgtcgtag tgaccaccgt ctttcagttt cagacgcatt ttgatttcac ctttcagagc
      601 accgtcttcc gggtacatac gttcggtgga agcttcccaa cccatggttt ttttctgcat
      661 aaccggaccg tcggacggga agttggtacc acgcagttta actttgtaga tgaactcacc
      721 gtcttgcagg gaggagtcct gggtaacggt aacaacacca ccgtcttcga agttcataac
      781 acgttcccat ttgaaacctt ccgggaagga cagtttcagg tagtccggga tgtcagccgg
      841 gtgtttaacg taagctttgg aaccgtactg gaactgcggg gacaggatgt cccaagcgaa
      901 cggcagcgga ccacctttgg taactttcag tttagcggtc tgggtacctt cgtacggacg
      961 accttcacct tcaccttcga tttcgaactc gtgaccgtta acggaacctt ccatacgaac
     1021 tttgaaacgc atgaactctt tgataacgtc ttcgctactc gccatagatc ctttctcctc
     1081 tttcagatcc gtgctcagta tctctatcac tgatagggat gtcaatctct atcactgata
     1141 gggaggtctc gcgcggttct atctagttac gcgttaaacc aactagaatt tttttaagct
     1201 tgggccgctc gaggtacctc tctacatatg acatgtgagc aaaaggccag caaaaggcca
     1261 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
     1321 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     1381 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
     1441 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
     1501 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
     1561 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     1621 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
     1681 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat
     1741 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
     1801 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
     1861 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
     1921 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
     1981 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
     2041 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
     2101 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
     2161 catctggccc cagtgctgca atgataccgc gagatccacg ctcaccggct ccagatttat
     2221 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
     2281 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
     2341 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta
     2401 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
     2461 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
     2521 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
     2581 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
     2641 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt
     2701 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
     2761 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
     2821 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
     2881 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
     2941 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
     3001 aaataggggt tccgcgcaca tttccccgaa aagtgccacc t
//
