LOCUS       pCS2_SaCas9             7425 bp ds-DNA     circular     17-APR-2018
DEFINITION  .
ACCESSION   -                                                                  
SOURCE      -                                                                  
FEATURES             Location/Qualifiers
     misc_feature    2621..3416
                     /label="Sequenced DNA"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     regulatory      100..103
                     /label="vertebrate consensus sequence for stronginitiation  of translation (Kozak, 1987)"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    3559..3577
                     /label="SV40R"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     CDS             151..3306
                     /label="SaCas9"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     CDS             106..126
                     /label="SV40 NLS"
                     /ApEinfo_revcolor=#ff0000
                     /ApEinfo_fwdcolor=#ff0000
     CDS             3307..3354
                     /label="nucleoplasmin NLS"
                     /ApEinfo_revcolor=#ff0000
                     /ApEinfo_fwdcolor=#ff0000
     mRNA            1..1
                     /label="mRNA"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    109..769
                     /label="Sequenced DNA"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     CDS             3361..3441
                     /label="3xHA"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     Primer          35..52
                     /label="SP6"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
ORIGIN
        1 CGCCATTCTG CCTGGGGACG TCGGAGCAAG CTTGATTTAG GTGACACTAT AGAATACAAG
       61 CTACTTGTTC TTTTTGCAGG ATCCCATCGA TTCGAATTCa tggccccaaa gaagaagcgg
      121 aaggtcggta tccacggagt cccagcagcc aagcggaact acatcctggg cctggacatc
      181 ggcatcacca gcgtgggcta cggcatcatc gactacgaga cacgggacgt gatcgatgcc
      241 ggcgtgcggc tgttcaaaga ggccaacgtg gaaaacaacg agggcaggcg gagcaagaga
      301 ggcgccagaa ggctgaagcg gcggaggcgg catagaatcc agagagtgaa gaagctgctg
      361 ttcgactaca acctgctgac cgaccacagc gagctgagcg gcatcaaccc ctacgaggcc
      421 agagtgaagg gcctgagcca gaagctgagc gaggaagagt tctctgccgc cctgctgcac
      481 ctggccaaga gaagaggcgt gcacaacgtg aacgaggtgg aagaggacac cggcaacgag
      541 ctgtccacca aagagcagat cagccggaac agcaaggccc tggaagagaa atacgtggcc
      601 gaactgcagc tggaacggct gaagaaagac ggcgaagtgc ggggcagcat caacagattc
      661 aagaccagcg actacgtgaa agaagccaaa cagctgctga aggtgcagaa ggcctaccac
      721 cagctggacc agagcttcat cgacacctac atcgacctgc tggaaacccg gcggacctac
      781 tatgagggac ctggcgaggg cagccccttc ggctggaagg acatcaaaga atggtacgag
      841 atgctgatgg gccactgcac ctacttcccc gaggaactgc ggagcgtgaa gtacgcctac
      901 aacgccgacc tgtacaacgc cctgaacgac ctgaacaatc tcgtgatcac cagggacgag
      961 aacgagaagc tggaatatta cgagaagttc cagatcatcg agaacgtgtt caagcagaag
     1021 aagaagccca ccctgaagca gatcgccaaa gaaatcctcg tgaacgaaga ggatattaag
     1081 ggctacagag tgaccagcac cggcaagccc gagttcacca acctgaaggt gtaccacgac
     1141 atcaaggaca ttaccgcccg gaaagagatt attgagaacg ccgagctgct ggatcagatt
     1201 gccaagatcc tgaccatcta ccagagcagc gaggacatcc aggaagaact gaccaatctg
     1261 aactccgagc tgacccagga agagatcgag cagatctcta atctgaaggg ctataccggc
     1321 acccacaacc tgagcctgaa ggccatcaac ctgatcctgg acgagctgtg gcacaccaac
     1381 gacaaccaga tcgctatctt caaccggctg aagctggtgc ccaagaaggt ggacctgtcc
     1441 cagcagaaag agatccccac caccctggtg gacgacttca tcctgagccc cgtcgtgaag
     1501 agaagcttca tccagagcat caaagtgatc aacgccatca tcaagaagta cggcctgccc
     1561 aacgacatca ttatcgagct ggcccgcgag aagaactcca aggacgccca gaaaatgatc
     1621 aacgagatgc agaagcggaa ccggcagacc aacgagcgga tcgaggaaat catccggacc
     1681 accggcaaag agaacgccaa gtacctgatc gagaagatca agctgcacga catgcaggaa
     1741 ggcaagtgcc tgtacagcct ggaagccatc cctctggaag atctgctgaa caaccccttc
     1801 aactatgagg tggaccacat catccccaga agcgtgtcct tcgacaacag cttcaacaac
     1861 aaggtgctcg tgaagcagga agaaaacagc aagaagggca accggacccc attccagtac
     1921 ctgagcagca gcgacagcaa gatcagctac gaaaccttca agaagcacat cctgaatctg
     1981 gccaagggca agggcagaat cagcaagacc aagaaagagt atctgctgga agaacgggac
     2041 atcaacaggt tctccgtgca gaaagacttc atcaaccgga acctggtgga taccagatac
     2101 gccaccagag gcctgatgaa cctgctgcgg agctacttca gagtgaacaa cctggacgtg
     2161 aaagtgaagt ccatcaatgg cggcttcacc agctttctgc ggcggaagtg gaagtttaag
     2221 aaagagcgga acaaggggta caagcaccac gccgaggacg ccctgatcat tgccaacgcc
     2281 gatttcatct tcaaagagtg gaagaaactg gacaaggcca aaaaagtgat ggaaaaccag
     2341 atgttcgagg aaaagcaggc cgagagcatg cccgagatcg aaaccgagca ggagtacaaa
     2401 gagatcttca tcacccccca ccagatcaag cacattaagg acttcaagga ctacaagtac
     2461 agccaccggg tggacaagaa gcctaataga gagctgatta acgacaccct gtactccacc
     2521 cggaaggacg acaagggcaa caccctgatc gtgaacaatc tgaacggcct gtacgacaag
     2581 gacaatgaca agctgaaaaa gctgatcaac aagagccccg aaaagctgct gatgtaccac
     2641 cacgaccccc agacctacca gaaactgaag ctgattatgg aacagtacgg cgacgagaag
     2701 aatcccctgt acaagtacta cgaggaaacc gggaactacc tgaccaagta ctccaaaaag
     2761 gacaacggcc ccgtgatcaa gaagattaag tattacggca acaaactgaa cgcccatctg
     2821 gacatcaccg acgactaccc caacagcaga aacaaggtcg tgaagctgtc cctgaagccc
     2881 tacagattcg acgtgtacct ggacaatggc gtgtacaagt tcgtgaccgt gaagaatctg
     2941 gatgtgatca aaaaagaaaa ctactacgaa gtgaatagca agtgctatga ggaagctaag
     3001 aagctgaaga agatcagcaa ccaggccgag tttatcgcct ccttctacaa caacgatctg
     3061 atcaagatca acggcgagct gtatagagtg atcggcgtga acaacgacct gctgaaccgg
     3121 atcgaagtga acatgatcga catcacctac cgcgagtacc tggaaaacat gaacgacaag
     3181 aggcccccca ggatcattaa gacaatcgcc tccaagaccc agagcattaa gaagtacagc
     3241 acagacattc tgggcaacct gtatgaagtg aaatctaaga agcaccctca gatcatcaaa
     3301 aagggcaaaa ggccggcggc cacgaaaaag gccggccagg caaaaaagaa aaagggatcc
     3361 tacccatacg atgttccaga ttacgcttac ccatacgatg ttccagatta cgcttaccca
     3421 tacgatgttc cagattacgc ttaaTCTAGA ACTATAGTGA GTCGTATTAC GTAGATCCAG
     3481 ACATGATAAG ATACATTGAT GAGTTTGGAC AAACCACAAC TAGAATGCAG TGAAAAAAAT
     3541 GCTTTATTTG TGAAATTTGT GATGCTATTG CTTTATTTGT AACCATTATA AGCTGCAATA
     3601 AACAAGTTAA CAACAACAAT TGCATTCATT TTATGTTTCA GGTTCAGGGG GAGGTGTGGG
     3661 AGGTTTTTTA ATTCGCGGCC GCGGCGCCAA TGCATTGGGC CCGGTACCCA GCTTTTGTTC
     3721 CCTTTAGTGA GGGTTAATTG CGCGCTTGGC GTAATCATGG TCATAGCTGT TTCCTGTGTG
     3781 AAATTGTTAT CCGCTCACAA TTCCACACAA CATACGAGCC GGAAGCATAA AGTGTAAAGC
     3841 CTGGGGTGCC TAATGAGTGA GCTAACTCAC ATTAATTGCG TTGCGCTCAC TGCCCGCTTT
     3901 CCAGTCGGGA AACCTGTCGT GCCAGCTGCA TTAATGAATC GGCCAACGCG CGGGGAGAGG
     3961 CGGTTTGCGT ATTGGGCGCT CTTCCGCTTC CTCGCTCACT GACTCGCTGC GCTCGGTCGT
     4021 TCGGCTGCGG CGAGCGGTAT CAGCTCACTC AAAGGCGGTA ATACGGTTAT CCACAGAATC
     4081 AGGGGATAAC GCAGGAAAGA ACATGTGAGC AAAAGGCCAG CAAAAGGCCA GGAACCGTAA
     4141 AAAGGCCGCG TTGCTGGCGT TTTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA
     4201 TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC
     4261 CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC
     4321 CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA GGTATCTCAG
     4381 TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA
     4441 CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC
     4501 GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC
     4561 AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA AGGACAGTAT TTGGTATCTG
     4621 CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA
     4681 AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA
     4741 AGGATCTCAA GAAGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA
     4801 CTCACGTTAA GGGATTTTGG TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT
     4861 AAATTAAAAA TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAAACTT GGTCTGACAG
     4921 TTACCAATGC TTAATCAGTG AGGCACCTAT CTCAGCGATC TGTCTATTTC GTTCATCCAT
     4981 AGTTGCCTGA CTCCCCGTCG TGTAGATAAC TACGATACGG GAGGGCTTAC CATCTGGCCC
     5041 CAGTGCTGCA ATGATACCGC GAGACCCACG CTCACCGGCT CCAGATTTAT CAGCAATAAA
     5101 CCAGCCAGCC GGAAGGGCCG AGCGCAGAAG TGGTCCTGCA ACTTTATCCG CCTCCATCCA
     5161 GTCTATTAAT TGTTGCCGGG AAGCTAGAGT AAGTAGTTCG CCAGTTAATA GTTTGCGCAA
     5221 CGTTGTTGCC ATTGCTACAG GCATCGTGGT GTCACGCTCG TCGTTTGGTA TGGCTTCATT
     5281 CAGCTCCGGT TCCCAACGAT CAAGGCGAGT TACATGATCC CCCATGTTGT GCAAAAAAGC
     5341 GGTTAGCTCC TTCGGTCCTC CGATCGTTGT CAGAAGTAAG TTGGCCGCAG TGTTATCACT
     5401 CATGGTTATG GCAGCACTGC ATAATTCTCT TACTGTCATG CCATCCGTAA GATGCTTTTC
     5461 TGTGACTGGT GAGTACTCAA CCAAGTCATT CTGAGAATAG TGTATGCGGC GACCGAGTTG
     5521 CTCTTGCCCG GCGTCAATAC GGGATAATAC CGCGCCACAT AGCAGAACTT TAAAAGTGCT
     5581 CATCATTGGA AAACGTTCTT CGGGGCGAAA ACTCTCAAGG ATCTTACCGC TGTTGAGATC
     5641 CAGTTCGATG TAACCCACTC GTGCACCCAA CTGATCTTCA GCATCTTTTA CTTTCACCAG
     5701 CGTTTCTGGG TGAGCAAAAA CAGGAAGGCA AAATGCCGCA AAAAAGGGAA TAAGGGCGAC
     5761 ACGGAAATGT TGAATACTCA TACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG
     5821 TTATTGTCTC ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT
     5881 TCCGCGCACA TTTCCCCGAA AAGTGCCACC TAAATTGTAA GCGTTAATAT TTTGTTAAAA
     5941 TTCGCGTTAA ATTTTTGTTA AATCAGCTCA TTTTTTAACC AATAGGCCGA AATCGGCAAA
     6001 ATCCCTTATA AATCAAAAGA ATAGACCGAG ATAGGGTTGA GTGTTGTTCC AGTTTGGAAC
     6061 AAGAGTCCAC TATTAAAGAA CGTGGACTCC AACGTCAAAG GGCGAAAAAC CGTCTATCAG
     6121 GGCGATGGCC CACTACGTGA ACCATCACCC TAATCAAGTT TTTTGGGGTC GAGGTGCCGT
     6181 AAAGCACTAA ATCGGAACCC TAAAGGGAGC CCCCGATTTA GAGCTTGACG GGGAAAGCCG
     6241 GCGAACGTGG CGAGAAAGGA AGGGAAGAAA GCGAAAGGAG CGGGCGCTAG GGCGCTGGCA
     6301 AGTGTAGCGG TCACGCTGCG CGTAACCACC ACACCCGCCG CGCTTAATGC GCCGCTACAG
     6361 GGCGCGTCCC ATTCGCCATT CAGGCTGCGC AACTGTTGGG AAGGGCGATC GGTGCGGGCC
     6421 TCTTCGCTAT TACGCCAGTC GACCATAGCC AATTCAATAT GGCGTATATG GACTCATGCC
     6481 AATTCAATAT GGTGGATCTG GACCTGTGCC AATTCAATAT GGCGTATATG GACTCGTGCC
     6541 AATTCAATAT GGTGGATCTG GACCCCAGCC AATTCAATAT GGCGGACTTG GCACCATGCC
     6601 AATTCAATAT GGCGGACTTG GCACTGTGCC AACTGGGGAG GGGTCTACTT GGCACGGTGC
     6661 CAAGTTTGAG GAGGGGTCTT GGCCCTGTGC CAAGTCCGCC ATATTGAATT GGCATGGTGC
     6721 CAATAATGGC GGCCATATTG GCTATATGCC AGGATCAATA TATAGGCAAT ATCCAATATG
     6781 GCCCTATGCC AATATGGCTA TTGGCCAGGT TCAATACTAT GTATTGGCCC TATGCCATAT
     6841 AGTATTCCAT ATATGGGTTT TCCTATTGAC GTAGATAGCC CCTCCCAATG GGCGGTCCCA
     6901 TATACCATAT ATGGGGCTTC CTAATACCGC CCATAGCCAC TCCCCCATTG ACGTCAATGG
     6961 TCTCTATATA TGGTCTTTCC TATTGACGTC ATATGGGCGG TCCTATTGAC GTATATGGCG
     7021 CCTCCCCCAT TGACGTCAAT TACGGTAAAT GGCCCGCCTG GCTCAATGCC CATTGACGTC
     7081 AATAGGACCA CCCACCATTG ACGTCAATGG GATGGCTCAT TGCCCATTCA TATCCGTTCT
     7141 CACGCCCCCT ATTGACGTCA ATGACGGTAA ATGGCCCACT TGGCAGTACA TCAATATCTA
     7201 TTAATAGTAA CTTGGCAAGT ACATTACTAT TGGAAGGACG CCAGGGTACA TTGGCAGTAC
     7261 TCCCATTGAC GTCAATGGCG GTAAATGGCC CGCGATGGCT GCCAAGTACA TCCCCATTGA
     7321 CGTCAATGGG GAGGGGCAAT GACGCAAATG GGCGTTCCAT TGACGTAAAT GGGCGGTAGG
     7381 CGTGCCTAAT GGGAGGTCTA TATAAGCAAT GCTCGTTTAG GGAAC
//