LOCUS       pJL1                    9353 bp ds-DNA     circular     15-JUL-2021
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    pNH603
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7119)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported 2012. jul. 31. from SnapGene Viewer 1.2.0
            http://www.snapgene.com
COMMENT     
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     5'UTR           7..698
                     /note="LEU2 5'UTR"
                     /note="color: #a6acb3"
                     /locus_tag="LEU2 5'UTR"
                     /label="LEU2 5'UTR"
                     /ApEinfo_label="LEU2 5'UTR"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     promoter        276..683
                     /gene="S. cerevisiae LEU2"
                     /note="LEU2 promoter"
                     /note="color: #ffffff; direction: RIGHT"
                     /locus_tag="S. cerevisiae LEU2"
                     /label="S. cerevisiae LEU2"
                     /ApEinfo_label="S. cerevisiae LEU2"
                     /ApEinfo_fwdcolor="#00ffff"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             3530..3535
                     /gene="(null)"
                     /SECDrawAs="Gene"
                     /locus_tag="97-4_WT"
                     /label="97-4_WT"
                     /ApEinfo_label="97-4_WT"
                     /ApEinfo_fwdcolor="#34ff00"
                     /ApEinfo_revcolor="#34ff00"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             1199..2296
                     /codon_start=1
                     /note="LEU2"
                     /note="color: #993366"
                     /translation="MAVTKTIVVLPGDHVGQEITEEAIKVLNAIQECRPDKVNFKFDH
                     H
                     LIGGAAIDATGVPLPDEALEASKKADAVLLGAVGGPKWGTGAVRPEQGLLKIRKELQL
                     Y
                     ANLRPCNFASDSLLDLSPLKPEIARGTDFVVVRELVGGIYFGERKEDEGDGVAWDSEK
                     Y
                     SVPEVQRITRMAAFMALQHNPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPQLTVQ
                     H
                     QLIDSAAMILVKNPTHLNGIIITNNMFGDIISDEASVIPGSLGLLPSASLASLPDKNT
                     A
                     FGLYEPCHGSAPDLPKGKVNPVATILSAAMMLKLSLDLFEEGEIIEQAVKKVLDSGIR
                     T ADLKGTNSTTEVGDAVAKAVRELLA"
                     /locus_tag="LEU2"
                     /label="LEU2"
                     /ApEinfo_label="LEU2"
                     /ApEinfo_fwdcolor="#ff8080"
                     /ApEinfo_revcolor="#ffff80"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    3479..3529
                     /locus_tag="17aa GS linker"
                     /label="17aa GS linker"
                     /ApEinfo_label="17aa GS linker"
                     /ApEinfo_fwdcolor="#ff00c6"
                     /ApEinfo_revcolor="#ff00c6"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     terminator      4636..5330
                     /note="C.albicans ADH1 terminator"
                     /note="color: #ffffff"
                     /locus_tag="C.albicans ADH1 terminator"
                     /label="C.albicans ADH1 terminator"
                     /ApEinfo_label="C.albicans ADH1 terminator"
                     /ApEinfo_fwdcolor="#ff0000"
                     /ApEinfo_revcolor="#ff8000"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     3'UTR           5331..5856
                     /note="LEU2 3'UTR"
                     /note="color: #a6acb3"
                     /locus_tag="LEU2 3'UTR"
                     /label="LEU2 3'UTR"
                     /ApEinfo_label="LEU2 3'UTR"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     promoter        complement(5901..5919)
                     /note="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /note="color: #ffffff; direction: LEFT"
                     /locus_tag="T7 promoter"
                     /label="T7 promoter"
                     /ApEinfo_label="T7 promoter"
                     /ApEinfo_fwdcolor="#00ffff"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    3225..3232
                     /locus_tag="SbfI"
                     /label="SbfI"
                     /ApEinfo_label="SbfI"
                     /ApEinfo_fwdcolor="#ff0012"
                     /ApEinfo_revcolor="#ff0012"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    4613..4615
                     /locus_tag="STOP"
                     /label="STOP"
                     /ApEinfo_label="STOP"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="cyan"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_signal     3233..3469
                     /gene="VP16"
                     /SECDrawAs="Label"
                     /locus_tag="VP16"
                     /label="VP16"
                     /ApEinfo_label="VP16"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     complement(5926..5942)
                     /note="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /note="color: #a020f0; direction: LEFT"
                     /locus_tag="M13 fwd"
                     /label="M13 fwd"
                     /ApEinfo_label="M13 fwd"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_signal     2621..2692
                     /gene="UASg'"
                     /SECDrawAs="Label"
                     /locus_tag="UASg'"
                     /label="UASg'"
                     /ApEinfo_label="UASg'"
                     /ApEinfo_fwdcolor="#00ff2c"
                     /ApEinfo_revcolor="#00ff2c"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_signal     3215..3223
                     /gene="GSGS-PDZ"
                     /SECDrawAs="Label"
                     /locus_tag="GSGS"
                     /label="GSGS"
                     /ApEinfo_label="GSGS"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             3233..3469
                     /gene="VP16"
                     /SECDrawAs="Gene"
                     /locus_tag="VP16(1)"
                     /label="VP16(1)"
                     /ApEinfo_label="VP16"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     rep_origin      6083..6538
                     /direction=RIGHT
                     /note="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /note="color: #ffff00"
                     /locus_tag="f1 ori"
                     /label="f1 ori"
                     /ApEinfo_label="f1 ori"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     promoter        7176..7280
                     /gene="bla"
                     /note="AmpR promoter"
                     /note="color: #ffffff; direction: RIGHT"
                     /locus_tag="bla"
                     /label="bla"
                     /ApEinfo_label="bla"
                     /ApEinfo_fwdcolor="#00ffff"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    3470..3477
                     /locus_tag="NotI"
                     /label="NotI"
                     /ApEinfo_label="NotI"
                     /ApEinfo_fwdcolor="#ff001b"
                     /ApEinfo_revcolor="#ff001b"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2617..3073
                     /gene="Gal1*prom'"
                     /SECDrawAs="Region"
                     /locus_tag="Gal1*prom'"
                     /label="Gal1*prom'"
                     /ApEinfo_label="Gal1*prom'"
                     /ApEinfo_fwdcolor="#00fff9"
                     /ApEinfo_revcolor="#00fff9"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             7281..8141
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin,
                     and related antibiotics"
                     /note="This feature has 2 segments:
                     ÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂ ÃÂÃÂÃÂÃÂÃÂÃÂÃ
                     ÂÃÂ ÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂ 1:ÃÂÃÂÃÂÃÂ
                     ÃÂÃÂÃÂÃÂ 5294ÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂ ..ÃÂ
                     ÃÂÃÂÃÂÃÂÃÂÃÂÃÂ 53
                     62ÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂ /ÃÂÃÂÃÂÃÂÃÂÃÂ
                     ÃÂÃÂ #ccffccÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂ 
                     /ÃÂÃÂ
                     ÃÂÃÂÃÂÃÂÃÂÃÂ signalÃÂÃÂÃÂÃÂÃÂÃÂÃÂ
                     ÃÂ sequence="This feature has 2 segments:
                     ÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂ ÃÂÃÂÃÂÃÂÃÂÃÂÃ
                     ÂÃÂ ÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂ 2:ÃÂÃÂÃÂÃÂ
                     ÃÂÃÂÃÂÃÂ 5363ÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂ ..ÃÂ
                     ÃÂÃÂÃÂÃÂÃÂÃÂÃÂ 61
                     54ÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂ /ÃÂÃÂÃÂÃÂÃÂÃÂ
                     ÃÂÃÂ #ccffcc Cleavage site after base 5362"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     I
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     S
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     W
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     A
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     S LIKHW"
                     /locus_tag="beta-lactamase"
                     /label="beta-lactamase"
                     /ApEinfo_label="beta-lactamase"
                     /ApEinfo_fwdcolor="#ff8080"
                     /ApEinfo_revcolor="#ffff80"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_signal     2931..2937
                     /gene="GAL1 TATA"
                     /SECDrawAs="Label"
                     /locus_tag="GAL1 TATA"
                     /label="GAL1 TATA"
                     /ApEinfo_label="GAL1 TATA"
                     /ApEinfo_fwdcolor="#00ff2c"
                     /ApEinfo_revcolor="#00ff2c"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_signal     3089..3208
                     /gene="3xFlag-NLS"
                     /SECDrawAs="Label"
                     /locus_tag="3xFlag-NLS"
                     /label="3xFlag-NLS"
                     /ApEinfo_label="3xFlag-NLS"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     rep_origin      8312..8900
                     /direction=RIGHT
                     /note="ori"
                     /note="high-copy-number colE1/pMB1/pBR322/pUC origin of
                     replication"
                     /note="color: #ffff00"
                     /locus_tag="ori"
                     /label="ori"
                     /ApEinfo_label="ori"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     promoter        9224..9254
                     /note="lac promoter"
                     /note="promoter for the E. coli lac operon"
                     /note="This forward directional feature has 3 segments:
                     ÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂ ÃÂÃÂÃÂÃÂÃÂÃÂÃ
                     ÂÃÂ ÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂ 1:ÃÂÃÂÃÂÃÂ
                     ÃÂÃÂÃÂÃÂ 7237ÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂ ..ÃÂ
                     ÃÂÃÂÃÂÃÂÃÂÃÂÃÂ 72
                     42ÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂ /ÃÂÃÂÃÂÃÂÃÂÃÂ
                     ÃÂÃÂ #ffffffÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂ 
                     /ÃÂÃÂ ÃÂÃÂÃÂÃÂÃÂÃÂ -35="This forward
                     directional feature has 3 segments:
                     ÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂ ÃÂÃÂÃÂÃÂÃÂÃÂÃ
                     ÂÃÂ ÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂ 2:ÃÂÃÂÃÂÃÂ
                     ÃÂÃÂÃÂÃÂ 7243ÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂ ..ÃÂ
                     ÃÂÃÂÃÂÃÂÃÂÃÂÃÂ 72
                     60ÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂ /ÃÂÃÂÃÂÃÂÃÂÃÂ
                     ÃÂÃÂ #ffffff
                     ÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂ ÃÂÃÂÃÂÃÂÃÂÃÂÃ
                     ÂÃÂ ÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂ 3:ÃÂÃÂÃÂÃÂ
                     ÃÂÃÂÃÂÃÂ 7261ÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂ ..ÃÂ
                     ÃÂÃÂÃÂÃÂÃÂÃÂÃÂ 72
                     67ÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂ /ÃÂÃÂÃÂÃÂÃÂÃÂ
                     ÃÂÃÂ #ffffffÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂ 
                     /ÃÂÃÂ ÃÂÃÂÃÂÃÂÃÂÃÂ -10"="This forward
                     directional feature has 3 segments:
                     /locus_tag="lac promoter"
                     /label="lac promoter"
                     /ApEinfo_label="lac promoter"
                     /ApEinfo_fwdcolor="#00ffff"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     protein_bind    9262..9278
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /note="color: #31849b"
                     /locus_tag="lac operator"
                     /label="lac operator"
                     /ApEinfo_label="lac operator"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_signal     2952..2986
                     /gene="OLac"
                     /SECDrawAs="Label"
                     /locus_tag="OLac"
                     /label="OLac"
                     /ApEinfo_label="OLac"
                     /ApEinfo_fwdcolor="#ff0015"
                     /ApEinfo_revcolor="#ff0015"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             3089..3208
                     /gene="3xFlag-NLS"
                     /SECDrawAs="Gene"
                     /locus_tag="3xFlag-NLS(1)"
                     /label="3xFlag-NLS(1)"
                     /ApEinfo_label="3xFlag-NLS"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     9286..9302
                     /note="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /note="color: #a020f0; direction: RIGHT"
                     /locus_tag="M13 rev"
                     /label="M13 rev"
                     /ApEinfo_label="M13 rev"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     promoter        9323..9341
                     /note="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
                     /note="color: #ffffff; direction: RIGHT"
                     /locus_tag="T3 promoter"
                     /label="T3 promoter"
                     /ApEinfo_label="T3 promoter"
                     /ApEinfo_fwdcolor="#00ffff"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    7479..8138
                     /locus_tag="AmpR"
                     /label="AmpR"
                     /ApEinfo_label="AmpR"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             3536..4606
                     /translation="MDKAELIPEPPKKKRKVELGTAANFNQSGNIADSSLSFTFTNSS
                     NGPNLITTQTNSQALSQPIASSNVHDNFMNNEITASKIDDGNNSKPLSPGWTDQTAYN
                     AFGITTGMFNTTTMDDVYNYLFDDEDTPPNPKKEIFNTTHYRASAEASATMNGAIGGD
                     LLLNFPDMSVLERQRAHLKYLNPTFDSPLAGFFADSSMITGGEMDSYLSTAGLNLPMM
                     YGETTVEGDSRLSISPETTLGTGNFKKRKFDTETKDCNEKKKKMTMNRDDLVEEGEEE
                     KSKITEQNNGSTKSIKKMKHKAKKEENNFSNDSSKVTKELEKTDYIHVRARRGQATDS
                     HSIAERVRREKISERMKFLQDLVPGCDKITGKAGMLDEIINYVQSLQRQIEFLSMKLA
                     IVNPRPDFDMDDIFAKEVASTPMTVVPSPEMVLSGYSHEMVHSGYSSEMVNSGYLHVN
                     PMQQVNTSSDPLSCFNNGEAPSMWDSHVQNLYGNLGVPVIELELQMNRRY*"
                     /locus_tag="ORF frame 1"
                     /label="ORF frame 1"
                     /ApEinfo_label="ORF frame 1"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     source          3536..4606
                     /organism="pGal4AD-CIB1"
                     /mol_type="other DNA"
                     /locus_tag="CIB1"
                     /label="CIB1"
                     /ApEinfo_label="CIB1"
                     /ApEinfo_fwdcolor="#72f088"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
ORIGIN
        1 ggccggccat ttaaatgttt aaacggccac gctggccgag gagaacttct agtatatcta
       61 catacctaat attattgcct tattaaaaat ggaatcccaa caattacatc aaaatccaca
      121 ttctcttcaa aatcaattgt cctgtacttc cttgttcatg tgtgttcaaa aacgttatat
      181 ttataggata attatactct atttctcaac aagtaattgg ttgtttggcc gagcggtcta
      241 aggcgcctga ttcaagaaat atcttgaccg cagttaactg tgggaatact caggtatcgt
      301 aagatgcaag agttcgaatc tcttagcaac cattattttt ttcctcaaca taacgagaac
      361 acacaggggc gctatcgcac agaatcaaat tcgatgactg gaaatttttt gttaatttca
      421 gaggtcgcct gacgcatata cctttttcaa ctgaaaaatt gggagaaaaa ggaaaggtga
      481 gagcgccgga accggctttt catatagaat agagaagcgt tcatgactaa atgcttgcat
      541 cacaatactt gaagttgaca atattattta aggacctatt gttttttcca ataggtggtt
      601 agcaatcgtc ttactttcta acttttctta ccttttacat ttcagcaata tatatatata
      661 tatttcaagg atataccatt ctaatgtctg cccctaagtt accaatagat agcggtttaa
      721 tataaggttt caacaagaaa tattttgtga aaatcaaaag ttaatagttt cactgtcgaa
      781 aatatgaaag tttaagtttt cagttcatcc cattgtagtt atcaaatttc tgtgtttccc
      841 gtatatgtgt gctctgtatt catcgctatc ttgaaactgg ttatgatcgt tgcgcatgtg
      901 acctgtatcg ctcaaaagag cccggcaccg gtaccgggca aaagccccta gcaagctaaa
      961 atgattcaac tttcgtagta atttgttcaa ccggtcacag taaagtcaat gactaacttt
     1021 tgaatcgagg atgtatttcc aattgaaagc aatgtatata taaaccattg tgttgaagct
     1081 tgtcttacag ttttcttgta tcctcccatt gttcatatag tacttaaagt accataatcc
     1141 ttaaagaaca aacttgaaag agaatacact atacacaact aataaataaa gaataatcat
     1201 ggctgtgacc aagacaattg tagttctacc aggtgaccat gttggtcaag aaatcactga
     1261 agaggccatt aaagttttaa atgctattca ggaatgtcgt ccagacaagg tcaatttcaa
     1321 gtttgatcat catttgatcg gtggtgctgc aattgatgcc actggtgttc cattaccaga
     1381 cgaagctttg gaggcctcta agaaagctga tgccgtgctt ctgggtgctg ttggtggtcc
     1441 aaaatggggt actggcgctg tcagaccaga acaaggtctt ttgaaaatcc gtaaggagtt
     1501 gcaattgtat gctaatctaa gaccatgtaa ttttgcatct gattccttac tagatctatc
     1561 gccattgaag cctgaaattg caagaggtac agatttcgtc gttgttagag aactagtggg
     1621 tggtatttat ttcggggaga gaaaagaaga tgaaggtgat ggtgtcgcct gggatagcga
     1681 aaagtattct gtgcccgaag ttcaaagaat cactagaatg gctgctttca tggctctgca
     1741 acacaaccca cctttaccta tttggtctct agataaagcc aacgttctag catcatcaag
     1801 attatggaga aaaacagtgg aagagacgat taagaatgaa ttcccacaat tgactgttca
     1861 acatcaattg attgattccg ctgctatgat cttggttaag aacccaactc atctaaatgg
     1921 tattattatc acaaacaata tgtttggtga tattatctcc gatgaagcct ccgtgatccc
     1981 aggttcgtta ggattacttc catctgcctc cttggcatct cttccagata agaatacggc
     2041 atttggttta tatgagccat gtcatggctc tgctccagac ttaccaaagg gaaaggttaa
     2101 ccccgtagca acaatcctat ctgctgctat gatgttgaag ttgtctctgg acctgttcga
     2161 agaaggtgaa attatagaac aagcagtcaa gaaagtgttg gattctggta tcagaacagc
     2221 ggatctaaag ggtaccaact ctactactga ggtcggtgat gcagtagcga aagctgtcag
     2281 ggaactatta gcttagactt agtagatgta cagttgcata cataagtctg aacagaaaga
     2341 agtaaatatc atatctagtt cggtttagaa atcagtgact atattggttt tgaccagatc
     2401 gcaatccttg caagcattta gtaatgacta tcaaaccggt gaatcatctg ataaggctat
     2461 attagcttgt gcattcgcat gtatcgggaa acgaacggta ccgggccagc agatccgcca
     2521 ggcgtgtata tatagcgtgg atggccaggc aactttagtg ctgacacata caggcatata
     2581 tatatgtgtg cgacgacaca tgatcatatg GGTACCgaag tacggattag aagccgccga
     2641 gcgggtgaca gccctccgaa ggaagactct cctccgtgcg tcctcgtctt caccggtcgc
     2701 gttcctgaaa cgcagatgtg cctcgcgccg cactgctccg aacaataaag attctacaat
     2761 actagctttt atggttatga agaggaaaaa ttggcagtaa cctggcccca caaaccttca
     2821 aatgaacgaa tcaaattaac aaccatagga tgataatgcg attacttttt tagccttatt
     2881 tctggggtac tgcagcagcg aagcgatgat ttttgatcta ttaacagata tataaatgca
     2941 aaaactgttg ttgtgtggaa ttgtgagcgg ataacaattt cacacaatat tacttcttat
     3001 tcaaatgtaa taaaagtatc aacaaaaaat tgttaatata cctctatact ttaacgtcaa
     3061 ggagaaaaac cccttGGGCC Ctaaaaaaat ggactacaaa gaccatgacg gtgattataa
     3121 agatcatgac atcgattaca aggatgacga tgacaagatg gcccccaaga aaaagaggaa
     3181 ggtgggcatt cacggggtgc cgggtggact cgagggttct ggttCCTGCA GGgctccacc
     3241 aactgatgtt tcattgggtg atgaattgca tttggatgga gaagatgttg ctatggctca
     3301 tgctgatgca ttagatgatt tcgatttaga catgttggga gatggtgatt ctccaggtcc
     3361 aggtttcact ccacatgatt ctgctcctta cggtgcattg gatatggctg attttgaatt
     3421 cgaacaaatg ttcactgatg ctttaggaat tgatgaatat ggtggtggtG CGGCCGCtgg
     3481 aggttctggc ggtggaagtg gtggcggagg tagcggtggt ggcagcggtc ccgggGCGAG
     3541 CGCCGAAGCT AGCGCCACCa tgaatggagc tataggaggt gaccttttgc tcaattttcc
     3601 tgacatgtcg gtcctagagc gccaaagggc tcacctcaag tacctcaatc ccacctttga
     3661 ttctcctctc gccggcttct ttgccgattc ttcaatgatt accggcggcg agatggacag
     3721 ctatctttcg actgccggtt tgaatcttcc gatgatgtac ggtgagacga cggtggaagg
     3781 tgattcaaga ctctcaattt cgccggaaac gacgcttggg actggaaatt tcaagaaacg
     3841 gaagtttgat acagagacta aggattgtaa tgagaagaag aagaagatga cgatgaacag
     3901 agatgaccta gtagaagaag gagaagaaga gaagtcgaaa ataacagagc aaaacaatgg
     3961 gagcacaaaa agcatcaaga agatgaaaca caaagccaag aaagaagaga acaatttctc
     4021 taatgattca tctaaagtga cgaaggaatt ggagaaaacg gattatattc atgttcgtgc
     4081 acgacgaggc caagccactg atagtcacag catagcagaa cgagttagaa gagaaaagat
     4141 cagtgagaga atgaagtttc tacaagattt ggttcctgga tgcgacaaga tcacaggcaa
     4201 agcagggatg cttgatgaaa tcattaacta tgttcagtct cttcagagac aaatcgagtt
     4261 cttatcgatg aaactagcaa ttgtgaatcc aaggccggat tttgatatgg atgacatttt
     4321 tgccaaagag gttgcctcaa ctccaatgac tgtggtgcca tctcctgaaa tggttctttc
     4381 cggttattct catgagatgg ttcactctgg ttattctagt gagatggtta actccggtta
     4441 ccttcatgtc aatccaatgc agcaagtgaa taccagttct gatccattgt catgcttcaa
     4501 caatggcgaa gctccttcga tgtgggactc tcatgtgcag aatctctatg gcaatttagg
     4561 agtACCGGTC ATCGAGCTCG AGCTGCAGAT GAATCGTAGA TACTGAggat cctaaGCGCC
     4621 ACCGCGGTGg agctctaagc aaatagctaa attatatacg aattaatatt atgattaagt
     4681 gtttacgtga gtgcgatatt tttattacta tcttatacag ttgtatatac tctataaaat
     4741 gagttgtcta ttaattaacg cgatgaatgc tttctgggtt tacctctcca acaactctag
     4801 tttacttctc aatacattca attgtatttg atttgtcaat acttcatcat taatcaattc
     4861 tatagttttg tttttctcgt ttatttccaa atttaatgca tcaattttat tattcaattt
     4921 gtcgttgatt ttggttaatg attttatggt ttgatctctg gcattgattg tttgtgttag
     4981 tttttcatta ttgataatta aattatttaa gttagttatc aactcggtgt tttcaagttt
     5041 caagttttca atttctttag agtttattag atttgtcaaa gtttctgaat tgcttgattg
     5101 gtcctgtaga agagtatttg ttgttgtgga taattgattc aatttttgag acaattgctg
     5161 gaaggcgttg aaatatctag catcaatctc atggtttttt tcccgagagt ctcgtagatt
     5221 caattgtttt aatatatctt gggaccactc ttgatttgaa ctcatggaaa ttaaactggg
     5281 tgttgtgttg tggtgtaatg attgtacccc ctttgcttat aattgtgtgg ccaccgaagt
     5341 cggtgatgct gtcgccgaag aagttaagaa aatccttgct taaaaagatt ctcttttttt
     5401 atgatatttg tacataaact ttataaatga aattcataat agaaacgaca cgaaattaca
     5461 aaatggaata tgttcatagg gtagacgaaa ctatatacgc aatctacata catttatcaa
     5521 gaaggagaaa aaggaggatg taaaggaata caggtaagca aattgatact aatggctcaa
     5581 cgtgataagg aaaaagaatt gcactttaac attaatattg acaaggagga gggcaccaca
     5641 caaaaagtta ggtgtaacag aaaatcatga aactatgatt cctaatttat atattggagg
     5701 attttctcta aaaaaaaaaa aatacaacaa ataaaaaaca ctcaatgacc tgaccatttg
     5761 atggagttta agtcaatacc ttcttgaacc atttcccata atggtgaaag ttccctcaag
     5821 aattttactc tgtcagaaac ggccttaacg acgtaggccg gccatttaaa tgtttaaacg
     5881 gccacgctgg cccaattcgc cctatagtga gtcgtattac aattcactgg ccgtcgtttt
     5941 acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc
     6001 ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt
     6061 gcgcagcctg aatggcgaat ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg
     6121 gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct
     6181 ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg
     6241 ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag
     6301 ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg
     6361 gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc
     6421 tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat
     6481 gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgct tacaatttcc
     6541 tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata gggtaataac
     6601 tgatataatt aaattgaagc tctaatttgt gagtttagta ttaggatctc tacccttggc
     6661 gaaaagtcct ctgccaacaa tgatgatatc tgatccacca cttacaactt cgtcgacggt
     6721 tctgtactgc tgacccaata tgcgtatata taccaatcta agtctgtgct ccttccttcg
     6781 ttcttccttc tgttcggaga ttaccgaatc aaaaaaattt caaggaaacc gaaatcaaaa
     6841 aaaagaataa aaaaaaaatg atgaattgaa aaggtggtat ggtgcactct cagtacaatc
     6901 tgctctgatg ccgcatagtt aagccagccc cgacacccgc caacacccgc tgacgcgccc
     6961 tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc
     7021 tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg
     7081 atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcggcgcgc
     7141 cgaggacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
     7201 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
     7261 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
     7321 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
     7381 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
     7441 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
     7501 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
     7561 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
     7621 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
     7681 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
     7741 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
     7801 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
     7861 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
     7921 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
     7981 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
     8041 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     8101 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     8161 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     8221 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     8281 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     8341 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     8401 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     8461 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     8521 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     8581 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     8641 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     8701 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     8761 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     8821 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     8881 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     8941 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     9001 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     9061 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     9121 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
     9181 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc
     9241 cggctcctat gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg
     9301 accatgatta cgccaagctc ggaattaacc ctcactaaag ggaacaaaag ctg
//
