LOCUS       Exported                9269 bp ds-DNA     circular SYN 16-JUL-2021
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9269)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Jul 16, 2021 from SnapGene Viewer 5.1.4.1
            https://www.snapgene.com
COMMENT     Created by Clontech Laboratories Inc. http://www.clontech.com  Cat. 
            No. 632158,632162,632157,632163
FEATURES             Location/Qualifiers
     source          1..9269
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     /label=source:synthetic DNA construct
                     /note="/locus_tag=source:synthetic DNA construct"
                     /note="/locus_tag=source:synthetic DNA construct"
                     /note="/locus_tag=source:synthetic DNA construct"
                     /note="/locus_tag=source:synthetic DNA construct"
                     /note="/locus_tag=source:synthetic DNA construct"
                     /note="color: #ffc0cb"
     LTR             1..635
                     /locus_tag="5' LTR"
                     /label=5' LTR
                     /note="color: #86f71d"
     misc_binding    636..653
                     /locus_tag="PBS"
                     /label=PBS
                     /note="color: #ff4809"
     misc_feature    685..822
                     /locus_tag="Packaging signal"
                     /label=Packaging signal
                     /note="color: #7eff74"
     misc_binding    1303..1536
                     /locus_tag="RRE"
                     /label=RRE
                     /note="color: #ff4809"
     misc_feature    2028..2151
                     /locus_tag="cPPT/CTS"
                     /label=cPPT/CTS
                     /note="color: #7eff74"
     promoter        2205..2519
                     /locus_tag="TREtight Promoter"
                     /label=TREtight Promoter
                     /note="color: #346ee0"
     misc_feature    2527..2532
                     /locus_tag="BAMHI"
                     /label=BAMHI
                     /note="color: #7eff74"
     misc_feature    2533..2541
                     /locus_tag="KOZAK"
                     /label=KOZAK
                     /note="color: #7eff74"
     misc_feature    2542..2571
                     /locus_tag="Myc TAG"
                     /label=Myc TAG
                     /note="color: #ffcc00"
     misc_feature    2587..4038
                     /locus_tag="CLK1"
                     /label=CLK1
                     /note="color: #a6acb3"
     misc_feature    2587..2589
                     /locus_tag="CLK Start"
                     /label=CLK Start
                     /note="color: #7eff74"
     primer_bind     complement(4018..4069)
                     /locus_tag="CLK1 Gibson Reverse"
                     /label=CLK1 Gibson Reverse
                     /note="color: #a020f0; direction: LEFT"
     misc_feature    4039..4044
                     /locus_tag="STOP"
                     /label=STOP
                     /note="color: #a6acb3"
     misc_feature    4045..4050
                     /locus_tag="EcoRI"
                     /label=EcoRI
                     /note="color: #7eff74"
     promoter        4051..4554
                     /locus_tag="PGK Promoter"
                     /label=PGK Promoter
                     /note="color: #346ee0"
     CDS             4575..5174
                     /locus_tag="Puro(R)"
                     /label=Puro(R)
                     /note="color: #e9d024; direction: RIGHT"
     misc_feature    5191..5782
                     /locus_tag="WPRE"
                     /label=WPRE
                     /note="color: #7eff74"
     LTR             5985..6621
                     /locus_tag="3' LTR"
                     /label=3' LTR
                     /note="color: #86f71d"
     rep_origin      complement(7090..7763)
                     /direction=LEFT
                     /locus_tag="pUC origin"
                     /label=pUC origin
                     /note="color: #999999"
     CDS             complement(7908..8904)
                     /locus_tag="Amp(R)"
                     /label=Amp(R)
                     /note="color: #e9d024; direction: LEFT"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgatgaggcc ctttcgtctt cactcgagtt tactccctat
     2221 cagtgataga gaacgtatgt cgagtttact ccctatcagt gatagagaac gatgtcgagt
     2281 ttactcccta tcagtgatag agaacgtatg tcgagtttac tccctatcag tgatagagaa
     2341 cgtatgtcga gtttactccc tatcagtgat agagaacgta tgtcgagttt atccctatca
     2401 gtgatagaga acgtatgtcg agtttactcc ctatcagtga tagagaacgt atgtcgaggt
     2461 aggcgtgtac ggtgggaggc ctatataagc agagctcgtt tagtgaccgt cagatcgcct
     2521 ggagaaggat ccgccaccat ggagcagaag ctgatcagcg aggaggacct gggaggtggc
     2581 ggtagcatga gacactcaaa gagaacttac tgtcctgatt gggatgacaa ggattgggat
     2641 tatggaaaat ggaggagcag cagcagtcat aaaagaagga agagatcaca tagcagtgcc
     2701 caggagaaca agcgctgcaa atacaatcac tctaaaatgt gtgatagcca ttatttggaa
     2761 agcaggtcta taaatgagaa agattatcat agtcgacgct acattgatga gtacagaaat
     2821 gactacactc aaggatgtga acctggacat cgccaaagag accatgaaag ccggtatcag
     2881 aaccatagta gcaagtcttc tggtagaagt ggaagaagta gttataaaag caaacacagg
     2941 attcaccaca gtacttcaca tcgtcgttca catgggaaga gtcaccgaag gaaaagaacc
     3001 aggagtgtag aggatgatga ggagggtcac ctgatctgtc agagtggaga cgtactaagt
     3061 gcaagatatg aaattgttga tactttaggt gaaggagctt ttggaaaagt tgtggagtgc
     3121 atcgatcata aagcgggagg tagacatgta gcagtaaaaa tagttaaaaa tgtggataga
     3181 tactgtgaag ctgctcgctc agaaatacaa gttctggaac atctgaatac aacagacccc
     3241 aacagtactt tccgctgtgt ccagatgttg gaatggtttg agcatcatgg tcacatttgc
     3301 attgtttttg aactattggg acttagtact tacgacttca ttaaagaaaa tggttttcta
     3361 ccatttcgac tggatcatat cagaaagatg gcatatcaga tatgcaagtc tgtgaatttt
     3421 ttgcacagta ataagttgac tcacacagac ttaaagcctg aaaacatctt atttgtgcag
     3481 tctgactaca cagaggcgta taatcccaaa ataaaacgtg atgaacgcac cttaataaat
     3541 ccagatatta aagttgtaga ctttggtagt gcaacatatg atgacgaaca tcacagtaca
     3601 ttggtatcta caagacatta tagagcacct gaagttattt tagccctagg gtggtcccaa
     3661 ccatgtgatg tctggagcat aggatgcatt cttattgaat actatcttgg gtttaccgta
     3721 tttccaacac acgatagtaa ggagcattta gcaatgatgg aaaggattct tggacctcta
     3781 ccaaaacata tgatacagaa aaccaggaaa cgtaaatatt ttcaccacga tcgattagac
     3841 tgggatgaac acagttctgc cggcagatat gtttcaagac gctgtaaacc tctgaaggaa
     3901 tttatgcttt ctcaagatgt tgaacatgag cgtctctttg acctcattca gaaaatgttg
     3961 gagtatgatc cagccaaaag aattactctc agagaagcct taaagcatcc tttctttgac
     4021 cttctgaaga aaagtatata gtaagaattc taccgggtag gggaggcgct tttcccaagg
     4081 cagtctggag catgcgcttt agcagccccg ctgggcactt ggcgctacac aagtggcctc
     4141 tggcctcgca cacattccac atccaccggt aggcgccaac cggctccgtt ctttggtggc
     4201 cccttcgcgc caccttctac tcctccccta gtcaggaagt tcccccccgc cccgcagctc
     4261 gcgtcgtgca ggacgtgaca aatggaagta gcacgtctca ctagtctcgt gcagatggac
     4321 agcaccgctg agcaatggaa gcgggtaggc ctttggggca gcggccaata gcagctttgc
     4381 tccttcgctt tctgggctca gaggctggga aggggtgggt ccgggggcgg gctcaggggc
     4441 gggctcaggg gcggggcggg cgcccgaagg tcctccggag gcccggcatt ctgcacgctt
     4501 caaaagcgca cgtctgccgc gctgttctcc tcttcctcat ctccgggcct ttcgacctgc
     4561 agcccaagct taccatgacc gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg
     4621 tccccagggc cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca
     4681 ccgtcgatcc ggaccgccac atcgagcggg tcaccgagct gcaagaactc ttcctcacgc
     4741 gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct
     4801 ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg
     4861 ccgagttgag cggttcccgg ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc
     4921 accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg
     4981 gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg
     5041 tgcccgcctt cctggagacc tccgcgcccc gcaacctccc cttctacgag cggctcggct
     5101 tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc atgacccgca
     5161 agcccggtgc ctgacgggcg cgtctggaac aatcaacctc tggattacaa aatttgtgaa
     5221 agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta
     5281 atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa
     5341 tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg
     5401 tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc
     5461 ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc
     5521 cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg
     5581 gggaagctga cgtcctttcc atggctgctc gcctgtgttg ccacctggat tctgcgcggg
     5641 acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg
     5701 ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc
     5761 ctttgggccg cctccccgcc tggaattaat tctgcagtcg agacctagaa aaacatggag
     5821 caatcacaag tagcaataca gcagctacca atgctgattg tgcctggcta gaagcacaag
     5881 aggaggagga ggtgggtttt ccagtcacac ctcaggtacc tttaagacca atgacttaca
     5941 aggcagctgt agatcttagc cactttttaa aagaaaagag gggactggaa gggctaattc
     6001 actcccaacg aagacaagat atccttgatc tgtggatcta ccacacacaa ggctacttcc
     6061 ctgattagca gaactacaca ccagggccag gggtcagata tccactgacc tttggatggt
     6121 gctacaagct agtaccagtt gagccagata aggtagaaga ggccaataaa ggagagaaca
     6181 ccagcttgtt acaccctgtg agcctgcatg ggatggatga cccggagaga gaagtgttag
     6241 agtggaggtt tgacagccgc ctagcatttc atcacgtggc ccgagagctg catccggagt
     6301 acttcaagaa ctgctgatat cgagcttgct acaagggact ttccgctggg gactttccag
     6361 ggaggcgtgg cctgggcggg actggggagt ggcgagccct cagatcctgc atataagcag
     6421 ctgctttttg cctgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct
     6481 ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta
     6541 gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca
     6601 gtgtggaaaa tctctagcag tagtagttca tgtcatctta ttattcagta tttataactt
     6661 gcaaagaaat gaatatcaga gagtgagagg ccttgacatt gctagcgttt accgtcgacc
     6721 tctagctaga gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg
     6781 ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa
     6841 tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac
     6901 ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt
     6961 gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
     7021 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
     7081 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
     7141 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
     7201 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
     7261 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
     7321 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
     7381 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
     7441 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
     7501 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
     7561 tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag
     7621 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
     7681 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
     7741 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
     7801 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
     7861 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
     7921 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
     7981 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
     8041 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga
     8101 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
     8161 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
     8221 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
     8281 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
     8341 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
     8401 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
     8461 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
     8521 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
     8581 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
     8641 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
     8701 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
     8761 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
     8821 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
     8881 ccccgaaaag tgccacctga cgtcgacgga tcgggagatc aacttgttta ttgcagctta
     8941 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
     9001 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggatcaactg
     9061 gataactcaa gctaaccaaa atcatcccaa acttcccacc ccatacccta ttaccactgc
     9121 caattacctg tggtttcatt tactctaaac ctgtgattcc tctgaattat tttcatttta
     9181 aagaaattgt atttgttaaa tatgtactac aaacttagta gtttttaaag aaattgtatt
     9241 tgttaaatat gtactacaaa cttagtagt
//
