LOCUS       VB190809-1318nje        9410 bp    DNA     circular SYN 12-MAR-2021
DEFINITION  
ACCESSION   VB190809-1318nje
VERSION     VB190809-1318nje
KEYWORDS    .
SOURCE      
  ORGANISM  .
            
FEATURES             Location/Qualifiers
     source          1..9410
     Promoter        1..229
                     /application_notes="Strong promoter; drives transcription
                     of viral RNA in packaging cells."
                     /description="Rous sarcoma virus enhancer/promoter"
                     /full_name="Rous sarcoma virus (RSV) enhancer/promoter"
                     /label="RSV  promoter"
                     /note="color: #5566f5; direction: RIGHT"
                     /official_designation="RSV"
                     /uuid="3f2ddca7f0cb4015b6abcec8a25c834e"
     LTR             230..410
                     /application_notes="Allows transcription of viral RNA and
                     its packaging into virus."
                     /description="Truncated HIV-1 5' long terminal repeat"
                     /full_name="HIV-1 truncated 5' LTR"
                     /label="Δ5' LTR"
                     /note="color: #ef6cdf; direction: RIGHT"
                     /official_designation="Delta5' LTR"
                     /uuid="44156a3083604321be44066133e8ecb9"
     Miscellaneous   521..565
                     /application_notes="Allows packaging of viral DNA into
                     virus."
                     /description="Adenovirus packaging signal"
                     /full_name="HIV-1 psi packaging signal"
                     /label="Ψ"
                     /note="color: #c54b7c; direction: RIGHT"
                     /official_designation="Psi"
                     /uuid="284899413ff943a99f8210a11ad8c54e"
     Miscellaneous   1075..1308
                     /application_notes="Rev protein binding site that allows
                     Rev-dependent nuclear export of viral RNA during viral
                     packaging."
                     /description="HIV-1 Rev response element"
                     /full_name="HIV-1 Rev response element"
                     /label="RRE"
                     /note="color: #d05c0a; direction: RIGHT"
                     /official_designation="RRE"
                     /uuid="df07099e49674cf3aba3661b54e28bd7"
     Miscellaneous   1803..1920
                     /application_notes="Facilitates the nuclear import of HIV-1
                     cDNA through a central DNA flap."
                     /description="Central polypurine tract"
                     /full_name="Central polypurine tract"
                     /label="cPPT"
                     /note="color: #fd3434; direction: RIGHT"
                     /official_designation="cPPT"
                     /uuid="f7cb29985f9f41a7a5924d3462266f42"
     Promoter        1959..3137
                     /application_notes="Strong promoter."
                     /description="Human eukaryotic translation elongation
                     factor 1 alpha1 promoter"
                     /full_name="Human eukaryotic translation elongation factor
                     1 alpha1 promoter"
                     /label="EF1A"
                     /note="color: #46c6ef; direction: RIGHT"
                     /official_designation="EF1A"
                     /uuid="69c052aac71a4f49bab5b7ae1f46ace8"
     Miscellaneous   3162..3167
                     /application_notes="Facilitates translation initiation of
                     ATG start codon downstream of the Kozak sequence."
                     /description="Kozak translation initiation sequence"
                     /full_name="Kozak"
                     /label="Kozak"
                     /note="color: #eda481; direction: RIGHT"
                     /official_designation="Kozak"
                     /uuid="5f18b25a1234443494a618363f09f052"
     CDS             3168..4082
                     /application_notes=""
                     /description="G&gt;A substitution at base 856 to create
                     T286A mutation"
                     /label="hCCND1[NM_053056.2]*/HA"
                     /note="color: #68e66d; direction: RIGHT"
                     /official_designation="hCCND1[NM_053056.2]"
                     /uuid="f16cb593d6c149c39672ea6917f75e15"
     Miscellaneous   4121..4718
                     /application_notes="Enhances virus stability in packaging
                     cells, leading to higher titer of packaged virus; enhances
                     higher expression of transgenes."
                     /description="Woodchuck hepatitis virus posttranscriptional
                     regulatory element"
                     /full_name="Woodchuck hepatitis virus posttranscriptional
                     regulatory element"
                     /label="WPRE"
                     /note="color: #da3bb5; direction: RIGHT"
                     /official_designation="WPRE"
                     /uuid="0aedff8308474b369c622433538d7836"
     Promoter        4737..5247
                     /application_notes="Medium-strength promoter."
                     /description="Mouse phosphoglycerate kinase 1 promoter"
                     /full_name="Mouse phosphoglycerate kinase 1 promoter"
                     /label="mPGK promoter"
                     /note="color: #e4b930; direction: RIGHT"
                     /official_designation="mPGK promoter"
                     /uuid="bce93795b5fd4173b8b9abc832edad80"
     Marker          5260..5859
                     /application_notes="Allows cells to be resistant to
                     puromycin."
                     /description="Puromycin resistance gene"
                     /full_name="Puromycin resistance gene"
                     /label="Puro"
                     /note="color: #7a00b5; direction: RIGHT"
                     /official_designation="Puro"
                     /uuid="39d7413208494d9291d10b21b13ca44c"
     LTR             5930..6164
                     /application_notes="Allows packaging of viral RNA into
                     virus; self-inactivates the 5' LTR by a copying mechanism
                     during viral genome integration; contains polyadenylation
                     signal for transcription termination."
                     /description="Truncated HIV-1 3' long terminal repeat"
                     /full_name="HIV-1 truncated 3' LTR"
                     /label="3' LTR-ΔU3"
                     /note="color: #05696d; direction: RIGHT"
                     /official_designation="DeltaU3/3' LTR"
                     /uuid="c958755414c34372b909e20e81ebcef6"
     PolyA_signal    6237..6371
                     /application_notes="Allows transcription termination and
                     polyadenylation of mRNA transcribed by Pol II RNA
                     polymerase."
                     /description="Simian virus 40 early polyadenylation signal"
                     /full_name="SV40 early polyadenation signal"
                     /label="SV40 early pA"
                     /note="color: #f54c6b; direction: RIGHT"
                     /official_designation="SV40 early pA"
                     /uuid="2fcb13a62e704d8095b188d9625a6a94"
     ORF             7325..8185
                     /application_notes="Allows E. coli to be resistant to
                     ampicillin."
                     /description="Ampicillin resistance gene"
                     /full_name="Ampicillin resistance gene"
                     /label="Ampicillin"
                     /note="color: #944603; direction: RIGHT"
                     /official_designation="Ampicillin"
                     /uuid="6a7994dc58024d80b36e4a85ad2e1d99"
     Rep_origin      8356..8944
                     /application_notes="Facilitates plasmid replication in E.
                     coli; regulates high-copy plasmid number (500-700)."
                     /description="pUC origin of replication"
                     /full_name="pUC origin of replication"
                     /label="pUC ori"
                     /note="color: #ce0084; direction: RIGHT"
                     /official_designation="pUC ori"
                     /uuid="64a8cb3def9f49deb6752795c2d893a8"
ORIGIN
        1 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca
       61 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga
      121 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt
      181 gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg
      241 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
      301 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
      361 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
      421 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
      481 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
      541 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
      601 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
      661 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
      721 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
      781 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
      841 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
      901 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga
      961 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     1021 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     1081 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     1141 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     1201 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     1261 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     1321 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     1381 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcgcta
     1801 gcttttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata
     1861 atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt
     1921 actagtgatt atcggatcaa ctttgtatag aaaagttggg ctccggtgcc cgtcagtggg
     1981 cagagcgcac atcgcccaca gtccccgaga agttgggggg aggggtcggc aattgaaccg
     2041 gtgcctagag aaggtggcgc ggggtaaact gggaaagtga tgtcgtgtac tggctccgcc
     2101 tttttcccga gggtggggga gaaccgtata taagtgcagt agtcgccgtg aacgttcttt
     2161 ttcgcaacgg gtttgccgcc agaacacagg taagtgccgt gtgtggttcc cgcgggcctg
     2221 gcctctttac gggttatggc ccttgcgtgc cttgaattac ttccacctgg ctgcagtacg
     2281 tgattcttga tcccgagctt cgggttggaa gtgggtggga gagttcgagg ccttgcgctt
     2341 aaggagcccc ttcgcctcgt gcttgagttg aggcctggcc tgggcgctgg ggccgccgcg
     2401 tgcgaatctg gtggcacctt cgcgcctgtc tcgctgcttt cgataagtct ctagccattt
     2461 aaaatttttg atgacctgct gcgacgcttt ttttctggca agatagtctt gtaaatgcgg
     2521 gccaagatct gcacactggt atttcggttt ttggggccgc gggcggcgac ggggcccgtg
     2581 cgtcccagcg cacatgttcg gcgaggcggg gcctgcgagc gcggccaccg agaatcggac
     2641 gggggtagtc tcaagctggc cggcctgctc tggtgcctgg tctcgcgccg ccgtgtatcg
     2701 ccccgccctg ggcggcaagg ctggcccggt cggcaccagt tgcgtgagcg gaaagatggc
     2761 cgcttcccgg ccctgctgca gggagctcaa aatggaggac gcggcgctcg ggagagcggg
     2821 cgggtgagtc acccacacaa aggaaaaggg cctttccgtc ctcagccgtc gcttcatgtg
     2881 actccacgga gtaccgggcg ccgtccaggc acctcgatta gttctcgagc ttttggagta
     2941 cgtcgtcttt aggttggggg gaggggtttt atgcgatgga gtttccccac actgagtggg
     3001 tggagactga agttaggcca gcttggcact tgatgtaatt ctccttggaa tttgcccttt
     3061 ttgagtttgg atcttggttc attctcaagc ctcagacagt ggttcaaagt ttttttcttc
     3121 catttcaggt gtcgtgacaa gtttgtacaa aaaagcaggc tgccaccatg gaacaccagc
     3181 tcctgtgctg cgaagtggaa accatccgcc gcgcgtaccc cgatgccaac ctcctcaacg
     3241 accgggtgct gcgggccatg ctgaaggcgg aggagacctg cgcgccctcg gtgtcctact
     3301 tcaaatgtgt gcagaaggag gtcctgccgt ccatgcggaa gatcgtcgcc acctggatgc
     3361 tggaggtctg cgaggaacag aagtgcgagg aggaggtctt cccgctggcc atgaactacc
     3421 tggaccgctt cctgtcgctg gagcccgtga aaaagagccg cctgcagctg ctgggggcca
     3481 cttgcatgtt cgtggcctct aagatgaagg agaccatccc cctgacggcc gagaagctgt
     3541 gcatctacac cgacaactcc atccggcccg aggagctgct gcaaatggag ctgctcctgg
     3601 tgaacaagct caagtggaac ctggccgcaa tgaccccgca cgatttcatt gaacacttcc
     3661 tctccaaaat gccagaggcg gaggagaaca aacagatcat ccgcaaacac gcgcagacct
     3721 tcgttgccct ctgtgccaca gatgtgaagt tcatttccaa tccgccctcc atggtggcag
     3781 cggggagcgt ggtggccgca gtgcaaggcc tgaacctgag gagccccaac aacttcctgt
     3841 cctactaccg cctcacacgc ttcctctcca gagtgatcaa gtgtgacccg gactgcctcc
     3901 gggcctgcca ggagcagatc gaagccctgc tggagtcaag cctgcgccag gcccagcaga
     3961 acatggaccc caaggccgcc gaggaggagg aagaggagga ggaggaggtg gacctggctt
     4021 gcgcacccac cgacgtgcgg gacgtggaca tctacccata cgatgttcca gattacgctt
     4081 gaacccagct ttcttgtaca aagtggtgat aatcgaattc cgataatcaa cctctggatt
     4141 acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg
     4201 gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct
     4261 cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc
     4321 aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca
     4381 ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac
     4441 tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt
     4501 ccgtggtgtt gtcggggaag ctgacgtcct ttccatggct gctcgcctgt gttgccacct
     4561 ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc
     4621 cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga
     4681 cgagtcggat ctccctttgg gccgcctccc cgcatcggga attcccgcgg ttcgaattct
     4741 accgggtagg ggaggcgctt ttcccaaggc agtctggagc atgcgcttta gcagccccgc
     4801 tgggcacttg gcgctacaca agtggcctct ggcctcgcac acattccaca tccaccggta
     4861 ggcgccaacc ggctccgttc tttggtggcc ccttcgcgcc accttctact cctcccctag
     4921 tcaggaagtt cccccccgcc ccgcagctcg cgtcgtgcag gacgtgacaa atggaagtag
     4981 cacgtctcac tagtctcgtg cagatggaca gcaccgctga gcaatggaag cgggtaggcc
     5041 tttggggcag cggccaatag cagctttgct ccttcgcttt ctgggctcag aggctgggaa
     5101 ggggtgggtc cgggggcggg ctcaggggcg ggctcagggg cggggcgggc gcccgaaggt
     5161 cctccggagg cccggcattc tgcacgcttc aaaagcgcac gtctgccgcg ctgttctcct
     5221 cttcctcatc tccgggcctt tcgacctcac gtggccacca tgaccgagta caagcccacg
     5281 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
     5341 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
     5401 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     5461 gacgacggcg ccgcggtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     5521 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     5581 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     5641 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     5701 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
     5761 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     5821 ccgcgcacct ggtgcatgac ccgcaagccc ggtgcctgag gtacctttaa gaccaatgac
     5881 ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct
     5941 aattcactcc caacgaagac aagatctgct ttttgcttgt actgggtctc tctggttaga
     6001 ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata
     6061 aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta
     6121 gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtagta gttcatgtca
     6181 tcttattatt cagtatttat aacttgcaaa gaaatgaata tcagagagtg agaggaactt
     6241 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa
     6301 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca
     6361 tgtctggctc tagctatccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt
     6421 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc
     6481 gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctagggacg
     6541 tacccaattc gccctatagt gagtcgtatt acgcgcgctc actggccgtc gttttacaac
     6601 gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt
     6661 tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca
     6721 gcctgaatgg cgaatgggac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg
     6781 ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct
     6841 tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc
     6901 ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg
     6961 atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt
     7021 ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg
     7081 tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc
     7141 tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgcttaca atttaggtgg
     7201 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
     7261 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
     7321 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct
     7381 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg
     7441 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg
     7501 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt
     7561 atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga
     7621 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga
     7681 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac
     7741 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg
     7801 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac
     7861 gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct
     7921 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct
     7981 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg
     8041 gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat
     8101 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg
     8161 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat
     8221 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
     8281 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     8341 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     8401 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     8461 gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta
     8521 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     8581 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     8641 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     8701 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     8761 cacgcttccc gaagagagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     8821 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     8881 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     8941 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     9001 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
     9061 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
     9121 ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag
     9181 ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag
     9241 ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg
     9301 tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa
     9361 gcgcgcaatt aaccctcact aaagggaaca aaagctggag ctgcaagctt
//
