LOCUS       Exported               13088 bp DNA     circular SYN 04-JUN-2018
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13088)
  AUTHORS   Triple Threat
  TITLE     Direct Submission
  JOURNAL   Exported Jul 19, 2021 from SnapGene Viewer 5.3.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..13088
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        1..153
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             154..334
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    381..506
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    999..1232
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    1729..1845
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        1868..2171
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2172..2376
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     misc_feature    2467..2475
                     /label=Kozak sequence
     CDS             2476..2532
                     /codon_start=1
                     /label=bpNLS
                     /translation="MKRTADGSEFESPKKKRKV"
     CDS             2533..3216
                     /codon_start=1
                     /label=Anc689 APOBEC
                     /translation="SSETGPVAVDPTLRRRIEPHEFEVFFDPRELRKETCLLYEIKWGT
                     SHKIWRHSSKNTTKHVEVNFIEKFTSERHFCPSTSCSITWFLSWSPCGECSKAITEFLS
                     QHPNVTLVIYVARLYHHMDQQNRQGLRDLVNSGVTIQIMTAPEYDYCWRNFVNYPPGKE
                     AHWPRYPPLWMKLYALELHAGILGLPPCLNILRRKQPQLTFFTIALQSCHYQRLPPHIL
                     WATGLK"
     CDS             3313..7413
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(D10A)
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the D10A mutation in the RuvC catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             7444..7692
                     /codon_start=1
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             7723..7971
                     /codon_start=1
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             7984..8034
                     /codon_start=1
                     /label=bpNLS
                     /translation="KRTADGSEFEPKKKRKV"
     CDS             8044..8100
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             8101..8811
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     misc_feature    8859..9447
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             complement(9330..9341)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             9533..9766
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    9838..9959
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      9999..10134
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        complement(10155..10173)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(10183..10199)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      10341..10796
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        10822..10926
                     /gene="bla"
                     /label=AmpR promoter
     CDS             10927..11787
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      11958..12546
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    12834..12855
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        12870..12900
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12908..12924
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     12932..12948
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        12969..12987
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        13015..13088
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
ORIGIN
        1 aaaaagcacc gtgcatgccg attggtggaa gtaaggtggt acgatcgtgc cttattagga
       61 aggcaacaga cgggtctgac atggattgga cgaaccactg aattgccgca ttgcagagat
      121 attgtattta agtgcctagc tcgatacata aacgggtctc tctggttaga ccagatctga
      181 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      241 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      301 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      361 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      421 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      481 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      541 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      601 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      661 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
      721 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
      781 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
      841 aagcggccgc tgatcttcag acctggagga ggagatatga gggacaattg gagaagtgaa
      901 ttatataaat ataaagtagt aaaaattgaa ccattaggag tagcacccac caaggcaaag
      961 agaagagtgg tgcagagaga aaaaagagca gtgggaatag gagctttgtt ccttgggttc
     1021 ttgggagcag caggaagcac tatgggcgca gcgtcaatga cgctgacggt acaggccaga
     1081 caattattgt ctggtatagt gcagcagcag aacaatttgc tgagggctat tgaggcgcaa
     1141 cagcatctgt tgcaactcac agtctggggc atcaagcagc tccaggcaag aatcctggct
     1201 gtggaaagat acctaaagga tcaacagctc ctggggattt ggggttgctc tggaaaactc
     1261 atttgcacca ctgctgtgcc ttggaatgct agttggagta ataaatctct ggaacagatt
     1321 tggaatcaca cgacctggat ggagtgggac agagaaatta acaattacac aagcttaata
     1381 cactccttaa ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga attattggaa
     1441 ttagataaat gggcaagttt gtggaattgg tttaacataa caaattggct gtggtatata
     1501 aaattattca taatgatagt aggaggcttg gtaggtttaa gaatagtttt tgctgtactt
     1561 tctatagtga atagagttag gcagggatat tcaccattat cgtttcagac ccaccttccc
     1621 aaccccgagg ggacccgaca ggcccgaagg aatagaagaa gaaggtggag agagagacag
     1681 agacagatcc attcgattag tgaacggatc tcgacggtat cggttaactt ttaaaagaaa
     1741 aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag caacagacat
     1801 acaaactaaa gaattacaaa aacaaattac aaaattcaaa attttatcga taagcttggg
     1861 agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc
     1921 gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt
     1981 gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc
     2041 atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg
     2101 cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg
     2161 ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact
     2221 cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa
     2281 atcaacggga ctttccaaaa tgtcgtaaca actccgcccc cattgacgca aatgggcggt
     2341 aggcgtgtac ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagatcgcc
     2401 tggagacgcc atccacgctg ttttgacctc catagaagac accgactcta gaggatccgg
     2461 actagtgccg ccaccatgaa acggacagcc gacggaagcg agttcgagtc accaaagaag
     2521 aagcggaaag tcagcagtga aaccggacca gtggcagtgg acccaaccct gaggagacgg
     2581 attgagcccc atgaatttga agtgttcttt gacccaaggg agctgaggaa ggagacatgc
     2641 ctgctgtacg agatcaagtg gggcacaagc cacaagatct ggcgccacag ctccaagaac
     2701 accacaaagc acgtggaagt gaatttcatc gagaagttta cctccgagcg gcacttctgc
     2761 ccctctacca gctgttccat cacatggttt ctgtcttgga gcccttgcgg cgagtgttcc
     2821 aaggccatca ccgagttcct gtctcagcac cctaacgtga ccctggtcat ctacgtggcc
     2881 cggctgtatc accacatgga ccagcagaac aggcagggcc tgcgcgatct ggtgaattct
     2941 ggcgtgacca tccagatcat gacagcccca gagtacgact attgctggcg gaacttcgtg
     3001 aattatccac ctggcaagga ggcacactgg ccaagatacc cacccctgtg gatgaagctg
     3061 tatgcactgg agctgcacgc aggaatcctg ggcctgcctc catgtctgaa tatcctgcgg
     3121 agaaagcagc cccagctgac atttttcacc attgctctgc agtcttgtca ctatcagcgg
     3181 ctgcctcctc atattctgtg ggctacaggc cttaaatctg gaggatctag cggaggatcc
     3241 tctggcagcg agacaccagg aacaagcgag tcagcaacac cagagagcag tggcggcagc
     3301 agcggcggca gcgacaagaa gtacagcatc ggcctggcca tcggcaccaa ctctgtgggc
     3361 tgggccgtga tcaccgacga gtacaaggtg cccagcaaga aattcaaggt gctgggcaac
     3421 accgaccggc acagcatcaa gaagaacctg atcggagccc tgctgttcga cagcggcgaa
     3481 acagccgagg ccacccggct gaagagaacc gccagaagaa gatacaccag acggaagaac
     3541 cggatctgct atctgcaaga gatcttcagc aacgagatgg ccaaggtgga cgacagcttc
     3601 ttccacagac tggaagagtc cttcctggtg gaagaggata agaagcacga gcggcacccc
     3661 atcttcggca acatcgtgga cgaggtggcc taccacgaga agtaccccac catctaccac
     3721 ctgagaaaga aactggtgga cagcaccgac aaggccgacc tgcggctgat ctatctggcc
     3781 ctggcccaca tgatcaagtt ccggggccac ttcctgatcg agggcgacct gaaccccgac
     3841 aacagcgacg tggacaagct gttcatccag ctggtgcaga cctacaacca gctgttcgag
     3901 gaaaacccca tcaacgccag cggcgtggac gccaaggcca tcctgtctgc cagactgagc
     3961 aagagcagac ggctggaaaa tctgatcgcc cagctgcccg gcgagaagaa gaatggcctg
     4021 ttcggaaacc tgattgccct gagcctgggc ctgaccccca acttcaagag caacttcgac
     4081 ctggccgagg atgccaaact gcagctgagc aaggacacct acgacgacga cctggacaac
     4141 ctgctggccc agatcggcga ccagtacgcc gacctgtttc tggccgccaa gaacctgtcc
     4201 gacgccatcc tgctgagcga catcctgaga gtgaacaccg agatcaccaa ggcccccctg
     4261 agcgcctcta tgatcaagag atacgacgag caccaccagg acctgaccct gctgaaagct
     4321 ctcgtgcggc agcagctgcc tgagaagtac aaagagattt tcttcgacca gagcaagaac
     4381 ggctacgccg gctacattga cggcggagcc agccaggaag agttctacaa gttcatcaag
     4441 cccatcctgg aaaagatgga cggcaccgag gaactgctcg tgaagctgaa cagagaggac
     4501 ctgctgcgga agcagcggac cttcgacaac ggcagcatcc cccaccagat ccacctggga
     4561 gagctgcacg ccattctgcg gcggcaggaa gatttttacc cattcctgaa ggacaaccgg
     4621 gaaaagatcg agaagatcct gaccttccgc atcccctact acgtgggccc tctggccagg
     4681 ggaaacagca gattcgcctg gatgaccaga aagagcgagg aaaccatcac cccctggaac
     4741 ttcgaggaag tggtggacaa gggcgcttcc gcccagagct tcatcgagcg gatgaccaac
     4801 ttcgataaga acctgcccaa cgagaaggtg ctgcccaagc acagcctgct gtacgagtac
     4861 ttcaccgtgt ataacgagct gaccaaagtg aaatacgtga ccgagggaat gagaaagccc
     4921 gccttcctga gcggcgagca gaaaaaggcc atcgtggacc tgctgttcaa gaccaaccgg
     4981 aaagtgaccg tgaagcagct gaaagaggac tacttcaaga aaatcgagtg cttcgactcc
     5041 gtggaaatct ccggcgtgga agatcggttc aacgcctccc tgggcacata ccacgatctg
     5101 ctgaaaatta tcaaggacaa ggacttcctg gacaatgagg aaaacgagga cattctggaa
     5161 gatatcgtgc tgaccctgac actgtttgag gacagagaga tgatcgagga acggctgaaa
     5221 acctatgccc acctgttcga cgacaaagtg atgaagcagc tgaagcggcg gagatacacc
     5281 ggctggggca ggctgagccg gaagctgatc aacggcatcc gggacaagca gtccggcaag
     5341 acaatcctgg atttcctgaa gtccgacggc ttcgccaaca gaaacttcat gcagctgatc
     5401 cacgacgaca gcctgacctt taaagaggac atccagaaag cccaggtgtc cggccagggc
     5461 gatagcctgc acgagcacat tgccaatctg gccggcagcc ccgccattaa gaagggcatc
     5521 ctgcagacag tgaaggtggt ggacgagctc gtgaaagtga tgggccggca caagcccgag
     5581 aacatcgtga tcgaaatggc cagagagaac cagaccaccc agaagggaca gaagaacagc
     5641 cgcgagagaa tgaagcggat cgaagagggc atcaaagagc tgggcagcca gatcctgaaa
     5701 gaacaccccg tggaaaacac ccagctgcag aacgagaagc tgtacctgta ctacctgcag
     5761 aatgggcggg atatgtacgt ggaccaggaa ctggacatca accggctgtc cgactacgat
     5821 gtggaccata tcgtgcctca gagctttctg aaggacgact ccatcgacaa caaggtgctg
     5881 accagaagcg acaagaaccg gggcaagagc gacaacgtgc cctccgaaga ggtcgtgaag
     5941 aagatgaaga actactggcg gcagctgctg aacgccaagc tgattaccca gagaaagttc
     6001 gacaatctga ccaaggccga gagaggcggc ctgagcgaac tggataaggc cggcttcatc
     6061 aagagacagc tggtggaaac ccggcagatc acaaagcacg tggcacagat cctggactcc
     6121 cggatgaaca ctaagtacga cgagaatgac aagctgatcc gggaagtgaa agtgatcacc
     6181 ctgaagtcca agctggtgtc cgatttccgg aaggatttcc agttttacaa agtgcgcgag
     6241 atcaacaact accaccacgc ccacgacgcc tacctgaacg ccgtcgtggg aaccgccctg
     6301 atcaaaaagt accctaagct ggaaagcgag ttcgtgtacg gcgactacaa ggtgtacgac
     6361 gtgcggaaga tgatcgccaa gagcgagcag gaaatcggca aggctaccgc caagtacttc
     6421 ttctacagca acatcatgaa ctttttcaag accgagatta ccctggccaa cggcgagatc
     6481 cggaagcggc ctctgatcga gacaaacggc gaaaccgggg agatcgtgtg ggataagggc
     6541 cgggattttg ccaccgtgcg gaaagtgctg agcatgcccc aagtgaatat cgtgaaaaag
     6601 accgaggtgc agacaggcgg cttcagcaaa gagtctatcc tgcccaagag gaacagcgat
     6661 aagctgatcg ccagaaagaa ggactgggac cctaagaagt acggcggctt cgacagcccc
     6721 accgtggcct attctgtgct ggtggtggcc aaagtggaaa agggcaagtc caagaaactg
     6781 aagagtgtga aagagctgct ggggatcacc atcatggaaa gaagcagctt cgagaagaat
     6841 cccatcgact ttctggaagc caagggctac aaagaagtga aaaaggacct gatcatcaag
     6901 ctgcctaagt actccctgtt cgagctggaa aacggccgga agagaatgct ggcctctgcc
     6961 ggcgaactgc agaagggaaa cgaactggcc ctgccctcca aatatgtgaa cttcctgtac
     7021 ctggccagcc actatgagaa gctgaagggc tcccccgagg ataatgagca gaaacagctg
     7081 tttgtggaac agcacaagca ctacctggac gagatcatcg agcagatcag cgagttctcc
     7141 aagagagtga tcctggccga cgctaatctg gacaaagtgc tgtccgccta caacaagcac
     7201 cgggataagc ccatcagaga gcaggccgag aatatcatcc acctgtttac cctgaccaat
     7261 ctgggagccc ctgccgcctt caagtacttt gacaccacca tcgaccggaa gaggtacacc
     7321 agcaccaaag aggtgctgga cgccaccctg atccaccaga gcatcaccgg cctgtacgag
     7381 acacggatcg acctgtctca gctgggaggt gacagcggcg ggagcggcgg gagcgggggg
     7441 agcactaatc tgagcgacat cattgagaag gagactggga aacagctggt cattcaggag
     7501 tccatcctga tgctgcctga ggaggtggag gaagtgatcg gcaacaagcc agagtctgac
     7561 atcctggtgc acaccgccta cgacgagtcc acagatgaga atgtgatgct gctgacctct
     7621 gacgcccccg agtataagcc ttgggccctg gtcatccagg attctaacgg cgagaataag
     7681 atcaagatgc tgagcggagg atccggagga tctggaggca gcaccaacct gtctgacatc
     7741 atcgagaagg agacaggcaa gcagctggtc atccaggaga gcatcctgat gctgcccgaa
     7801 gaagtcgaag aagtgatcgg aaacaagcct gagagcgata tcctggtcca taccgcctac
     7861 gacgagagta ccgacgaaaa tgtgatgctg ctgacatccg acgccccaga gtataagccc
     7921 tgggctctgg tcatccagga ttccaacgga gagaacaaaa tcaaaatgct gtctggcggc
     7981 tcaaaaagaa ccgccgacgg cagcgaattc gagcccaaga agaagaggaa agtcggaagc
     8041 ggagctacta acttcagcct gctgaagcag gctggagacg tggaggagaa ccctggacct
     8101 atggtgagca agggcgagga ggataacatg gccatcatca aggagttcat gcgcttcaag
     8161 gtgcacatgg agggctccgt gaacggccac gagttcgaga tcgagggcga gggcgagggc
     8221 cgcccctacg agggcaccca gaccgccaag ctgaaggtga ccaagggtgg ccccctgccc
     8281 ttcgcctggg acatcctgtc ccctcagttc atgtacggct ccaaggccta cgtgaagcac
     8341 cccgccgaca tccccgacta cttgaagctg tccttccccg agggcttcaa gtgggagcgc
     8401 gtgatgaact tcgaggacgg cggcgtggtg accgtgaccc aggactcctc cctgcaggac
     8461 ggcgagttca tctacaaggt gaagctgcgc ggcaccaact tcccctccga cggccccgta
     8521 atgcagaaga agaccatggg ctgggaggcc tcctccgagc ggatgtaccc cgaggacggc
     8581 gccctgaagg gcgagatcaa gcagaggctg aagctgaagg acggcggcca ctacgacgct
     8641 gaggtcaaga ccacctacaa ggccaagaag cccgtgcagc tgcccggcgc ctacaacgtc
     8701 aacatcaagt tggacatcac ctcccacaac gaggactaca ccatcgtgga acagtacgaa
     8761 cgcgccgagg gccgccactc caccggcggc atggacgagc tgtacaagta agctaagcac
     8821 ttcgtggccg aggagcagga ctgagaattc cagtcgacaa tcaacctctg gattacaaaa
     8881 tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg
     8941 ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct
     9001 tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg
     9061 gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct
     9121 gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg
     9181 ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg
     9241 tgttgtcggg gaagctgacg tcctttccat ggctgctcgc ctgtgttgcc acctggattc
     9301 tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc
     9361 gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc
     9421 ggatctccct ttgggccgcc tccccgcctg gaattcgagc tcggtacctt taagaccaat
     9481 gacttacaag gcagctgtag atcttagcca ctttttaaag aaaagggggg actggaaggg
     9541 ctaattcact cccaacgaag acaagatctg ctttttgctt gtactgggtc tctctggtta
     9601 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa
     9661 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac
     9721 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtag tagttcatgt
     9781 catcttatta ttcagtattt ataacttgca aagaaatgaa tatcagagag tgagaggaac
     9841 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat
     9901 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat
     9961 catgtctggc tctagctatc ccgcccctaa ctccgcccat cccgccccta actccgccca
    10021 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
    10081 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggga
    10141 cgtacccaat tcgccctata gtgagtcgta ttacgcgcgc tcactggccg tcgttttaca
    10201 acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc
    10261 tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg
    10321 cagcctgaat ggcgaatggg acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt
    10381 ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt
    10441 cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct
    10501 ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg
    10561 tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga
    10621 gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc
    10681 ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga
    10741 gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgctta caatttaggt
    10801 ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca
    10861 aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg
    10921 aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc
    10981 cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg
    11041 ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt
    11101 cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta
    11161 ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat
    11221 gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga
    11281 gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca
    11341 acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact
    11401 cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc
    11461 acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact
    11521 ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt
    11581 ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt
    11641 gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt
    11701 atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata
    11761 ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag
    11821 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat
    11881 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
    11941 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
    12001 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
    12061 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg
    12121 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
    12181 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
    12241 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
    12301 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
    12361 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
    12421 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
    12481 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
    12541 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
    12601 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag
    12661 tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa
    12721 gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc
    12781 agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg
    12841 agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg
    12901 tgtggaattg tgagcggata acaatttcac acaggaaaca gctatgacca tgattacgcc
    12961 aagcgcgcaa ttaaccctca ctaaagggaa caaaagctgg agctgcaagc ttaatgtagt
    13021 cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa catgccttac
    13081 aaggagag
//
