LOCUS       11942-M05-950          13322 bp    DNA     circular SYN 20-DEC-2011
DEFINITION  85xpona-CFP-24xPP7-loxP IRES-DsRed
ACCESSION   11942-M05-950
KEYWORDS    .
SOURCE      Unknown.
  ORGANISM  Unknown
            Unclassified.
REFERENCE   1  (bases 1 to 13322)
  AUTHORS   Self
  JOURNAL   Unpublished.
COMMENT     SECID/File created by Clone Manager, Scientific & Educational Software
COMMENT     SECNOTES|Mammalian multisite expression clone from C455-E01 (mini 
            stuffer), R985-E06 (24xPP7-loxP) and C122-E25 (IRES DsRed) in 
            pDest-950 (85xpona-CFP).
FEATURES             Location/Qualifiers
     misc_feature    1..636
                     /gene="5'LTR"
                     /SECDrawAs="Region"
                     /SECStyleId="1"
     misc_feature    637..1030
                     /gene="PSI"
                     /SECDrawAs="Region"
                     /SECStyleId="1"
     misc_feature    1031..1536
                     /gene="RRE"
                     /SECDrawAs="Region"
                     /SECStyleId="1"
     misc_feature    2034..2211
                     /gene="TAR"
                     /SECDrawAs="Region"
                     /SECStyleId="1"
     CDS             6217..7056
                     /dnas_title="beta-lactamase (mature form)"
                     /gene="AMPr"
                     /product="beta-lactamase (mature form)"
                     /SECDrawAs="Gene"
                     /SECStyleId="1"
     misc_feature    1..636
                     /note="5'-LTR"
     misc_feature    637..1030
                     /note="PSI"
     misc_feature    1031..1536
                     /note="RRE"
     misc_feature    2034..2211
                     /note="TAR"
     misc_feature    6100..7056
                     /note="Ampicillin R"
     misc_feature    8353..8423
                     /note="SV40 PolyA Signal"
     CDS             2213..2217
                     /gene="85xpona"
                     /SECDrawAs="Gene"
                     /SECStyleId="1"
     misc_feature    complement(3816..4043)
                     /note="bGH_PA_Terminator"
     misc_feature    3754..3781
                     /note="#1335"
     misc_feature    3782..3815
                     /note="FRT"
     misc_feature    complement(4078..4476)
                     /note="Blasticidin"
     misc_feature    complement(4486..4539)
                     /label=2A
                     /SerialCloner_Color="&h00FF00"
                     /SerialCloner_Show="True"
                     /SerialCloner_Protect="True"
                     /SerialCloner_Arrow="True"
     PCR_primer      4474..4492
                     /pair=""
                     /primer="CATGGCGCGCCTAGGGCCG"
                     /current=0
     PCR_primer      complement(4479..4493)
                     /pair=""
                     /primer="CCGGCCCTAGGCGCG"
                     /current=0
     misc_feature    complement(4540..5487)
                     /note="iRFP"
     PCR_primer      complement(4536..4557)
                     /pair=""
                     /primer="ATCGGCGTGATGGAAGAGGAGG  0431#"
                     /current=0
     misc_feature    complement(5491..5515)
                     /label=SA
                     /SerialCloner_Color="&hFF0000"
                     /SerialCloner_Show="True"
                     /SerialCloner_Protect="True"
                     /SerialCloner_Arrow="True"
     PCR_primer      complement(5247..5266)
                     /pair=""
                     /primer="CCGTCTGACGATCGCCTTGG  1431#"
                     /current=0
     misc_feature    5516..5549
                     /note="FRT"
     misc_feature    5516..5519
                     /note="1127 F"
     misc_feature    5550..5574
                     /note="#1336"
     PCR_primer      complement(5946..5966)
                     /pair=""
                     /primer="CCCGGGCCTCTCACTCTCTGA  2431#"
                     /current=0
     misc_feature    5655..5889
                     /note="3'LTR"
     PCR_primer      complement(5955..5978)
                     /pair=""
                     /primer="TTCCTTAATTAACCCGGGCCTCTC 9341#"
                     /current=0
     PCR_primer      5570..5598
                     /pair=""
                     /primer="#1438 CGCTCTGTACACCTGAGATCCTTTAAGAC"
                     /current=0
     CDS             complement(2246..3739)
                     /gene="24xPP7"
                     /SECDrawAs="Gene"
                     /SECStyleId="1"
                     /SECName="24xPP7"
     PCR_primer      5661..5685
                     /pair=""
                     /primer="#1015 GGGCTAATTCACTCCCAACGAAGAC"
                     /current=1
     source          1..8603
                     /dnas_title="iRFP_Bsd_PP7_LentiViral_Vector"
ORIGIN      
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtACC
     2221 GGTGCTAGCt gatatctgtC AATTGcacgc gttctcgaga tctaatgaac ccgggaatac
     2281 tgcagccagc gagcccatat gctctgctgg tttctagagt cgacctgcag ggagcgacgc
     2341 catatcgtct gctcctttct aggcaattag gtaccttagg atctaatgaa cccgggaata
     2401 ctgcagccag cgagcccata tgctctgctg gtttctagag tcgacctgca gggagcgacg
     2461 ccatatcgtc tgctcctttc taggcaatta ggtaccttag gatctaatga acccgggaat
     2521 actgcagcca gcgagcccat atgctctgct ggtttctaga gtcgacctgc agggagcgac
     2581 gccatatcgt ctgctccttt ctaggcaatt aggtacctta ggatctaatg aacccgggaa
     2641 tactgcagcc agcgagccca tatgctctgc tggtttctag agtcgacctg cagggagcga
     2701 cgccatatcg tctgctcctt tctaggcaat taggtacctt aggatctaat gaacccggga
     2761 atactgcagc cagcgagccc atatgctctg ctggtttcta gagtcgacct gcagggagcg
     2821 acgccatatc gtctgctcct ttctaggcaa ttaggtacct taggatctaa tgaacccggg
     2881 aatactgcag ccagcgagcc catatgctct gctggtttct agagtcgacc tgcagggagc
     2941 gacgccatat cgtctgctcc tttctaggca attaggtacc ttaggatcta atgaacccgg
     3001 gaatactgca gccagcgagc ccatatgctc tgctggtttc tagagtcgac ctgcagggag
     3061 cgacgccata tcgtctgctc ctttctaggc aattaggtac cttaggatct aatgaacccg
     3121 ggaatactgc agccagcgag cccatatgct ctgctggttt ctagagtcga cctgcaggga
     3181 gcgacgccat atcgtctgct cctttctagg caattaggta ccttaggatc taatgaaccc
     3241 gggaatactg cagccagcga gcccatatgc tctgctggtt tctagagtcg acctgcaggg
     3301 agcgacgcca tatcgtctgc tcctttctag gcaattaggt accttaggat ctaatgaacc
     3361 cgggaatact gcagccagcg agcccatatg ctctgctggt ttctagagtc gacctgcagg
     3421 gagcgacgcc atatcgtctg ctcctttcta ggcaattagg taccttagga tctaatgaac
     3481 ccgggaatac tgcagccagc gagcccatat gctctgctgg tttctagagt cgacctgcag
     3541 ggagcgacgc catatcgtct gctcctttct aggcaattag gtaccttagg atctaatgaa
     3601 cccgggaata ctgcagccag cgagcccata tgctctgctg gtttctagag tcgacctgca
     3661 gggagcgacg ccatatcgtc tgctcctttc taggcaatta ggtaccttag gatctcgagt
     3721 atcgatacat tacactttaa cccactttgt atacaactct tgtgtagcga ttatggatgg
     3781 cGAAGTTCCT ATTCTCTAGA AAGTATAGGA ACTTCccata gagcccaccg catccccagc
     3841 atgcctgcta ttgtcttccc aatcctcccc cttgctgtcc tgccccaccc caccccccag
     3901 aatagaatga cacctactca gacaatgcga tgcaatttcc tcattttatt aggaaaggac
     3961 agtgggagtg gcaccttcca gggtcaagga aggcacgggg gaggggcaaa caacagatgg
     4021 ctggcaacta gaaggcacag tcgaggctga tcagcgggtt taaacgggcc ctctagatca
     4081 GCCCTCCCAC ACATAACCAG AGGGCAGCAA TTCACGAATC CCAACTGCCG TCGGCTGTCC
     4141 ATCACTGTCC TTCACTATGG CTTTGATCCC AGGATGCAGA TCGAGAAGCA CCTGTCGGCA
     4201 CCGTCCGCAG GGGCTCAAGA TGCCCCTGTT CTCATTTCCG ATCGCGACGA TACAAGTCAG
     4261 GTTGCCTGCA GCCGCAGCAG CAGCAGTGCC CAGCACCACG AGTTCTGCAC AAGGTCCCCC
     4321 AGTAAAATGA TATACATTGA CACCAGTGAA GATGCGGCCG TCGCTAGAGA GAGCTGCGCT
     4381 GGCGACGCTG TAGTCTTCAG AGATGGGGAT GCTGTTGATT GTAGCCGTTG CTCTTTCAAT
     4441 GAGGGTGGAT TCTTCTTGAG ACAAAGGCTT GGCCATggcg cgcctagggc cgggattctc
     4501 ctccacgtca ccgcatgtta gaagacttcc tctgccctcc tcttccatca cgccgatctg
     4561 ccaggccaga acctgggcga ctagctcgca ggcttggcgg ccatcgagat cgacgtagta
     4621 cggcgttcgg tgatggcaaa cgatcaatcc ccacagtcgc tcgccgcgca aaatcgagat
     4681 cgacatcgtg ccgtgcatgc ctatgttgcg catgaattcc agatggacgg gcgagacgct
     4741 gcgcaggatg gcgaagctaa gatcaatcgg ccgcccggtg accggattga ggtctggggt
     4801 gaccggcacc ggccgataat tgatatcggg aatgatccgt accgggttga tggtatagag
     4861 ccgacgggcc tgcgccggca cggttgaggc aggatagtgc aggcctagtt ttgactcgac
     4921 ctcggcgcac cgatcctctg cgatcacttc gccgctgaag tcggaggcga agcgatagat
     4981 catcacccga tcgaagccgg taatcttccg cacctcttgc gccgcggcgg cgcaggcgct
     5041 ttccaaggtt tcggcggcct gcaggcggcg gatggcgctg ttggtgcggc ggaagaacgc
     5101 ctgcggctcg gcgacgtccc gctggggagg ctcgagctcg aggaagatga gctgatcatg
     5161 gcgatgccag gagccgatga agcctgcgtc ctttcgcatc gtgaagccga cagtgatcgg
     5221 tgctccgacg gccgccccgg gctcggccaa ggcgatcgtc agacggttgt gcgtctccga
     5281 gtcgaagaca tcggccgcag agcggccgat cagggcgccg atcgccagtc cggtgagttc
     5341 gggaaggttg tcgctgccgg caacgatcgt catgtcggcg gcgagggcga gcagcagtcc
     5401 atgcggttgg atggcaccgg ggatatggat cggctcatcg tcgcaggtca agaggtcagg
     5461 ctgcctggcg acggatcctt cagccatgcc ctgtgggagg aagagaagag gtcagGAAGT
     5521 TCCTATTCTC TAGAAAGTAT AGGAACTTCg ataaatgccc agattctgcc gctcTGTACA
     5581 CCTGAGATCC TTTAAGACCA ATGACTTACA AGGcagctgt agatcttagc cactttttaa
     5641 aagaaaaggg gggactggaa gggctaattc actcccaacg aagacaagat ctgctttttg
     5701 cttgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag
     5761 ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc
     5821 gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa
     5881 tctctagcag tagtagttca tgtcatctta ttattcagta tttataactt gcaaagaaat
     5941 gaatatcaga gagtgagagg cccgggttaa ttaaggaaag ggctagatca ttcttgaaga
     6001 cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct
     6061 tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc
     6121 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa
     6181 tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt
     6241 gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct
     6301 gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc
     6361 cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta
     6421 tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac
     6481 tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc
     6541 atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac
     6601 ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg
     6661 gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac
     6721 gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc
     6781 gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt
     6841 gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga
     6901 gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc
     6961 cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag
     7021 atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca
     7081 tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc
     7141 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
     7201 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
     7261 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
     7321 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt
     7381 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
     7441 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
     7501 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
     7561 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
     7621 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
     7681 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
     7741 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
     7801 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
     7861 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt
     7921 accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca
     7981 gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg
     8041 attcattaat gcagcaagct catggctgac taattttttt tatttatgca gaggccgagg
     8101 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     8161 tttgcaaaaa gctccccgtg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc
     8221 aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt
     8281 ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat
     8341 gacatgatta cgaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg
     8401 tccaaactca tcaatgtatc ttatcatgtc tggatcaact ggataactca agctaaccaa
     8461 aatcatccca aacttcccac cccataccct attaccactg ccaattacct gtggtttcat
     8521 ttactctaaa cctgtgattc ctctgaatta ttttcatttt aaagaaattg tatttgttaa
     8581 atatgtacta caaacttagt agt
//

