LOCUS       pDGO186N-KS3-1219neo      4423 bp    DNA     circular UNA 28-SEP-2010
DEFINITION  
ACCESSION   pMU102
DBSOURCE    GenBank
SOURCE      Cloning vector pNW33N (unknown)
  ORGANISM  Cloning vector pNW33N
            Cloning vector pNW33N
            other sequences; artificial sequences; vectors
FEATURES             Location/Qualifiers
     Replication_ori 1..589
                     /note="Oriinal location: complement(2766..3354)"
                     /note="Escherichia coli origin of replication from
                     plasmid pUC19"
                     /label="E. coli origin of replication"
     primer          complement(22..41)
                     /label="TY112"
     primer          complement(56..73)
                     /label="X03373"
     primer          692..716
                     /label="BB R2 int F"
     primer          complement(701..725)
                     /label="BB F int R"
     Replication_ori 826..1280
                     /label="C. therm origin of replication"
     CDS             1281..2285
                     /gene="repB"
                     /codon_start=1
                     /transl_table=11
                     /product="RepB"
                     /protein_id="AAO86061.1"
                     /db_xref="GI:29650180"
                     /note="Oriinal location: complement(1070..2074)"
                     /note="replication protein; rolling circle replication
                     initiation protein; Bacillus origin of replication from
                     Geobacillus stearothermophilus plasmid pTHT15"
     gene            1281..2285
                     /gene="repB"
                     /note="Oriinal location: complement(1070..2074)"
     primer          complement(2161..2190)
                     /label="TY113R+"
     primer          2161..2190
                     /label="TY113+"
     primer          2171..2190
                     /label="TY113"
     primer          complement(2171..2190)
                     /label="TY113R"
     primer          complement(2256..2310)
                     /label="Backbone Reverse"
     primer          complement(2266..2285)
                     /label="Cas operon R"
     primer          complement(2272..2296)
                     /label="184N R"
     primer          2286..2331
                     /label="CRISPR Forward"
     gblock          2286..2663
                     /label="gblock#1"
     primer          2297..2316
                     /label="Cas operon F"
     promoter        2297..2596
                     /label="1194 promoter"
     repeat          2597..2626
                     /label="repeat#4"
     spacer          2627..2663
                     /label="1219 spacer3"
     gblock          2627..3142
                     /label="gblock#2"
     repeat          2664..2693
                     /label="repeat#5"
     terminator      2694..2933
                     /label="repeat#49 terminator"
     primer          complement(2924..2953)
                     /label="term R"
     primer          complement(2950..3004)
                     /label="Backbone R1"
     primer          complement(2960..3019)
                     /label="Backbone R2"
     primer          complement(2975..3004)
                     /label="SR"
     primer          3005..3064
                     /label="Backbone Forward"
     primer          complement(3005..3031)
                     /label="nheI pyrF R"
     misc_feature    complement(<3005..4209)
                     /feature_type="Insertion"
                     /note="Region on donor sequence (pMU104): 1..3564"
                     /label="pMU104"
     insulator       3005..3031
     promoter        3041..3069
                     /label="CAT promoter"
     primer          complement(3103..3132)
                     /label="TY1B R"
     primer          3113..3172
                     /label="TY1C F"
     primer          3133..3153
                     /label="neoF1"
     primer          3133..3172
                     /label="TY1C F1"
     CDS             3133..3897
                     /label="neomycin"
     gene            3133..3897
                     /label="neomycin"
     primer          complement(3858..3917)
                     /label="TY1C R"
     primer          complement(3877..3897)
                     /label="neoR1"
     primer          3898..3927
                     /label="TY1A F"
     promoter        4063..4099
                     /label="pLac"
     -35_signal      4063..4068
                     /label="pLac -35"
     -10_signal      4087..4092
                     /label="pLac -10"
     Primer_binding_ 4152..4166
                     /label="PstI in-fusion primer binding site F"
     primer          complement(4159..4183)
                     /label="TC01"
     Primer_binding_ 4168..4182
                     /label="in-fusion XbaI primer binding site"
     primer          4193..4217
                     /label="TC02"
ORIGIN
        1 TTGAGATCCT TTTTTTCTGC GCGTAATCTG CTGCTTGCAA ACAAAAAAAC CACCGCTACC
       61 AGCGGTGGTT TGTTTGCCGG ATCAAGAGCT ACCAACTCTT TTTCCGAAGG TAACTGGCTT
      121 CAGCAGAGCG CAGATACCAA ATACTGTTCT TCTAGTGTAG CCGTAGTTAG GCCACCACTT
      181 CAAGAACTCT GTAGCACCGC CTACATACCT CGCTCTGCTA ATCCTGTTAC CAGTGGCTGC
      241 TGCCAGTGGC GATAAGTCGT GTCTTACCGG GTTGGACTCA AGACGATAGT TACCGGATAA
      301 GGCGCAGCGG TCGGGCTGAA CGGGGGGTTC GTGCACACAG CCCAGCTTGG AGCGAACGAC
      361 CTACACCGAA CTGAGATACC TACAGCGTGA GCTATGAGAA AGCGCCACGC TTCCCGAAGG
      421 GAGAAAGGCG GACAGGTATC CGGTAAGCGG CAGGGTCGGA ACAGGAGAGC GCACGAGGGA
      481 GCTTCCAGGG GGAAACGCCT GGTATCTTTA TAGTCCTGTC GGGTTTCGCC ACCTCTGACT
      541 TGAGCGTCGA TTTTTGTGAT GCTCGTCAGG GGGGCGGAGC CTATGGAAAA ACGCCAGCAA
      601 CGCGGCCTTT TTACGGTTCC TGGCCTTTTG CTGGCCTTTT GCTCACATGT TCTTTCCTGC
      661 GTTATCCCCT GATTCTGTGG ATAACCGTAT TACCGCCTTT GAGTGAGCTG ATACCGCTCG
      721 CCGCAGCCGA ACGACCGAGC GCAGCGAGTC AGTGAGCGAG GAAGCGGAAG AGCGCCCAAT
      781 ACGCAAACCG CCTCTCCCCG CGCGTTGGCC GATTCATTAA TGCAGTATGG GAAACAAAAT
      841 ATTGCGTATG CGACTGTTCA CATGGACGAG AAAACCCCTC ACATGCATTT AGGAGTTGTT
      901 CCTATGCGCC TAGAGGGCTT TTCGTGCGTC AGCATGAGCG ATCGGAAGAA AAGAAGAATA
      961 CTTTTTCGCT TCAAGATGTT TTGCAGCGTG ATCGAGAACT TCGTGAGCAA AGAAAAGCAA
     1021 AGAGGAAAAA ATCGCATGAT TTGGAGCGAT AAGAAAAAGC ACTCGAATGA GTGCTTTTTT
     1081 TGCGTTTTGA GCGTAGCGAA AAACGAGTTC TTTCTATTCT TGATACATAT AGAAATAACG
     1141 TCATTTTTAT TTTAGTTGCT GAAAGGTGCG TTGAAGTGTT GGTATGTATG TGTTTTAAAG
     1201 TATTGAAAAC CCTTAAAATT GGTTGCACAG AAAAACCCCA TCTGTTAAAG TTATAAGTGA
     1261 CCAAACAAAT AACTAAATAG ATGGGGGTTT CTTTTAATAT TATGTGTCCT AATAGTAGCA
     1321 TTTATTCAGA TGAAAAATCA AGGGTTTTAG TGGACAAGAC AAAAAGTGGA AAAGTGAGAC
     1381 CATGGAGAGA AAAGAAAATC GCTAATGTTG ATTACTTTGA ACTTCTGCAT ATTCTTGAAT
     1441 TTAAAAAGGC TGAAAGAGTA AAAGATTGTG CTGAAATATT AGAGTATAAA CAAAATCGTG
     1501 AAACAGGCGA AAGAAAGTTG TATCGAGTGT GGTTTTGTAA ATCCAGGCTT TGTCCAATGT
     1561 GCAACTGGAG GAGAGCAATG AAACATGGCA TTCAGTCACA AAAGGTTGTT GCTGAAGTTA
     1621 TTAAACAAAA GCCAACAGTT CGTTGGTTGT TTCTCACATT AACAGTTAAA AATGTTTATG
     1681 ATGGCGAAGA ATTAAATAAG AGTTTGTCAG ATATGGCTCA AGGATTTCGC CGAATGATGC
     1741 AATATAAAAA AATTAATAAA AATCTTGTTG GTTTTATGCG TGCAACGGAA GTGACAATAA
     1801 ATAATAAAGA TAATTCTTAT AATCAGCACA TGCATGTATT GGTATGTGTG GAACCAACTT
     1861 ATTTTAAGAA TACAGAAAAC TACGTGAATC AAAAACAATG GATTCAATTT TGGAAAAAGG
     1921 CAATGAAATT AGACTATGAT CCAAATGTAA AAGTTCAAAT GATTCGACCG AAAAATAAAT
     1981 ATAAATCGGA TATACAATCG GCAATTGACG AAACTGCAAA ATATCCTGTA AAGGATACGG
     2041 ATTTTATGAC CGATGATGAA GAAAAGAATT TGAAACGTTT GTCTGATTTG GAGGAAGGTT
     2101 TACACCGTAA AAGGTTAATC TCCTATGGTG GTTTGTTAAA AGAAATACAT AAAAAATTAA
     2161 ACCTTGATGA CACAGAAGAA GGCGATTTGA TTCATACAGA TGATGACGAA AAAGCCGATG
     2221 AAGATGGATT TTCTATTATT GCAATGTGGA ATTGGGAACG GAAAAATTAT TTTATTAAAG
     2281 AGTAGTTCAA GGATCCTTAA TATGCCGACC ACGTTGCAAT TCCCGTCAAA TAATGCATTT
     2341 TGCAGCCGAC GAAACAGGCA AGATAACTGT ATTGGCTATA AATGTTTCAG GCAGCGGTAT
     2401 ATTTTGCCTC CCGGTAAAAT TAATACAATA AGCTAAAAAA CTGACGTAGG ATAAGCAAAA
     2461 CGGCGCAATT TGAGTTGTAA CGTAATATTT TCACTAAAAA TAGTAATTAT TTCATGTTGT
     2521 TTTTTTTTAG ATTAATTTAT AATATAATTT ATTGTATAAG CAATATCTTA ATTATCATTA
     2581 AAGGGGGAAA AAAACTGTTT GTATCGTACC TATGAGGAAT TGAAACAGAA TCGCACGGTA
     2641 AAGCTCTCCC GCTTCACACG CACGTTTGTA TCGTACCTAT GAGGAATTGA AACTTTCTGG
     2701 CTACTCCAAG TTCCTAATGT TATATATGGT TTTATAGTAT AAAGCCTTAA GCGAAAATGA
     2761 ATAGGACTGT ATAATAAGGT TAAGGCTTTA TATTCGCCTG AAGCGTAAAA ACGCAATAGG
     2821 AAGAATAAAA TTTTTGCCGG GGATAGGCCA GCCTTGCAAA CTGGCTGACA AGCGAAGTCT
     2881 CCCCGCTTCC CCGGCAACAA TTTCAAATAG GGAGACAGAA AGGGAGACGA AATCTCGTAT
     2941 GTTGTGTGGA ATTGTGAGCG GATAACAATT TCACACAGGA AACAGCTATG ACCATGATTA
     3001 CGCCTAATAG AAATAATTTT GTTTAACTTT ACAAACGGGA TTGACTTTTA AAAAAGGATT
     3061 GATTCTAATG AAGAAAGCAG ACAAGTAAGC CTCCTAAATT CACTTTAGAT AAAAATTTAG
     3121 GAGGCATATC AAATGGTGAA TGGACCAATA ATAATGACTA GAGAAGAAAG AATGAAGATT
     3181 GTTCATGAAA TTAAGGAACG AATATTGGAT AAATATGGGG ATGATGTTAA GGCTATTGGT
     3241 GTTTATGGCT CTCTTGGTCG TCAGACTGAT GGGCCCTATT CGGATATTGA GATGATGTGT
     3301 GTCATGTCAA CAGAGGAAGC AGAGTTCAGC CATGAATGGA CAACCGGTGA GTGGAAGGTG
     3361 GAAGTGAATT TTGATAGCGA AGAGATTCTA CTAGATTATG CATCTCAGGT GGAATCAGAT
     3421 TGGCCGCTTA CACATGGTCA ATTTTTCTCT ATTTTGCCGA TTTATGATTC AGGTGGATAC
     3481 TTAGAGAAAG TGTATCAAAC TGCTAAATCG GTAGAAGCCC AAACGTTCCA CGATGCGATT
     3541 TGTGCCCTTA TCGTAGAAGA GCTGTTTGAA TATGCAGGCA AATGGCGTAA TATTCGTGTG
     3601 CAAGGACCGA CAACATTTCT ACCATCCTTG ACTGTACAGG TAGCAATGGC AGGTGCCATG
     3661 TTGATTGGTC TGCATCATCG CATCTGTTAT ACGACGAGCG CTTCGGTCTT AACTGAAGCA
     3721 GTTAAGCAAT CAGATCTTCC TTCAGGTTAT GACCATCTGT GCCAGTTCGT AATGTCTGGT
     3781 CAACTTTCCG ACTCTGAGAA ACTTCTGGAA TCGCTAGAGA ATTTCTGGAA TGGGATTCAG
     3841 GAGTGGACAG AACGACACGG ATATATAGTG GATGTGTCAA AACGCATACC ATTTTGATAT
     3901 GAGATAATGC CGACTGTACT TTTTACAGTC GGTTTTCTAA TGTCACTAGG GCTCGCCTTT
     3961 GGGAAGTTTG AAGGGCTGGC ACGACAGGTT TCCCGACTGG AAAGCGGGCA GTGAGCGCAA
     4021 CGCAATTAAT GTGAGTTAGC TCACTCATTA GGCACCCCAG GCTTTACACT TTATGCTTCC
     4081 GGCTCGTATG TTGTGTGGAA TTGTGAGCGG ATAACAATTT CACACAGGAA ACAGCTATGA
     4141 CCATGATTAC GCCAAGCTTG CATGCCTGCA GGTCGACTCT AGAGGATCCC CGGGTACCGA
     4201 GCTCGAATTG ACAGATCGCT GAGATAGGTG CCTCACTGAT TAAGCATTGG TAACTGTCAG
     4261 ACCAAGTTTA CTCATATATA CTTTAGATTG ATTTAAAACT TCATTTTTAA TTTAAAAGGA
     4321 TCTAGGTGAA GATCCTTTTT GATAATCTCA TGACCAAAAT CCCTTAACGT GAGTTTTCGT
     4381 TCCACTGAGC GTCAGACCCC GTAGAAAAGA TCAAAGGATC TTC
//
