LOCUS       Exported                4216 bp DNA     circular SYN 21-JUL-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4216)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 21, 2021 from SnapGene 5.3.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4216
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     gene            23..958
                     /label=Rluc
                     /note="Rluc"
     misc_feature    993..1060
                     /label=HDV ribozyme short from p2RZ
                     /note="HDV ribozyme short from p2RZ"
     misc_feature    complement(1086..1105)
                     /label=SP6 promoter
                     /note="SP6 promoter"
     misc_feature    join(4200..4216,1..3)
                     /label=T7 promoter
                     /note="T7 promoter"
ORIGIN
        1 gggcgaattg gccaagtcgg ccatgacttc gaaagtttat gatccagaac aaaggaaacg
       61 gatgataact ggtccgcagt ggtgggccag atgtaaacaa atgaatgttc ttgattcatt
      121 tattaattat tatgattcag aaaaacatgc agaaaatgct gttatttttt tacatggtaa
      181 cgcggcctct tcttatttat ggcgacatgt tgtgccacat attgagccag tagcgcggtg
      241 tattatacca gaccttattg gtatgggcaa atcaggcaaa tctggtaatg gttcttatag
      301 gttacttgat cattacaaat atcttactgc atggtttgaa cttcttaatt taccaaagaa
      361 gatcattttt gtcggccatg attggggtgc ttgtttggca tttcattata gctatgagca
      421 tcaagataag atcaaagcaa tagttcacgc tgaaagtgta gtagatgtga ttgaatcatg
      481 ggatgaatgg cctgatattg aagaagatat tgcgttgatc aaatctgaag aaggagaaaa
      541 aatggttttg gagaataact tcttcgtgga aaccatgttg ccatcaaaaa tcatgagaaa
      601 gttagaacca gaagaatttg cagcatatct tgaaccattc aaagagaaag gtgaagttcg
      661 tcgtccaaca ttatcatggc ctcgtgaaat cccgttagta aaaggtggta aacctgacgt
      721 tgtacaaatt gttaggaatt ataatgctta tctacgtgca agtgatgatt taccaaaaat
      781 gtttattgaa tcggacccag gattcttttc caatgctatt gttgaaggtg ccaagaagtt
      841 tcctaatact gaatttgtca aagtaaaagg tcttcatttt tcgcaagaag atgcacctga
      901 tgaaatggga aaatatatca aatcgttcgt tgagcgagtt ctcaaaaatg aacaataacg
      961 aattcgtcga cctcgaggga tccgggccct ctgctagcca tggtcccagc ctcctcgctg
     1021 gcggctagtg ggcaacatgc ttcggcatgg cgaatgggac gcggccgcat gcataagctt
     1081 gagtattcta tagtgtcacc taaatagctt ggcgtaatca tggtcatagc tgtttcctgt
     1141 gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa
     1201 agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc
     1261 tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag
     1321 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
     1381 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
     1441 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
     1501 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
     1561 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
     1621 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
     1681 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
     1741 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
     1801 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
     1861 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
     1921 tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat
     1981 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
     2041 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
     2101 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
     2161 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
     2221 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
     2281 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
     2341 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg
     2401 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat
     2461 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat
     2521 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg
     2581 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc
     2641 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa
     2701 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc
     2761 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt
     2821 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag
     2881 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt
     2941 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag
     3001 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
     3061 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
     3121 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca
     3181 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg
     3241 ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca ttattatcat
     3301 gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtctcgcgc gtttcggtga
     3361 tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc
     3421 ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg
     3481 ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata tgcggtgtga
     3541 aataccgcac agatgcgtaa ggagaaaata ccgcatcagg aaattgtaag cgttaatatt
     3601 ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat tttttaacca ataggccgaa
     3661 atcggcaaaa tcccttataa atcaaaagaa tagaccgaga tagggttgag tgttgttcca
     3721 gtttggaaca agagtccact attaaagaac gtggactcca acgtcaaagg gcgaaaaacc
     3781 gtctatcagg gcgatggccc actacgtgaa ccatcaccct aatcaagttt tttggggtcg
     3841 aggtgccgta aagcactaaa tcggaaccct aaagggagcc cccgatttag agcttgacgg
     3901 ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg
     3961 gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg
     4021 ccgctacagg gcgcgtccat tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg
     4081 tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg atgtgctgca aggcgattaa
     4141 gttgggtaac gccagggttt tcccagtcac gacgttgtaa aacgacggcc agtgaattgt
     4201 aatacgactc actata
//
