LOCUS       Exported                4256 bp DNA     circular SYN 21-JUL-2021
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4256)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 21, 2021 from SnapGene 5.3.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4256
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     gene            23..958
                     /label=Rluc
                     /note="Rluc"
     misc_feature    1003..1032
                     /label=polyA
                     /note="polyA"
     misc_feature    1033..1100
                     /label=HDV ribozyme short from p2RZ
                     /note="HDV ribozyme short from p2RZ"
     misc_feature    complement(1126..1145)
                     /label=SP6 promoter
                     /note="SP6 promoter"
     misc_feature    join(4240..4256,1..3)
                     /label=T7 promoter
                     /note="T7 promoter"
ORIGIN
        1 gggcgaattg gccaagtcgg ccatgacttc gaaagtttat gatccagaac aaaggaaacg
       61 gatgataact ggtccgcagt ggtgggccag atgtaaacaa atgaatgttc ttgattcatt
      121 tattaattat tatgattcag aaaaacatgc agaaaatgct gttatttttt tacatggtaa
      181 cgcggcctct tcttatttat ggcgacatgt tgtgccacat attgagccag tagcgcggtg
      241 tattatacca gaccttattg gtatgggcaa atcaggcaaa tctggtaatg gttcttatag
      301 gttacttgat cattacaaat atcttactgc atggtttgaa cttcttaatt taccaaagaa
      361 gatcattttt gtcggccatg attggggtgc ttgtttggca tttcattata gctatgagca
      421 tcaagataag atcaaagcaa tagttcacgc tgaaagtgta gtagatgtga ttgaatcatg
      481 ggatgaatgg cctgatattg aagaagatat tgcgttgatc aaatctgaag aaggagaaaa
      541 aatggttttg gagaataact tcttcgtgga aaccatgttg ccatcaaaaa tcatgagaaa
      601 gttagaacca gaagaatttg cagcatatct tgaaccattc aaagagaaag gtgaagttcg
      661 tcgtccaaca ttatcatggc ctcgtgaaat cccgttagta aaaggtggta aacctgacgt
      721 tgtacaaatt gttaggaatt ataatgctta tctacgtgca agtgatgatt taccaaaaat
      781 gtttattgaa tcggacccag gattcttttc caatgctatt gttgaaggtg ccaagaagtt
      841 tcctaatact gaatttgtca aagtaaaagg tcttcatttt tcgcaagaag atgcacctga
      901 tgaaatggga aaatatatca aatcgttcgt tgagcgagtt ctcaaaaatg aacaataacg
      961 aattcgtcga cctcgaggga tccgggcgta gatccagaca tgaaaaaaaa aaaaaaaaaa
     1021 aaaaaaaaaa aagctagcca tggtcccagc ctcctcgctg gcggctagtg ggcaacatgc
     1081 ttcggcatgg cgaatgggac gcggccgcat gcataagctt gagtattcta tagtgtcacc
     1141 taaatagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca
     1201 caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag
     1261 tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt
     1321 cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc
     1381 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
     1441 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
     1501 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
     1561 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
     1621 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
     1681 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
     1741 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
     1801 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
     1861 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
     1921 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
     1981 ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag
     2041 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
     2101 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
     2161 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
     2221 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
     2281 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
     2341 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
     2401 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
     2461 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
     2521 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
     2581 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta
     2641 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
     2701 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
     2761 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
     2821 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
     2881 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
     2941 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
     3001 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
     3061 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
     3121 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
     3181 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
     3241 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
     3301 gaaaagtgcc acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata
     3361 ggcgtatcac gaggcccttt cgtctcgcgc gtttcggtga tgacggtgaa aacctctgac
     3421 acatgcagct cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag
     3481 cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat
     3541 cagagcagat tgtactgaga gtgcaccata tgcggtgtga aataccgcac agatgcgtaa
     3601 ggagaaaata ccgcatcagg aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa
     3661 tttttgttaa atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa
     3721 atcaaaagaa tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact
     3781 attaaagaac gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc
     3841 actacgtgaa ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa
     3901 tcggaaccct aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc
     3961 gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt
     4021 cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtccat
     4081 tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta
     4141 cgccagctgg cgaaaggggg atgtgctgca aggcgattaa gttgggtaac gccagggttt
     4201 tcccagtcac gacgttgtaa aacgacggcc agtgaattgt aatacgactc actata
//
