LOCUS       Exported                4020 bp DNA     circular SYN 21-JUL-2021
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4020)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 21, 2021 from SnapGene 5.3.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4020
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    4..69
                     /label=X. laevis beta globin 5'UTR
                     /note="X. laevis beta globin 5'UTR"
     CDS             70..585
                     /codon_start=1
                     /label=NanoLuc
                     /note="NanoLuc"
                     /translation="MVFTLEDFVGDWRQTAGYNLDQVLEQGGVSSLFQNLGVSVTPIQR
                     IVLSGENGLKIDIHVIIPYEGLSGDQMGQIEKIFKVVYPVDDHHFKVILHYGTLVIDGV
                     TPNMIDYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLINPDGSLLFRVTINGVTGW
                     RLCERILA"
     misc_feature    586..715
                     /label=X. laevis beta globin 3'-UTR
                     /note="X. laevis beta globin 3'-UTR"
     misc_feature    734..796
                     /label=polyA
                     /note="polyA"
     misc_feature    797..864
                     /label=HDV ribozyme short from p2RZ
                     /note="HDV ribozyme short from p2RZ"
     misc_feature    complement(890..909)
                     /label=SP6 promoter
                     /note="SP6 promoter"
     misc_feature    join(4004..4020,1..3)
                     /label=T7 promoter
                     /note="T7 promoter"
ORIGIN
        1 ggggaataca agcttcttgt tctttttgca gaagctcaga ataaacgctc aactttggca
       61 gatctgaaca tggtcttcac actcgaagat ttcgttgggg actggcgaca gacagccggc
      121 tacaacctgg accaagtcct tgaacaggga ggtgtgtcca gtttgtttca gaatctcggg
      181 gtgtccgtaa ctccgatcca aaggattgtc ctgagcggtg aaaatgggct gaagatcgac
      241 atccatgtca tcatcccgta tgaaggtctg agcggcgacc aaatgggcca gatcgaaaaa
      301 atttttaagg tggtgtaccc tgtggatgat catcacttta aggtgatcct gcactatggc
      361 acactggtaa tcgacggggt tacgccgaac atgatcgact atttcggacg gccgtatgaa
      421 ggcatcgccg tgttcgacgg caaaaagatc actgtaacag ggaccctgtg gaacggcaac
      481 aaaattatcg acgagcgcct gatcaacccc gacggctccc tgctgttccg agtaaccatc
      541 aacggagtga ccggctggcg gctgtgcgaa cgcattctgg cgtaaaccag cctcaagaac
      601 acccgaatgg agtctctaag ctacataata ccaacttaca ctttacaaaa tgttgtcccc
      661 caaaatgtag ccattcgtat ctgctcctaa taaaaagaaa gtttcttcac attctggcgt
      721 agatccagac atgaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
      781 aaaaaaaaaa aaaaaagcta gccatggtcc cagcctcctc gctggcggct agtgggcaac
      841 atgcttcggc atggcgaatg ggacgcggcc gcatgcataa gcttgagtat tctatagtgt
      901 cacctaaata gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg
      961 ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa
     1021 tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac
     1081 ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt
     1141 gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
     1201 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
     1261 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
     1321 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
     1381 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
     1441 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
     1501 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
     1561 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
     1621 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
     1681 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
     1741 tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag
     1801 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
     1861 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
     1921 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
     1981 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
     2041 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
     2101 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
     2161 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
     2221 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga
     2281 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
     2341 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
     2401 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
     2461 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
     2521 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
     2581 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
     2641 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
     2701 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
     2761 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
     2821 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
     2881 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
     2941 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
     3001 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
     3061 ccccgaaaag tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa
     3121 aataggcgta tcacgaggcc ctttcgtctc gcgcgtttcg gtgatgacgg tgaaaacctc
     3181 tgacacatgc agctcccgga gacggtcaca gcttgtctgt aagcggatgc cgggagcaga
     3241 caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggctggct taactatgcg
     3301 gcatcagagc agattgtact gagagtgcac catatgcggt gtgaaatacc gcacagatgc
     3361 gtaaggagaa aataccgcat caggaaattg taagcgttaa tattttgtta aaattcgcgt
     3421 taaatttttg ttaaatcagc tcatttttta accaataggc cgaaatcggc aaaatccctt
     3481 ataaatcaaa agaatagacc gagatagggt tgagtgttgt tccagtttgg aacaagagtc
     3541 cactattaaa gaacgtggac tccaacgtca aagggcgaaa aaccgtctat cagggcgatg
     3601 gcccactacg tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac
     3661 taaatcggaa ccctaaaggg agcccccgat ttagagcttg acggggaaag ccggcgaacg
     3721 tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag
     3781 cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt
     3841 ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct
     3901 attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg
     3961 gttttcccag tcacgacgtt gtaaaacgac ggccagtgaa ttgtaatacg actcactata
//
