LOCUS       Exported                5570 bp DNA     circular SYN 22-JUL-2021
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5570)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 22, 2021 from SnapGene 5.3.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5570
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    4..69
                     /label=X. laevis beta globin 5'UTR
                     /note="X. laevis beta globin 5'UTR"
     CDS             70..1968
                     /codon_start=1
                     /label=X.L. PABPC1
                     /translation="MNPSAPSYPMASLYVGDLHQDVTEAMLYEKFSPAGPILSIRVCRD
                     MITRRSLGYAYVNFQQPADAERALDTMNFDVIKGRPVRIMWSQRDPSLRKSGVGNIFIK
                     NLDKSIDNKALYDTFSAFGNILSCKVVCDENGSKGYGFVHFETQEAAERAIDKMNGMLL
                     NDRKVFVGRFKSRKEREAELGARAKEFTNVYIKNFGDDMNDERLKEMFGKYGPALSVKV
                     MTDDNGKSKGFGFVSFERHEDAQKAVDEMYGKDMNGKSMFVGRAQKKVERQTELKRKFE
                     QMNQDRITRYQGVNLYVKNLDDGIDDERLRKEFLPFGTITSAKVMMEGGRSKGFGFVCF
                     SSPEEATKAVTEMNGRIVATKPLYVALAQRKEERQAHLTNQYMQRMASVRVPNPVINPY
                     QPPPSSYFMAAIPPAQNRAAYYPPGQIAQLRPSPRWTAQGARPHPFQNMPGAIRPTAPR
                     PPTFSTMRPASNQVPRVMSAQRVANTSTQTMGPRPTTAAAAAASAVRAVPQYKYAAGVR
                     NQQHLNTQPQVAMQQPAVHVQGQEPLTASMLAAAPPQEQKQMLGERLFPLIQAMHPTLA
                     GKITGMLLEIDNSELLHMLESPESLRLKVDEAVAVLQAHQAKEAAQKVVNATGVPTA"
     misc_feature    1969..1992
                     /label=Flag Tag
                     /note="Flag Tag"
     misc_feature    1996..2125
                     /label=X. laevis beta globin 3'-UTR
                     /note="X. laevis beta globin 3'-UTR"
     misc_feature    2144..2243
                     /label=polyA
                     /note="polyA"
     misc_feature    2244..2346
                     /label=mouse MALAT1 6588-6690
                     /note="mouse MALAT1 6588-6690"
     misc_feature    2347..2414
                     /label=HDV ribozyme short from p2RZ
                     /note="HDV ribozyme short from p2RZ"
     misc_feature    complement(2440..2459)
                     /label=SP6 promoter
                     /note="SP6 promoter"
     misc_feature    join(5554..5570,1..3)
                     /label=T7 promoter
                     /note="T7 promoter"
ORIGIN
        1 ggggaataca agcttcttgt tctttttgca gaagctcaga ataaacgctc aactttggca
       61 gatctgaaca tgaatcccag tgctcccagc tacccaatgg cttccctgta cgttggagac
      121 cttcatcaag atgtaacgga ggccatgctt tatgagaagt ttagcccagc tggtcccatt
      181 ttgtcaatcc gagtttgcag agatatgatt acacgacgct cacttggata tgcatatgtc
      241 aacttccagc aaccagctga tgctgaacgt gctttggata caatgaactt tgatgtcata
      301 aagggcaggc ccgtgcggat tatgtggtct cagcgtgacc cttctctgcg taagagtgga
      361 gttggtaaca ttttcatcaa aaacctggac aagtctattg ataacaaagc cttgtacgat
      421 acattttctg catttggaaa cattctttcc tgtaaggtgg tttgtgatga aaatggatcc
      481 aagggctatg gttttgttca ctttgagaca caagaggctg ctgagagggc tattgataaa
      541 atgaatggca tgcttctcaa tgaccgcaaa gtatttgttg ggcgctttaa gtcccgcaaa
      601 gagcgtgaag ctgagcttgg tgccagagct aaggaattta caaatgttta catcaaaaat
      661 tttggagacg acatgaatga cgagaggctc aaagaaatgt ttggcaaata tgggccagct
      721 cttagtgtta aagttatgac tgatgacaat ggaaagtcaa aaggctttgg ctttgtcagc
      781 tttgaaagac atgaagatgc acaaaaggct gttgatgaaa tgtatggcaa ggatatgaat
      841 gggaagtcca tgtttgtggg ccgtgcacag aaaaaggtgg aaaggcaaac tgagcttaag
      901 cgcaagtttg aacaaatgaa tcaggaccga atcaccagat accagggtgt taacctctat
      961 gttaaaaacc ttgatgatgg tattgatgat gaacgactgc ggaaagaatt cttacctttt
     1021 ggtacaatca ccagtgctaa ggtaatgatg gaaggtggtc gcagtaaagg ctttggtttt
     1081 gtatgctttt cttcacctga agaggccact aaagcagtca cagaaatgaa tggtagaatt
     1141 gttgccacaa aacccctgta tgttgcattg gcccaaagaa aagaagagcg tcaggctcac
     1201 ttgaccaacc agtacatgca gaggatggca agtgttcgtg taccaaatcc tgtgatcaac
     1261 ccataccagc caccaccatc cagctatttc atggcagcta tcccaccggc tcaaaaccgt
     1321 gctgcgtact acccacctgg gcagattgca cagctcaggc ccagcccccg ttggactgca
     1381 cagggtgcca gacctcatcc attccagaac atgcccggag caatccgccc tactgctcca
     1441 agaccaccaa cattcagtac aatgagaccg gcttctaatc aagtcccgcg tgtcatgtca
     1501 gctcaacgtg ttgccaatac atcaacccag actatgggtc ctcgtccaac gactgctgct
     1561 gcagctgcag catctgctgt cagggctgtt cctcagtata aatatgcggc gggtgtacgt
     1621 aatcagcagc atcttaatac acagccccaa gtggctatgc agcagcctgc tgtccatgta
     1681 caaggccagg aacctctgac ggcttccatg ctggcagctg ctccacctca agagcagaaa
     1741 caaatgctag gtgagcggct cttcccactt atccaagcaa tgcacccaac tctggctggc
     1801 aaaattactg gaatgcttct ggagattgac aactcggagc ttctccatat gcttgaatca
     1861 cccgagtcac tgcgcttaaa ggttgatgaa gctgttgctg tgcttcaagc ccatcaggct
     1921 aaggaagctg ctcagaaggt tgttaatgca actggagtgc caactgctga ttacaaggat
     1981 gacgacgata agtaaaccag cctcaagaac acccgaatgg agtctctaag ctacataata
     2041 ccaacttaca ctttacaaaa tgttgtcccc caaaatgtag ccattcgtat ctgctcctaa
     2101 taaaaagaaa gtttcttcac attctggcgt agatccagac atgaaaaaaa aaaaaaaaaa
     2161 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
     2221 aaaaaaaaaa aaaaaaaaaa aaacagtagg gttgtaaagg tttttctttt cctgagaaaa
     2281 caaccttttg ttttctcagg ttttgctttt tggcctttcc ctagctttaa aaaaaaaaaa
     2341 gcaaaagcta gccatggtcc cagcctcctc gctggcggct agtgggcaac atgcttcggc
     2401 atggcgaatg ggacgcggcc gcatgcataa gcttgagtat tctatagtgt cacctaaata
     2461 gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc
     2521 cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct
     2581 aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc
     2641 agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt
     2701 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
     2761 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
     2821 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
     2881 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
     2941 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
     3001 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
     3061 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
     3121 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
     3181 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
     3241 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
     3301 actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct
     3361 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
     3421 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
     3481 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
     3541 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
     3601 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
     3661 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
     3721 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
     3781 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
     3841 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
     3901 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca
     3961 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
     4021 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
     4081 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
     4141 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
     4201 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg
     4261 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
     4321 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
     4381 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
     4441 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
     4501 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
     4561 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag
     4621 tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta
     4681 tcacgaggcc ctttcgtctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc
     4741 agctcccgga gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc
     4801 agggcgcgtc agcgggtgtt ggcgggtgtc ggggctggct taactatgcg gcatcagagc
     4861 agattgtact gagagtgcac catatgcggt gtgaaatacc gcacagatgc gtaaggagaa
     4921 aataccgcat caggaaattg taagcgttaa tattttgtta aaattcgcgt taaatttttg
     4981 ttaaatcagc tcatttttta accaataggc cgaaatcggc aaaatccctt ataaatcaaa
     5041 agaatagacc gagatagggt tgagtgttgt tccagtttgg aacaagagtc cactattaaa
     5101 gaacgtggac tccaacgtca aagggcgaaa aaccgtctat cagggcgatg gcccactacg
     5161 tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa
     5221 ccctaaaggg agcccccgat ttagagcttg acggggaaag ccggcgaacg tggcgagaaa
     5281 ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct
     5341 gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt ccattcgcca
     5401 ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct attacgccag
     5461 ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg gttttcccag
     5521 tcacgacgtt gtaaaacgac ggccagtgaa ttgtaatacg actcactata
//
