LOCUS       Exported                5558 bp DNA     circular SYN 22-JUL-2021
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5558)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 22, 2021 from SnapGene 5.3.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5558
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    4..69
                     /label=X. laevis beta globin 5'UTR
                     /note="X. laevis beta globin 5'UTR"
     CDS             70..1956
                     /codon_start=1
                     /label=ePAB
                     /note="ePAB"
                     /translation="MNATRAEYPLASLYIGDLHPDVTEAMLYEKFSPAGPIMSIRVCRD
                     IATRRSLGYAYINFQQPADAERALDTMNFEVIKGRPIRIMWSQRDPGLRKSGVGNVFIK
                     NLDDSIDNKALYDTFSAFGDILSCKVVCDEYGSRGYGFVHFETQEAANRAIQTMNGMLL
                     NDRKVFVGHFKSRRERELEYGAKVMEFTNVYIKNFGEDMDDKRLKEIFSAFGNTLSVKV
                     MMDNSGRSRGFGFVNYGNHEEAQKAVTEMNGKEVNGRMVYVGRAQKRIERQGELKRKFE
                     QIKQERINRYQGVNLYVKNLDDGIDDDRLRKEFSPYGTITSTKVMTEGGHSKGFGFVCF
                     SSPEEATKAVTEMNGRIVSTKPLYVALAQRKEERKAILTNQYMQRLATMRAMPGPLLGS
                     FQQPANYFLPTMPQPSNRAFYSPNPVAPVRPAPQWASHQSRPPQYQPPAPLMRAVPPRR
                     MHSNISTMKQASTQVPRVPLQSQRVANIGTQTAGARAQVNASIMRAMPHYKYSCGVRNV
                     QPIGSSAHLQQVLEPAVLMQGQEPLTASLLAAAPLQEQKQILGERIYPLIHEMHPTLAG
                     KITGMLLEIDNSELLHMLESPESLHSKVEEAVAVLQAHQAKESAPKSAPQSLI"
     misc_feature    1957..1980
                     /label=Flag Tag
                     /note="Flag Tag"
     misc_feature    1984..2113
                     /label=X. laevis beta globin 3'-UTR
                     /note="X. laevis beta globin 3'-UTR"
     misc_feature    2132..2231
                     /label=polyA
                     /note="polyA"
     misc_feature    2232..2334
                     /label=mouse MALAT1 6588-6690
                     /note="mouse MALAT1 6588-6690"
     misc_feature    2335..2402
                     /label=HDV ribozyme short from p2RZ
                     /note="HDV ribozyme short from p2RZ"
     misc_feature    complement(2428..2447)
                     /label=SP6 promoter
                     /note="SP6 promoter"
     misc_feature    join(5542..5558,1..3)
                     /label=T7 promoter
                     /note="T7 promoter"
ORIGIN
        1 ggggaataca agcttcttgt tctttttgca gaagctcaga ataaacgctc aactttggca
       61 gatctgaaca tgaacgcaac cagagccgaa tacccgctgg catctcttta tataggggac
      121 ctccaccccg acgtgacgga agcgatgctg tatgagaagt tttctccagc ggggcccatc
      181 atgtctataa gagtgtgtcg agatattgct actcgccggt cccttggcta tgcctacatt
      241 aatttccagc agccggctga tgctgagcgg gccttggata caatgaattt tgaggttatt
      301 aaaggcaggc caatccgaat catgtggtcc cagagagatc cgggccttcg aaaatcgggt
      361 gtagggaatg tatttattaa aaatttagac gactccatcg acaacaaagc cctgtatgac
      421 actttctcag cgtttggaga tattttatct tgtaaggtgg tttgcgatga atacggatct
      481 cgaggttatg gtttcgtaca ttttgagacc caagaagctg ccaatcgagc gattcagact
      541 atgaatggga tgttgctcaa cgaccgcaaa gtgtttgtgg gccattttaa atcacggagg
      601 gaaagagaat tagaatatgg ggcaaaagtc atggaattta ccaacgtgta catcaagaac
      661 ttcggggagg atatggacga taaaagactt aaagaaatat tttctgcctt tggaaatacg
      721 ttaagcgtta aagtaatgat ggataatagt ggtcgatccc ggggttttgg ttttgttaat
      781 tatggaaatc acgaagaagc acaaaaagca gttactgaga tgaacggtaa agaagtaaac
      841 ggtcgaatgg tttacgtagg cagagctcag aagaggatag aacggcaggg cgagttaaaa
      901 cgcaagttcg aacaaatcaa gcaagaacga atcaaccgct atcagggtgt taatttgtat
      961 gttaaaaacc tggacgatgg tatcgatgat gaccggctca gaaaggagtt ctcgccgtat
     1021 ggtaccatta ccagtacaaa ggtaatgact gaaggaggcc atagtaaagg ctttggtttt
     1081 gtttgcttct cctccccaga agaagccact aaagcagtca cagaaatgaa cgggaggata
     1141 gtcagtacga agcctctata tgttgcactt gcacaaagga aggaagaacg gaaggcaatt
     1201 cttaccaatc agtacatgca gagactagcc actatgaggg cgatgcctgg tcctctcctt
     1261 gggtccttcc agcaacctgc taattatttc ttgcctacaa tgccacagcc ttcaaacaga
     1321 gcattttata gtccaaatcc tgttgctcct gttcgtccag ctcctcagtg ggcttcccac
     1381 cagtccaggc ctccacagta ccaaccacca gccccattaa tgagggcggt gccaccaaga
     1441 aggatgcatt caaatatcag tacaatgaag caggcatcaa cacaagtacc cagggtgcct
     1501 ctgcagagtc aaagagtagc aaacattggc actcaaacag ctggagctcg ggcccaagta
     1561 aacgcgtcca taatgcgcgc aatgccacat tacaagtatt cctgtggcgt gaggaacgtg
     1621 cagcctatag gaagctctgc tcatctgcaa caggtactgg aacctgcagt tctgatgcaa
     1681 ggacaagaac cgctaactgc atctctgctt gcagctgccc cactgcagga acagaaacaa
     1741 atactcgggg aacgtattta cccgttaata catgaaatgc atccgacgtt agccggaaag
     1801 ataacgggaa tgctgctgga aattgataac tctgagctac tgcatatgct ggagtcacca
     1861 gagtctctgc attcaaaggt ggaagaggca gtggcggtcc tgcaagccca ccaagccaag
     1921 gaaagtgctc cgaaaagtgc tccgcagtct ctgatcgatt acaaggatga cgacgataag
     1981 taaaccagcc tcaagaacac ccgaatggag tctctaagct acataatacc aacttacact
     2041 ttacaaaatg ttgtccccca aaatgtagcc attcgtatct gctcctaata aaaagaaagt
     2101 ttcttcacat tctggcgtag atccagacat gaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
     2161 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
     2221 aaaaaaaaaa acagtagggt tgtaaaggtt tttcttttcc tgagaaaaca accttttgtt
     2281 ttctcaggtt ttgctttttg gcctttccct agctttaaaa aaaaaaaagc aaaagctagc
     2341 catggtccca gcctcctcgc tggcggctag tgggcaacat gcttcggcat ggcgaatggg
     2401 acgcggccgc atgcataagc ttgagtattc tatagtgtca cctaaatagc ttggcgtaat
     2461 catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac
     2521 gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa
     2581 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat
     2641 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc
     2701 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
     2761 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
     2821 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
     2881 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     2941 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     3001 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     3061 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     3121 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     3181 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
     3241 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
     3301 ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
     3361 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
     3421 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
     3481 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
     3541 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
     3601 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
     3661 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
     3721 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
     3781 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
     3841 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
     3901 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac
     3961 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
     4021 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
     4081 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
     4141 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
     4201 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc
     4261 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
     4321 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
     4381 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
     4441 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
     4501 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
     4561 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg
     4621 tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct
     4681 ttcgtctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga
     4741 cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag
     4801 cgggtgttgg cgggtgtcgg ggctggctta actatgcggc atcagagcag attgtactga
     4861 gagtgcacca tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca
     4921 ggaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
     4981 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
     5041 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
     5101 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
     5161 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
     5221 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
     5281 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
     5341 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc attcgccatt caggctgcgc
     5401 aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg
     5461 ggatgtgctg caaggcgatt aagttgggta acgccagggt tttcccagtc acgacgttgt
     5521 aaaacgacgg ccagtgaatt gtaatacgac tcactata
//
