LOCUS       Exported                8489 bp DNA     circular SYN 22-JUL-2021
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8489)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 22, 2021 from SnapGene 5.3.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8489
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    4..69
                     /label=X. laevis beta globin 5'UTR
                     /note="X. laevis beta globin 5'UTR"
     CDS             70..4887
                     /codon_start=1
                     /label=H.S. eIF4G-1
                     /note="NM_001194946.1"
                     /translation="MNKAPQSTGPPPAPSPGLPQPAFPPGQTAPVVFSTPQATQMNTPS
                     QPRQGGFRSLQHFYPSRAQPPSSAASRVQSAAPARPGPAAHVYPAGSQVMMIPSQISYP
                     ASQGAYYIPGQGRSTYVVPTQQYPVQPGAPGFYPGASPTEFGTYAGAYYPAQGVQQFPT
                     GVAPAPVLMNQPPQIAPKRERKTIRIRDPNQGGKDITEEIMSGARTASTPTPPQTGGGL
                     EPQANGETPQVAVIVRPDDRSQGAIIADRPGLPGPEHSPSESQPSSPSPTPSPSPVLEP
                     GSEPNLAVLSIPGDTMTTIQMSVEESTPISRETGEPYRLSPEPTPLAEPILEVEVTLSK
                     PVPESEFSSSPLQAPTPLASHTVEIHEPNGMVPSEDLEPEVESSPELAPPPACPSESPV
                     PIAPTAQPEELLNGAPSPPAVDLSPVSEPEEQAKEVTASVAPPTIPSATPATAPSATSP
                     AQEEEMEEEEEEEEGEAGEAGEAESEKGGEELLPPESTPIPANLSQNLEAAAATQVAVS
                     VPKRRRKIKELNKKEAVGDLLDAFKEANPAVPEVENQPPAGSNPGPESEGSGVPPRPEE
                     ADETWDSKEDKIHNAENIQPGEQKYEYKSDQWKPLNLEEKKRYDREFLLGFQFIFASMQ
                     KPEGLPHISDVVLDKANKTPLRPLDPTRLQGINCGPDFTPSFANLGRTTLSTRGPPRGG
                     PGGELPRGPAGLGPRRSQQGPRKEPRKIIATVLMTEDIKLNKAEKAWKPSSKRTAADKD
                     RGEEDADGSKTQDLFRRVRSILNKLTPQMFQQLMKQVTQLAIDTEERLKGVIDLIFEKA
                     ISEPNFSVAYANMCRCLMALKVPTTEKPTVTVNFRKLLLNRCQKEFEKDKDDDEVFEKK
                     QKEMDEAATAEERGRLKEELEEARDIARRRSLGNIKFIGELFKLKMLTEAIMHDCVVKL
                     LKNHDEESLECLCRLLTTIGKDLDFEKAKPRMDQYFNQMEKIIKEKKTSSRIRFMLQDV
                     LDLRGSNWVPRRGDQGPKTIDQIHKEAEMEEHREHIKVQQLMAKGSDKRRGGPPGPPIS
                     RGLPLVDDGGWNTVPISKGSRPIDTSRLTKITKPGSIDSNNQLFAPGGRLSWGKGSSGG
                     SGAKPSDAASEAARPATSTLNRFSALQQAVPTESTDNRRVVQRSSLSRERGEKAGDRGD
                     RLERSERGGDRGDRLDRARTPATKRSFSKEVEERSRERPSQPEGLRKAASLTEDRDRGR
                     DAVKREAALPPVSPLKAALSEEELEKKSKAIIEEYLHLNDMKEAVQCVQELASPSLLFI
                     FVRHGVESTLERSAIAREHMGQLLHQLLCAGHLSTAQYYQGLYEILELAEDMEIDIPHV
                     WLYLAELVTPILQEGGVPMGELFREITKPLRPLGKAASLLLEILGLLCKSMGPKKVGTL
                     WREAGLSWKEFLPEGQDIGAFVAEQKVEYTLGEESEAPGQRALPSEELNRQLEKLLKEG
                     SSNQRVFDWIEANLSEQQIVSNTLVRALMTAVCYSAIIFETPLRVDVAVLKARAKLLQK
                     YLCDEQKELQALYALQALVVTLEQPPNLLRMFFDALYDEDVVKEDAFYSWESSKDPAEQ
                     QGKGVALKSVTAFFKWLREAEEESDHN"
     misc_feature    4888..4911
                     /label=Flag Tag
                     /note="Flag Tag"
     misc_feature    4915..5044
                     /label=X. laevis beta globin 3'-UTR
                     /note="X. laevis beta globin 3'-UTR"
     misc_feature    5063..5162
                     /label=polyA
                     /note="polyA"
     misc_feature    5163..5265
                     /label=mouse MALAT1 6588-6690
                     /note="mouse MALAT1 6588-6690"
     misc_feature    5266..5333
                     /label=HDV ribozyme short from p2RZ
                     /note="HDV ribozyme short from p2RZ"
     misc_feature    complement(5359..5378)
                     /label=SP6 promoter
                     /note="SP6 promoter"
     misc_feature    join(8473..8489,1..3)
                     /label=T7 promoter
                     /note="T7 promoter"
ORIGIN
        1 ggggaataca agcttcttgt tctttttgca gaagctcaga ataaacgctc aactttggca
       61 gatctgaaca tgaacaaagc tccacagtcc acaggccccc cacccgcccc atcccccgga
      121 ctcccacagc cagcgtttcc cccggggcag acagcgccgg tggtgttcag tacgccacaa
      181 gcgacacaaa tgaacacgcc ttctcagccc cgccagggag gattcaggtc tctgcagcac
      241 ttctacccta gccgggccca gcccccgagc agtgcagcct cccgagtgca gagtgcagcc
      301 cctgcccgcc ctggcccagc tgcccatgtc taccctgctg gatcccaagt aatgatgatc
      361 ccttcccaga tctcctaccc agcctcccag ggggcctact acatccctgg acaggggcgt
      421 tccacatacg ttgtcccgac acagcagtac cctgtgcagc caggagcccc aggcttctat
      481 ccaggtgcaa gccctacaga atttgggacc tacgctggcg cctactatcc agcccaaggg
      541 gtgcagcagt ttcccactgg cgtggccccc gccccagttt tgatgaacca gccaccccag
      601 attgctccca agagggagcg taagacgatc cgaattcgag atccaaacca aggaggaaag
      661 gatatcacag aggagatcat gtctggggcc cgcactgcct ccacacccac ccctccccag
      721 acgggaggcg gtctggagcc tcaagctaat ggggagacgc cccaggttgc tgtcattgtc
      781 cggccagatg accggtcaca gggagcaatc attgctgacc ggccagggct gcctggccca
      841 gagcatagcc cttcagaatc ccagccttcg tcgccttctc cgaccccatc accatcccca
      901 gtcttggaac cggggtctga gcctaatctc gcagtcctct ctattcctgg ggacactatg
      961 acaactatac aaatgtctgt agaagaatca acccccatct cccgtgaaac tggggagcca
     1021 tatcgcctct ctccagaacc cactcctctc gccgaaccca tactggaagt agaagtgaca
     1081 cttagcaaac cggttccaga atctgagttt tcttccagtc ctctccaggc tcccacccct
     1141 ttggcatctc acacagtgga aattcatgag cctaatggca tggtcccatc tgaagatctg
     1201 gaaccagagg tggagtcaag cccagagctt gctcctcccc cagcttgccc ctccgaatcc
     1261 cctgtgccca ttgctccaac tgcccaacct gaggaactgc tcaacggagc cccctcgcca
     1321 ccagctgtgg acttaagccc agtcagtgag ccagaggagc aggccaagga ggtgacagca
     1381 tcagtggcgc cccccaccat cccctctgct actccagcta cggctccttc agctacttcc
     1441 ccagctcagg aggaggaaat ggaagaagaa gaagaagagg aagaaggaga agcaggagaa
     1501 gcaggagaag ctgagagtga gaaaggagga gaggaactgc tccccccaga gagtacccct
     1561 attccagcca acttgtctca gaatttggag gcagcagcag ccactcaagt ggcagtatct
     1621 gtgccaaaga ggagacggaa aattaaggag ctaaataaga aggaggctgt tggagacctt
     1681 ctggatgcct tcaaggaggc gaacccggca gtaccagagg tggaaaatca gcctcctgca
     1741 ggcagcaatc caggcccaga gtctgagggc agtggtgtgc ccccacgtcc tgaggaagca
     1801 gatgagacct gggactcaaa ggaagacaaa attcacaatg ctgagaacat ccagcccggg
     1861 gaacagaagt atgaatataa gtcagatcag tggaagcctc taaacctaga ggagaaaaaa
     1921 cgttacgacc gtgagttcct gcttggtttt cagttcatct ttgccagtat gcagaagcca
     1981 gagggattgc cacatatcag tgacgtggtg ctggacaagg ccaataaaac accactgcgg
     2041 ccactggatc ccactagact acaaggcata aattgtggcc cagacttcac tccatccttt
     2101 gccaaccttg gccggacaac ccttagcacc cgtgggcccc caaggggtgg gccaggtggg
     2161 gagctgcccc gtgggccggc tggcctggga ccccggcgct ctcagcaggg accccgaaaa
     2221 gaaccacgca agatcattgc cacagtgtta atgaccgaag atataaaact gaacaaagca
     2281 gagaaagcct ggaaacccag cagcaagcgg acggcggctg ataaggatcg aggggaagaa
     2341 gatgctgatg gcagcaaaac ccaggaccta ttccgcaggg tgcgctccat cctgaataaa
     2401 ctgacacccc agatgttcca gcagctgatg aagcaagtga cgcagctggc catcgacacc
     2461 gaggaacgcc tcaaaggggt cattgacctc atttttgaga aggccatttc agagcccaac
     2521 ttctctgtgg cctatgccaa catgtgccgc tgcctcatgg cgctgaaagt gcccactacg
     2581 gaaaagccaa cagtgactgt gaacttccga aagctgttgt tgaatcgatg tcagaaggag
     2641 tttgagaaag acaaagatga tgatgaggtt tttgagaaga agcaaaaaga gatggatgaa
     2701 gctgctacgg cagaggaacg aggacgcctg aaggaagagc tggaagaggc tcgggacata
     2761 gcccggcggc gctctttagg gaatatcaag tttattggag agttgttcaa actgaagatg
     2821 ttaacagagg caataatgca tgactgtgtg gtcaaactgc ttaagaacca tgatgaagag
     2881 tcccttgagt gcctttgtcg tctgctcacc accattggca aagacctgga ctttgaaaaa
     2941 gccaagcccc gaatggatca gtatttcaac cagatggaaa aaatcattaa agaaaagaag
     3001 acgtcatccc gcatccgctt tatgctgcag gacgtgctgg atctgcgagg gagcaattgg
     3061 gtgccacgcc gaggggatca gggtcccaag accattgacc agatccataa ggaggctgag
     3121 atggaagaac atcgagagca catcaaagtg cagcagctca tggccaaggg cagtgacaag
     3181 cgtcggggcg gtcctccagg ccctcccatc agccgtggac ttccccttgt ggatgatggt
     3241 ggctggaaca cagttcccat cagcaaaggt agccgcccca ttgacacctc acgactcacc
     3301 aagatcacca agcctggctc catcgattct aacaaccagc tctttgcacc tggagggcga
     3361 ctgagctggg gcaagggcag cagcggaggc tcaggagcca agccctcaga cgcagcatca
     3421 gaagctgctc gcccagctac tagtactttg aatcgcttct cagcccttca acaagcggta
     3481 cccacagaaa gcacagataa tagacgtgtg gtgcagagga gtagcttgag ccgagaacga
     3541 ggcgagaaag ctggagaccg aggagaccgc ctagagcgga gtgaacgggg aggggaccgt
     3601 ggggaccggc ttgatcgtgc gcggacacct gctaccaagc ggagcttcag caaggaagtg
     3661 gaggagcgga gtagagaacg gccctcccag cctgaggggc tgcgcaaggc agctagcctc
     3721 acggaggatc gggaccgtgg gcgggatgcc gtgaagcgag aagctgccct acccccagtg
     3781 agccccctga aggcggctct ctctgaggag gagttagaga agaaatccaa ggctatcatt
     3841 gaggaatatc tccatctcaa tgacatgaaa gaggcagtcc agtgcgtgca ggagctggcc
     3901 tcaccctcct tgctcttcat ctttgtacgg catggtgtcg agtctacgct ggagcgcagt
     3961 gccattgctc gtgagcatat ggggcagctg ctgcaccagc tgctctgtgc tgggcatctg
     4021 tctactgctc agtactacca agggttgtat gaaatcttgg aattggctga ggacatggaa
     4081 attgacatcc cccatgtgtg gctctaccta gcggaactgg taacacccat tctgcaggaa
     4141 ggtggggtgc ccatggggga gctgttcagg gagattacaa agcctctgag accgttgggc
     4201 aaagctgctt ccctgttgct ggagatcctg ggcctcctgt gcaaaagcat gggtcctaaa
     4261 aaggtgggga cgctgtggcg agaagccggg cttagctgga aggaatttct acctgaaggc
     4321 caggacattg gtgcattcgt cgctgaacag aaggtggagt ataccctggg agaggagtcg
     4381 gaagcccctg gccagagggc actcccctcc gaggagctga acaggcagct ggagaagctg
     4441 ctgaaggagg gcagcagtaa ccagcgggtg ttcgactgga tagaggccaa cctgagtgag
     4501 cagcagatag tatccaacac gttagttcga gccctcatga cggctgtctg ctattctgca
     4561 attatttttg agactcccct ccgagtggac gttgcagtgc tgaaagcgcg agcgaagctg
     4621 ctgcagaaat acctgtgtga cgagcagaag gagctacagg cgctctacgc cctccaggcc
     4681 cttgtagtga ccttagaaca gcctcccaac ctgctgcgga tgttctttga cgcactgtat
     4741 gacgaggacg tggtgaagga ggatgccttc tacagttggg agagtagcaa ggaccccgct
     4801 gagcagcagg gcaagggtgt ggcccttaaa tctgtcacag ccttcttcaa gtggctccgt
     4861 gaagcagagg aggagtctga ccacaacgat tacaaggatg acgacgataa gtaaaccagc
     4921 ctcaagaaca cccgaatgga gtctctaagc tacataatac caacttacac tttacaaaat
     4981 gttgtccccc aaaatgtagc cattcgtatc tgctcctaat aaaaagaaag tttcttcaca
     5041 ttctggcgta gatccagaca tgaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
     5101 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
     5161 aacagtaggg ttgtaaaggt ttttcttttc ctgagaaaac aaccttttgt tttctcaggt
     5221 tttgcttttt ggcctttccc tagctttaaa aaaaaaaaag caaaagctag ccatggtccc
     5281 agcctcctcg ctggcggcta gtgggcaaca tgcttcggca tggcgaatgg gacgcggccg
     5341 catgcataag cttgagtatt ctatagtgtc acctaaatag cttggcgtaa tcatggtcat
     5401 agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa
     5461 gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc
     5521 gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc
     5581 aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact
     5641 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
     5701 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
     5761 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
     5821 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     5881 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     5941 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
     6001 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     6061 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     6121 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     6181 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa
     6241 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
     6301 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
     6361 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
     6421 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
     6481 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
     6541 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
     6601 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
     6661 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
     6721 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
     6781 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
     6841 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt
     6901 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
     6961 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
     7021 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
     7081 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
     7141 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca
     7201 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
     7261 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
     7321 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
     7381 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
     7441 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
     7501 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac gtctaagaaa
     7561 ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc tttcgtctcg
     7621 cgcgtttcgg tgatgacggt gaaaacctct gacacatgca gctcccggag acggtcacag
     7681 cttgtctgta agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg
     7741 gcgggtgtcg gggctggctt aactatgcgg catcagagca gattgtactg agagtgcacc
     7801 atatgcggtg tgaaataccg cacagatgcg taaggagaaa ataccgcatc aggaaattgt
     7861 aagcgttaat attttgttaa aattcgcgtt aaatttttgt taaatcagct cattttttaa
     7921 ccaataggcc gaaatcggca aaatccctta taaatcaaaa gaatagaccg agatagggtt
     7981 gagtgttgtt ccagtttgga acaagagtcc actattaaag aacgtggact ccaacgtcaa
     8041 agggcgaaaa accgtctatc agggcgatgg cccactacgt gaaccatcac cctaatcaag
     8101 ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga gcccccgatt
     8161 tagagcttga cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg
     8221 agcgggcgct agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc
     8281 cgcgcttaat gcgccgctac agggcgcgtc cattcgccat tcaggctgcg caactgttgg
     8341 gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct
     8401 gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg taaaacgacg
     8461 gccagtgaat tgtaatacga ctcactata
//
