LOCUS       Exported                4416 bp DNA     circular SYN 22-JUL-2021
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4416)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 22, 2021 from SnapGene 5.3.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4416
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    4..69
                     /label=X. laevis beta globin 5'UTR
                     /note="X. laevis beta globin 5'UTR"
     CDS             70..786
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             787..810
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             811..837
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     misc_feature    841..970
                     /label=X. laevis beta globin 3'-UTR
                     /note="X. laevis beta globin 3'-UTR"
     misc_feature    990..1089
                     /label=polyA
                     /note="polyA"
     misc_feature    1090..1192
                     /label=mouse MALAT1 6588-6690
                     /note="mouse MALAT1 6588-6690"
     misc_feature    1193..1260
                     /label=HDV ribozyme short from p2RZ
                     /note="HDV ribozyme short from p2RZ"
     misc_feature    complement(1286..1305)
                     /label=SP6 promoter
                     /note="SP6 promoter"
     misc_feature    join(4400..4416,1..3)
                     /label=T7 promoter
                     /note="T7 promoter"
ORIGIN
        1 ggggaataca agcttcttgt tctttttgca gaagctcaga ataaacgctc aactttggca
       61 gatctgaaca tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc
      121 gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat
      181 gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc
      241 tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt gcttcagccg ctaccccgac
      301 cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc
      361 accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc
      421 gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc
      481 ctggggcaca agctggagta caactacaac agccacaacg tctatatcat ggccgacaag
      541 cagaagaacg gcatcaaggt gaacttcaag atccgccaca acatcgagga cggcagcgtg
      601 cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc
      661 gacaaccact acctgagcac ccagtccgcc ctgagcaaag accccaacga gaagcgcgat
      721 cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg
      781 tacaaggact acaaggacga tgacgataag tatccatatg atgttccaga ttatgcttaa
      841 accagcctca agaacacccg aatggagtct ctaagctaca taataccaac ttacacttta
      901 caaaatgttg tcccccaaaa tgtagccatt cgtatctgct cctaataaaa agaaagtttc
      961 ttcacattct gggcgtagat ccagacatga aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
     1021 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
     1081 aaaaaaaaac agtagggttg taaaggtttt tcttttcctg agaaaacaac cttttgtttt
     1141 ctcaggtttt gctttttggc ctttccctag ctttaaaaaa aaaaaagcaa aagctagcca
     1201 tggtcccagc ctcctcgctg gcggctagtg ggcaacatgc ttcggcatgg cgaatgggac
     1261 gcggccgcat gcataagctt gagtattcta tagtgtcacc taaatagctt ggcgtaatca
     1321 tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga
     1381 gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt
     1441 gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga
     1501 atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc
     1561 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
     1621 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
     1681 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
     1741 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
     1801 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
     1861 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
     1921 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
     1981 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     2041 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
     2101 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
     2161 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
     2221 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
     2281 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
     2341 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
     2401 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
     2461 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
     2521 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
     2581 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
     2641 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
     2701 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
     2761 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
     2821 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
     2881 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
     2941 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
     3001 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
     3061 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
     3121 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
     3181 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
     3241 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
     3301 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
     3361 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
     3421 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc
     3481 taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt
     3541 cgtctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg
     3601 gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg
     3661 ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat cagagcagat tgtactgaga
     3721 gtgcaccata tgcggtgtga aataccgcac agatgcgtaa ggagaaaata ccgcatcagg
     3781 aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat
     3841 tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa tagaccgaga
     3901 tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac gtggactcca
     3961 acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa ccatcaccct
     4021 aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct aaagggagcc
     4081 cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag
     4141 cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca
     4201 cacccgccgc gcttaatgcg ccgctacagg gcgcgtccat tcgccattca ggctgcgcaa
     4261 ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg
     4321 atgtgctgca aggcgattaa gttgggtaac gccagggttt tcccagtcac gacgttgtaa
     4381 aacgacggcc agtgaattgt aatacgactc actata
//
