LOCUS       Exported                6127 bp ds-DNA     circular SYN 09-FEB-2021
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 6127)
  AUTHORS   MIT
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Jul 22, 2021 from SnapGene 4.3.10
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6127
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     misc_feature    1..130
                     /locus_tag="L-ITR"
                     /label=L-ITR
                     /note="/vntifkey=21"
                     /note="color: #c0c0c0"
     misc_feature    14..29
                     /locus_tag="AAV REP78 binding element"
                     /label=AAV REP78 binding element
                     /note="color: #ff8000"
     misc_feature    38..46
                     /locus_tag="AAV Hairpin element 2(1)"
                     /label=AAV Hairpin element 2
                     /label=AAV Hairpin element 2(1)
                     /note="color: #ff8000"
     misc_feature    complement(47)
                     /locus_tag="AAV Hairpin element 2(3)"
                     /label=AAV Hairpin element 2
                     /label=AAV Hairpin element 2(3)
                     /note="color: #ff8000; direction: LEFT"
     misc_feature    49..57
                     /locus_tag="AAV Hairpin element 1"
                     /label=AAV Hairpin element 1
                     /note="color: #ff8000"
     misc_feature    complement(61..69)
                     /locus_tag="AAV Hairpin element 1(1)"
                     /label=AAV Hairpin element 1
                     /label=AAV Hairpin element 1(1)
                     /note="color: #ff8000; direction: LEFT"
     misc_feature    complement(78..93)
                     /locus_tag="AAV REP78 binding element(1)"
                     /label=AAV REP78 binding element
                     /label=AAV REP78 binding element(1)
                     /note="color: #ff8000; direction: LEFT"
     misc_feature    110..130
                     /locus_tag="AAV D' element"
                     /label=AAV D' element
                     /note="color: #ff8000"
     misc_feature    138..455
                     /locus_tag="TRE-tight"
                     /label=TRE-tight
                     /note="color: #ccff66"
     CDS             206..455
                     /codon_start=1
                     /locus_tag="ORF frame 3"
                     /label=ORF frame 3
                     /note="color: #ffc0cb"
                     /translation="MSSLLPISDRERMSSLLPISDRERMSSLLPISDRERMSSLSLSVI
                     ENVCRVYSLSVIENVCRGRRVRWEAYISRARLVNRQIA"
     primer_bind     377..396
                     /label=TRE-seq1-fp_53
                     /note="color: black; sequence: GAGAACGTATGTCGAGGTAG; added:
                     2021-02-07"
     misc_feature    425..444
                     /locus_tag="pCEP_fwd_primer"
                     /label=pCEP_fwd_primer
                     /note="/translation=MSSLLPISDRERMSSLLPISDRERMSSLLPISDRERMSS
                     LSLSV 
                     IENVCRVYSLSVIENVCRGRRVRWEAYISRARLVNRQIAWRILAATMPEPAKSAPAPK 
                     KGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKVLKQVHPDTGISSKAMGIMNSFVNDI 
                     FERIAGEASRLAHYNKRSTITSREIQTAVRLLLPGELAKHAVSEGTKAITKYTSAKDP 
                     PVATMVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKL 
                     PVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAE 
                     VKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRH 
                     NIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAA 
                     GITLGMDELYKGSGVKQTLNFDLLKLAGDVESNPGPMARLLPALLLLLLPGNVTGNGS 
                     GNGSLSRCPPGQFRCSEPPGAHGECYPQDWLCDGHPDCDDGRDEWGCGTSAIPAVPTD 
                     NGTEAPTAPAPGRALPARNHGRMWMLITAVLLCCLVAVGGIAAWGKSKAKSRSDIFSL 
                     ESASKELLVPDKSQADLFSGSGEGRGSLLTCGDVEENPGPMVPQALLFVPLLVFPLCF 
                     GKFPIYTIPDKLGPWSPIDIHHLSCPNNLVVEDEGCTNLSGFSYMELKVGYILAIKVN 
                     GFTCTGVVTEAETYTNFVGYVTTTFKRKHFRPTPDACRAAYNWKMAGDPRYEESLHNP 
                     YPDYRWLRTVKTTKESLVIISPSVADLDPYDRSLHSRVFPSGKCSGVAVSSTYCSTNH 
                     DYTIWMPENPRLGMSCDIFTNSRGKRASKGSETCGFVDERGLYKSLKGACKLKLCGVL 
                     GLRLMDGTWVSMQTSNETKWCPPDKLVNLHDFRSDEIEHLVVEELVRKREECLDALES 
                     IMTTKSVSFRRLSHLRKLVPGFGKAYTIFNKTLMEADAHYKSVRTWNEILPSKGCLRV 
                     GGRCHPHVNGVFFNGIILGPDGNVLIPEMQSSLLQQHMELLESSVIPLVHPLADPSTV 
                     FKDGDEAEDFVEVHLPDVHNQVSGVDLGLPNWGKYVLLSAGALTALMLIIFLMTCCRR 
                     VNRSEPTQHNLRGTGREVSVTPQSGKIISSWESHKSGGETRL*"
                     /note="color: #ffc0cb"
     misc_feature    427..451
                     /locus_tag="LNCX_primer"
                     /label=LNCX_primer
                     /note="/translation=MSSLLPISDRERMSSLLPISDRERMSSLLPISDRERMSS
                     LSLSV 
                     IENVCRVYSLSVIENVCRGRRVRWEAYISRARLVNRQIAWRILAATMPEPAKSAPAPK 
                     KGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKVLKQVHPDTGISSKAMGIMNSFVNDI 
                     FERIAGEASRLAHYNKRSTITSREIQTAVRLLLPGELAKHAVSEGTKAITKYTSAKDP 
                     PVATMVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKL 
                     PVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAE 
                     VKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRH 
                     NIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAA 
                     GITLGMDELYKGSGVKQTLNFDLLKLAGDVESNPGPMARLLPALLLLLLPGNVTGNGS 
                     GNGSLSRCPPGQFRCSEPPGAHGECYPQDWLCDGHPDCDDGRDEWGCGTSAIPAVPTD 
                     NGTEAPTAPAPGRALPARNHGRMWMLITAVLLCCLVAVGGIAAWGKSKAKSRSDIFSL 
                     ESASKELLVPDKSQADLFSGSGEGRGSLLTCGDVEENPGPMVPQALLFVPLLVFPLCF 
                     GKFPIYTIPDKLGPWSPIDIHHLSCPNNLVVEDEGCTNLSGFSYMELKVGYILAIKVN 
                     GFTCTGVVTEAETYTNFVGYVTTTFKRKHFRPTPDACRAAYNWKMAGDPRYEESLHNP 
                     YPDYRWLRTVKTTKESLVIISPSVADLDPYDRSLHSRVFPSGKCSGVAVSSTYCSTNH 
                     DYTIWMPENPRLGMSCDIFTNSRGKRASKGSETCGFVDERGLYKSLKGACKLKLCGVL 
                     GLRLMDGTWVSMQTSNETKWCPPDKLVNLHDFRSDEIEHLVVEELVRKREECLDALES 
                     IMTTKSVSFRRLSHLRKLVPGFGKAYTIFNKTLMEADAHYKSVRTWNEILPSKGCLRV 
                     GGRCHPHVNGVFFNGIILGPDGNVLIPEMQSSLLQQHMELLESSVIPLVHPLADPSTV 
                     FKDGDEAEDFVEVHLPDVHNQVSGVDLGLPNWGKYVLLSAGALTALMLIIFLMTCCRR 
                     VNRSEPTQHNLRGTGREVSVTPQSGKIISSWESHKSGGETRL*"
                     /note="color: #ffc0cb"
     CDS             456..458
                     /codon_start=1
                     /locus_tag="ORF frame 3(1)"
                     /label=ORF frame 3
                     /label=ORF frame 3(1)
                     /note="color: #ffc0cb"
                     /translation="M"
     misc_feature    465..467
                     /locus_tag="AscI site"
                     /label=AscI site
                     /note="color: #0000ff"
     misc_feature    474..1787
                     /locus_tag="H2b-emiRFP670 (Matlashov...& Verkhusha '20)"
                     /label=H2b-emiRFP670 (Matlashov...& Verkhusha '20)
                     /label=H2b-emiRFP670 (Matlashov...& Verkhusha '20)
                     /note="color: #00ffff"
     CDS             474..851
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /locus_tag="H2B"
                     /product="human histone H2B"
                     /label=H2B
                     /note="color: #e9d024"
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAITKYTSAK"
     misc_feature    852..860
                     /label=Linker
                     /note="color: #a6acb3"
     CDS             861..1787
                     /codon_start=1
                     /label=emiRFP670
                     /note="color: #993366"
                     /translation="AEGSVARQPDLLTCEHEEIHLAGSIQPHGALLVVSEHDHRVIQAS
                     ANAAEFLNLGSVLGVPLAEIDGDLLIKILPHLDPTAEGMPVAVRCRIGNPSTEYCGLMH
                     RPPEGGLIIELERAGPSIDLSGTLAPALERIRTAGSLRALCDDTVLLFQQCTGYDRVMV
                     YRFDEQGHGLVFSECHVPGLESYFGNRYPSSTVPQMARQLYVRQRVRVLVDVTYQPVPL
                     EPRLSPLTGRDLDMSGCFLRSMSPCHLQFLKDMGVRATLAVSLVVGGKLWGLVVCHHYL
                     PRFIRFELRAICKRLAERIATRITALES"
     regulatory      861
                     /regulatory_class="other"
                     /locus_tag="vertebrate consensus sequence for strong 
                     initiation of translation (Kozak, 1987)"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /note="/label=vertebrate consensus sequence for strong 
                     initiation of translation (Kozak, 1987)"
                     /note="color: #ffc0cb"
     primer_bind     1080..1095
                     /label=emiRFP670-seq2-fp_55
                     /note="color: black; sequence: ctgccgcatctcgatc; added: 
                     2021-02-07"
     misc_feature    1788..1853
                     /locus_tag="P2A"
                     /label=P2A
                     /note="color: #ffff00"
     CDS             1854..2561
                     /codon_start=1
                     /locus_tag="TEVP S129V from pAAV-flex-taCasp3-TEVp 
                     (Yang...& Shah '13)"
                     /label=TEVP S129V from pAAV-flex-taCasp3-TEVp (Yang......
                     /label=TEVP S129V from pAAV-flex-taCasp3-TEVp (Yang...& 
                     Shah '13)
                     /note="color: #0dff00"
                     /translation="MGESLFKGPRDYNPISSTICHLTNESDGHTTSLYGIGFGPFIITN
                     KHLFRRNNGTLLVQSLHGVFKVKNTTTLQQHLIDGRDMIIIRMPKDFPPFPQKLKFREP
                     QREERICLVTTNFQTKSMSSMVSDTSCTFPSSDGIFWKHWIQTKDGQCGSPLVSTRDGF
                     IVGIHSASNFTNTNNYFTSVPKNFMELLTNQEAQQWVSGWRLNADSVLWGGHKVFMVKP
                     EEPFQPVKEATQL"
     misc_feature    2562..2567
                     /locus_tag="NheI site"
                     /label=NheI site
                     /note="color: #0028f9"
     misc_feature    2568..3159
                     /locus_tag="WPRE (match to WHV8 genome, J04514)"
                     /label=WPRE (match to WHV8 genome, J04514)
                     /note="color: #800000"
     primer_bind     complement(2618..2637)
                     /label=WPRE-seq3-rp_55
                     /note="color: black; sequence: gcgtaaaaggagcaacatag; added:
                     2021-02-07"
     misc_feature    3160..3164
                     /locus_tag="BamHI site"
                     /label=BamHI site
                     /note="color: #0000ff"
     misc_feature    3164..3388
                     /locus_tag="BGH pA"
                     /label=BGH pA
                     /note="color: #ffc000"
     misc_feature    3390..3530
                     /locus_tag="R-ITR"
                     /label=R-ITR
                     /note="/vntifkey=21"
                     /note="color: #c0c0c0"
     misc_feature    complement(3390..3410)
                     /locus_tag="AAV D' element(1)"
                     /label=AAV D' element
                     /label=AAV D' element(1)
                     /note="color: #ff8000; direction: LEFT"
     misc_feature    3427..3442
                     /locus_tag="AAV REP78 binding element(2)"
                     /label=AAV REP78 binding element
                     /label=AAV REP78 binding element(2)
                     /note="color: #ff8000"
     misc_feature    3451..3459
                     /locus_tag="AAV Hairpin element 1(2)"
                     /label=AAV Hairpin element 1
                     /label=AAV Hairpin element 1(2)
                     /note="color: #ff8000"
     misc_feature    complement(3463..3471)
                     /locus_tag="AAV Hairpin element 1(3)"
                     /label=AAV Hairpin element 1
                     /label=AAV Hairpin element 1(3)
                     /note="color: #ff8000; direction: LEFT"
     misc_feature    3473..3481
                     /locus_tag="AAV Hairpin element 2"
                     /label=AAV Hairpin element 2
                     /note="color: #ff8000"
     misc_feature    complement(3485..3493)
                     /locus_tag="AAV Hairpin element 2(2)"
                     /label=AAV Hairpin element 2
                     /label=AAV Hairpin element 2(2)
                     /note="color: #ff8000; direction: LEFT"
     misc_feature    complement(3502..3517)
                     /locus_tag="AAV REP78 binding element(3)"
                     /label=AAV REP78 binding element
                     /label=AAV REP78 binding element(3)
                     /note="color: #ff8000; direction: LEFT"
     rep_origin      3605..4060
                     /locus_tag="M13 origin"
                     /label=M13 origin
                     /note="color: #808080"
     rep_origin      3622..3928
                     /locus_tag="f1\Origin"
                     /label=f1\Origin
                     /note="/vntifkey=33"
                     /note="color: #ffc0cb"
     rep_origin      3622..3928
                     /locus_tag="F1 ori"
                     /label=F1 ori
                     /note="color: #808080"
     misc_feature    4447..5304
                     /locus_tag="AmpR"
                     /label=AmpR
                     /note="/vntifkey=21"
                     /note="color: #c0c0c0"
     CDS             4645..5304
                     /locus_tag="AmpR(1)"
                     /label=AmpR
                     /label=AmpR(1)
                     /note="color: #ffff00; direction: RIGHT"
     rep_origin      5402..6084
                     /locus_tag="ColE1 origin"
                     /label=ColE1 origin
                     /note="color: #808080"
     rep_origin      5455..6122
                     /locus_tag="pUC\ori"
                     /label=pUC\ori
                     /note="/vntifkey=33"
                     /note="color: #ffc0cb"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct acgcgtctcg agtttactcc ctatcagtga tagagaacgt atgtcgagtt
      181 tactccctat cagtgataga gaacgatgtc gagtttactc cctatcagtg atagagaacg
      241 tatgtcgagt ttactcccta tcagtgatag agaacgtatg tcgagtttac tccctatcag
      301 tgatagagaa cgtatgtcga gtttatccct atcagtgata gagaacgtat gtcgagttta
      361 ctccctatca gtgatagaga acgtatgtcg aggtaggcgt gtacggtggg aggcctatat
      421 aagcagagct cgtttagtga accgtcagat cgcctggaga attcgccgcc accatgccag
      481 agccagcgaa gtctgctccc gccccgaaaa agggctccaa gaaggcggtg actaaggcgc
      541 agaagaaagg cggcaagaag cgcaagcgca gccgcaagga gagctattcc atctatgtgt
      601 acaaggttct gaagcaggtc caccctgaca ccggcatttc gtccaaggcc atgggcatca
      661 tgaattcgtt tgtgaacgac attttcgagc gcatcgcagg tgaggcttcc cgcctggcgc
      721 attacaacaa gcgctcgacc atcacctcca gggagatcca gacggccgtg cgcctgctgc
      781 tgcctgggga gttggccaag cacgccgtgt ccgagggtac taaggccatc accaagtaca
      841 ccagcgctaa gggcagcggc gcggaaggct ccgtcgccag gcagcctgac ctcttgacct
      901 gcgaacatga agagatccac ctcgccggct cgatccagcc gcatggcgcg cttctggtcg
      961 tcagcgaaca tgatcatcgc gtcatccagg ccagcgccaa cgccgcggaa tttctgaatc
     1021 tcggaagcgt actcggcgtt ccgctcgccg agatcgacgg cgatctgttg atcaagatcc
     1081 tgccgcatct cgatcccacc gccgaaggca tgccggtcgc ggtgcgctgc cggatcggca
     1141 atccctctac ggagtactgc ggtctgatgc atcggcctcc ggaaggcggg ctgatcatcg
     1201 aactcgaacg tgccggcccg tcgatcgatc tgtcaggcac gctggcgccg gcgctggagc
     1261 ggatccgcac ggcgggttca ctgcgcgcgc tgtgcgatga caccgtgctg ctgtttcagc
     1321 agtgcaccgg ctacgaccgg gtgatggtgt atcgtttcga tgagcaaggc cacggcctgg
     1381 tattctccga gtgccatgtg cctgggctcg aatcctattt cggcaaccgc tatccgtcgt
     1441 cgactgtccc gcagatggcg cggcagctgt acgtgcggca gcgcgtccgc gtgctggtcg
     1501 acgtcaccta tcagccggtg ccgctggagc cgcggctgtc gccgctgacc gggcgcgatc
     1561 tcgacatgtc gggctgcttc ctgcgctcga tgtcgccgtg ccatctgcag ttcctgaagg
     1621 acatgggcgt gcgcgccacc ctggcggtgt cgctggtggt cggcggcaag ctgtggggcc
     1681 tggttgtctg tcaccattat ctgccgcgct tcatccgttt cgagctgcgg gcgatctgca
     1741 aacggctcgc cgaaaggatc gcgacgcgga tcaccgcgct tgagagcgga agcggagcta
     1801 ctaacttcag cctgctgaag caggctggag acgtggagga gaaccctgga cctatgggcg
     1861 agagcctttt caagggcccg agggactaca acccgatctc cagcaccatc tgtcacctga
     1921 ccaacgagag cgacggtcac accactagtc tgtacggcat cggcttcggc cccttcatca
     1981 tcaccaacaa gcatctgttc aggaggaata acggcacact gctggtgcaa agcctgcacg
     2041 gcgtgttcaa agtgaagaac acaaccaccc tgcaacagca cctgatcgac ggcagggaca
     2101 tgattatcat caggatgccc aaggacttcc ccccctttcc ccagaaactg aagttcaggg
     2161 agccacaaag ggaggagcga atctgcctgg tgaccaccaa cttccagacc aagtccatga
     2221 gcagcatggt ctctgatacc agctgcacct tccccagcag cgacggcatc ttctggaagc
     2281 actggattca gacgaaggat ggccaatgcg gcagcccatt ggtgagcact agggacggct
     2341 tcatcgtggg catccacagc gccagcaatt ttaccaatac caacaactac ttcacgagcg
     2401 tgccgaaaaa cttcatggag ctgttgacca atcaagaggc gcagcagtgg gtgagcggct
     2461 ggaggctgaa cgccgacagc gttctttggg gcggacataa ggtgttcatg gtcaagcccg
     2521 aggaaccctt ccagcccgtt aaggaagcca ctcagctttg agctagcaat caacctctgg
     2581 attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat
     2641 gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt
     2701 tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca
     2761 ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg
     2821 ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg
     2881 aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca
     2941 attccgtggt gttgtcgggg aagctgacgt cctttccatg gctgctcgcc tgtgttgcca
     3001 cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc
     3061 ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc
     3121 agacgagtcg gatctccctt tgggccgcct ccccgcctgg atcctgtgcc ttctagttgc
     3181 cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc
     3241 actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct
     3301 attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga caatagcagg
     3361 catgctgggg atgcggtggg ctctatggca ggaaccccta gtgatggagt tggccactcc
     3421 ctctctgcgc gctcgctcgc tcactgaggc cgggcgacca aaggtcgccc gacgcccggg
     3481 ctttgcccgg gcggcctcag tgagcgagcg agcgcgcagc tgcctgcagg ggcgcctgat
     3541 gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatacgtc aaagcaacca
     3601 tagtacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg
     3661 accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc
     3721 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga
     3781 tttagtgctt tacggcacct cgaccccaaa aaacttgatt tgggtgatgg ttcacgtagt
     3841 gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat
     3901 agtggactct tgttccaaac tggaacaaca ctcaacccta tctcgggcta ttcttttgat
     3961 ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa
     4021 tttaacgcga attttaacaa aatattaacg tttacaattt tatggtgcac tctcagtaca
     4081 atctgctctg atgccgcata gttaagccag ccccgacacc cgccaacacc cgctgacgcg
     4141 ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg
     4201 agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc
     4261 gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt
     4321 ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca
     4381 aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg
     4441 aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc
     4501 cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg
     4561 ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt
     4621 cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta
     4681 ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat
     4741 gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga
     4801 gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca
     4861 acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact
     4921 cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc
     4981 acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact
     5041 ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt
     5101 ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt
     5161 gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt
     5221 atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata
     5281 ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag
     5341 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat
     5401 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
     5461 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     5521 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     5581 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg
     5641 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     5701 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     5761 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     5821 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
     5881 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     5941 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     6001 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     6061 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
     6121 cacatgt
//
