LOCUS       Exported               15000 bp ds-DNA     circular SYN 07-MAR-2021
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 15000)
  AUTHORS   MIT
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Jul 22, 2021 from SnapGene 4.3.10
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..15000
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     primer_bind     213..232
                     /label=pRV_213_fsp
                     /note="8.7.5"
                     /note="color: black; sequence: TTGATTAGGGTGATGGTTCA; added:
                     2021-02-07"
     misc_feature    652..1242
                     /label=CMV promoter
                     /note="CMV promotor"
                     /note="color: #ff8000"
     misc_feature    1285..1305
                     /label=T7 promoter
                     /note="T7 promotor"
                     /note="color: #ff8000"
     misc_feature    1324..1370
                     /label=HH ribozyme
                     /note="HH ribozyme"
                     /note="color: #800080"
     misc_feature    1371..1428
                     /label=SAD B19 leader
                     /note="RV leader"
                     /note="color: #ff80ff"
     primer_bind     complement(1419..1437)
                     /label=N2cN_Tran_start_rsp
                     /note="9.3.3"
                     /note="color: black; sequence: AGGGGTGTTacatttttgc; added: 
                     2021-02-07"
     misc_feature    1429..1437
                     /label=Transcriptional start (N)
                     /note="color: #ffff00"
     CDS             1441..2361
                     /codon_start=1
                     /label=N
                     /note="N"
                     /note="color: #00ff00"
                     /translation="MDADKIVFKVNNQVVSLKPEIIVDQYEYKYPAIKDLKKPCITLGK
                     APDLNKAYKSVLSGMSAAKLNPDDVCSYLAAAMQFFEGTCPEDWTSYGIVIARKGDKIT
                     PGSLVEIKRTDVEGNWALTGGMELTRDPTVPEHASLVGLLLSLYRLSKISGQNTGNYKT
                     NIADRIEQIFETAPFVKIVEHHTLMTTHKMCANWSTIPNFRFLAGTYDMFFSRIEHLYS
                     AIRVGTVVTAYEDCSGLVSFTGFIKQINLTAREAILYFFHKNFEEEIRRMFEPGQETAV
                     PHSYFIHFRSLGLSGKSPYSSNAVGH"
     primer_bind     1472..1491
                     /label=N_31_fsp
                     /note="2.1.1"
                     /note="color: black; sequence: ATAATCAGGTGGTCTCTTTG; added:
                     2021-02-07"
     primer_bind     complement(1648..1667)
                     /label=N_207_rsp
                     /note="1.3.8"
                     /note="color: black; sequence: GCTGCCAAATAGGAACATAC; added:
                     2021-02-07"
     primer_bind     2235..2256
                     /label=N_794_fsp
                     /note="2.1.2"
                     /note="color: black; sequence: TGAGGAAGAGATAAGAAGaatg; 
                     added: 2021-02-07"
     CDS             2337..2790
                     /codon_start=2
                     /locus_tag="N"
                     /label=N
                     /note="N"
                     /note="color: #e9d024"
                     /translation="YSSNAVGHVFNLIHFVGCYMGQVRSLNATVIAACAPHEMSVLGGY
                     LGEEFFGKGTFERRFFRDEKELQEYEAAELTKTDVALADDGTVNSDDEDYFSGETRSPE
                     AVYTRIMMNGGRLKRSHIRRYVSVSSNHQARPNSFAEFLNKTYSSDS"
     misc_feature    2337..2787
                     /locus_tag="SAD B19 N apart from stop codon"
                     /label=SAD B19 N apart from stop codon
                     /note="color: #0dff00"
     primer_bind     2342..2359
                     /label=B19N_902_fsp
                     /note="8.7.3"
                     /note="color: black; sequence: CATCAAATGCTGTTGGTC; added: 
                     2021-02-07"
     primer_bind     2347..2364
                     /label=PmlI_fp_56
                     /note="color: black; sequence: AATGCTGTTGGTCACGTG; added: 
                     2021-02-07"
     primer_bind     2605..2658
                     /label=Adaptor-UMI-N_fp_57
                     /note="color: black; sequence: 
                     ACACTCTTTCCCTACACGACGCTCTTCCGATCTNNNNNNNNNNNNNNNNNNNNAGAAGT
                     CCGGAGGCTGTTTAT; added: 2021-03-08"
     primer_bind     2681..2706
                     /label=BglII_fp_58
                     /note="color: black; sequence: GTCGACTAAAGAGATCTCACATACGG; 
                     added: 2021-02-07"
     misc_feature    2778
                     /locus_tag="point mutation could cause stop codon(7)"
                     /label=point mutation could cause stop codon
                     /label=point mutation could cause stop codon(7)
                     /note="color: #ff0000"
     misc_feature    2779..2781
                     /label=Silent mutation
                     /note="color: #ff00ff"
     misc_feature    2788..2790
                     /label=Silent mutation
                     /note="color: #ff00ff"
     misc_feature    2791..2799
                     /locus_tag="linker"
                     /label=linker
                     /note="color: #6b60ff"
     misc_feature    2797..2799
                     /label=Silent mutation
                     /note="color: #ff00ff"
     CDS             2800..2820
                     /codon_start=1
                     /locus_tag="TEV site"
                     /label=TEV site
                     /note="TEV site"
                     /note="color: #00ffff"
                     /translation="ENLYFQS"
     misc_feature    2800
                     /locus_tag="point mutation could cause stop codon"
                     /label=point mutation could cause stop codon
                     /note="color: #ff0000"
     misc_feature    2811
                     /locus_tag="point mutation could cause stop codon(2)"
                     /label=point mutation could cause stop codon
                     /label=point mutation could cause stop codon(2)
                     /note="color: #ff0000"
     misc_feature    2815
                     /locus_tag="point mutation could cause stop codon(3)"
                     /label=point mutation could cause stop codon
                     /label=point mutation could cause stop codon(3)
                     /note="color: #ff0000"
     misc_feature    2818..2820
                     /label=Silent mutation
                     /note="color: #ff00ff"
     misc_feature    2821..2829
                     /locus_tag="linker(1)"
                     /label=linker
                     /label=linker(1)
                     /note="color: #6b60ff"
     misc_feature    2821..2823
                     /label=Silent mutation
                     /note="color: #ff00ff"
     CDS             2830..2949
                     /codon_start=1
                     /locus_tag="ECFP destabilized by fusion to residues 422-461
                     of mouse ornithine decarboxylase, giving an in vivo(1)"
                     /product="ECFP destabilized by fusion to residues 422-461 
                     of mouse ornithine decarboxylase, giving an in vivo 
                     half-life of ~2 hours"
                     /label=ECFP destabilized by fusion to residues 422-461...
                     /label=ECFP destabilized by fusion to residues 422-461 of 
                     mouse ornithine decarboxylase, giving an in vivo(1)
                     /note="destabilization domain"
                     /note="mammalian codon-optimized"
                     /note="color: #ffff00"
                     /translation="SHGFPPEVEEQDDGTLPMSCAQESGMDRHPAACASARINV"
     misc_feature    2848
                     /locus_tag="point mutation could cause stop codon(9)"
                     /label=point mutation could cause stop codon
                     /label=point mutation could cause stop codon(9)
                     /note="color: #ff0000"
     misc_feature    2854
                     /locus_tag="point mutation could cause stop codon(10)"
                     /label=point mutation could cause stop codon
                     /label=point mutation could cause stop codon(10)
                     /note="color: #ff0000"
     misc_feature    2857
                     /locus_tag="point mutation could cause stop codon(11)"
                     /label=point mutation could cause stop codon
                     /label=point mutation could cause stop codon(11)
                     /note="color: #ff0000"
     misc_feature    2860
                     /locus_tag="point mutation could cause stop codon(12)"
                     /label=point mutation could cause stop codon
                     /label=point mutation could cause stop codon(12)
                     /note="color: #ff0000"
     misc_feature    2889
                     /locus_tag="point mutation could cause stop codon(13)"
                     /label=point mutation could cause stop codon
                     /label=point mutation could cause stop codon(13)
                     /note="color: #ff0000"
     misc_feature    2893
                     /locus_tag="point mutation could cause stop codon(14)"
                     /label=point mutation could cause stop codon
                     /label=point mutation could cause stop codon(14)
                     /note="color: #ff0000"
     misc_feature    2896
                     /locus_tag="point mutation could cause stop codon(15)"
                     /label=point mutation could cause stop codon
                     /label=point mutation could cause stop codon(15)
                     /note="color: #ff0000"
     misc_feature    2928
                     /locus_tag="point mutation could cause stop codon(16)"
                     /label=point mutation could cause stop codon
                     /label=point mutation could cause stop codon(16)
                     /note="color: #ff0000"
     misc_feature    2950..2952
                     /locus_tag="stop codon"
                     /label=stop codon
                     /note="color: #0dff00"
     misc_feature    2953..3042
                     /locus_tag="region between N and P genes in SAD B19 genome"
                     /label=region between N and P genes in SAD B19 genome
                     /label=region between N and P genes in SAD B19 genome
                     /note="color: #fe5aff"
     primer_bind     complement(2953..2976)
                     /label=Barcode-N_rp_56
                     /note="color: black; sequence: TCCGGCATTTTGTTATTCAACTTC; 
                     added: 2021-02-07"
     primer_bind     complement(2953..2976)
                     /label=NheI_rp_56
                     /note="color: black; sequence: 
                     AGATTGCTAGCTCCGGCATTTTGTTATTCAACTTC; added: 2021-02-07"
     primer_bind     2953..2973
                     /label=pRV_after_B19N_fsp
                     /note="8.7.6"
                     /note="color: black; sequence: GAAGTTGAATAACAAAATGCC; 
                     added: 2021-02-07"
     primer_bind     complement(2956..2976)
                     /label=N_Barcode_NheI_rp_59
                     /note="color: black; sequence: AGCTCCGGCATTTTGTTATTCAAC; 
                     added: 2021-02-07"
     primer_bind     2977..3001
                     /label=N_Barcode_NheI_fp_58
                     /note="color: black; sequence: 
                     AGCAATCTACGGATTGTGTATATCCATC; added: 2021-02-07"
     primer_bind     complement(3001..3021)
                     /label=B19N_Trans_end_rsp
                     /note="8.7.4"
                     /note="color: black; sequence: GGGGTGTTAGTTTTTTTCATG; 
                     added: 2021-02-07"
     primer_bind     complement(3013..3032)
                     /label=i7-anchor_GGTTATAA_rp_54
                     /note="color: black; sequence: 
                     CAAGCAGAAGACGGCATACGAGATTTATAACCGTGACTGGAGTTCAGACGTGTGCTCTT
                     CCGATCTGTTCGAAAGGAGGGGTGTTA; added: 2021-03-08"
     primer_bind     complement(3013..3032)
                     /label=i7-anchor_AACCGCGG_rp_54
                     /note="color: black; sequence: 
                     CAAGCAGAAGACGGCATACGAGATCCGCGGTTGTGACTGGAGTTCAGACGTGTGCTCTT
                     CCGATCTGTTCGAAAGGAGGGGTGTTA; added: 2021-03-08"
     primer_bind     complement(3021..3041)
                     /label=i7-anchor_GGTTATAA_rp_56
                     /note="color: black; sequence: 
                     CAAGCAGAAGACGGCATACGAGATTTATAACCGTGACTGGAGTTCAGACGTGTGCTCTT
                     CCGATCTTTGGGATGGTTCGAAAGGAG; added: 2021-03-08"
     primer_bind     complement(3021..3040)
                     /label=i7-anchor_AACCGCGG_rp_56
                     /note="color: black; sequence: 
                     CAAGCAGAAGACGGCATACGAGATCCGCGGTTGTGACTGGAGTTCAGACGTGTGCTCTT
                     CCGATCTTGGGATGGTTCGAAAGGAG; added: 2021-03-08"
     primer_bind     complement(3022..3042)
                     /label=BstBI_rp_57
                     /note="color: black; sequence: GTTTGGGATGGTTCGAAAGGA; 
                     added: 2021-02-07"
     primer_bind     complement(3042..3066)
                     /label=BglII_rp_58
                     /note="color: black; sequence: AGGATTGACAAAGATCTTGCTCATG; 
                     added: 2021-02-07"
     misc_feature    3043..3936
                     /label=P
                     /note="P"
                     /note="color: #00ff00"
     CDS             3043..3049
                     /codon_start=1
                     /locus_tag="P"
                     /label=P
                     /note="P"
                     /note="color: #e9d024"
                     /translation="MS"
     primer_bind     complement(3137..3154)
                     /label=B19P_95_rsp
                     /note="8.7.8"
                     /note="color: black; sequence: GAGCCTGATTGTCTTCGA; added: 
                     2021-02-07"
     primer_bind     3143..3160
                     /label=P_100_fsp
                     /note="2.1.3"
                     /note="color: black; sequence: ACAATCAGGCTCATCTCC; added: 
                     2021-02-07"
     primer_bind     complement(3214..3231)
                     /label=D_Sanger_rp_Lei
                     /note="color: green; sequence: 
                     CAAGCAGAAGACGGCACGATTTTCCATCATCCAG; added: 2021-03-08"
     misc_feature    3994..4004
                     /label=Transcription stop/pA signal (N)
                     /note="color: #ffff00"
     misc_feature    4010..4018
                     /label=Transcriptional start (M)
                     /note="color: #ffff00"
     misc_feature    4025..4633
                     /label=M
                     /note="M"
                     /note="color: #00ff00"
     primer_bind     complement(4344..4388)
                     /label=BstZ17I_rp_68
                     /note="color: red; sequence: 
                     AGATAAAGGTTCTCCTCAATTTGTATACCCAGTTCAGGCCCTCAG; added: 
                     2021-02-07"
     misc_feature    4803..4813
                     /label=Transcription stop/pA signal (M)
                     /note="color: #ffff00"
     misc_feature    4819..4827
                     /label=Transcription start signal
                     /note="color: #ffff00"
     mRNA            4819..4827
                     /product="G mRNA"
                     /label=G mRNA
                     /note="color: #ffc0cb; direction: RIGHT"
     misc_feature    4836
                     /label=SmaI/XmaI site
                     /label=SmaI/XmaI site(1)
                     /note="color: #0000ff"
     misc_feature    4837..4841
                     /label=SmaI/XmaI site
                     /note="color: #0000ff"
     misc_feature    4845..4853
                     /label=Kozak sequence
                     /note="color: #ffff80"
     misc_feature    4854..5909
                     /label=mCre
                     /note="color: #00ff00"
     misc_feature    5913
                     /label=NheI site
                     /note="color: #0000ff"
     misc_feature    5914..5918
                     /label=NheI site
                     /label=NheI site(1)
                     /note="color: #0000ff"
     misc_feature    5954..5962
                     /label=Transcriptional stop/polyadenylation signal
                     /note="color: #ffff00"
     misc_feature    5954..5962
                     /label=pseudogene-like sequence
                     /note="pseudogene-like sequence"
                     /note="color: #ffff80"
     mRNA            5954..5962
                     /product="G mRNA"
                     /label=G mRNA
                     /label=G mRNA(1)
                     /note="color: #ffc0cb; direction: RIGHT"
     misc_feature    5987..5995
                     /label=Transcriptional start (L)
                     /note="color: #ffff00"
     misc_feature    6017..12400
                     /label=L
                     /note="L"
                     /note="color: #00ff00"
     misc_feature    12453..12461
                     /label=Transcription stop/polyA signal (L)
                     /note="color: #ffff00"
     misc_feature    12462..12531
                     /label=SAD B19 trailer
                     /note="color: #ff80ff"
     misc_feature    12532..12617
                     /label=HdVRibo
                     /note="HdVRibo"
                     /note="color: #800080"
     misc_feature    12686..12733
                     /label=T7term
                     /note="T7term"
                     /note="color: #ff8000"
     misc_feature    complement(12807..12826)
                     /label=T3
                     /note="T3"
                     /note="color: #0000ff; direction: LEFT"
     primer_bind     complement(12904..12922)
                     /label=pEX_rsp_55
                     /note="color: black; sequence: CACTTTATGCTTCCGGCTC; added: 
                     2021-03-08"
     primer_bind     complement(12906..12931)
                     /label=pEX-Rev
                     /note="reverse sequencing primer"
                     /note="color: black; sequence: CAGGCTTTACACTTTATGCTTCCGGC; 
                     added: 2021-03-08"
     misc_feature    complement(14009..14869)
                     /label=Ampicillin
                     /note="Ampicillin"
                     /note="color: #0000ff; direction: LEFT"
ORIGIN
        1 ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac
       61 cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc
      121 cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt
      181 tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg
      241 gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag
      301 tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt
      361 ataagggatt ttgccgattt cggcctattg gttaaaaaaa tgagctgatt taacaaaaat
      421 ttaacgcgaa ttttaacaaa atattaacgc ttacaatttc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgagc gcgcgataag cttgatatcg aattcctgca gcgcgttgac
      661 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      721 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      781 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      841 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      901 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      961 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
     1021 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
     1081 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
     1141 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
     1201 gcggtaggcg tgtacggtgg gaggtctata taagcagagc tctctggcta actagagaac
     1261 ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc caagctggct
     1321 agattaagcg tctgatgagt ccgtgaggac gaaacccggc gtaccgggtc acgcttaaca
     1381 accagatcaa agaaaaaaca gacattgtca attgcaaagc aaaaatgtaa cacccctaca
     1441 atggatgccg acaagattgt attcaaagtc aataatcagg tggtctcttt gaagcctgag
     1501 attatcgtgg atcaatatga gtacaagtac cctgccatca aagatttgaa aaagccctgt
     1561 ataaccctag gaaaggctcc cgatttaaat aaagcataca agtcagtttt gtcaggcatg
     1621 agcgccgcca aacttaatcc tgacgatgta tgttcctatt tggcagcggc aatgcagttt
     1681 tttgagggga catgtccgga agactggacc agctatggaa ttgtgattgc acgaaaagga
     1741 gataagatca ccccaggttc tctggtggag ataaaacgta ctgatgtaga agggaattgg
     1801 gctctgacag gaggcatgga actgacaaga gaccccactg tccctgagca tgcgtcctta
     1861 gtcggtcttc tcttgagtct gtataggttg agcaaaatat ccgggcaaaa cactggtaac
     1921 tataagacaa acattgcaga caggatagag cagatttttg agacagcccc ttttgttaaa
     1981 atcgtggaac accatactct aatgacaact cacaaaatgt gtgctaattg gagtactata
     2041 ccaaacttca gatttttggc cggaacctat gacatgtttt tctcccggat tgagcatcta
     2101 tattcagcaa tcagagtggg cacagttgtc actgcttatg aagactgttc aggactggta
     2161 tcatttactg ggttcataaa acaaatcaat ctcaccgcta gagaggcaat actatatttc
     2221 ttccacaaga actttgagga agagataaga agaatgtttg agccagggca ggagacagct
     2281 gttcctcact cttatttcat ccacttccgt tcactaggct tgagtgggaa atctccttat
     2341 tcatcaaatg ctgttggtca cgtgttcaat ctcattcact ttgtaggatg ctatatgggt
     2401 caagtcagat ccctaaatgc aacggttatt gctgcatgtg ctcctcatga aatgtctgtt
     2461 ctagggggct atctgggaga ggaattcttc gggaaaggga catttgaaag aagattcttc
     2521 agagatgaga aagaacttca agaatacgag gcggctgaac tgacaaagac tgacgtagca
     2581 ctggcagatg atggaactgt caactctgac gacgaggact acttttcagg tgaaaccaga
     2641 agtccggagg ctgtttatac tcgaatcatg atgaatggag gtcgactaaa gagatctcac
     2701 atacggagat atgtctcagt cagttccaat catcaagccc gtccaaactc attcgccgag
     2761 tttctaaaca agacatatag tagtgacagt ggttccggcg agaacctcta cttccaaagt
     2821 ggctccggta gccatggctt cccgccggag gtggaggagc aggatgatgg cacgctgccc
     2881 atgtcttgtg cccaggagag cgggatggac cgtcaccctg cagcctgtgc ttctgctagg
     2941 atcaatgtgt aagaagttga ataacaaaat gccggaaatc tacggattgt gtatatccat
     3001 catgaaaaaa actaacaccc ctcctttcga accatcccaa acatgagcaa gatctttgtc
     3061 aatcctagtg ctattagagc cggtctggcc gatcttgaga tggctgaaga aactgttgat
     3121 ctgatcaata gaaatatcga agacaatcag gctcatctcc aaggggaacc catagaggtg
     3181 gacaatctcc ctgaggatat ggggcgactt cacctggatg atggaaaatc gcccaaccat
     3241 ggtgagatag ccaaggtggg agaaggcaag tatcgagagg actttcagat ggatgaagga
     3301 gaggatccta gcttcctgtt ccagtcatac ctggaaaatg ttggagtcca aatagtcaga
     3361 caaatgaggt caggagagag atttctcaag atatggtcac agaccgtaga agagattata
     3421 tcctatgtcg cggtcaactt tcccaaccct ccaggaaagt cttcagagga taaatcaacc
     3481 cagactactg gccgagagct caagaaggag acaacaccca ctccttctca gagagaaagc
     3541 caatcatcga aagccaggat ggcggctcaa attgcttctg gccctccagc ccttgaatgg
     3601 tcggctacca atgaagagga tgatctatca gtggaggctg agatcgctca ccagattgca
     3661 gaaagtttct ccaaaaaata taagtttccc tctcgatcct cagggatact cttgtataat
     3721 tttgagcaat tgaaaatgaa ccttgatgat atagttaaag aggcaaaaaa tgtaccaggt
     3781 gtgacccgtt tagcccatga cgggtccaaa ctccccctaa gatgtgtact gggatgggtc
     3841 gctttggcca actctaagaa attccagttg ttagtcgaat ccgacaagct gagtaaaatc
     3901 atgcaagatg acttgaatcg ctatacatct tgctaaccga acctctcccc tcagtccctc
     3961 tagacaataa aatccgagat gtcccaaagt caacatgaaa aaaacaggca acaccactga
     4021 taaaatgaac ctcctacgta agatagtgaa aaaccgcagg gacgaggaca ctcaaaaatc
     4081 ctctcccgcg tcagcccctc tggatgacga tgacttgtgg cttccacccc ctgaatacgt
     4141 cccgctgaaa gaacttacag gcaagaagaa catgaggaac ttttgtatca acggaagggt
     4201 taaagtgtgt agcccgaatg gttactcgtt caggatcctg cggcacattc tgaaatcatt
     4261 cgacgagata tattctggga atcataggat gatcgggtta gtcaaagtgg ttattggact
     4321 ggctttgtca ggatctccag tccctgaggg cctgaactgg gtatacaaat tgaggagaac
     4381 ctttatcttc cagtgggctg attccagggg ccctcttgaa ggggaggagt tggaatactc
     4441 tcaggagatc acttgggatg atgatactga gttcgtcgga ttgcaaataa gagtgattgc
     4501 aaaacagtgt catatccagg gcagagtctg gtgtatcaac atgaacccga gagcatgtca
     4561 actatggtct gacatgtctc ttcagacaca aaggtccgaa gaggacaaag attcctctct
     4621 gcttctagaa taatcagatt atatcccgca aatttatcac ttgtttacct ctggaggaga
     4681 gaacatatgg gctcaactcc aacccttggg agcaatataa caaaaaacat gttatggtgc
     4741 cattaaaccg ctgcatttca tcaaagtcaa gttgattacc tttacatttt gatcctcttg
     4801 gatgtgaaaa aaactattaa catccctcaa aagaccccgg gaaagccgcc accatggtgc
     4861 ccaagaagaa gaggaaggtg agcaacctgc tgaccgtgca ccagaacctg cccgccctgc
     4921 ccgtggacgc caccagcgac gaggtgcgca agaacctgat ggacatgttc cgcgaccgcc
     4981 aggccttcag cgagcacacc tggaagatgc tgctgagcgt gtgccgcagc tgggccgcct
     5041 ggtgcaagct gaacaaccgc aagtggttcc ccgccgagcc cgaggacgtg cgcgactacc
     5101 tgctgtacct gcaggcccgc ggcctggccg tgaagaccat ccagcagcac ctgggccagc
     5161 tgaacatgct gcaccgccgc agcggcctgc cccgccccag cgacagcaac gccgtgagcc
     5221 tggtgatgcg ccgcatccgc aaggagaacg tggacgccgg cgagcgcgcc aagcaggccc
     5281 tggccttcga gcgcaccgac ttcgaccagg tgcgcagcct gatggagaac agcgaccgct
     5341 gccaggacat ccgcaacctg gccttcctgg gcatcgccta caacaccctg ctgcgcatcg
     5401 ccgagatcgc ccgcatccgc gtgaaggaca tcagccgcac cgacggcggc cgcatgctga
     5461 tccacatcgg ccgcaccaag accctggtga gcaccgccgg cgtggagaag gccctgagcc
     5521 tgggcgtgac caagctggtg gagcgctgga tcagcgtgag cggcgtggcc gacgacccca
     5581 acaactacct gttctgccgc gtgcgcaaga acggcgtggc cgcccccagc gccaccagcc
     5641 agctgagcac ccgggccctg gagggcatct tcgaggccac ccaccgcctg atctacggcg
     5701 ccaaggacga cagcggccag cgctacctgg cctggagcgg ccacagcgcc cgcgtgggcg
     5761 ccgcccgcga catggcccgc gccggcgtga gcatccccga gatcatgcag gccggcggct
     5821 ggaccaacgt gaacatcgtg atgaactaca tccgcaacct ggacagcgag accggcgcca
     5881 tggtgcgcct gctggaggac ggcgactgat gagctagctt ataaagtgct gggtcatcta
     5941 agcttttcag tcgagaaaaa aacattagat cagaagaaca actggcaaca cttctcaacc
     6001 tgagacttac ttcaagatgc tcgatcctgg agaggtctat gatgacccta ttgacccaat
     6061 cgagttagag gctgaaccca gaggaacccc cattgtcccc aacatcttga ggaactctga
     6121 ctacaatctc aactctcctt tgatagaaga tcctgctaga ctaatgttag aatggttaaa
     6181 aacagggaat agaccttatc ggatgactct aacagacaat tgctccaggt ctttcagagt
     6241 tttgaaagat tatttcaaga aggtagattt gggttctctc aaggtgggcg gaatggctgc
     6301 acagtcaatg atttctctct ggttatatgg tgcccactct gaatccaaca ggagccggag
     6361 atgtataaca gacttggccc atttctattc caagtcgtcc cccatagaga agctgttgaa
     6421 tctcacgcta ggaaatagag ggctgagaat ccccccagag ggagtgttaa gttgccttga
     6481 gagggttgat tatgataatg catttggaag gtatcttgcc aacacgtatt cctcttactt
     6541 gttcttccat gtaatcacct tatacatgaa cgccctagac tgggatgaag aaaagaccat
     6601 cctagcatta tggaaagatt taacctcagt ggacatcggg aaggacttgg taaagttcaa
     6661 agaccaaata tggggactgc tgatcgtgac aaaggacttt gtttactccc aaagttccaa
     6721 ttgtcttttt gacagaaact acacacttat gctaaaagat cttttcttgt ctcgcttcaa
     6781 ctccttaatg gtcttgctct ctcccccaga gccccgatac tcagatgact tgatatctca
     6841 actatgccag ctgtacattg ctggggatca agtcttgtct atgtgtggaa actccggcta
     6901 tgaagtcatc aaaatattgg agccatatgt cgtgaatagt ttagtccaga gagcagaaaa
     6961 gtttaggcct ctcattcatt ccttgggaga ctttcctgta tttataaaag acaaggtaag
     7021 tcaacttgaa gagacgttcg gtccctgtgc aagaaggttc tttagggctc tggatcaatt
     7081 cgacaacata catgacttgg tttttgtgtt tggctgttac aggcattggg ggcacccata
     7141 tatagattat cgaaagggtc tgtcaaaact atatgatcag gttcacctta aaaaaatgat
     7201 agataagtcc taccaggagt gcttagcaag cgacctagcc aggaggatcc ttagatgggg
     7261 ttttgataag tactccaagt ggtatctgga ttcaagattc ctagcccgag accacccctt
     7321 gactccttat atcaaaaccc aaacatggcc acccaaacat attgtagact tggtggggga
     7381 tacatggcac aagctcccga tcacgcagat ctttgagatt cctgaatcaa tggatccgtc
     7441 agaaatattg gatgacaaat cacattcttt caccagaacg agactagctt cttggctgtc
     7501 agaaaaccga ggggggcctg ttcctagcga aaaagttatt atcacggccc tgtctaagcc
     7561 gcctgtcaat ccccgagagt ttctgaggtc tatagacctc ggaggattgc cagatgaaga
     7621 cttgataatt ggcctcaagc caaaggaacg ggaattgaag attgaaggtc gattctttgc
     7681 tctaatgtca tggaatctaa gattgtattt tgtcatcact gaaaaactct tggccaacta
     7741 catcttgcca ctttttgacg cgctgactat gacagacaac ctgaacaagg tgtttaaaaa
     7801 gctgatcgac agggtcaccg ggcaagggct tttggactat tcaagggtca catatgcatt
     7861 tcacctggac tatgaaaagt ggaacaacca tcaaagatta gagtcaacag aggatgtatt
     7921 ttctgtccta gatcaagtgt ttggattgaa gagagtgttt tctagaacac acgagttttt
     7981 tcaaaaggcc tggatctatt attcagacag atcagacctc atcgggttac gggaggatca
     8041 aatatactgc ttagatgcgt ccaacggccc aacctgttgg aatggccagg atggcgggct
     8101 agaaggctta cggcagaagg gctggagtct agtcagctta ttgatgatag atagagaatc
     8161 tcaaatcagg aacacaagaa ccaaaatact agctcaagga gacaaccagg ttttatgtcc
     8221 gacatacatg ttgtcgccag ggctatctca agaggggctc ctctatgaat tggagagaat
     8281 atcaaggaat gcactttcga tatacagagc cgtcgaggaa ggggcatcta agctagggct
     8341 gatcatcaag aaagaagaga ccatgtgtag ttatgacttc ctcatctatg gaaaaacccc
     8401 tttgtttaga ggtaacatat tggtgcctga gtccaaaaga tgggccagag tctcttgcgt
     8461 ctctaatgac caaatagtca acctcgccaa tataatgtcg acagtgtcca ccaatgcgct
     8521 aacagtggca caacactctc aatctttgat caaaccgatg agggattttc tgctcatgtc
     8581 agtacaggca gtctttcact acctgctatt tagcccaatc ttaaagggaa gagtttacaa
     8641 gattctgagc gctgaagggg agagctttct cctagccatg tcaaggataa tctatctaga
     8701 tccttctttg ggagggatat ctggaatgtc cctcggaaga ttccatatac gacagttctc
     8761 agaccctgtc tctgaagggt tatccttctg gagagagatc tggttaagct cccaagagtc
     8821 ctggattcac gcgttgtgtc aagaggctgg aaacccagat cttggagaga gaacactcga
     8881 gagcttcact cgccttctag aagatccgac caccttaaat atcagaggag gggccagtcc
     8941 taccattcta ctcaaggatg caatcagaaa ggctttatat gacgaggtgg acaaggtgga
     9001 aaattcagag tttcgagagg caatcctgtt gtccaagacc catagagata attttatact
     9061 cttcttaata tctgttgagc ctctgtttcc tcgatttctc agtgagctat tcagttcgtc
     9121 ttttttggga atccccgagt caatcattgg attgatacaa aactcccgaa cgataagaag
     9181 gcagtttaga aagagtctct caaaaacttt agaagaatcc ttctacaact cagagatcca
     9241 cgggattagt cggatgaccc agacacctca gagggttggg ggggtgtggc cttgctcttc
     9301 agagagggca gatctactta gggagatctc ttggggaaga aaagtggtag gcacgacagt
     9361 tcctcaccct tctgagatgt tgggattact tcccaagtcc tctatttctt gcacttgtgg
     9421 agcaacagga ggaggcaatc ctagagtttc tgtatcagta ctcccgtcct ttgatcagtc
     9481 atttttttca cgaggccccc taaagggata cttgggctcg tccacctcta tgtcgaccca
     9541 gctattccat gcatgggaaa aagtcactaa tgttcatgtg gtgaagagag ctctatcgtt
     9601 aaaagaatct ataaactggt tcattactag agattccaac ttggctcaag ctctaattag
     9661 gaacattatg tctctgacag gccctgattt ccctctagag gaggcccctg tcttcaaaag
     9721 gacggggtca gccttgcata ggttcaagtc tgccagatac agcgaaggag ggtattcttc
     9781 tgtctgcccg aacctcctct ctcatatttc tgttagtaca gacaccatgt ctgatttgac
     9841 ccaagacggg aagaactacg atttcatgtt ccagccattg atgctttatg cacagacatg
     9901 gacatcagag ctggtacaga gagacacaag gctaagagac tctacgtttc attggcacct
     9961 ccgatgcaac aggtgtgtga gacccattga cgacgtgacc ctggagacct ctcagatctt
    10021 cgagtttccg gatgtgtcga aaagaatatc cagaatggtt tctggggctg tgcctcactt
    10081 ccagaggctt cccgatatcc gtctgagacc aggagatttt gaatctctaa gcggtagaga
    10141 aaagtctcac catatcggat cagctcaggg gctcttatac tcaatcttag tggcaattca
    10201 cgactcagga tacaatgatg gaaccatctt ccctgtcaac atatacggca aggtttcccc
    10261 tagagactat ttgagagggc tcgcaagggg agtattgata ggatcctcga tttgcttctt
    10321 gacaagaatg acaaatatca atattaatag acctcttgaa ttggtctcag gggtaatctc
    10381 atatattctc ctgaggctag ataaccatcc ctccttgtac ataatgctca gagaaccgtc
    10441 tcttagagga gagatatttt ctatccctca gaaaatcccc gccgcttatc caaccactat
    10501 gaaagaaggc aacagatcaa tcttgtgtta tctccaacat gtgctacgct atgagcgaga
    10561 gataatcacg gcgtctccag agaatgactg gctatggatc ttttcagact ttagaagtgc
    10621 caaaatgacg tacctatccc tcattactta ccagtctcat cttctactcc agagggttga
    10681 gagaaaccta tctaagagta tgagagataa cctgcgacaa ttgagttctt tgatgaggca
    10741 ggtgctgggc gggcacggag aagatacctt agagtcagac gacaacattc aacgactgct
    10801 aaaagactct ttacgaagga caagatgggt ggatcaagag gtgcgccatg cagctagaac
    10861 catgactgga gattacagcc ccaacaagaa ggtgtcccgt aaggtaggat gttcagaatg
    10921 ggtctgctct gctcaacagg ttgcagtctc tacctcagca aacccggccc ctgtctcgga
    10981 gcttgacata agggccctct ctaagaggtt ccagaaccct ttgatctcgg gcttgagagt
    11041 ggttcagtgg gcaaccggtg ctcattataa gcttaagcct attctagatg atctcaatgt
    11101 tttcccatct ctctgccttg tagttgggga cgggtcaggg gggatatcaa gggcagtcct
    11161 caacatgttt ccagatgcca agcttgtgtt caacagtctt ttagaggtga atgacctgat
    11221 ggcttccgga acacatccac tgcctccttc agcaatcatg aggggaggaa atgatatcgt
    11281 ctccagagtg atagatcttg actcaatctg ggaaaaaccg tccgacttga gaaacttggc
    11341 aacctggaaa tacttccagt cagtccaaaa gcaggtcaac atgtcctatg acctcattat
    11401 ttgcgatgca gaagttactg acattgcatc tatcaaccgg atcaccctgt taatgtccga
    11461 ttttgcattg tctatagatg gaccactcta tttggtcttc aaaacttatg ggactatgct
    11521 agtaaatcca aactacaagg ctattcaaca cctgtcaaga gcgttcccct cggtcacagg
    11581 gtttatcacc caagtaactt cgtctttttc atctgagctc tacctccgat tctccaaacg
    11641 agggaagttt ttcagagatg ctgagtactt gacctcttcc acccttcgag aaatgagcct
    11701 tgtgttattc aattgtagca gccccaagag tgagatgcag agagctcgtt ccttgaacta
    11761 tcaggatctt gtgagaggat ttcctgaaga aatcatatca aatccttaca atgagatgat
    11821 cataactctg attgacagtg atgtagaatc ttttctagtc cacaagatgg ttgatgatct
    11881 tgagttacag aggggaactc tgtctaaagt ggctatcatt atagccatca tgatagtttt
    11941 ctccaacaga gtcttcaacg tttccaaacc cctaactgac ccctcgttct atccaccgtc
    12001 tgatcccaaa atcctgaggc acttcaacat atgttgcagt actatgatgt atctatctac
    12061 tgctttaggt gacgtcccta gcttcgcaag acttcacgac ctgtataaca gacctataac
    12121 ttattacttc agaaagcaag tcattcgagg gaacgtttat ctatcttgga gttggtccaa
    12181 cgacacctca gtgttcaaaa gggtagcctg taattctagc ctgagtctgt catctcactg
    12241 gatcaggttg atttacaaga tagtgaagac taccagactc gttggcagca tcaaggatct
    12301 atccagagaa gtggaaagac accttcatag gtacaacagg tggatcaccc tagaggatat
    12361 cagatctaga tcatccctac tagactacag ttgcctgtga accggatact cctggaagcc
    12421 tgcccatgct aagactcttg tgtgatgtat cttgaaaaaa acaagatcct aaatctgaac
    12481 ctttggttgt ttgattgttt ttctcatttt tgttgtttat ttgttaagcg tgggtcggca
    12541 tggcatctcc acctcctcgc ggtccgacct gggcatccga aggaggacgc acgtccactc
    12601 ggatggctaa gggaggggcg gggatccggc tgctaacaaa gcccgaaagg aagctgagtt
    12661 ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt
    12721 gaggggtttt ttgctgaaag gaggaactat atccggatcc actagttcta gagcggccgc
    12781 caccgcggtg gagctccagc ttttgttccc tttagtgagg gttaattgcg cgcttggcgt
    12841 aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca
    12901 tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat
    12961 taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt
    13021 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct
    13081 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
    13141 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
    13201 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
    13261 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
    13321 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
    13381 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
    13441 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
    13501 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
    13561 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
    13621 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
    13681 acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
    13741 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
    13801 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
    13861 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
    13921 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
    13981 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
    14041 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
    14101 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct
    14161 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
    14221 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
    14281 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt
    14341 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
    14401 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
    14461 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
    14521 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
    14581 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg
    14641 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
    14701 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
    14761 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
    14821 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
    14881 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
    14941 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg
//
