LOCUS       Exported                5878 bp ds-DNA     circular SYN 23-JUL-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5878)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Jul 23, 2021 from SnapGene Viewer 5.2.4
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5878
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        90..616
                     /label=CMV promoter
     primer_bind     828..845
                     /label=SP6
     misc_feature    931..1341
                     /label=Vamp4
     misc_feature    1378..2088
                     /label=SEP
     misc_feature    2551..2628
                     /label=SV40 ORI
     primer_bind     complement(2720..2737)
                     /label=M13-fwd
     rep_origin      2949..3404
                     /direction=RIGHT
                     /label=M13 origin
     rep_origin      complement(2954..3394)
                     /direction=LEFT
                     /label=F1 ori
     promoter        3771..3799
                     /label=Amp prom
     promoter        5784..5813
                     /label=lac
     misc_binding    5818..5840
                     /label=LacO
     primer_bind     5846..5866
                     /label=M13-rev
ORIGIN
        1 ttcgagctcg cccgacattg attattgact agttattaat agtaatcaat tacggggtca
       61 ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct
      121 ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta
      181 acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac
      241 ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt
      301 aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag
      361 tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat
      421 gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat
      481 gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc
      541 ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctcgt
      601 ttagtgaacc gtcagatcgc ctggagacgc catccacgct gttttgacct ccatagaaga
      661 caccgggacc gatccagcct ccgcggccgg gaacggtgca ttggaacgcg gattccccgt
      721 gccaagagtg acgtaactag cgcctataga gtctataggc ccaccccctt ggcttcgtta
      781 gaacgcggct acaattaata cataacctta tgtatcatac acatacgatt taggtgacac
      841 tatagaataa catccacttt gcctttctct ccacaggtgt ccactcccag gtccaactgc
      901 acctcggttc tatcgattga attcgccacc atgcctccca agtttaagcg ccacctcaat
      961 gatgatgatg tcacaggttc tgtgaaaagt gaaaggagaa atcttttgga agatgattca
     1021 gatgaagaag aggacttttt tctaagggga ccatctggac caagatttgg acctagaaat
     1081 gataaaatta agcatgttca gaatcaagtg gatgaagtta ttgatgtcat gcaagaaaat
     1141 attacaaagg taattgagag aggggagaga ctagatgaac tacaggacaa atcagaaagc
     1201 ttatcggata atgcaacagc ttttagcaac agatccaaac aacttcgaag gcaaatgtgg
     1261 tggcgtggat gcaaaataaa agccatcatg gctttggttg ctgctatcct tttgctagtg
     1321 attatcattc ttatagtcat gaaataccgt acttcggatc caccggtcgc caccatgagt
     1381 aaaggagaag aacttttcac tggagttgtc ccaattcttg ttgaattaga tggtgatgtt
     1441 aatgggcaca aattttctgt cagtggagag ggtgaaggtg atgcaacata cggaaaactt
     1501 acccttaaat ttatttgcac tactggaaaa ctacctgttc catggccaac acttgtcact
     1561 actttaactt atggtgttca atgcttttca agatacccag atcatatgaa acggcatgac
     1621 tttttcaaga gtgccatgcc cgaaggttat gtacaggaaa gaactatatt tttcaaagat
     1681 gacgggaact acaagacacg tgctgaagtc aagtttgaag gtgataccct tgttaataga
     1741 atcgagttaa aaggtattga ttttaaagaa gatggaaaca ttcttggaca caaattggaa
     1801 tacaactata acgatcacca ggtgtacatc atggcagaca aacaaaagaa tggaatcaaa
     1861 gctaacttca aaattagaca caacattgaa gatggaggcg ttcaactagc agaccattat
     1921 caacaaaata ctccaattgg cgatgggccc gtccttttac cagacaacca ttacctgttt
     1981 acaacttcta ctctttcgaa agatcccaac gaaaagagag accacatggt ccttcttgag
     2041 tttgtaacag ctgctgggat tacacatggc atggatgaac tatacaaagc tagctaatct
     2101 agagtcgacc tgcagaagct tggccgccat ggcccaactt gtttattgca gcttataatg
     2161 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
     2221 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggatc gatcgggaat
     2281 taattcggcg cagcaccatg gcctgaaata acctctgaaa gaggaacttg gttaggtacc
     2341 ttctgaggcg gaaagaacca gctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca
     2401 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt
     2461 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatgt caattagtca
     2521 gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc
     2581 cattctccgc cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg
     2641 gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa
     2701 aagctgttaa cagcttggca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg
     2761 gcgttaccca acttaatcgc cttgcagcac atcccccctt cgccagctgg cgtaatagcg
     2821 aagaggcccg caccgatcgc ccttcccaac agttgcgtag cctgaatggc gaatggcgcc
     2881 tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata cgtcaaagca
     2941 accatagtac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag
     3001 cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt
     3061 tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt
     3121 ccgatttagt gctttacggc acctcgaccc caaaaaactt gatttgggtg atggttcacg
     3181 tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt
     3241 taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg gctattcttt
     3301 tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca
     3361 aaaatttaac gcgaatttta acaaaatatt aacgtttaca attttatggt gcactctcag
     3421 tacaatctgc tctgatgccg catagttaag ccaactccgc tatcgctacg tgactgggtc
     3481 atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc
     3541 ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt
     3601 tcaccgtcat cacccaaacg cgcgaggcag tattcttgaa gacgaaaggg cctcgtgata
     3661 cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact
     3721 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg
     3781 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt
     3841 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct
     3901 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca
     3961 cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc
     4021 gaagaacgtt ttccaatgct gcgcactttt aaagttctgc tatgtggcgc ggtattatcc
     4081 cgtgatgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg
     4141 gttgactact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta
     4201 tgcagtgctg ccataaccat gagtcataac actgcggcca acttacttct gacaacgatc
     4261 ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt
     4321 gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg
     4381 ccagcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct
     4441 tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc
     4501 tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct
     4561 cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac
     4621 acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc
     4681 tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat
     4741 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg
     4801 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
     4861 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     4921 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     4981 gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta
     5041 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatccgtgta
     5101 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     5161 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     5221 gagcgaacga cctacaccga actgagatac ctacagcgtg agcattgaga aagcgccacg
     5281 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     5341 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     5401 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     5461 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
     5521 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
     5581 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
     5641 gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atccagctgg
     5701 cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttac
     5761 ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga
     5821 attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta cgaattaa
//
