LOCUS       Exported               10158 bp ds-DNA     circular SYN 23-JUL-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10158)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Jul 23, 2021 from SnapGene Viewer 5.2.4
            https://www.snapgene.com
COMMENT     Ligation of : DC1770-mscarlet.txt.xdna [9873  nt] : (XhoI[2839] / 
            EcoRI[2604]) to 850-DC_853___pSUPER_shVamp4_1.xdna [277  nt] : 
            (EcoRI[707] / XhoI[988])
FEATURES             Location/Qualifiers
     source          1..10158
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    749..766
                     /label=pCMV f
     LTR             835..1015
                     /label=HIV-1 5 LTR
     misc_feature    1126..1170
                     /label=HIV-1 psi pack
     misc_feature    1680..1913
                     /label=RRE
                     2609..2885
                     /note="note=""Fragment extracted from 
                     850-DC_853___pSUPER_shVamp4_1.xdna [277  nt] : (EcoRI[707] 
                     / XhoI[988])"
     misc_feature    2735..2825
                     /label=Promoteur H1
     misc_feature    2735..2825
                     /label=H1 promotor
     misc_feature    2826..2885
                     /label=shVamp4-1
     misc_feature    2900..2907
                     /label=PacI site
     misc_feature    2925..4077
                     /label=Promoteur Ubc
                     4107..4819
                     /note="note=""Fragment extracted from 
                     Minigene_mScarlett_modifiee SalI.txt.xdna [713  nt] : 
                     (XbaI[3] / SalI[720])"
     misc_feature    4114..4703
                     /label=mScralet1 [*]
                     /note="note=""direct strand 
                     translation""translation=""VSKGEAVIKEFMRFKVHMEGSMNGHEFEIEGE
                     GEGRPYEGTQTAKLKVTKGGPLPFSWDILSPQFMYGSRAFIKHPADIPDYYKQSFPEGF
                     KWERVMNFEDGGAVTVTQDTSLEDGTLIYKVKLRGTNFPPDGPVMQKKTMGWEASTERL
                     YPEDGVLKGDIKMALRLKDGGRYLADFKTTYKAKKPVQMPGAYNVDRKLDITSHNEDYT
                     V"
     misc_feature    4704
                     /label=mutation SalI inactif
                     /note="note=""direct strand 
                     translation""translation=""VSKGEAVIKEFMRFKVHMEGSMNGHEFEIEGE
                     GEGRPYEGTQTAKLKVTKGGPLPFSWDILSPQFMYGSRAFIKHPADIPDYYKQSFPEGF
                     KWERVMNFEDGGAVTVTQDTSLEDGTLIYKVKLRGTNFPPDGPVMQKKTMGWEASTERL
                     YPEDGVLKGDIKMALRLKDGGRYLADFKTTYKAKKPVQMPGAYNVDRKLDITSHNEDYT
                     V"
     misc_feature    4705..4813
                     /label=mscarlet
     CDS             complement(5327..5338)
                     /label=FactorXa site
     LTR             5963..6143
                     /label=HIV-1 5 LTR
     misc_feature    complement(6169..6186)
                     /label=Bgh r
     terminator      6172..6399
                     /label=bGH PA
     misc_feature    7067..7144
                     /label=SV40 ORI
     promoter        7263..7329
                     /label=EM7 promoter
     promoter        7263..7329
                     /label=mPGK Prom
     CDS             7330..7704
                     /label=ZeoR
     misc_feature    complement(7994..8015)
                     /label=M13R
     primer_bind     complement(8000..8020)
                     /label=M13-rev
     misc_feature    complement(8004..8020)
                     /label=LMB3
     misc_binding    complement(8026..8048)
                     /label=LacO
     promoter        complement(8053..8082)
                     /label=lac
     rep_origin      complement(8388..9016)
                     /direction=LEFT
                     /label=ColE1 origin
     CDS             complement(9168..9827)
                     /label=AmpR
     promoter        complement(10067..10095)
                     /label=Amp prom
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
     2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     2581 agatccagtt tggttaatcg tacgaattcg aacgctgacg tcatcaaccc gctccaagga
     2641 atcgcgggcc cagtgtcact aggcgggaac acccagcgcg cgtgcgccct ggcaggaaga
     2701 tggctgtgag ggacagggga gtggcgccct gcaatatttg catgtcgcta tgtgttctgg
     2761 gaaatcacca taaacgtgaa atgtctttgg atttgggaat cttataagtt ctgtatgaga
     2821 ccacagatcc ccctatcttt atttaacaac attcaagaga tgttgttaaa taaagatagt
     2881 ttttctcgag cttatcgaat taattaaggg tgcagcggcc tccgcgccgg gttttggcgc
     2941 ctcccgcggg cgcccccctc ctcacggcga gcgctgccac gtcagacgaa gggcgcagga
     3001 gcgttcctga tccttccgcc cggacgctca ggacagcggc ccgctgctca taagactcgg
     3061 ccttagaacc ccagtatcag cagaaggaca ttttaggacg ggacttgggt gactctaggg
     3121 cactggtttt ctttccagag agcggaacag gcgaggaaaa gtagtccctt ctcggcgatt
     3181 ctgcggaggg atctccgtgg ggcggtgaac gccgatgatt atataaggac gcgccgggtg
     3241 tggcacagct agttccgtcg cagccgggat ttgggtcgcg gttcttgttt gtggatcgct
     3301 gtgatcgtca cttggtgagt tgcgggctgc tgggctggcc ggggctttcg tggccgccgg
     3361 gccgctcggt gggacggaag cgtgtggaga gaccgccaag ggctgtagtc tgggtccgcg
     3421 agcaaggttg ccctgaactg ggggttgggg ggagcgcaca aaatggcggc tgttcccgag
     3481 tcttgaatgg aagacgcttg taaggcgggc tgtgaggtcg ttgaaacaag gtggggggca
     3541 tggtgggcgg caagaaccca aggtcttgag gccttcgcta atgcgggaaa gctcttattc
     3601 gggtgagatg ggctggggca ccatctgggg accctgacgt gaagtttgtc actgactgga
     3661 gaactcgggt ttgtcgtctg gttgcggggg cggcagttat gcggtgccgt tgggcagtgc
     3721 acccgtacct ttgggagcgc gcgcctcgtc gtgtcgtgac gtcacccgtt ctgttggctt
     3781 ataatgcagg gtggggccac ctgccggtag gtgtgcggta ggcttttctc cgtcgcagga
     3841 cgcagggttc gggcctaggg taggctctcc tgaatcgaca ggcgccggac ctctggtgag
     3901 gggagggata agtgaggcgt cagtttcttt ggtcggtttt atgtacctat cttcttaagt
     3961 agctgaagct ccggttttga actatgcgct cggggttggc gagtgtgttt tgtgaagttt
     4021 tttaggcacc ttttgaaatg taatcatttg ggtcaatatg taattttcag tgttagacta
     4081 gtaaagcttc tgcaggtcga ctctagagcc accatggtga gcaagggcga ggcagtgatc
     4141 aaggagttca tgcggttcaa ggtgcacatg gagggctcca tgaacggcca cgagttcgag
     4201 atcgagggcg agggcgaggg ccgcccctac gagggcaccc agaccgccaa gctgaaggtg
     4261 accaagggtg gccccctgcc cttctcctgg gacatcctgt cccctcagtt catgtacggc
     4321 tccagggcct tcatcaagca ccccgccgac atccccgact actataagca gtccttcccc
     4381 gagggcttca agtgggagcg cgtgatgaac ttcgaggacg gcggcgccgt gaccgtgacc
     4441 caggacacct ccctggagga cggcaccctg atctacaagg tgaagctccg cggcaccaac
     4501 ttccctcctg acggccccgt aatgcagaag aagacaatgg gctgggaagc gtccaccgag
     4561 cggttgtacc ccgaggacgg cgtgctgaag ggcgacatta agatggccct gcgcctgaag
     4621 gacggcggcc gctacctggc ggacttcaag accacctaca aggccaagaa gcccgtgcag
     4681 atgcccggcg cctacaacgt cgatcgcaag ttggacatca cctcccacaa cgaggactac
     4741 accgtggtgg aacagtacga acgctccgag ggccgccact ccaccggcgg catggacgag
     4801 ctgtacaagt aaaccggtgt cgagctagaa ttaattcgat atcaagctta tcgataatca
     4861 acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt
     4921 tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc
     4981 tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc
     5041 cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg
     5101 gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc
     5161 cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg
     5221 cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg
     5281 tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc
     5341 agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct
     5401 tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcatcgat accgtcgaga
     5461 cctagaaaaa catggagcaa tcacaagtag caatacagca gctaccaatg ctgattgtgc
     5521 ctggctagaa gcacaagagg aggaggaggt gggttttcca gtcacacctc aggtaccttt
     5581 aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagggggg
     5641 actggaaggg ctaattcact cccaacgaag acaagatatc cttgatctgt ggatctacca
     5701 cacacaaggc tacttccctg attggcagaa ctacacacca gggccaggga tcagatatcc
     5761 actgaccttt ggatggtgct acaagctagt accagttgag caagagaagg tagaagaagc
     5821 caatgaagga gagaacaccc gcttgttaca ccctgtgagc ctgcatggga tggatgaccc
     5881 ggagagagaa gtattagagt ggaggtttga cagccgccta gcatttcatc acatggcccg
     5941 agagctgcat ccggactgta ctgggtctct ctggttagac cagatctgag cctgggagct
     6001 ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca
     6061 agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta
     6121 gtcagtgtgg aaaatctcta gcagggcccg tttaaacccg ctgatcagcc tcgactgtgc
     6181 cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag
     6241 gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta
     6301 ggtgtcattc tattctgggg ggtggggtgg ggcaggacag caagggggag gattgggaag
     6361 acaatagcag gcatgctggg gatgcggtgg gctctatggc ttctgaggcg gaaagaacca
     6421 gctggggctc tagggggtat ccccacgcgc cctgtagcgg cgcattaagc gcggcgggtg
     6481 tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg
     6541 ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg
     6601 ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt
     6661 agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt
     6721 tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta
     6781 tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa
     6841 atgagctgat ttaacaaaaa tttaacgcga attaattctg tggaatgtgt gtcagttagg
     6901 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta
     6961 gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
     7021 gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac
     7081 tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga
     7141 ggccgaggcc gcctctgcct ctgagctatt ccagaagtag tgaggaggct tttttggagg
     7201 cctaggcttt tgcaaaaagc tcccgggagc ttgtatatcc attttcggat ctgatcagca
     7261 cgtgttgaca attaatcatc ggcatagtat atcggcatag tataatacga caaggtgagg
     7321 aactaaacca tggccaagtt gaccagtgcc gttccggtgc tcaccgcgcg cgacgtcgcc
     7381 ggagcggtcg agttctggac cgaccggctc gggttctccc gggacttcgt ggaggacgac
     7441 ttcgccggtg tggtccggga cgacgtgacc ctgttcatca gcgcggtcca ggaccaggtg
     7501 gtgccggaca acaccctggc ctgggtgtgg gtgcgcggcc tggacgagct gtacgccgag
     7561 tggtcggagg tcgtgtccac gaacttccgg gacgcctccg ggccggccat gaccgagatc
     7621 ggcgagcagc cgtgggggcg ggagttcgcc ctgcgcgacc cggccggcaa ctgcgtgcac
     7681 ttcgtggccg aggagcagga ctgacacgtg ctacgagatt tcgattccac cgccgccttc
     7741 tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg gctggatgat cctccagcgc
     7801 ggggatctca tgctggagtt cttcgcccac cccaacttgt ttattgcagc ttataatggt
     7861 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
     7921 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctgtatacc gtcgacctct
     7981 agctagagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc
     8041 acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga
     8101 gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg
     8161 tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg
     8221 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
     8281 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
     8341 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
     8401 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
     8461 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
     8521 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
     8581 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
     8641 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
     8701 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
     8761 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
     8821 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca
     8881 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
     8941 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
     9001 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
     9061 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
     9121 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
     9181 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
     9241 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
     9301 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
     9361 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
     9421 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
     9481 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
     9541 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
     9601 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
     9661 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
     9721 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
     9781 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
     9841 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
     9901 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
     9961 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
    10021 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
    10081 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
    10141 cgaaaagtgc cacctgac
//
