LOCUS       pCE41                   4321 bp ds-DNA     circular     25-JUL-2021
DEFINITION  .
FEATURES             Location/Qualifiers
     CDS             complement(75..932)
                     /label="bla (AmpR)"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    1129..1156
                     /label="5' Linker"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     misc_feature    1157..1657
                     /label="OVERLAP"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    1157..1918
                     /label="HygB"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    1157..1918
                     /label="HygB 2/2"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     CDS             1157..1918
                     /label="Translation 1157-1918"
     misc_feature    1919..2363
                     /label="pSNR52 - CE"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     misc_feature    2364..2383
                     /label="C. auris ADE2 gRNA"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     primer          complement(2384..2403)
                     /label="AHO1018"
                     /note="sequence: GCTATTTCTAGCTCTAAAAC"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     primer          2384..2408
                     /label="AHO1099"
                     /note="sequence: GTTTTAGAGCTAGAAATAGCAAGTT"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    2384..2472
                     /label="gRNA conserved"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     primer          2458..2485
                     /label="AHO1195"
                     /note="sequence: GCTTTTTTTCTCGAGtttttttaTCGAG"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     primer          complement(2458..2485)
                     /label="AHO1061"
                     /note="sequence: CTCGAtaaaaaaaCTCGAGAAAAAAAGC"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     primer          2466..2505
                     /label="AHO1930"
                     /note="sequence: TCTCGAGtttttttaTCGAGTCTCCATTGAAGGCCAGTGT"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    2486..3235
                     /label="C.auris LEU2"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     primer          2928..2945
                     /label="AHO1932"
                     /note="sequence: GTCTTTTACCTTCTGCAT"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     primer          complement(3211..3255)
                     /label="AHO1931"
                     /note="sequence: GCGCTGGGGTTTAAACACCGTTATTTCTGCAAAAGCTTCTTTACC"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     primer          3236..3253
                     /label="AHO1095"
                     /note="sequence: CGGTGTTTAAACCCCAGC"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    3236..3263
                     /label="3' Linker"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     primer          complement(3236..3263)
                     /label="AHO1097"
                     /note="sequence: CCCGCCAGGCGCTGGGGTTTAAACACCG"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     primer          3236..3288
                     /label="AHO1059"
                     /note="sequence: CGGTGTTTAAACCCCAGCGCCTGGCGGGcgctcacaattccacacaacatacg"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     primer          complement(3239..3256)
                     /label="AHO1199"
                     /note="sequence: GGCGCTGGGGTTTAAACA"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     primer          complement(3248..3263)
                     /label="AHO1134"
                     /note="sequence: CCCGCCAGGCGCTGGG"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     primer          3264..3287
                     /label="AHO1006"
                     /note="sequence: cgctcacaattccacacaacatac"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     primer          complement(3265..3287)
                     /label="AHO254"
                     /note="sequence: GTATGTTGTGTGGAATTGTGAGC"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     primer          complement(3358..3377)
                     /label="AHO582"
                     /note="sequence: ACTGGAAAGCGGGCAGTGAG"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     rep_origin      complement(3634..4222)
                     /label="pMB1\Ori"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
ORIGIN
        1 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
       61 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
      121 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
      181 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta
      241 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
      301 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
      361 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt
      421 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
      481 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
      541 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
      601 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
      661 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact
      721 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
      781 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
      841 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
      901 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc
      961 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
     1021 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta agaaaccatt
     1081 attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttGA CGGCACGGCC
     1141 ACGCGTTTAA ACCGCCTACT GTATAAGTCG AAGAGCACAA GGGGTTACTT TACAAGACTT
     1201 GCCAGAAACC GAATTACCAG CCGTGCTTCA GCCAGTAGCT GAGGCTATGG ACGCAATCGC
     1261 TGCAGCCGAT TTAAGTCAAA CATCAGGTTT CGGTCCTTTT GGGCCACAAG GGATCGGTCA
     1321 ATATACTACC TGGCGTGATT TTATTTGCGC CATAGCAGAT CCTCACGTGT ACCACTGGCA
     1381 AACCGTCATG GATGACACAG TCTCAGCATC CGTGGCCCAG GCACTTGACG AATTGATGTT
     1441 ATGGGCAGAG GATTGTCCTG AGGTGCGACA CTTGGTTCAT GCCGACTTTG GGAGTAACAA
     1501 CGTCTTAACA GACAACGGAC GAATAACCGC TGTGATTGAC TGGTCAGAAG CTATGTTCGG
     1561 TGACTCTCAA TACGAAGTTG CCAACATCTT CTTCTGGCGA CCTTGGCTTG CCTGCATGGA
     1621 ACAGCAAACA CGATACTTCG AACGACGACA TCCAGAACTT GCCGGATCTC CTCGATTACG
     1681 TGCCTATATG TTACGTATCG GTTTAGACCA ATTGTATCAG AGTTTGGTCG ACGGAAATTT
     1741 CGACGACGCC GCATGGGCAC AGGGTCGTTG TGACGCCATA GTTAGAAGTG GAGCAGGTAC
     1801 CGTTGGGAGA ACTCAGATTG CTCGTAGATC CGCAGCAGTA TGGACAGATG GATGTGTAGA
     1861 GGTTTTAGCC GACAGTGGAA ACCGAAGACC TTCAACAAGA CCACGTGCCA AGGAGTAAAG
     1921 CTTAACTTTT AGAGTCACTT ACCCTACAAA GGTGTAAGCT TCCCTCAGAT CAGGCGGCAC
     1981 GCATCTGATA TCTAACATCC ATTGTAAAAT TAGTGAAGAT TAACACTCAG AGCCAGTTAT
     2041 TTTGAACACA GATGGCTTTT GGCTGCTCTT ATCGTCTACG TTTCTTTTTT TTTTTTTTTT
     2101 TTTTTTTTTT TTTTTTTTCC TAATTTTTTA CGAGTTCACT AATTGGTTTC GGGGGCTCAA
     2161 TTTTCGAATG CGACATTCAT AAACTTTGAC CTTTTATGTG TTGATGCGGA TAATTTTGAC
     2221 CTCAAAACTC GGAAAAATTG ATGTATAGCG TATTCGACAT GGCTTTAAAA GTCGCAGAGT
     2281 TCTACGATTT TCATTTGCAC TGGCCCATCC GGTAAGCCTC GGGGCTCCGG CATGTGAGGG
     2341 TTCGACTCCC TCAGCAGAAA ACATGCCGTG AAAGAAAACA CCAGTTTTAG AGCTAGAAAT
     2401 AGCAAGTTAA AATAAGGCTA GTCCGTTATC AACTTGAAAA AGTGGCACCG AGTCGGTGCT
     2461 TTTTTTCTCG AGttttttta TCGAGTCTCC ATTGAAGGCC AGTGTTGTCA AAGGAACCAA
     2521 TTTCACCGTG GTGCGTGAAT TGGTCGGTGG TATCTACTTT GGTGAGAGAC AAGAACAGGA
     2581 AGAGTCTGCC GACGGTCAGA GCGCTTGGGA CACCGAGAAG TACAGTGTCT CTGAAGTTGA
     2641 AAGAATCACC AGAATGGCTG CCTTCATGGC TTTGCAGCAC AACCCTCCTT TGCCCATCTG
     2701 GTCCTTAGAC AAGGCCAACG TGCTTGCCTC ATCTAGATTG TGGAGAAAGA CGGTCGACAA
     2761 GGTGATGAGC GAGGAGTTCC CTCAATTGAC CATCAACCAC CAGCTCATTG ACTCTGCTGC
     2821 CATGATTTTG GTGCAGAGCC CAACTAAATT GAACGGTGTT GTGATCACGT CAAACATGTT
     2881 TGGTGATATT ATTTCCGACG AGGCTTCCGT GATCCCTGGC TCCTTGGGTC TTTTACCTTC
     2941 TGCATCTTTG GCTTCATTAC CAGATACAAA TAAGGCGTTT GGTTTGTACG AACCATGCCA
     3001 CGGTTCGGCT CCGGACTTGC CAGAAAACAA GGTCAACCCT GTCGCCACGA TTTTGTCGGC
     3061 CGCTATGATG TTGCGCTTGT CATTAGACTG CCTCAGAGAG GCAGAGGCCT TGGAGAAGGC
     3121 TGTTCGTAAC GTTTTGGACT CTGGTATTCG AACCGCAGAT TTGAAGGGCT CAAGTTCCAC
     3181 TCAGGAAGTG GGCGATGCCA TTGCTGCTGA GGTAAAGAAG CTTTTGCAGA AATAACGGTG
     3241 TTTAAACCCC AGCGCCTGGC GGGcgctcac aattccacac aacatacgag ccggaagcat
     3301 aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc
     3361 actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg
     3421 cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct
     3481 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
     3541 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
     3601 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
     3661 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
     3721 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
     3781 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
     3841 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
     3901 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
     3961 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
     4021 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt
     4081 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
     4141 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
     4201 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
     4261 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
     4321 c
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